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1.
ECOLOGY OF MYCOPLASMAS IN CLINICALLY HEALTHY CATS 总被引：1，自引：0，他引：1
Mycoplasmas were isolated from various sites and organs of a series of 319 clinically healthy cats. They include M. felis, M. gateae, M. Arginini, A. laidlawii, feline ureaplasmas, M. pulmonis, M. arthritidis, and M. gallisepticum. In addition, there were 10 strains of mycoplasmas which could not be identified with the specific antiserums by growth inhibition tests. Antibody against M. felis was demonstrated by haemagglutination-inhibition and complement-fixation tests in cats which were over 6 months of age. However, no such antibody against M. felis was detected in animals which were less than 6 months old. No antibody against A. laidlawii, M. gateae, M. arginini and feline ureaplasmas was demonstrated by the same serological methods. The significance of these mycoplasmas in cats is described. 相似文献
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Canapp SO Farese JP Schultz GS Gowda S Ishak AM Swaim SF Vangilder J Lee-Ambrose L Martin FG 《Veterinary surgery : VS》2003,32(6):515-523
OBJECTIVE: To evaluate the effects of topical glycyl-L-histidyl-L-lysine tripeptide-copper complex (TCC; Iamin 2% Gel; Procyte Corporation, Redmond, WA) on healing in ischemic open wounds. STUDY DESIGN: Experimental study. SAMPLE POPULATION: Twenty-four adult male Sprague-Dawley rats. METHODS: Rats were divided into 3 groups: topical TCC, topical TCC vehicle (hydroxypropyl-methylcellulose), and no treatment (control). Six-mm-diameter, full-thickness wounds were created within an ischemic bipedicle skin flap on the dorsum of each rat. Each day, for 13 days, wound margins were traced, and the TCC and TCC vehicle groups were treated topically. Tracings were scanned, and wound perimeter and area were calculated. On days 6, 10, and 13, selected wounds were biopsied and analyzed for tumor necrosis factor alpha (TNF-alpha) and matrix metalloproteinases (MMP) 2 and 9. RESULTS: A significant decrease in wound area was seen in the TCC group, but not the vehicle group, when compared with the control group on days 3 to 5, 6 to 9, and 11 to 13 and when TCC was compared with TCC vehicle on days 3 and 9. On day 13, initial wound area had decreased by 64.5% in the TCC group, 45.6% in the vehicle group, and 28.2% in the control group. On days 6, 10, and 13, TCC-treated wounds contained significantly lower concentrations of TNF-alpha and MMP-2 and MMP-9 than control wounds. CONCLUSION: Topical TCC resulted in accelerated wound healing in ischemic open wounds. CLINICAL RELEVANCE: Topical TCC is an effective stimulant of healing of ischemic open wounds in rats and may have an application for the treatment of chronic wounds in other species. Clinical evaluation of topical TCC is warranted. 相似文献
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Doni Febri Suhaimi Nurul Shamsinah Mohd Mohamed Zulqarnain Ishak Nazri Mispan Muhamad Shakirin 《植物病害和植物保护杂志》2019,126(6):491-494
Journal of Plant Diseases and Protection - Leaf blight is one of the most serious threats to rice agroecosystems worldwide, and the yield reduction due to this disease can be as high as 70%. For... 相似文献
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Transgenic Nipponbare which over-expressed a Na+/H+ antiporter gene OsNHX1 was used to compare its growth performance, water status and photosynthetic efficiency with its wild type under varying salinity regimes. Chlorophyll content, quantum yield and photosynthetic rate were measured to assess the impact of salinity stress on photosynthetic efficiency for transgenic and wild-type Nipponbare. Effects of salinity on water status and gas exchange to both lines were studied by measuring water use efficiency, instantaneous transpiration rate and stomatal conductance. Dry shoot weight and leaf area were determined after three months of growth to assess the impacts of salinity on the growth of those two lines. Our study showed that both lines were affected by salinity stress, however, the transgenic line showed higher photosynthetic efficiency, better utilization of water, and better growth due to low transpiration rate and stomatal conductance. Reduction of photosynthetic efficiency exhibited by the wild-type Nipponbare was correlated to its poor growth under salinity stress. 相似文献
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Cellulose fibres and cellulose nanocrystals were extracted from rice husk. Fibres were obtained by submitting the industrial rice crop to alkali (NaOH) and bleaching treatments. Nanocrystals were extracted from these fibres using sulphuric acid (H2SO4) hydrolysis treatment. The material obtained after each stage of the treatments was carefully characterized and its chemical composition was determined. Morphological investigation was performed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Fourier transform infrared (FTIR) spectroscopy showed the progressive removal of non-cellulosic constituents. X-ray diffraction (XRD) analysis revealed that the crystallinity increased with successive treatments. The thermal stability of the rice husk fibres and cellulose nanocrystals was also investigated using thermogravimetric analysis (TGA). 相似文献
