Experimental Transmission of VHSV Genotype IVb by Predation     

Rodman G. Getchell  Emily R. Cornwell  Geoffrey H. Groocock  Po Ting Wong  Laura L. Coffee  Gregory A. Wooster 《Journal of aquatic animal health》2013,25(4):221-229

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Veterinary Hematology: A Diagnostic Guide and Color Atlas     

Valerie Wong  Dr. Brent Hoff  Dip Tox 《The Canadian veterinary journal. La revue veterinaire canadienne》2013,54(6):609

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Fibrinous pericarditis and cardiac tamponade in a 3‐week‐old pony foal     

C. J. Alcott  J. Howard  D. Wong  J. Haynes 《Equine Veterinary Education》2013,25(7):328-333

A 3‐week‐old Pony of the Americas foal presented with a history of respiratory distress presumed to result from Actinobacillus equuli pleuropneumonia and septic arthritis. Failure of transfer of passive colostral immunity was suspected, but not confirmed, based on a history of the foal being separated from its dam shortly after parturition. Transient improvement was noted following thoracocentesis and removal of approximately 600 ml of pleural fluid but progressive clinical signs of congestive heart failure developed. Fibrinous pericarditis with evidence of cardiac tamponade was subsequently diagnosed via thoracic ultrasonography. Early clinical signs of cardiogenic shock were identified and fibrinopurulent exudate removed through a catheter placed with ultrasound guidance into the pericardium. The foal experienced cardiorespiratory arrest during the procedure and died despite resuscitative efforts. Post mortem examination identified extensive hypertrophy of the pericardium, septic arthritis, mild pleural effusion and focal bronchopneumonia. This report details the clinical evaluation, haematology, treatment and post mortem pathology of a foal with Actinobacillus equuli associated fibrinous pericarditis, as well as a brief review of cardiac tamponade.  相似文献   

In vitro antioxidant and antiproliferative activities of selenium-containing phycocyanin from selenium-enriched Spirulina platensis     

Chen T  Wong YS 《Journal of agricultural and food chemistry》2008,56(12):4352-4358

Both selenium and phycocyanin have been reported to show potent cancer chemopreventive activities. In this study, we investigated the in vitro antioxidant and antiproliferative activities of selenium-containing phycocyanin (Se-PC) purified from selenium-enriched Spirulina platensis. The antioxidant activity of Se-PC was evaluated by using four different free radical scavenging assays, namely, the 2,2'-azinobis-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) assay, 1,1-diphenyl-2-picryhydrazyl (DPPH) assay, superoxide anion scavenging assay, and erythrocyte hemolysis assay. The results indicated that Se-PC exhibited stronger antioxidant activity than phycocyanin by scavenging ABTS, DPPH, superoxide anion, and 2,2'-azobis-(2-amidinopropane)dihydrochloride free radicals. Se-PC also showed dose-dependent protective effects on erythrocytes against H 2O 2-induced oxidative DNA damage as evaluated by the Comet assay. Moreover, Se-PC was identified as a potent antiproliferative agent against human melanoma A375 cells and human breast adenocarcinoma MCF-7 cells. Induction of apoptosis in both A375 and MCF-7 cells by Se-PC was evidenced by accumulation of sub-G1 cell populations, DNA fragmentation, and nuclear condensation. Further investigation on intracellular mechanisms indicated that depletion of mitochondrial membrane potential (DeltaPsi m) was involved in Se-PC-induced cell apoptosis. Our findings suggest that Se-PC is a promising organic Se species with potential applications in cancer chemoprevention.  相似文献   

Reproducible infection model for Clostridium perfringens in broiler chickens     

Pedersen K  Bjerrum L  Heuer OE  Lo Fo Wong DM  Nauerby B 《Avian diseases》2008,52(1):34-39

Experiments were carried out to establish an infection and disease model for Clostridium perfringens in broiler chickens. Previous experiments had failed to induce disease and only a transient colonization with challenge strains had been obtained. In the present study, two series of experiments were conducted, each involving four groups of chickens with each group kept in separate isolators. A coccidial vaccine given at 10 times the prescribed dosage was used to promote the development of necrotic enteritis. In the first experiment, cultures of C. perfringens were mixed with the feed at day 9, 10, 11, and 12, and the coccidial vaccine was given at day 10, whereas in the second experiment, C. perfringens cultures were mixed with the feed at day 17, 18, 19, and 20, and the coccidial vaccine was given at day 18. Chickens were examined at day 9, 11, 12, and 15 (Experiment 1), and at day 17, 18, 20, and 24 (Experiment 2). There was no mortality in any of the groups; however, chickens in the groups receiving both coccidial vaccine and C. peifringens developed the subclinical form of necrotic enteritis, demonstrated by focal necroses in the small intestine, whereas chickens in control groups or groups receiving only coccidial vaccine or only C. perfringens cultures developed no necroses. The results underline the importance of predisposing factors in the development of necrotic enteritis.  相似文献   

Seroepidemiology and genotyping of hepatitis E virus in Singapore reveal rise in number of cases and similarity of human strains to those detected in pig livers     

Chui Ching Wong  Su Ming Thean  Youming Ng  Joanne Su Lin Kang  Tong Yong Ng  Man Ling Chau  Tse Hsien Koh  Kwai Peng Chan 《Zoonoses and public health》2019,66(7):773-782

