营养元素对木霉菌防治甜瓜枯萎病效果的影响   总被引：13，自引：1，他引：13  

庄敬华  高增贵  刘限  陈捷  杨宇 《植物保护学报》2004,31(4):359-364

为明确各种营养元素在木霉菌防治甜瓜枯萎病生防体系中的作用,研究了9种营养元素铜、锌、铁、硼、钼、钙、锰、镁及钾对甜瓜枯萎病菌菌丝生长、木霉菌T23菌丝生长及产孢量、甜瓜种子萌发及根系生长的影响.结果表明:10～1000μg/mL的硫酸铜、硫酸锌、硫酸亚铁及硼酸对镰孢菌菌丝生长有较强的抑制作用,而对木霉菌的抑制作用远小于镰孢菌.适量加入钼酸铵、硫酸亚铁、硫酸钙及磷酸二氢钾有利于木霉菌菌丝生长及孢子形成,对甜瓜种子萌发及胚根生长也有较强的刺激作用.木霉菌结合钼酸铵、硫酸锰和硫酸钙处理土壤能显著降低甜瓜枯萎病的病情指数,相对防效分别为73.95%、73.77%和71.36%.  相似文献   

小麦叶锈菌生理小种MFR的分子鉴定研究   总被引：2，自引：0，他引：2  

蒲志刚  刘太国  张敏  陈万权 《植物病理学报》2004,34(5):449-454

 用AFLP方法对来自中国和墨西哥的23个小麦叶锈菌生理小种进行分析,共筛选了64对引物,获得一对引物(M₀₅/E₀₃)可在MFR小种中扩增出一条特异性DNA片段,进行回收、克隆、测序,结果表明该片段具有325个碱基。根据特异性片段序列设计出SCAR标记引物,对60个叶锈菌生理小种分离物进行回检结果表明,研制的SCAR标记能够准确区分MFR生理小种。本实验结果为小麦锈菌生理小种分子检测体系的建立奠定了基础  相似文献   

稻-鸭生态系统中二化螟消长动态研究   总被引：8，自引：1，他引：8  

刘小燕  杨治平  黄璜  胡立冬  刘大志  陈艳芬 《中国植保导刊》2004,24(12):9-12

2003年4～10月,在稻鸭共栖田中,对二化螟发生规律的系统研究结果表明,稻田养鸭能显著减轻二化螟的为害。与对照相比,中稻田养鸭区二化螟为害株率减少13.4%～47.1%;晚稻田减少62.2%。稻鸭共栖能减少化学农药的使用量,具有较好的经济、社会和生态效益。  相似文献   

Effect of Epstein-Barr virus latent membrane protein 1 on expression of P53 protein in nasopharyngeal carcinoma cells     

ZHANG Su-zhen  HUANG Pei-chun  XU Yong  CHEN Jin  CAI Kang-rong  HUANG He 《园艺学报》2004,20(9):1646-1649

  相似文献   

Expression of CD44s in lung cancer and its significance     

WANG Xiao-mei  CHENG Zhi-qiang  WANG Ling  SU Xue-jin  CHEN Zhuo-huai 《园艺学报》2004,20(8):1489-1491

AIM: To investigate expression of CD44s in lung cancer and it's clinical significance. METHODS: A total of 117 primary lung cancer from patients were examined for CD44s expression by immunohistochemical staining. RESULTS: CD44s mostly expressed in non-small cell lung cancer (NSCLC) but not in small ecll lung cancer (SCLC), and squamous cell carcinoma(SCC) showed much stronger expression of CD44s than adenocarcinoma(ADC)(P＜0.05). In comparison of the lung cancer with/ without lymph node metastasis, the latter showed stronger expression of CD44s(P＜0.01). According to TNM, there was a distinct statistic difference between early stage and advanced stage(P＜0.05). CONCLUSION: CD44s might be a better indicant in histological classification of lung cancer, lymph node metastasis, clinical stage and prognosis.  相似文献   

Characteristics in the secretion of liver TNF-α induced by infusion of LPS into veins in goats     

LIU Wei-min  WU Feng-lin  MAO Xin-zhi  CHEN Wei-hua 《园艺学报》2004,20(9):1706-1709

AIM: To study the role of liver in immune regulation in experimental endotoxemia. METHODS: 17 castrated male goats were subjected to simultaneously installing catheters in jugular, hepatic and portal veins by surgery. Four days later, lipopolysaccharide (LPS) was infused in term of three groups as followings: In group ①, LPS of 20 EU (endotoxin unit, EU)·kg^-1 was infused into portal vein; In group ②, LPS of 20 EU·kg^-1 was infused into jugular vein and LPS of 1 500 EU·kg^-1 infused into jugular vein in group ③. Before and after infusion, blood samples were collected from the three veins through the catheters for 8 h.The plasma levels of TNF-α were measured by RIA. RESULTS: In group ①, the plasma TNF-α levels of hepatic and portal vein rose to peak value at 5 h, but that of the jugular vein did not changed. In group ②, the plasma TNF-α levels in hepatic vein rose to peak value at 3 h. The TNF-α levels of jugular vein rose to peak value at 1 h and the one in portal vein enhanced continuously between 0-8 h. In group ③, the plasma TNF-α levels in jugular, hepatic and portal vein rose to significant peaks at 1 h simultaneously. CONCLUSION: During experimental endotoxemia,liver showed different dynamic characteristics in TNF-α secretion according to the pathway and doses of LPS delivery.  相似文献   