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TA Abdulkareem SAM Al‐Sharifi MA Ishak SM Eidan MA Alnimr CW Passavant JR Branen RG Sasser 《Reproduction in domestic animals》2011,46(3):455-462
This study was undertaken to detect pregnancy in Iraqi riverine buffalo (Bubalus bubalis) using three different methods (rectal palpation, plasma progesterone concentration and detection of the presence of pregnancy‐specific protein B (PSPB) with the BioPRYN® enzyme‐linked immunosorbent assay (ELISA) test. The aim of the study was to identify the most sensitive, early and accurate method for detecting pregnancy. Twenty‐two female riverine buffalo that were 6.0 ± 0.93 years old were used. Four blood samples per buffalo were taken via jugular venipuncture at days 22–24, 32–34, 42–44 and 58–61 post‐mating (PM) to measure the progesterone concentration (ng/ml) and to detect the presence of plasma PSPB. The rectal palpation method was employed to evaluate all buffalo on days 42–44 and 58–61 PM. The BioPRYN® test differed (p < 0.01) from the other tests with earlier accuracy for detecting pregnant and non‐pregnant buffalo. Eighty‐eight percent of pregnant and 76.9% of non‐pregnant buffalo were distinguished early (days 22–24 PM) using BioPRYN® and plasma PSPB‐ELISA level (2.09 ± 0.12 ng/ml) in relation to 66.7% and 53.9% detected using the progesterone assay at similar days (4.30 ± 0.40 ng/ml). In conclusion, these results described, for the first time, the early and accurate pregnancy detection of water riverine buffalo using BioPRYN® technology and provided the plasma levels of PSPB using an ELISA test. These findings will improve the reproductive and productive efficiency of Iraqi riverine buffalo by adapting the recent management and reproductive strategies in Iraq and in the world. 相似文献
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A. Karami A. Christianus Z. Ishak S. C. Courtenay M. A. Syed M. Noor Azlina H. Noorshinah 《Aquaculture International》2010,18(5):851-858
Triploid African catfish (Clarias gariepinus) produced through cold shock 3 min post fertilization were compared to diploid offspring of the same parentage at 66 days
of age. Triploid fish were significantly shorter (11%) and weighed less (18%) than diploids but showed no significant difference
in mortality or cannibalism, which can be an important source of losses under aquaculture conditions. Erythrocytes of triploid
fish were more oval shaped than the normal spherical shape of diploid erythrocytes, were significantly larger (38%) and had
nuclei that were significantly larger (25%) than observed in diploid fish. Erythrocyte morphological characteristics show
potential as an indicator for the detection of triploidy in African catfish. 相似文献
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In this work, hybrid composites were fabricated by hand layup method to hybridize treated Pineapple leaf fibre (PALF) and kenaf fibre (KF) in order to achieve superior mechanical properties on untreated hybrid composites. Silane treated PALF/KF phenolic hybrid composites were prepared on various fibre fraction to investigate mechanical properties and compared with untreated PALF/KF phenolic hybrid composites. The effects of silane treatment on hybrid composites were investigated by fourier transform infrared spectroscopy (FTIR) and found very effective peaks. Effects of treated hybrid composites were morphologically investigated by using scanning electron microscopy images and analysed the tensile results. Treated PALF/KF phenolic hybrid composites enhanced the flexural strength, modulus, impact strength and energy absorption while tensile strength and modulus decreased. The overall performances of 70 % PALF 30 % Kenaf hybrid composites were improved after silane treatment. Silane treatment of fibres improved the mechanical performance of hybrid composites and it can be utilized to produce components for building structure, materials and automobile applications. 相似文献
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A new flavanone derivative, malaysianone A (1), four prenylated flavanones, 6-prenyl-3'-methoxyeriodictyol (2), nymphaeol B (3), nymphaeol C (4) and 6-farnesyl-3',4',5,7-tetrahydroxyflavanone (5), and two coumarins, 5,7-dihydroxycoumarin (6) and scopoletin (7), were isolated from the dichloromethane extract of the inflorescences of Macaranga triloba. The structures of these compounds were elucidated based on spectroscopic methods including nuclear magnetic resonance (NMR-1D and 2D), UV, IR and mass spectrometry. The cytotoxic activity of the compounds was tested against several cell lines, with 5 inhibiting very strongly the growth of HeLa and HL-60 cells (IC(50): 1.3 μg/ml and 3.3 μg/ml, respectively). Compound 5 also showed strong antiplasmodial activity (IC(50): 0.06 μM). 相似文献