Agrobacterium-mediated transformation of the CrDAT gene and selection of transgenic periwinkle lines with a high vincristine accumulation     

Thi Thanh Nhan Pham  Thi Ngoc Lan Nguyen  Thi Ha Bui  Huu Quan Nguyen  Thi Tam Nguyen  Van Son Le 《The Journal of Horticultural Science and Biotechnology》2019,94(5):591-598

Catharanthus roseus contains vincristine and vinblastine, which are outstanding drugs for cancer. In the biosynthetic pathways of terpenoid indole alkaloids (TIAs) in C. roseus, deacetylvindoline 4-O-acetyltransferase (DAT) is a key enzyme that catalyses the last reaction of vindoline biosynthesis to form vinblastine and vincristine. In this study, the CrDAT transgene was transferred into the periwinkle by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The C. roseus DAT gene was introduced into C. roseus plants and it was confirmed that CrDAT was successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein. Four transgenic periwinkle lines in T1 generation, T1-1, T1-3, T1-6, and T1-7, expressed recombinant DAT protein with the total protein content in the range of 2.86 μg.mg^?1 to 5.12 μg.mg^?1. Moreover, the vincristine contents of four transgenic lines increased by 1.63?2.48-fold compared to non-transgenic plants, ranging from 6.91 µg.g^?1 (fresh weight) to 10.53 µg.g^?1 (fresh weight). The T1-1 line had the highest vincristine content. Hence, the overexpression of the recombinant DAT protein can improve the vincristine accumulation of transgenic C. roseus plants.

Abbreviation: CrDAT - Catharanthus roseus Deacetylvindoline-4-O-Acetyl Transferase; D4H - Deacetoxyvindoline 4-hydroxylase; ELISA - Enzyme-Linked Immunosorbent Assay Monoterpene indole alkaloid; T0, T1 - Generations of transgenic plants; TIAs - Terpenoid indole alkaloids; WT- The wild-type tobacco plants (non transgenic plant); 35S - Cauliflower mosaic virus 35S promoter  相似文献   

Effect of carbon/nitrogen ratio manipulation in feed supplements on Artemia production and water quality in solar salt ponds in the Mekong Delta,Vietnam     

Lulijwa Ronald  Gilbert Van Stappen  Nguyen Van Hoa  Patrick Sorgeloos 《Aquaculture Research》2014,45(12):1906-1912

The effect of carbon/nitrogen (C/N) ratio manipulation in feed supplements on Artemia production and water quality was investigated in solar salt ponds in the Mekong Delta, Vietnam. It was assumed that development of bacterial bioflocs through C/N manipulation would improve Artemia production and water quality as demonstrated in freshwater and marine aquaculture. Twelve ponds were used for three treatments and the control, in triplicates. Green water (GW) was supplied to all ponds, with the standard Vietnamese procedure of supplying GW and chicken manure (CM) as the control (C/N 1.8). Treatment ponds were supplemented with tapioca (TAP) as carbon source, combined with either CM, pig manure (PM) or rice bran (RB), with C/N ratios of 7.4, 10.5 or 20.1 respectively. After 6 weeks of culture, no single treatment supported both improved water quality and enhanced Artemia production. Overall, improved water quality was observed at C/N 20.1 and higher Artemia production at C/N 7.4. Although external field factors could have interfered with the set‐up, this is the first study on the effect of C/N manipulation in feed supplements in Artemia pond production systems and provides the basis for development of bacterial bioflocs as a technology to improve water quality and Artemia production.  相似文献   

Can fermented soybean meal and squid by‐product blend be used as fishmeal replacements for Japanese flounder (Paralichthys olivaceus)?     

