Agrobacterium-mediated transformation of the CrDAT gene and selection of transgenic periwinkle lines with a high vincristine accumulation     

Thi Thanh Nhan Pham  Thi Ngoc Lan Nguyen  Thi Ha Bui  Huu Quan Nguyen  Thi Tam Nguyen  Van Son Le 《The Journal of Horticultural Science and Biotechnology》2019,94(5):591-598

Catharanthus roseus contains vincristine and vinblastine, which are outstanding drugs for cancer. In the biosynthetic pathways of terpenoid indole alkaloids (TIAs) in C. roseus, deacetylvindoline 4-O-acetyltransferase (DAT) is a key enzyme that catalyses the last reaction of vindoline biosynthesis to form vinblastine and vincristine. In this study, the CrDAT transgene was transferred into the periwinkle by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The C. roseus DAT gene was introduced into C. roseus plants and it was confirmed that CrDAT was successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein. Four transgenic periwinkle lines in T1 generation, T1-1, T1-3, T1-6, and T1-7, expressed recombinant DAT protein with the total protein content in the range of 2.86 μg.mg^?1 to 5.12 μg.mg^?1. Moreover, the vincristine contents of four transgenic lines increased by 1.63?2.48-fold compared to non-transgenic plants, ranging from 6.91 µg.g^?1 (fresh weight) to 10.53 µg.g^?1 (fresh weight). The T1-1 line had the highest vincristine content. Hence, the overexpression of the recombinant DAT protein can improve the vincristine accumulation of transgenic C. roseus plants.

Abbreviation: CrDAT - Catharanthus roseus Deacetylvindoline-4-O-Acetyl Transferase; D4H - Deacetoxyvindoline 4-hydroxylase; ELISA - Enzyme-Linked Immunosorbent Assay Monoterpene indole alkaloid; T0, T1 - Generations of transgenic plants; TIAs - Terpenoid indole alkaloids; WT- The wild-type tobacco plants (non transgenic plant); 35S - Cauliflower mosaic virus 35S promoter  相似文献   

Development of enzymes for robust aryloxyphenoxypropionate and synthetic auxin herbicide tolerance traits in maize and soybean crops     

Clayton T Larue  Michael Goley  Lei Shi  Artem G Evdokimov  Oscar C Sparks  Christine Ellis  Andrew M Wollacott  Timothy J Rydel  Coralie E Halls  Brook Van Scoyoc  Xiaoran Fu  Jeffrey R Nageotte  Adewale M Adio  Meiying Zheng  Eric J Sturman  Graeme S Garvey  Marguerite J Varagona 《Pest management science》2019,75(8):2086-2094

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Comparative energy and economic analyses of conventional and System of Rice Intensification (SRI) methods of rice production in Thai Nguyen Province,Vietnam     

Tuyet Thi Anh Truong  John Fry  Phu Van Hoang  Hoang Huy Ha 《Paddy and Water Environment》2017,15(4):931-941

The consumption of energy inputs in agricultural production has been increasing rapidly during the past decades. However, given the limitations and costs of non-renewable energy, increasing production while using the least energy possible has become a major concern of most nations. Prompted by this concern, we conducted a face-to-face survey of 90 farming households in Thai Nguyen Province, Vietnam, to find out how energy is being used in agriculture and, specifically, in their rice production. Through analysis of energy input–output balances, combined with economic efficiency analysis, a comparison was made of conventional and SRI methods of rice production. The study found that applying the SRI method can save around 23% of energy inputs, while increasing energy outputs by 11%. Economic benefits per hectare also rise by more than 8 million dong (USD 364) compared to those under the conventional cultivation system. The study also showed conflicts between the energy and economic balances for manual compared with machine ploughing operations. This study contributes to providing an overview of energy consumption in rice cultivation at the household level. Its findings can help stakeholders to assess current policies and make better decisions on the uses of energy in agricultural production. In addition, the comprehensive approach taken here to analysing energy use and efficiency could expand the analysis and comparison of energy uses at sectoral or activity level—still a new field in Vietnam and many other countries.  相似文献   

Induced expression of selected plant defence related genes in pot azalea, <Emphasis Type="Italic">Rhododendron simsii</Emphasis> hybrid     

Gil Luypaert  Johan Witters  Johan Van Huylenbroeck  Patrick De Clercq  Jan De Riek  Ellen De Keyser 《Euphytica》2017,213(10):227

