Production and characterization of monoclonal antibodies against formalin-inactivated Nipah virus isolated from the lungs of a pig     

Imada T  Abdul Rahman MA  Kashiwazaki Y  Tanimura N  Syed Hassan S  Jamaluddin A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(1):81-83

Eight clones of monoclonal antibodies (Mabs) to Nipah virus (NV) were produced against formalin-inactivated NV antigens. They reacted positive by indirect immunofluorescent antibody test, and one of them also demonstrated virus neutralizing activity. They were classified into six different types based on their biological properties. These Mabs will be useful for immunodiagnosis of NV infections in animals and further research studies involving the genomes and proteins of NV.  相似文献   

Characterization and molecular mapping of EMS-induced brittle culm mutants of diploid wheat (<Emphasis Type="Italic">Triticum monococcum</Emphasis> L.)     

Mohd. Javed Ansari  Rahul Kumar  Kuldeep Singh  Harcharan S. Dhaliwal 《Euphytica》2012,186(1):165-176

Diploid wheat (Triticum monococcum L, A^mA^m) is an ideal material for induced mutations which can be easily characterized and transferred to polyploid wheats. The EMS-induced brittle culm mutants, brc1, brc2, and brc3 used in the present investigation, were isolated from T. monococcum. All the brittle mutants had brittle roots, leaves, leaf sheaths, culms, and spikes, and were also susceptible to lodging. The mutants had 47–57% reduced α-cellulose in the secondary cell walls than that of T. monococcum indicating that all of them had defective synthesis of cellulose. All the mutants were monogenic recessive. Bulk segregation analysis of the mutants, using A^m genome anchored SSR markers in their F ₂ populations with T. boeoticum, located the mutants, brc1, brc2, and brc3 on chromosome 6A, 3A, and 1A of T. monococcum, respectively. Molecular analysis of the putatively linked markers showed that brc1 mapped on chromosome 6AS between Xbarc37 and Xbarc113 markers, brc2 on chromosome 3AL between Xcfd62 and Xcfa2170 markers whereas brc3 mapped on chromosome 1AL between Xgwm135 and Xwmc470 markers. Isolation and mapping of three different brittle culm mutants in wheat for the first time shows that there might be many more genes in wheat which affect synthesis and deposition of cellulose.  相似文献   

Brassinosteroid-mediated enrichment in yield attributes, active constituents and essential oil production in Mentha arvensis L.     

M. Naeem  Mohd. Idrees  M. Masidur Alam  Tariq Aftab  M. Masroor A. Khan  Moinuddin 《Russian Agricultural Sciences》2012,38(2):106-113

Recently, brassinosteroids (BRs) have emerged as a new group of growth promoting phytohormones. 28-homobrassinolide (HBR) is one of the several brassinosteroids, the role of which in enhancing growth, productivity and quality of plants, via improving various physiological processes, has been established both under stress and normal conditions. Out of a large number of essential oil bearing plants, mint (Mentha arvensis L.) constitutes the most important source of therapeutic agents used in the alternative systems of medicine. The mint plant has marvelous medicinal and aromatic values. In view of enhancing yield and quality of this medicinally important plant, a pot experiment was conducted under natural conditions of net house. The study was aimed at exploring the effect of four concentrations of HBR (10^?0, 10^?8, 10^?7 and 10^?6 M) on the performance of mint with regard to physiological attributes, herbage yield, content and yield of essential oil, and active constituents at 100 and 120 days after planting. The foliar application of HBR enhanced physiological attributes, herbage yield and the yield and content of most of the active constituents (menthol, L-methone, isomenthone and menthyl acetate) of mint at both the stages, with 10^?7 M concentration proving the best. However, the next higher concentration of HBR (10^?6 M) exhibited no further increase in the values of the attributes studied. Rather, 10^?6 M was slightly inferior to 10^?7 M concentration; but it always proved significantly better than the control.  相似文献   

Implementation of herd health program to improve survival of Boer goats in Malaysia     

Salisi MS  Saad MZ  Kasim A 《Tropical animal health and production》2012,44(2):207-211

A Boer goat breeding farm with 800 heads of breeder females, 50 breeder males, and 400 growing goats of various ages in Sabah, Malaysia was selected to study the effect of implementing herd health program. This included vaccination program against pneumonic mannheimiosis; fecal monitoring for helminthiasis, coccidiosis, and colibacillosis; and introduction of modified feeding regime comprised of day-time grazing and feeding of cut grass and supplemented feed. The herd health program was implemented in September 2007 and the impact was observed on body weight gains, body scoring, and annual mortality among adults and kids. It was found that implementation of herd health program significantly (p < 0.05) increased the average body weight gains in both adults and kids from 1.8 g per kid and 0.6 g per adult in 2006 to 3.7 g per kid and 2.2 g per adult in 2008. The percentage of adults with body scoring of <3 was significantly (p < 0.05) reduced from 82.3% in 2006 to 77.6% in 2007 and 4% in 2008. Similarly, the annual mortality rate was significantly (p < 0.05) reduced from 6.5% among kids and 58.2% among adults in 2006 to 12.1% among kids and 10.4% among adults in 2007, and to 9.1% among kids and 1.1% among adults in 2008. Therefore, it was concluded that implementation of herd health program significantly improved the survival and performance of goats.  相似文献   

