Agrobacterium-mediated transformation of the CrDAT gene and selection of transgenic periwinkle lines with a high vincristine accumulation     

Thi Thanh Nhan Pham  Thi Ngoc Lan Nguyen  Thi Ha Bui  Huu Quan Nguyen  Thi Tam Nguyen  Van Son Le 《The Journal of Horticultural Science and Biotechnology》2019,94(5):591-598

Catharanthus roseus contains vincristine and vinblastine, which are outstanding drugs for cancer. In the biosynthetic pathways of terpenoid indole alkaloids (TIAs) in C. roseus, deacetylvindoline 4-O-acetyltransferase (DAT) is a key enzyme that catalyses the last reaction of vindoline biosynthesis to form vinblastine and vincristine. In this study, the CrDAT transgene was transferred into the periwinkle by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The C. roseus DAT gene was introduced into C. roseus plants and it was confirmed that CrDAT was successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein. Four transgenic periwinkle lines in T1 generation, T1-1, T1-3, T1-6, and T1-7, expressed recombinant DAT protein with the total protein content in the range of 2.86 μg.mg^?1 to 5.12 μg.mg^?1. Moreover, the vincristine contents of four transgenic lines increased by 1.63?2.48-fold compared to non-transgenic plants, ranging from 6.91 µg.g^?1 (fresh weight) to 10.53 µg.g^?1 (fresh weight). The T1-1 line had the highest vincristine content. Hence, the overexpression of the recombinant DAT protein can improve the vincristine accumulation of transgenic C. roseus plants.

Abbreviation: CrDAT - Catharanthus roseus Deacetylvindoline-4-O-Acetyl Transferase; D4H - Deacetoxyvindoline 4-hydroxylase; ELISA - Enzyme-Linked Immunosorbent Assay Monoterpene indole alkaloid; T0, T1 - Generations of transgenic plants; TIAs - Terpenoid indole alkaloids; WT- The wild-type tobacco plants (non transgenic plant); 35S - Cauliflower mosaic virus 35S promoter  相似文献   

Neuroprotective Metabolites from Vietnamese Marine Derived Fungi of Aspergillus and Penicillium Genera     

Elena V. Girich  Anton N. Yurchenko  Olga F. Smetanina  Phan Thi Hoai Trinh  Ngo Thi Duy Ngoc  Mikhail V. Pivkin  Roman S. Popov  Evgeny A. Pislyagin  Ekaterina S. Menchinskaya  Ekaterina A. Chingizova  Shamil S. Afiyatullov  Ekaterina A. Yurchenko 《Marine drugs》2020,18(12)

Low molecular weight secondary metabolites of marine fungi Aspergillus flocculosus, Aspergillus terreus and Penicillium sp. from Van Phong and Nha Trang Bays (Vietnam) were studied and a number of polyketides, bis-indole quinones and terpenoids were isolated. The structures of the isolated compounds were determined by 1D and 2D NMR and HR-ESI-MS techniques. Stereochemistry of some compounds was established based on ECD data. A chemical structure of asterriquinone F (6) was thoroughly described for the first time. Anthraquinone (13) was firstly obtained from a natural source. Neuroprotective influences of the isolated compounds against 6-OHDA, paraquat and rotenone toxicity were investigated. 4-Hydroxyscytalone (1), 4-hydroxy-6-dehydroxyscytalone (2) and demethylcitreoviranol (3) have shown significant increasing of paraquat- and rotenone-treated Neuro-2a cell viability and anti-ROS activity.  相似文献   

Effects of elevated CO2 concentration on bulbil germination and early seedling growth in Chinese yam under different air temperatures     

Nguyen Cong Thinh  Etsushi Kumagai  Hiroyuki Shimono 《Plant Production Science》2017,20(3):313-322