A set of putative marker genes to study plant defense responses against Polyphagotarsonemus latus, a key pest in the production of Rhododendron simsii hybrids, was selected and validated. Genes belonged to the biosynthetic pathway of phytohormones jasmonic acid (JA) (RsLOX, RsAOS, RsAOC, RsOPR3 and RsJMT) and salicylic acid (SA) (RsPAL and RsICS). Furthermore, RsPPO, a putative marker gene for oxidative stress response was successfully cloned from R. simsii. A CTAB-based extraction protocol was optimized to assure excellent RNA quality for subsequent RT-qPCR analysis. The RT-qPCR protocol was extensively tested and RsRG7 and RsRG14 were selected as reference genes from a geNorm pilot study. Validation of the marker genes was done after application with elicitors [methyl jasmonate (MeJA), coronatine, β-aminobutyric acid and acibenzolar-Smethyl] or wounding. Both 100 μM MeJA and 0.1 μM coronatine had a significant effect on the expression of all marker genes. Foliar application of MeJA on the shoots resulted in a significantly earlier response when compared to root application and subsequent sampling of the shoots. Expression patterns after MeJA treatment were generally the same in six R. simsii genotypes: ‘Nordlicht’, ‘Elien’, ‘Aiko Pink’, ‘Michelle Marie’, ‘Mevrouw Gerard Kint’ and ‘Sachsenstern’. Wounding resulted in the same expression patterns as MeJA treatment except for RsJMT. None of the genotypes showed a significant induction of the latter gene 6 h upon wounding. Findings of these experiments indicated that the tolerant genotype ‘Elien’ has low basal expression levels of RsPPO. This might be the first step towards the breeding of mite-tolerant genotypes.  相似文献   

A review of carbon isotopes of phytoliths: implications for phytolith-occluded carbon sources     

Yang  Shilei  Hao  Qian  Wang  Hailong  Van Zwieten  Lukas  Yu  Changxun  Liu  Taoze  Yang  Xiaomin  Zhang  Xiaodong  Song  Zhaoliang 《Journal of Soils and Sediments》2020,20(4):1811-1823

Journal of Soils and Sediments - Phytolith-occluded carbon (PhytOC) is mainly derived from the products of photosynthesis, which can be preserved in soils and sediments for hundreds-to-thousands of...  相似文献   

Effects of porcine relaxin on induced parturition in beef heifers     

D.E. Smith  D.L. Hixon  D.W. Moore  E.A. Van Kirk  B.M. Alexander  R.V. Anthony  G.E. Moss 《Domestic animal endocrinology》1996,13(6):469-476

Two-year-old crossbred beef heifers were used to test the effects of porcine relaxin (pRelaxin) alone, or in combination with dexamethasone, on the induction of parturition, the incidence of dystocia, and retained placentas. Effects of treatment on pelvic area, postpartum interval, milk production, colostrum quality, calf birth weight, calf vigor, and calf performance were also evaluated. On Day 275 of gestation, heifers from two fetal-sire groups were randomly assigned to one of four groups in a 2 × 2 factorial design and received: no treatment (controls, N = 19), 20 mg of dexamethasone intramuscularly (im) (n = 22), 5 mg of pRelaxin (3,000 U/mg) im (n = 19), or 20 mg of dexamethasone plus 5 mg of pRelaxin (n = 17). Length of gestation (in days) was less (P < 0.05) in heifers treated with dexamethasone (279.8 ± 1.0) than in controls (286.6 ± 0.9), but was not influenced (P > 0.05) by treatment with pRelaxin. The incidence of retained placentas in heifers treated only with dexamethasone (27.3%) was not reduced by concomitant treatment with pRelaxin (35.3%). Retained placentas were not observed in any control heifers and in only one heifer (5.2%) treated solely with pRelaxin. Ease of calving (1 = unassisted, 5 = abnormal presentation) was not influenced by treatment (P > 0.05), even though birth weights (in kilograms) of calves from heifers treated with dexamethasone (36.4 ± 0.8) were less (P < .01) than those of calves from nondexamethasone-treated heifers (39.2 ± 0.8). Dexamethasone tended to reduce (P < 0.07) calf vigor (1 = healthy and strong, 5 = dead on arrival; 1.48 ± 0.11 vs. 1.18 ± 0.11), but was not (P > 0.05) influenced by pRelaxin. The duration of the postpartum anestrous interval (73.1 ± 1.8 d across groups) and pelvic areas following treatment and parturition were not influenced (P > 0.05) by dexamethasone or pRelaxin. Although determinants of colostrum quality (P < 0.01) and quantity (P < 0.08) of milk produced were influenced by dexamethasone, adjusted 205-d weights of calves did not differ (P > 0.05) among groups. In conclusion, treatment with pRelaxin alone failed to induce parturition or, when combined with dexamethasone, to reduce the incidence of retained placentas.  相似文献   

Multi-criteria decision making methods to address rural land allocation problems: A systematic review     

Sintayehu Legesse Gebre  Dirk Cattrysse  Esayas Alemayehu  Jos Van Orshoven 《国际水土保持研究(英文)》2021,9(4):490-501

