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本研究旨在建立草原革蜱和边缘革蜱的分子生物学鉴定方法,并探讨其系统发生关系。在新疆从动物体表采集寄生蜱,形态学鉴定后,PCR扩增获得2种革蜱的16S rRNA及线粒体色素氧化酶亚基Ⅰ(COⅠ)基因序列,测序后进行同源性分析。用Mega 5.0和Mrbayes 3.2软件分别构建系统进化树,草原革蜱16S rRNA序列与GenBank数据库中已知草原革蜱16S rRNA序列聚类,而边缘革蜱COⅠ序列与GenBank数据库中已知边缘革蜱COⅠ序列聚类,与形态学鉴定结果一致。本研究结果表明,在传统形态学分类的基础上,结合分子生物学鉴定方法能简易、准确鉴定草原革蜱和边缘革蜱。 相似文献
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将实验室培养的小亚璃眼蜱（Hyalomma anatolicum anatolicum)和亚洲璃眼蜱（Hyalomma asiaticum asiaticum)清洁幼虫和若虫饲喂于感染环形泰勒虫的牛体，收集饱血幼虫和若虫，用所孵化的若虫和成虫感染试验牛。结果显示，小亚璃眼蜱幼虫、若虫阶级感染，所发育之若虫、成虫均可传播环形泰勒虫；而亚洲璃眼蜱各阶级的传播试验结果均为阴性。 相似文献
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《中国兽医学报》2015,(12)
通过对璃眼蜱形态学及璃眼蜱属特异性基因(ITS-2)和其携带病原基因(Tams1)PCR扩增及其PCR产物的克隆、测序、同源性比较等,鉴定和检测采自新疆吐鲁番地区疑似牛体表璃眼蜱(n=50)及其携带病原状况。结果显示,采集的蜱虫经形态学鉴定均为小亚璃眼蜱,PCR扩增的璃眼蜱种属特异性ITS-2基因和环形泰勒虫Tams1基因序列大小分别为491 bp和534 bp。本试验获得的ITS-2序列与Gen Bank中已记载的小亚璃眼蜱ITS-2序列一致,与形态学鉴定结果相符,故这些璃眼蜱属于小亚璃眼蜱种(Hyalomma anatolicum)。在检测的50只璃眼蜱中,携带牛环形泰勒虫的有3只,阳性率为6%(3/50),并发现所扩增的携带病原目的基因序列与已登录记载的牛环形泰勒虫基因序列相似性为99%。结果表明,在传统形态学分类的基础上,借助分子生物学方法对璃眼蜱种属鉴定及无典型硬蜱种的鉴定具有简易、准确度高等特点,而且在璃眼蜱种属确定的基础上分离其携带的病原更有实际意义。 相似文献
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为了解春季新疆吐鲁番市牛体表蜱寄生情况以及蜱种类和优势种群,选取艾丁湖镇牛养殖规模较大的5个村为试验点,采集牛体表吸血蜱虫,采用形态学鉴定方法进行蜱种属鉴定,采用SPSS 26.0 软件统计分析蜱种优势度及感染情况。结果显示：在100头牛体表共采集吸血蜱虫1 362只,经形态学鉴定,均为硬蜱科璃眼蜱属的亚洲璃眼蜱和小亚璃眼蜱。小亚璃眼蜱数量居多,占58.81%（801/1 362）;蜱分布范围广,在每个村均有分布。大庄子村分布的蜱虫数量最多,占30.03%（409/1 362）;西热木村最少,占8.44%（115/1 362）。小亚璃眼蜱在琼库勒村的相对优势度（Dr）最高,为68.92%（173/251）,在花园村最低,为47.27%（191/404）;亚洲璃眼蜱在花园村的Dr最高,为57.27%（213/404）,在琼库勒村最低,为31.08%（78/251）;小亚璃眼蜱在大庄子村的蜱指数（Im）最高,为12.75,而亚洲璃眼蜱在花园村最高,为10.65。运用SPSS 26.0 软件对不同试验点两蜱种的Dr进行卡方检验,结果小亚璃眼蜱在各试验点间没有显著性差异（P＞0.05）,而亚洲璃眼蜱存在显著性差异（P＜0.05）。结果表明：该地区春季主要流行小亚璃眼蜱和亚洲璃眼蜱,且分布广泛,牛体表蜱感染较为严重。因此,春季应针对性加强璃眼蜱的驱虫工作,以降低人畜感染相关虫媒病原的风险。 相似文献
