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41.
42.
多重PCR检测猪瘟病毒、猪细小病毒、猪伪狂犬病病毒 总被引:26,自引:0,他引:26
根据猪瘟病毒(CSFV)、猪细小病毒(PPV)和猪伪狂犬病病毒(PRV)3种病原体的基因保守序列,分别设计了与CSFV的E2基因、PPV的VP2基因和PRV的gⅡ基因序列互补的3对引物。用这3对引物对人为混合的样品中的PPV和PRV的DNA模板及CSFV反转录后的cDNA模板进行多重PCR扩增和多重PCR反应奈件的优化,结果同时得到3务与试验设计相符的288bp(CSFV)、575bp(PPV)700bp(PRV)特异性条带;敏感性检测结果表明,该多重PCR可以检测到14.5ng/L的CSFV、27.1pg/L的PPV、31.4ng/L的PRV的核酸模板。 相似文献
43.
44.
Osteomyelitis caused by Cryptococcus neoformans was diagnosed in a three-year-old male Irish wolfhound. The case details and radiological findings of radiolucency and periosteal reaction of the left third metatarsal bone had lead to an initial diagnosis of a malignant bone tumour. Biopsy revealed a fungal granuloma and culture identified the causal organism. A four month course of ketaconazole was instigated and the lameness resolved. Twelve months after the end of treatment there was no recurrence of the problem. 相似文献
45.
R F Nachreiner S J Oschmann L E Edqvist J I Richards 《American journal of veterinary research》1992,53(7):1085-1089
Five studies were performed to determine factors affecting progesterone concentration in skim milk. Results of the first study indicated that progesterone concentration was higher in skim milk of samples kept 16 hours in an ice bath (0 C) than of those left at room temperature (21 C). In the second study, this temperature effect was found to be reversible, with skim milk progesterone concentration increasing when whole milk samples were cooled prior to centrifugation. In the third study, [3H]-labeled progesterone was used to determine the relationship between fat content of foremilk (the first milk obtained from the teats), midmilk (milk obtained midway through milking), and strippings (milk obtained immediately after milking machines have been removed) samples and temperature (4 C and 21 C) on the percentage of progesterone in the skim milk fraction. The relationship between percentage of butterfat and percentage of progesterone in skim milk was linear when the log of these variables was used for calculations. In the fourth study, assayable progesterone in the skim milk fraction of foremilk, midmilk, and strippings was affected by temperature. In the fifth study, a multiple-regression procedure was used to determine the amount of variation in percentage of radioactive progesterone in the skim milk fraction. Independent variables (whole milk butterfat and temperature of incubation [1, 3, 13, 22, 37, and 50 C]) and the natural log of each variable, were entered into a stepwise multiple-regression analysis.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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The case records of 149 dogs examined from 1985 to 1989 with clinical signs of nasal disease were reviewed. Gross rhinoscopy was performed in 119 dogs, and rhinoscopy-assisted pinch biopsy was performed in 109. Rhinoscopy was performed by use of a 2.7-mm rigid fiberoptic endoscope. Mucosal biopsy specimens were obtained with rhinoscopic guidance by use of a 2 x 3-mm biopsy forcep. Gross, cytologic, and histologic findings are summarized. Ninety-four of 119 cases could be evaluated on the basis of diagnostic and follow-up criteria established by the authors. The diagnostic success rate of gross rhinoscopy with rhinoscopy-assisted biopsy was 83% (78 of 94 evaluated cases). Protracted hemorrhage was a complication in 2 of 109 cases in which rhinoscopy-assisted biopsy was performed. It was concluded that rhinoscopy with rhinoscopy-assisted biopsy contributes important diagnostic information in dogs with nasal disease without the relative invasiveness, expense, and risk of surgery. 相似文献
48.
Differences in gas exchange parameters i.e. carbon exchange rate (CER), transpiration (Tr), water vapor conductance (g) were investigated, under a controlled environment, in a semi-dwarf (SD) Triticum aestivum line, its tall (TL) near isoline and the Payne (PA) variety characterized by small leaves. The plants were maintained in: (a) optimal substrate moisture condition (CTR); (b) salinized by watering with a 0.12 m NaCl solution (SLT); (c) water stressed by withholding waterings during a period of six days (STR); (d) stressed and salinized during the same period and with the same saline solution (S + S). CER and Tr were negatively affected by the stresses; SLT and S + S treatments had a higher WUE compared to their respective controls CTR and STR.
SD line had a better performance in terms of CER and WUE, particularly when water and salt stresses interacted. Leaf anatomy and assimilation/internal CO2 concentration curves contributed to explaining the SD performance. 相似文献
SD line had a better performance in terms of CER and WUE, particularly when water and salt stresses interacted. Leaf anatomy and assimilation/internal CO
49.
阐述了某工程由于施工原因,导致预应力混凝土工字形屋面梁强度不足的预制构件的检验鉴定过程和方法。采用回弹法推定混凝土实际强度;通过静载试验测试构件的实际挠度、抗裂和承载能力。分析了导致混凝土强度不足的原因。 相似文献
50.
Pilot study of the effect of acemannan in cats infected with feline immunodeficiency virus. 总被引:7,自引:0,他引:7
K M Yates L J Rosenberg C K Harris D C Bronstad G K King G A Biehle B Walker C R Ford J E Hall I R Tizard 《Veterinary immunology and immunopathology》1992,35(1-2):177-189
Acemannan, a complex carbohydrate shown to stimulate interleukin-1, tumor necrosis factor alpha and prostaglandin E2 production by macrophages, has also demonstrated antiviral activity in vitro against human immunodeficiency virus, Newcastle disease virus and influenza virus. A pilot study was undertaken to determine acemannan's effect in 49 feline immunodeficiency virus (FIV) infected cats with clinical signs of disease (Stage 3, 4 or 5), 23 of which had severe lymphopenia. Cats received acemannan either by intravenous (Group 1) or subcutaneous (Group 2) injection once weekly for 12 weeks, or by daily oral (Group 3) administration for 12 weeks. Upon entry into the study, cats were randomly assigned to one of the three groups. Laboratory analyses were performed at the beginning of the study and at Weeks 6 and 12. Cats were allowed to continue with a predetermined maintenance regimen of acemannan after completing the 12-week study. Thirteen cats died during the course of treatment. Upon necropsy, the most frequent histopathologic findings were neoplastic, kidney and pancreatic disease. Friedman's two-way ANOVA test showed no significant differences in efficacy among groups administered acemannan by the different routes. Therefore, groups were combined and a signed-ranks test was used to determine changes over time. A significant increase was seen in lymphocyte counts (P < 0.001). Neutrophil counts decreased significantly (P = 0.007), as did incidence of sepsis (P = 0.008). When cats entering with lymphopenia were analyzed separately, a much greater increase in lymphocyte counts was noted (235%) compared with non-lymphopenic cats (42%). A survival rate of 75% was found for all three groups. Thirty-six of 49 animals are alive 5-19 months post-entry. These results suggest that acemannan therapy may be of significant benefit in FIV-infected cats exhibiting clinical signs of disease. 相似文献