Hepatitis E virus (HEV) causes 20 million infections worldwide yearly, of which only about 3.3 million are symptomatic. In developed Asian countries, HEV strains detected in human sera and in food sources were genetically similar, suggesting that indigenous HEV infections may be largely food‐borne. To assess the burden of hepatitis E in Singapore, we performed a seroepidemiologic study of the infection. Additionally, we carried out HEV genotyping on archived, residual HEV IgM‐positive serum samples collected between 2014 and 2016 (n = 449), and on pig liver samples (n = 36) purchased from wet markets and supermarkets. Our study shows a rise in hepatitis E incidence (IgM) from 1.7 to 4.1 cases per 100,000 resident population from 2012 to 2016 and an increase in hepatitis E IgG positivity rate among residents from 14% in 2007 to 35% in 2016. Other findings also suggest the epidemiology of hepatitis E in Singapore has shifted, from it being mainly a disease imported from the Indian subcontinent, to one that is now increasingly prevalent in our resident population. Genotypes obtained from 143 human samples identified the majority to be genotype 3 (n = 121), 21 to be genotype 1 and one to be genotype 4. Further phylogenetic analyses suggest genotype 3a to be the cause of indigenous infections in residents, which showed genetic similarity to the genotype 3a strains detected in pig livers. This link between the strains in the majority of human samples and those in pig livers consumed by the public suggests a possible food‐borne route of HEV infection in Singapore.  相似文献   

Polymerase chain reaction for antigen receptor rearrangement: Benchmarking performance of a lymphoid clonality assay in diverse canine sample types     

E. J. Ehrhart  Shukmei Wong  Keith Richter  Victoria Zismann  Carolyn Grimes  William Hendricks  Chand Khanna 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(3):1392-1402

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Discriminatory features of acute eosinophilic dermatitis with oedema (Wells‐like syndrome) and sterile neutrophilic dermatosis (Sweet's‐like syndrome) in dogs     

Charles W. Bradley  Christine L. Cain  Talia S. Wong  Jacquelyn D. Ferracone  Kyle H. Goldschmidt  Elizabeth A. Mauldin 《Veterinary dermatology》2019,30(6):517-e157

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家蚕（Bombyxmori）全基因组框架图   总被引：27，自引：0，他引：27  

夏庆友  周泽扬  鲁成  程道军  代方银  李斌  赵萍  查幸福  程廷才  柴春利  潘国庆  许金山  刘春  林英  钱吉凤  侯勇  吴正理  李关荣  潘敏慧  李春峰  沈以红  蓝希钳  袁联伟  李田  徐汉福  杨光伟  万永继  朱勇  余茂德  沈卫德  吴大洋  向仲怀  于军  王俊  李瑞强  石剑萍  李恒  李光远  苏建宁  王晓玲  李国庆  张增金  吴清发  李俊  张庆鹏  韦宁  徐建哲  孙海波  董乐  刘东源  赵胜利  赵晓兰  孟庆顺  兰锋镝  黄显刚  李源哲  方林  李昌锋  李大为  孙永巧  张振鹏  杨峥  黄艳清  奚艳  亓秋辉  贺丹丹  黄海燕  张晓伟  王智强  李文杰  曹玉竹  余迎朴  俞鸿  李金宏  叶杰华  陈欢  周雁  刘斌  王晶  叶葭  纪海  李胜霆  倪培相  张建国  张勇  郑洪坤  毛炳宇  王文  叶辰  李松岗  汪建  杨焕明 《蚕学通讯》2008,28(4):1-16

我们在此报告了家蚕（Bombyxmori）的基因组序列框架图,它覆盖了所有已知家蚕基因的90．9％。我们估计的基因数是18510,超过黑腹果蝇报道的13379个基因。我们将家蚕基因组与果蝇、蚊子、蜘蛛和蝴蝶等进行了比较分析,揭示了它们在基因组成上同时具有相似性和差异性。  相似文献   

Purification and localization of nitric oxide synthases from hybrid tilapia (Nile tilapia x Mozambique tilapia)     

Wang WS  Hung SW  Lin YH  Tu CY  Wong ML  Chiou SH  Shieh MT 《Journal of aquatic animal health》2007,19(3):168-178

The aims of this study were to purify and localize the nitric oxide synthases (NOSs) from hybrid tilapia (Nile tilapia Oreochromis niloticus x Mozambique tilapia O. mossambicus). The purification procedures involved affinity chromatography with a 2', 5'-ADP-agarose 4B column and ion exchange with a diethylaminoethanol Bio-Gel A column. The results from gel filtration assays showed that the molecular weights of neuronal NOS (nNOS) and inducible NOS (iNOS) were 178 and 120 kDa, respectively. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis results showed that there were three bands with molecular weights of 89, 47, and 29 kDa from the purified nNOS. However, only one band, with a molecular weight of 120 kDa, appeared on the gel from the purified iNOS. Hybrid tilapia nNOS was a dimer structure, while iNOS appeared to be a monomer structure. Moreover, our results revealed that the activities of nNOS and iNOS were significantly higher after the addition of Ca+2 or Mg+2 ions individually. However, when L-arginine and NADPH were present, the addition of 1 mM of either ion did not further increase the activity. The chemical L-N(G)-methyl-L-arginine could inhibit the activities of the purified NOSs with or without L-arginine. Western blot analyses showed only an 89-kDa immunoreactive band from the extracts of cerebrum; however, we did not find the specific bands in other tissues, such as gill, intestine, liver, spleen, and anterior kidney. We found another 120-kDa immunoreactive protein band with the rabbit antirat iNOS serum against iNOS from the extracts of anterior kidney and spleen. The results of immunohistochemistry with the rabbit antihuman nNOS serum indicated that the nNOS existed in the cerebellum, olfactory bulb, diencephalons, and nerve cell bodies and neuronal fibers of the spinal cord. Interestingly, only macrophages from anterior kidney and spleen showed positive reactions with the rabbit antirat iNOS serum. In the same way, the endothelial NOS (eNOS) located in the heart and epithelial cells of the blood vessels reacted positively with the rabbit antibovine eNOS serum.  相似文献