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Thi Thanh Nhan Pham Thi Ngoc Lan Nguyen Thi Ha Bui Huu Quan Nguyen Thi Tam Nguyen Van Son Le 《The Journal of Horticultural Science and Biotechnology》2019,94(5):591-598
Catharanthus roseus contains vincristine and vinblastine, which are outstanding drugs for cancer. In the biosynthetic pathways of terpenoid indole alkaloids (TIAs) in C. roseus, deacetylvindoline 4-O-acetyltransferase (DAT) is a key enzyme that catalyses the last reaction of vindoline biosynthesis to form vinblastine and vincristine. In this study, the CrDAT transgene was transferred into the periwinkle by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The C. roseus DAT gene was introduced into C. roseus plants and it was confirmed that CrDAT was successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein. Four transgenic periwinkle lines in T1 generation, T1-1, T1-3, T1-6, and T1-7, expressed recombinant DAT protein with the total protein content in the range of 2.86 μg.mg?1 to 5.12 μg.mg?1. Moreover, the vincristine contents of four transgenic lines increased by 1.63?2.48-fold compared to non-transgenic plants, ranging from 6.91 µg.g?1 (fresh weight) to 10.53 µg.g?1 (fresh weight). The T1-1 line had the highest vincristine content. Hence, the overexpression of the recombinant DAT protein can improve the vincristine accumulation of transgenic C. roseus plants.Abbreviation: CrDAT - Catharanthus roseus Deacetylvindoline-4-O-Acetyl Transferase; D4H - Deacetoxyvindoline 4-hydroxylase; ELISA - Enzyme-Linked Immunosorbent Assay Monoterpene indole alkaloid; T0, T1 - Generations of transgenic plants; TIAs - Terpenoid indole alkaloids; WT- The wild-type tobacco plants (non transgenic plant); 35S - Cauliflower mosaic virus 35S promoter 相似文献
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Elena V. Girich Anton N. Yurchenko Olga F. Smetanina Phan Thi Hoai Trinh Ngo Thi Duy Ngoc Mikhail V. Pivkin Roman S. Popov Evgeny A. Pislyagin Ekaterina S. Menchinskaya Ekaterina A. Chingizova Shamil S. Afiyatullov Ekaterina A. Yurchenko 《Marine drugs》2020,18(12)
Low molecular weight secondary metabolites of marine fungi Aspergillus flocculosus, Aspergillus terreus and Penicillium sp. from Van Phong and Nha Trang Bays (Vietnam) were studied and a number of polyketides, bis-indole quinones and terpenoids were isolated. The structures of the isolated compounds were determined by 1D and 2D NMR and HR-ESI-MS techniques. Stereochemistry of some compounds was established based on ECD data. A chemical structure of asterriquinone F (6) was thoroughly described for the first time. Anthraquinone (13) was firstly obtained from a natural source. Neuroprotective influences of the isolated compounds against 6-OHDA, paraquat and rotenone toxicity were investigated. 4-Hydroxyscytalone (1), 4-hydroxy-6-dehydroxyscytalone (2) and demethylcitreoviranol (3) have shown significant increasing of paraquat- and rotenone-treated Neuro-2a cell viability and anti-ROS activity. 相似文献
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The present study investigated the effects of elevated carbon dioxide concentration ([CO2]) and air temperature on the germination of seed bulbils and the seedling vigour of two Chinese yam lines. Plants were grown under two [CO2] levels, ambient and elevated (ambient + 200 μmol mol?1), and two mean air temperature regimes, 22.2 °C (ambient + 1.4 °C) and 25.6 °C (ambient + 5.2 °C). Elevated [CO2] did not affect bulbil germination under both air temperature regimes. During the early growth stage, the dry weight (DW) of leaves, vines, shoots, roots, belowground parts (roots + tubers) and whole plants were higher under elevated [CO2] than ambient [CO2] for both lines under the low- and high-temperature regimes. The values of vigour indexes (index I = germination % × seedling length and index II = germination % × seedling DW) were also higher under elevated [CO2] than ambient [CO2] for both lines. These results indicated that Chinese yam seedlings respond positively to elevated [CO2] during the early growth stage. The above:belowground DW ratios were lower under elevated [CO2] than ambient [CO2] in seedlings with very small new tubers for both yam lines, indicating that elevated [CO2] strongly affected the root growth in the early growth stage. The DWs of post-treatment seed bulbils were higher in the elevated [CO2] under both air temperature regimes. The results showed that Chinese yam used a smaller amount of the reserves in seed bulbils under elevated [CO2] than under ambient [CO2]. 相似文献
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感染白粉病小麦叶片细胞的超微变化 总被引:2,自引:0,他引:2
兰景华 《西南大学学报(自然科学版)》1992,14(4):289-291
小麦感染白粉病菌后,叶片叶绿体肿胀,双层膜不完整或消失,基粒片层排列疏松紊乱,细胞中出现许多由单层膜围成的大小不等的空胞和类似纤维状的膜状物,细胞核膜消失,质壁分离. 相似文献
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茉莉酸对苹果气孔运动及抗旱性的影响 总被引:5,自引:0,他引:5
50、75、100、150 mg/L JA处理苹果叶片表皮,使开放气孔的气孔开度分别减小27.4%、37.5%、56.9%和63.8%,使关闭后的气孔在光下开放时气孔开度分别减小15%、18%、28.8%、52.5%、61.3%;处理植株后干旱胁迫,叶片相对电导率分别降低15%、18%、31%、58%,脯氨酸含量分别提高0.09%、0.40%、0.42%、0.54 ng/gFW,可溶性糖含量分别提高1.89%、2.53%、3.80%、5.06%。25 mg/L JA处理与对照均无显著差别。JA的作用表现出明显的浓度效应。 相似文献
9.
采用显示性竞争优势指数法(CompetitiveAdvantage,CA),结合翔实的数据,对中国丝绸产业的国际竞争力进行较为全面的实证分析,分析结果表明中国的丝绸国际竞争力呈直线下降的趋势。笔者认为影响中国丝绸产业国际竞争力的主要因素为:(1)产品质量与世界先进水平仍然存在着较大的差距;(2)中国的茧丝绸管理体制不适应市场经济的要求,严重的阻碍了中国丝绸国际竞争力的发展;(3)行业法规迟迟不能出台,宏观调控能力乏力;(4)中国的品牌效应在中国仍然没有真正形成;等。笔者还进一步就提高中国丝绸产业的国际竞争力的提出自己的建议:(1)改革和理顺宏观管理体制;(2)加快茧丝绸行业法规制定,加强行业管理的权威性,加大宏观调控的力度;(3)加大中国的科技投入,争取实现各项关键技术的重大突破;(4)进一步巩固“东桑西移”产业布局调整的成果,加快促进产业升级和技术进步;(5)加大对丝绸产品的宣传力度,尽快开发中国的丝绸市场;(6)进一步宣传和实施中国的高档丝绸标志的品牌战略,提高中国丝绸品牌的知名度。 相似文献
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提出了一种简单的电流模式多功能滤波器结构,具有一个输入端和三个输出端,可以实现低通,高通,带通,全通和陷波功能,不需要任何严格匹配关系,该结构中使用了6个CC器件,两个接地电阻和两个接地电容,结构十分简单且无源灵敏度很低。 相似文献