The present study investigated the effects of elevated carbon dioxide concentration ([CO₂]) and air temperature on the germination of seed bulbils and the seedling vigour of two Chinese yam lines. Plants were grown under two [CO₂] levels, ambient and elevated (ambient + 200 μmol mol^?1), and two mean air temperature regimes, 22.2 °C (ambient + 1.4 °C) and 25.6 °C (ambient + 5.2 °C). Elevated [CO₂] did not affect bulbil germination under both air temperature regimes. During the early growth stage, the dry weight (DW) of leaves, vines, shoots, roots, belowground parts (roots + tubers) and whole plants were higher under elevated [CO₂] than ambient [CO₂] for both lines under the low- and high-temperature regimes. The values of vigour indexes (index I = germination % × seedling length and index II = germination % × seedling DW) were also higher under elevated [CO₂] than ambient [CO₂] for both lines. These results indicated that Chinese yam seedlings respond positively to elevated [CO₂] during the early growth stage. The above:belowground DW ratios were lower under elevated [CO₂] than ambient [CO₂] in seedlings with very small new tubers for both yam lines, indicating that elevated [CO₂] strongly affected the root growth in the early growth stage. The DWs of post-treatment seed bulbils were higher in the elevated [CO₂] under both air temperature regimes. The results showed that Chinese yam used a smaller amount of the reserves in seed bulbils under elevated [CO₂] than under ambient [CO₂].  相似文献   

Ouabain-sensitive Na/K-ATPase activity increases during embryogenesis in the giant freshwater prawn Macrobrachium rosenbergii     

Marcy N Wilder  Do Thi Thanh Huong  Atsuro Okuno  Muharijadi Atmomarsono  Wei-Jun Yang 《Fisheries Science》2001,67(1):182-184

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Na/K-ATPase activity during larval development in the giant freshwater prawn Macrobrachium rosenbergii and the effects of salinity on survival rates     

Do Thi  Thanh HUONG  Vidya  JAYASANKAR  Safiah  JASMANI  Hisako  SAIDO-SAKANAKA  Andrew J.  WIGGINTON  Marcy N.  WILDER 《Fisheries Science》2004,70(3):518-520

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A method for monitoringBemisia tabaci and timing spray applications against the pest in cotton fields in israel     

Venezia Melamed-Madjar  S. Cohen  M. Chen  S. Tam  Denise Rosilio 《Phytoparasitica》1982,10(2):85-91

A method for monitoring tobacco whitefly [Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae)] populations was developed and used for timing spray applications against this pest in cotton. It was found to be successful in tests in eight commercial cotton fields. A trap composed of a yellow plastic plate (15 × 20 cm) smeared on the upper side with a thin layer of glue, was attached to an iron pole which held the plate in a horizontal position above the plants. The traps were placed 400 m apart along the periphery of the field. The sticky plates were changed weekly and the adult whiteflies were counted. When the mean weekly catch per trap reached ~ 100–200 adults, spray applications were carried out. Following this treatment,B. tabaci populations remained low and sooty mould did not develop on the lint.  相似文献   

Effects of Dietary Supplementation of Barodon on Growth Performance,Innate Immunity and Disease Resistance of Juvenile Olive Flounder,Paralichthys olivaceus,Against Streptococcus iniae     

Chang‐Hoon Shin  Hien Thi Dieu Bui  Samad Rahimnejad  Ji‐Hoon Cha  Byung‐Woo Yoo  Bo‐Kyeun Lee  Hyung‐Jin Ahn  Soo‐Il Choi  Yun‐Jeong Choi  Yong‐Ho Park  Jeong‐Dae Kim  Kyeong‐Jun Lee 《Journal of the World Aquaculture Society》2014,45(3):258-268