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寄生虫玻片标本制作是做好寄生虫形态观察和分类鉴定的重要手段和方法,科学的染色方法是制作高质量玻片标本的前提和基础.为了建立一种快速、简单、方便且效果良好的制作残缘璃眼蜱玻片标本的方法,本试验用硼砂卡红对残缘璃眼蜱标本进行染色,采用6种浓度的卡红染液与不染色进行染色效果对比,由此选择一种标本清晰度高、对比性强且状态易于识别的染色浓度.结果表明,80％硼砂卡红染液制作残缘璃眼蜱时,玻片标本轮廓结构最清晰,颜色鲜艳明亮,染色后虫体整体为粉红色,假头和躯体界限分明,可作为残缘璃眼蜱玻片标本染色的条件. 相似文献
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新疆尉犁县某养殖场塔里木马鹿体表蜱和游离蜱的种类鉴定及分子遗传特征 《畜牧与饲料科学》2023,44(1):17-23
[目的] 了解塔里木马鹿体表蜱及其圈舍环境游离蜱种类及分子遗传特征。[方法] 收集新疆尉犁县某规模化养殖场塔里木马鹿体表寄生和圈舍游离蜱共69只,使用体视显微镜对其进行形态学观察和种类初步鉴定;基于蜱线粒体SSU rRNA基因位点和COX I基因位点对其DNA进行PCR扩增,通过序列比对数据鉴定其种类,利用生物信息学方法解析其分子遗传特征。[结果] 经形态学观察,鉴定69只蜱均属璃眼蜱属,雌蜱和雄蜱分别有37只和32只。基于SSU rRNA基因位点,对成功获得的63只蜱序列进行比对,鉴定出18只为残缘璃眼蜱,均为同一序列型,命名为SCtype1（n=18）;45只为亚洲璃眼蜱,具有4种序列型,分别命名为SYtype1（n=3）、SYtype2（n=40）、SYtype3（n=1）和SYtype4（n=1）。基于COX I基因位点,对成功获得的64只蜱序列进行对比,鉴定出24只为残缘璃眼蜱,具有2种序列型,分别命名为CCtype1（n=22）和CCtype2（n=2）;40只为亚洲璃眼蜱,具有5种序列型,分别命名为CYtype1（n=25）、CYtype2（n=7）、CYtype3（n=4）、CYtype4（n=3）和CYtype5（n=1）。种系进化分析显示,在SSU rRNA基因位点,亚洲璃眼蜱序列单独形成一个亚群分支,与其他璃眼蜱属蜱的序列形成一个大的亚群分支;在COX I基因位点,亚洲璃眼蜱序列和残缘璃眼蜱序列单独形成一个亚群分支,与其他属蜱的序列形成不同的亚群分支。[结论] 新疆尉犁县塔里木马鹿体表蜱与游离蜱种类为亚洲璃眼蜱和残缘璃眼蜱。研究结果为鹿科动物寄生蜱的种类分布调查和遗传进化特征分析提供了基础数据。 相似文献
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试验旨在建立拉合尔钝缘蜱的分子生物学鉴定方法。从新疆地区绵羊体表采集寄生蜱,借助体视显微镜和电子显微镜对其进行形态学特征的准确鉴别,初步筛选出疑似拉合尔钝缘蜱。经PCR扩增、测序获得其18S rDNA、16S rDNA及线粒体色素氧化酶亚基Ⅰ基因（cytochrome oxidase Ⅰ,COⅠ）序列,结合GenBank数据库中参考序列,比对分析同源性并构建系统进化树,进行遗传距离评估。本研究全面、清晰地揭示了拉合尔钝缘蜱卵圆形背板、背部圆形盘窝等显微形态特征的细节。同时,在基于16S rDNA与COⅠ序列的邻位相接系统进化树中,拉合尔钝缘蜱分布于两大进化枝之一的软蜱科,同种聚类良好,且拉合尔钝缘蜱种内K2P遗传距离为0~0.3%,本研究方法可快速、准确鉴定拉合尔钝缘蜱物种。 相似文献
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小亚璃眼蜱对豚鼠免疫细胞的影响陆家海，程维兴，张守竹，赵瑞新，卢开柏，李德昌，郭固（兰州军区乌鲁木齐总医院乌鲁木齐８３００００）蝉是一些重要病原的传播媒介和保虫宿主，就黄病毒属的７０多种病毒，几乎有一半与人的疾病有关，而这些病毒大部分则由节肢动物坤来... 相似文献