Expression of cyclooxygenase-2 in IL-1β-stimulated mesangial cells is medicated by NF-κB/IκB signal pathway     

DING Gui-xia  ZHANG Ai-hua  HUANG Song-ming  WU Yuan-jun  FEI Li  GUO Mei  CHEN Rong-hua 《园艺学报》2004,20(10):1754-1758

AIM: To investigate the role of NF-κB/IκB signal pathway in the regulation of cyclooxygenase-2 (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE₂ concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-κB and degradation of IκB. RESULTS: IL-1β significantly upregulated COX-2 expression and PGE₂ production in HMC. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκB α and IκB β were observed in IL-1β-induced HMC. CONCLUSION: Expression of COX-2 in IL-1β-induced HMC is mediated by NF-κB/IκB signal pathway.  相似文献   

我国近年来水稻重大病虫害可持续控制技术重要研究进展——非化学控害技术研究   总被引：9，自引：1，他引：9  

何忠全  毛建辉  张志涛  禹盛苗  范永坚  俞晓平  黄炳超  陈其志 《植物保护》2004,30(2):23-27

针对水稻病虫害防治中长期存在控害保产与生产无公害稻米矛盾比较突出的问题,研究提出了几种效果显著的非化学控害增产技术。其中,利用水稻遗传背景、对病虫抗感水平等差异显著的水稻品种多样性种植,控制稻瘟病的效果达42.12％～76.68％,抑制白背飞虱若虫数量增长效果明显,增产糯稻或优质稻600～1050 kg／hm²,平均增收约1 500元／hm²;稻鱼共育控制稻飞虱的效果为63.77％～86.89％,对纹枯病病株抑制率70.52％,控制稻田杂草效果为89.57％,平收获鲜鱼319.5～1 177.5 kg／hm²,水稻产量比对照区增产7.05％～10.11％;稻鸭共育控制稻飞虱效果63.73％～77.18％,控制稻螟效果30.11％,控制纹枯病效果19.33％～67.03％,对稻田杂草控制效果91.96％,减少施肥30.6％、农药59.3％,减少投入1 987.05元／hm²。  相似文献   

不同柑桔品种对柑桔溃疡病抗病能力的测定   总被引：5，自引：0，他引：5  

张戈壁  阳廷密  李喜庆  陈竹生 《植物保护学报》2004,31(2):221-222

柑桔溃疡病为国内外检疫对象,危害柑桔苗木、幼树和成年树的叶片、果实、树梢等,引起落叶、落果、枯梢、削弱树势,是柑橘的重要病害.不同的柑桔品种对该病的抗病性差异程度较大,为了明确不同种类的柑桔品种对该病的抗病性,我们进行了柑桔不同品种对其抗性能力的测定.  相似文献   

导致一例强沙尘暴的若干天气因素的观测和模拟研究     

姜学恭  沈建国  刘景涛  陈受钧 《干旱区资源与环境》2004,(Z1)

利用常规观测资料和数值模拟方法对影响2001年4月6-8日强沙尘暴天气发生的若干天气因素进行了研究。结果表明在本次“锋面气旋型”沙尘暴过程中,气旋冷锋是引发强沙尘暴的主要系统,冷锋过境对沙尘暴的触发作用远强于气旋发展。冷锋的动力学特点决定其常与强沙尘暴伴随。影响沙尘暴的诸多天气因素中,影响地面大风形成的有效动量下传机制为高空急流下沉支使高空动量下传至对流层中层,其下方形成的深厚混合层使这一动量继续下传到地面。“混合层”从本质上反映了有利沙尘暴形成的大气层结特征,深厚混合层的是否形成很大程度决定着沙尘暴的强度。本次过程深厚混合层的形成是长时间地面加热的结果,这也是特强沙尘暴仅发生在内蒙古中部偏北地区而不是下垫面条件更为适宜的内蒙古西部的原因。混合层空气的平流对内蒙古中部偏北地区深厚混合层的形成具有相当的贡献,其影响程度与地形分布密切相关。地面热通量试验表明地面加热对冷锋过境时上升运动的强度、深厚混合层形成、高空动量下传等具有重要影响,并因此影响沙尘暴强度。  相似文献