This study was conducted to evaluate the effects of dietary supplementation of Barodon, an anionic alkali mineral complex, on growth, feed utilization, humoral innate immunity and disease resistance of olive flounder. A basal experimental diet was used as a control and supplemented with 0.1, 0.2, 0.3, 0.4, or 0.5% Barodon. Triplicate groups of fish (26.4 ± 0.2 g) were fed one of the diets to apparent satiation twice daily for 10 wk. The growth performance was enhanced (P < 0.05) linearly and quadratically in fish fed diets containing Barodon compared with that in fish fed the control. Feed utilization was significantly improved by Barodon supplementation. Serum lysozyme and antiprotease activities were increased quadratically in Barodon fed groups. Also, significantly higher superoxide dismutase activity was found in Barodon‐fed fish. Dietary supplementation of 0.1–0.3% Barodon resulted in significant enhancement of fish disease resistance against Streptococcus iniae. The findings in this study indicate that dietary supplementation of Barodon can enhance growth, feed utilization, innate immunity, and disease resistance of olive flounder and that the optimum level seems to be 0.1% in diets.  相似文献   

Effects of Fermentation Conditions Using Lactobacillus plantarum on the Charantin,Stigmasterol Glucoside and β-sitosterol Glucoside Contents of Bitter Gourd (Momordica charantia L.) Juice     

Nguyen  Trang T. T.  Nguyen  Ha V. H. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(4):656-658

Plant Foods for Human Nutrition - Bitter gourd fruits contain high amounts of charantin, stigmasterol glucoside and β-sitosterol glucoside, which have been shown to provide health benefits for...  相似文献   

Differences in Ion Accumulation and Salt Tolerance among Glycine Accessions     

J. M. Lenis  M. Ellersieck  D. G. Blevins  D. A. Sleper  H. T. Nguyen  D. Dunn  J. D. Lee  J. G. Shannon 《Journal of Agronomy and Crop Science》2011,197(4):302-310

Salinity reduces crop yield by limiting water uptake and causing ion‐specific stress. Soybean [Glycine max (L.) Merr.] is sensitive to soil salinity. However, there is variability among soybean genotypes and wild relatives for salt tolerance, suggesting that genetic improvement may be possible. The objective of this study was to identify differences in salt tolerance based on ion accumulation in leaves, stems and roots among accessions of four Glycine species. Four NaCl treatments, 0, 50, 75 and 100 mm , were imposed on G. max, G. soja, G. tomentella and G. argyrea accessions with different levels of salinity tolerance. Tolerant genotypes had less leaf scorch and a greater capacity to prevent Na⁺ and Cl^? transport from soil solution to stems and leaves than sensitive genotypes. Magnitude of leaf injury per unit increase in leaf Na⁺ or Cl^? concentrations was lower in tolerant than in susceptible accessions. Also, plant injury was associated more with Na⁺ rather than with Cl^? concentration in leaves. Salt‐tolerant accessions had greater leaf chlorophyll‐meter readings than sensitive genotypes at all NaCl concentrations. Glycine argyrea and G. tomentella accessions possessed higher salt tolerance than G. soja and G. max genotypes.  相似文献   

The optimal nutrition of maize in the hungarian national long‐term field experimental network     

Tamás Kismányoky  Katalin Debreczeni 《Archives of Agronomy and Soil Science》2013,59(3-4):251-264

In Hungary, maize is grown on 1 million ha and occupies more than 20% of the arable land. The rich assortment of maize cultivars of different vegetation periods and different responses to nutritional effects, water supply etc. gives the growers the possibility to choose the cultivars suiting best the site characteristics (Jolânkai et al. 1999). Among the cereals maize has the highest genetical potential. To utilize its yield and quality potential, soil types of high nutrient content and regular nutrient supply are required (Gyõrffy, 1979). Both over‐ and under‐fertilization have an unfavourable effect on the yield and quality of maize (Debreczeni, 1985). Crops can be supplied with the appropriate nutrient amounts only with the knowledge of soil characteristics in the different agro‐ecological regions (nutrient content, water supply, soil compactness, pH, nutrient supplying capacity etc.). In Hungary, a network of long‐term field fertilization trials with uniform fertilizer treatments has been maintained at nine experimental sites representing different agro‐ecological regions of the country. This experimental network gives a basis to test the nutrient responses of our main crops and calibrate their optimal nutrient supply (Kismányoky, 1991).  相似文献