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A total of 2388 cattle and 442 shelters, from two provinces (Elazig and Malatya) endemic for tropical theileriosis in the east of Turkey, were studied for Hyalomma tick populations from July 1993 to July 1995 in Elazig and from May 1998 to January 1999 in Malatya. Four thousand five hundred and eighty one of 7455 Hyalomma ticks were collected from cattle, the other ticks (2874) were collected from shelters. All of the ticks collected from shelters were Hyalomma anatolicum anatolicum. Two thousand eight hundred and ninety five (63.1%) of 4581 Hyalomma ticks collected from cattle were H.a. anatolicum. 23.8% (1047/4581), 11.7% (536/4581) and 0.6% (3/4581) of Hyalomma ticks were Hyalomma anatolicum excavatum, Hyalomma detritum and Hyalomma marginatum marginatum, respectively. A total of 5909 Hyalomma adult ticks collected from cattle (3362/5909) and shelters (2447/5909) were dissected and salivary glands were stained with Methylgreen/Pyronin method. Thousand one hundred and fifty (46.9%) of 2447 H.a. anatolicum collected from shelters and 412 (19.1%) of 2147 H.a. anatolicum collected from cattle were positive for Theileria infection. Twenty (2.4%) of 820 H.a. excavatum and 23 (4.6%) of 495 H. detritum collected from cattle were positive. The mean number of infected acini per infected male and female ticks collected from cattle were 11.3 and 22.4 in H.a. anatolicum, 4 and 6.8 in H.a. excavatum, 17.9 and 18.3 in H. detritum, respectively. In H.a. anatolicum collected from shelters, the above rates were 11.8 and 17.6 in male and female ticks, respectively. The prevalence and intensity of Theileria infection was greater in female ticks than in males. 相似文献
12.