Nifedipine for the Treatment of High Altitude Pulmonary Edema     

Rajesh Deshwal  Mohd Iqbal  Sidhant Basnet 《The Forestry Chronicle》2012,23(1):7-10

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Bacteriological and Molecular Detection of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus in Equines of Northern India     

Irfan Ahmad MIR  Bablu KUMAR  Anil TAKU  Farah FARIDI  Mohd. Altaf BHAT  Naseer Ahmad BABA  Tahir MAQBOOL 《Journal of Equine Science》2013,24(3):53-55

Present study was undertaken to study the prevalence of β-haemolytic streptococci in equine of northern temperate region of Jammu and Kashmir, India. One hundred and forty one samples were collected in duplicate from nasopharyngeal tract of diseased (53) and apparently healthy equine (88) for isolation and direct PCR. A total of 77 isolates of streptococci were recovered from 141 samples with an overall prevalence rate of 54.60%. Out of these 77 isolates, 52 were from diseased and 25 from apparently healthy animals. Of the 77 isolates, 4 were identified as Streptococcus equi subsp. equi, 56 as S. equi subsp. zooepidemicus and 17 as S. dysgalactiae subsp. equisimilis. Thus the overall prevalence of S. equi subsp. equi, S. equi subsp. zooepidemicus and S. dysgalactiae subsp. equisimilis was 2.83, 39.71 and 12.05% respectively. The sensitivity of the PCR for the detection of S. equi species was found higher when attempted from direct swab samples.  相似文献   

Pantoea: a newly identified causative agent for leaf blight disease in rice     

Doni  Febri  Suhaimi  Nurul Shamsinah Mohd  Mohamed  Zulqarnain  Ishak  Nazri  Mispan  Muhamad Shakirin 《植物病害和植物保护杂志》2019,126(6):491-494

Journal of Plant Diseases and Protection - Leaf blight is one of the most serious threats to rice agroecosystems worldwide, and the yield reduction due to this disease can be as high as 70%. For...  相似文献   

Contagious caprine pleuropneumonia – a comprehensive review     

Mohd. Iqbal Yatoo  Oveas Raffiq Parray  Shah Tauseef Bashir  Muheet  Riyaz Ahmed Bhat  Arumugam Gopalakrishnan 《The Veterinary quarterly》2019,39(1):1-25

Contagious caprine pleuropneumonia (CCPP) is a serious disease of goats, occasionally sheep and wild ruminants, caused by Mycoplasma capricolum subspecies capripneumoniae (Mccp). The disease is characterized by severe serofibrinous pleuropneumonia, very high morbidity (～100%), and mortality (80–100%). CCPP affects goats in more than 40 countries of the world thereby posing a serious threat to goat farming around the globe. The characteristic clinical signs of CCPP are severe respiratory distress associated with sero-mucoid nasal discharge, coughing, dyspnea, pyrexia, pleurodynia, and general malaise. In later stages, severe lobar fibrinous pleuropneumonia, profuse fluid accumulation in pleural cavity, severe congestion of lungs and adhesion formation is observed. Mycoplasmal antigen interactions with host immune system and its role in CCPP pathogenesis are not clearly understood. CCPP is not a zoonotic disease. Diagnosis has overcome cumbersome and lengthy conventional tests involving culture, isolation, and identification by advanced serological (LAT, cELISA) or gene-based amplification of DNA (PCR, RFLP, and hybridization) and sequencing. The latex agglutination test (LAT) is rapid, simple, and better test for field and real-time diagnosis applicable to whole blood or serum and is more sensitive than the CFT and easier than the cELISA. Moreover, the studies on antibiotic sensitivity and exploration of novel antibiotics (fluoroquinolones, macrolides) can help in better therapeutic management besides preventing menace of antibiotic resistance. Re-visiting conventional prophylactic measures focussing on developing novel strain-based or recombinant vaccines using specific antigens (capsular or cellular) should be the most important strategy for controlling the disease worldwide.  相似文献   

Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators,mediators and immunoglobulins in cows     

Hambali  Idris Umar  Abdullah  Faez Firdaus Jesse Bin  Bhutto  K. R.  Mohd Azmi  M. L.  Wahid  A. H.  Zakaria  Z.  Odhah  M. N.  Arsalan  M.  Muhammad  N. A.  Jefri  M. N. 《Tropical animal health and production》2019,51(4):781-789

Tropical Animal Health and Production - Mastitis is the inflammation of the mammary gland due to microbial infiltration causing a reduced mammary function. This study aims at developing a vaccine...  相似文献   

Comparative evaluation of different therapeutic protocols for contagious caprine pleuropneumonia in Himalayan Pashmina goats     

Yatoo  Mohd. Iqbal  Parray  Oveas Raffiq  Mir  Muheet  Bhat  Riyaz Ahmed  Malik  Hamid Ullah  Fazili  Mujeeb ur Rehman  Qureshi  Sabia  Mir  Masood Salim  Yousuf  Raja Wasim  Tufani  Noor Alam  Dhama  Kuldeep  Bashir  Shah Tauseef 《Tropical animal health and production》2019,51(8):2127-2137

Tropical Animal Health and Production - Therapeutic management of contagious caprine pleuroneumonia (CCPP) involves mostly the use of oxytetracycline followed by enrofloxacin and rarely tylosin. In...  相似文献