Land allocation has been an important issue in land use planning research studies. Land allocation involves different multifunctional activities of maximizing environmental, economic and social benefits. Multi-criteria decision making (MCDM) method is the most popular tool to optimize land allocation problems by considering decision variables, conflicting objectives, and criteria. Hence, decision-makers face problems on how to optimize the land allocation while minimizing the conflicting trade-offs existing in the decision analysis. With this review study, we aim at identifying and extracting information on MCDM methods to solve land allocation problems from English language articles published between 2000 and 2019 and indexed by four scientific literature databases (Web of Science, Science Direct, Scopus, Google scholar). To this end, we applied a systematic literature review approach, i.e. the Preferred Reporting Items for Systematic Reviews and Meta-Analysis procedure (Moher et al., 2009), with a structured database search expression. 120 articles were selected of which, after careful screening of title, keywords and abstract, 69 were retained for detailed review. This review study report compiles comprehensive information by classifying the papers into application area, optimization objectives, criteria used, decision techniques, publication year and study region. In summary, we found that in the last two decades, the use of the MCDM method has increased, particularly in Europe and China. AHP (analytical hierarchal process) is frequently used for multi-attribute land allocation problems with reference to ecotourism and ecosystem management. LP (linear programming) and SA (simulating annealing) methods are predominantly used to optimize multi-objectives complex agricultural and forest land allocation problems respectively.  相似文献   

基于ANUSPLIN的时间序列气象要素空间插值   总被引：6，自引：0，他引：6  

刘志红  Tim R.McVicar  Li LingTao 《西北农林科技大学学报(自然科学版)》2008,36(10):227-234

【目的】介绍ANUSPLIN在黄土高原多沙粗沙区时间序列气象要素的空间插值过程,为相关人员在ANUSPLIN的参数设置、误差分析和协变量要素选择等方面提供参考。【方法】以薄盘样条函数为插值理论,以专用气象数据插值软件ANUSPLIN为实现工具,并引入一个或多个协变量线性子模型,来实现多个气象要素的空间插值。【结果】完成了黄土高原多沙粗沙区时间序列(1980~2000年)多个气象要素月平均数据的栅格化,计算了气象要素随其影响因子变化的关系。【结论】ANUSPLIN以薄盘光滑样条函数为理论基础,引入协变量线性子模型,能较好地提高气象要素空间插值精度,且能反映气象要素随其影响因子变化的比率关系。在大多数情况下,该区模型选择以样条次数为3次的局部薄盘光滑样条函数模型为最佳。温度的空间插值相对比较容易,且误差较小,1995-07平均相对误差为1%;风速、水汽压的误差中等;日照时数和降雨量的误差较大,个别情况相对误差可超过50%。  相似文献   

甜瓜属野生种Cucumis hystrix Chakr.胞质葡萄糖-6-磷酸脱氢酶基因的克隆与启动子结构分析     

曹明明  吴志明  娄群峰  阮氏清云  陈劲枫 《南京农业大学学报》2013,(2):25-30

根据植物葡萄糖-6-磷酸脱氢酶(G6PDH,EC1.1.1.49)氨基酸保守区域,设计简并引物,采用RT-PCR技术克隆得到甜瓜属野生种Cucumis hystrix Chakr.(2n=24)G6PDH基因cDNA片段;随后基于该序列设计特异引物,PCR方法筛选野生种BAC文库,获得2个BAC阳性单克隆。测序后获得了G6PDH基因全序列及其上游启动子序列,GenBank登录号:JQ771576。序列分析显示:G6PDH基因全长约6.5 kb,由15个外显子和14个内含子组成;内含子序列均符合5’-gt-ag-3’结构。外显子拼接后获得了G6PDH基因的开放阅读框(ORF)序列,序列全长1 551 bp,编码516个氨基酸,与黄瓜基因组网站公布的G6PDH基因核苷酸序列和氨基酸序列同源性分别为98.71%和99.03%。野生种G6PDH氨基酸序列N端缺少转运肽序列,确定为野生种胞质G6PDH。氨基酸序列与烟草、马铃薯、荷兰芹、猕猴桃、拟南芥、大豆、小麦、玉米、葡萄、杨树等植物胞质G6PDH同源性高达77%以上。系统发育树分析发现,甜瓜属胞质G6PDH与茄科植物最先聚类,植物胞质G6PDH在分子进化水平上与物种进化相符。启动子结构分析表明:序列含有启动子基本元件TATA-box、CAAT-box,还含有丰富的光响应元件,与环境胁迫响应相关的不同顺式作用元件,促进高水平转录的5UTR Py-rich stretch元件和提高表达的CAT-box元件等。  相似文献   

中国彭州市和越南东安地区蔬菜生产的肥料使用和评价     

林超文  陈一兵  Nguyen Van Dung  李浩 《西南农业学报》2008,21(2):412-419

本文详细阐述了中国和越南4个村蔬菜生产中的肥料施用情况,概述了施用肥料的种类、施用时间和施用数量。评价了影响肥料施用的因素,比较了施肥量和蔬菜从土壤中带出的养分量。在研究区域,农户施用了50多种肥料,施氮量间于0～700kg／hm^2。肥料施用时间因蔬菜种类和地区不同而不同。氮的施用量主要受作物种类影响。大部分中国蔬菜的氮、磷施用量过大,而钾肥施用量不足;相对而言,越南蔬菜的局部养分平衡比中国的情况好一些。因此,局部的养分平衡显示了不同蔬菜种类、不同季节和不周村社的养分投入量和养分输出量有很大区别。  相似文献