Sayin F Karaer Z Dincer S Cakmak A Inci A Yukari BA Eren H Vatansever Z Nalbantoglu S Melrose TR 《Veterinary parasitology》2003,113(2):115-121
In this comparative study unfed nymphs of four Hyalomma tick species (Hyalomma anatolicum anatolicum, Hyalomma anatolicum excavatum, Hyalomma detritum and Hyalomma marginatum marginatum) were allowed to engorge on calves experimentally infected with Theileria annulata. The infection prevalence in the salivary glands of the adult female and male ticks of each Hyalomma species used in the study were assessed. The infection prevalence with T. annulata was high and did not vary markedly in the four Hyalomma tick species. The mean number of infected acini per tick in female and male ticks was different with female ticks having higher numbers of infected acini than the male ticks. The sex difference was more significant between H.a. anatolicum and H.a. excavatum than between H. detritum and H.m. marginatum. This study clarifies the roles of four Hyalomma tick species, and their sex, in the development of T. annulata. 相似文献
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Experiments were undertaken to determine the mode of transmission to cattle of an unnamed Babesia sp. by Hyalomma anatolicum anatolicum, Hyalomma detritum, Hy. rufipes koch, Haemaphysalis longicornis and Boophilus microplus. The unnamed Babesia species designated Babesia U sp. was isolated by infesting cattle with nymphs from female Hy. a. anatolicum ticks collected from Xinjiang province. Adults of laboratory reared Hy. a. anatolicum, Hy. detritum and Hy. rufipes koch were infected with Babesia U sp. by feeding on infected cattle, isolated with nymphal ticks of Hy. a. anatolicum derived from females collected from field. The experiments revealed that Hy. a. anatolicum was capable of transmitting Babesia U sp. transovarially in larval (2 of 4 calves), nymphal (6 of 6 calves) and adult (3 of 8 calves) stages, with prepatent periods of 16, 12, and 8 days, respectively, and that this Babesia was also transovarially transmitted by both the nymphal and adult stages of Hy. detritum and Hy. rufipes. Attempts to transmit this Babesia U sp. transovarially with Hae. longicornis and B. microplus, and transstadially with Hyalomma spp., were carried out, and the results proved to be negative. 相似文献
14.
A survey was carried out to investigate the prevalence of hard tick species (Acari: Ixodidae) on cattle and sheep southeast
of Iran. A total of 972 ticks were collected from 280 infested cattle and 1,207 ticks were collected from 632 infested sheep
during activating seasons of ticks in 2008–2009. The species collected from cattle were Hyalomma marginatum marginatum (50.92%), Hyalomma anatolicum excavatum (25.61%), Hyalomma anatolicum anatolicum (8.12%), Hyalomma asiaticum asiaticum (1.85%), and Rhipicephalus sanguineus (13.47%) while the species collected from sheep were R. sanguineus (36.37%), H. marginatum marginatum (30.65%), H. anatolicum excavatum (19.05%), H. asiaticum asiaticum (10.52%), Hyalomma detritum (3.14%), and Dermacentor marginatus (0.24%). The results show that, H. marginatum marginatum, H. anatolicum excavatum, and R. sanguineus are dominant tick species in the surveyed area. 相似文献
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利用蜱传播试验确定小亚璃眼蜱对中国新分离的牛的巴贝斯虫未定种和环形泰勒虫的传播能力与传播方式，进而明确其在中国牛梨形虫病传播中的流行病学意义。试验结果表明：小亚璃眼蜱可在雌虫阶段受到巴贝斯虫未定种的感染，并可在次代若虫（2／2）和成虫（3／3）阶段将病原传播给试验牛；小亚璃眼蜱幼虫和若虫吸入环形泰勒虫，饱血脱落的幼虫和若虫所蜕化发育的饥饿若虫（2／2）和成虫（2／2）均可将病原传递给敏感动物；感染巴贝斯虫未定种的小亚璃眼蜱饱血雌虫所孵育出的次代幼虫和若虫仍可被环形泰勒虫感染，所发育出的若虫（2／2）和成虫（2／2）可在一次传播试验中将环形泰勒虫和巴贝斯虫未定种同时传播给试验动物。 相似文献
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S Kumar J K Kondal 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1999,46(6):405-410
During feeding of ticks of the species Hyalomma anatolicum anatolicum, most of the proteins in salivary gland extracts (SGEs) remained unchanged from the unfed to the fully fed state (from day 1 to day 7 of the experiment), as revealed by SDS-PAGE. However, a 45-kDa protein band disappeared and 26-, 32- and 33-kDa bands appeared when feeding commenced. Some of the protein bands changed their intensity. When probed with anti-H. anatolicum anatolicum hyperimmune sera, transblotted SGE proteins of unfed H. anatolicum anatolicum and Hyalomma dromedarii revealed two common bands of 105 and 80 kDa. A 36-kDa protein band present in H. anatolicum anatolicum SGE could not be detected in H. dromedarii. None of these proteins were detected in partly fed Boophilus microplus when probed with anti-H. anatolicum anatolicum hyperimmune serum. This H. anatolicum anatolicum specific 36-kDa protein was strongly recognized throughout feeding, and thus may be an immunogen of importance for the development of an H. anatolicum anatolicum specific serodiagnostic assay. 相似文献
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石河子地区宠物犬蜱的种类鉴定及其携带布鲁氏菌的检测 总被引：1，自引：0，他引：1
为了解新疆石河子地区宠物犬体表寄生蜱的种类及蜱携带布鲁氏菌情况,本试验在形态学分类的基础上,基于线粒体基因12S rRNA和细胞色素氧化酶Ⅰ（COⅠ）对宠物犬体表采集寄生蜱进行分子生物学检测,使用DNAMAN软件比对分析序列同源性,并应用序列分析软件Mega 7.0邻接法构建蜱种系统发育进化树,分析蜱种的遗传进化关系;基于布鲁氏菌外膜蛋白Omp22基因对宠物犬体表寄生蜱进行布鲁氏菌PCR检测,确定宠物犬蜱布鲁氏菌携带情况。结果显示,宠物犬体表寄生的436只蜱的形态学与线粒体基因（12S rRNA和COⅠ）分子生物学鉴定结果一致,均为新疆优势蜱种之一——图兰扇头蜱（Rhipicephalus turanicus）。基于12S rRNA基因构建的蜱种系统发育树显示,本试验所得宠物犬体表寄生图兰扇头蜱序列与GenBank中已知图兰扇头蜱的序列聚为一大支。基于布鲁氏菌Omp22基因的巢式PCR扩增结果显示,宠物犬体表寄生图兰扇头蜱携带布鲁氏菌的阳性率为4.82%（21/436）,且同源性为100%。BLAST比对显示,本试验所得宠物犬图兰扇头蜱携带的布鲁氏菌与新疆流行株YC31（GenBank登录号：MK201679.1）、QH5（GenBank登录号：MK201678.1）、EM3（GenBank登录号：MK201677.1）和ML9（GenBank登录号：MK201676.1）的同源性均为100%。本研究通过形态学及分子生物学探讨了新疆石河子地区宠物犬体表寄生蜱的种类及携带布鲁氏菌基本情况,为宠物犬体表寄生蜱的种类及蜱传疾病的监测和控制等研究工作提供了基础资料。 相似文献
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Razmi GR Ebrahimzadeh E Aslani MR 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(6):309-310
The aim of this study was to determine the population of ticks in infected cattle and to identify the tick vectors of bovine theileriosis in an endemic area of Iran from 1998 to 1999. A total of 120 suspected cattle suffering from theileriosis were clinically examined and investigated for the presence of Theileria annulata in blood smears and the presence of any tick species on the body of cattle. In this study, 680 ticks were collected from 107 cattle infected with T. annulata. The prevalence of ticks infesting cattle was 92.35% Hyalomma anatolicum excavatum, 5.14% H. marginatum marginatum, 1.17% H. asiaticum asiaticum and 1.32% Rhipicephalus sanguineus. The examination of 510 tick salivary glands revealed that 51% of H. a. excavatum and 1.3% of H. a. asiaticum were infected with sporozoites of T. annulata. 相似文献
19.
亚洲璃眼蜱唾液腺一新功能基因的克隆与测序 总被引：1，自引：0，他引：1
HaB1是亚洲璃眼蜱雌成蜱唾液腺差异表达基因文库中的一个片段,根据其序列设计引物HaB1-GSP1和HaB1-GSP2,以唾液腺总RNA为模板,RACE法扩增获得HaB1的未知3′-末端。测定该末端序列,进行序列拼接,设计全长引物5-′CCAGTCCGAAGGAAGGGCG-3′和5-′TCTC-CGGGCACGTGAAGTGTC-3′。以cDNA第一链为模板,扩增获得基因全长。经核查表明,该基因为一新基因(登录号AY803896)。 相似文献