Md. Abdul Kader  Shunsuke Koshio  Manabu Ishikawa  Saichiro Yokoyama  Mahbuba Bulbul  Binh Thanh Nguyen  Jian Gao  Asda Laining 《Aquaculture Research》2012,43(10):1427-1438

An experiment was carried out to evaluate fermented soybean meal and squid by‐product blend (1:1) (FP) as replacement of fishmeal (FM) for Japanese flounder, Paralichthys olivaceus. Five isocaloric (19 kJ g^?1) diets were prepared by replacing 0 (FP0), 12 (FP12), 24 (FP24), 36 (FP36) and 48% (FP48) FM protein with FP. Triplicate groups of juveniles (mean weight of 3.9 g) were delivered the test diets for 8 weeks in a flow‐through sea water system. The results showed that there were no significant differences (P > 0.05) among the growth rates of fish fed FP0, FP12, FP24 and FP36 diets. Growth and nutrient utilization parameters were significantly reduced in fish fed FP48 diet. Although, whole body proximate composition of fish were not significantly affected by the dietary treatments compared to the control; methionine and phenylalanine contents were significantly decreased in FP48 group. Protein retention was also significantly decreased in the similar group of fish. Dietary treatments did not alter most of the plasma metabolites, while some of the health parameters were improved in the replacement groups. Results suggested that FP is a potential candidate for alternative protein ingredient in aquafeed and can replace 36% FM protein in the diet of Japanese flounder.  相似文献   

Effects of Dietary Digestible Protein and Energy Levels on Growth Performance,Feed Utilization,and Body Composition of Juvenile Permit,Trachinotus falcatus (Linnaeus, 1758)          下载免费PDF全文

Huy Quang Nguyen  Thiet Chi Chu  Thuy Thi Le Nguyen  Ivar Lund 《Journal of the World Aquaculture Society》2018,49(5):943-952

A 9‐wk study was conducted to evaluate the effect of dietary protein and energy on growth performance of juvenile permit, Trachinotus falcatus, growing from approximately 30 to 150 g. Nine experimental diets were formulated to contain three levels of crude protein (400, 450, and 500 g/kg dry matter [DM]); and three levels of crude lipid (100, 200, and 300 g/kg DM) in a 3 × 3 factorial design. Growth rate and feed efficiency were significantly improved with increasing dietary protein levels from 400 to 500 g/kg and with dietary lipid levels from 100 to 200 g/kg. Fish body protein content was positively correlated with dietary ratio of digestible protein (DP) to digestible energy (DE) (P < 0.01, R² = 0.83), while body lipid was negatively correlated with dietary DP/DE (R² = 0.55, P < 0.05) but positively correlated with dietary DE levels (R² = 0.66, P < 0.01). Results showed a protein‐sparing effect, as protein retention was significantly increased by increasing dietary lipid level. In conclusion, the diet containing DP of 392.7 g/kg and DE of 18.8 MJ/kg (DM), corresponding to a DP/DE of 20.9 g/MJ, is suggested as an optimal feed for growth and feed efficiency in juvenile permit.  相似文献   

Effects of elevated CO2 concentration on bulbil germination and early seedling growth in Chinese yam under different air temperatures     

Nguyen Cong Thinh  Etsushi Kumagai  Hiroyuki Shimono 《Plant Production Science》2017,20(3):313-322

The present study investigated the effects of elevated carbon dioxide concentration ([CO₂]) and air temperature on the germination of seed bulbils and the seedling vigour of two Chinese yam lines. Plants were grown under two [CO₂] levels, ambient and elevated (ambient + 200 μmol mol^?1), and two mean air temperature regimes, 22.2 °C (ambient + 1.4 °C) and 25.6 °C (ambient + 5.2 °C). Elevated [CO₂] did not affect bulbil germination under both air temperature regimes. During the early growth stage, the dry weight (DW) of leaves, vines, shoots, roots, belowground parts (roots + tubers) and whole plants were higher under elevated [CO₂] than ambient [CO₂] for both lines under the low- and high-temperature regimes. The values of vigour indexes (index I = germination % × seedling length and index II = germination % × seedling DW) were also higher under elevated [CO₂] than ambient [CO₂] for both lines. These results indicated that Chinese yam seedlings respond positively to elevated [CO₂] during the early growth stage. The above:belowground DW ratios were lower under elevated [CO₂] than ambient [CO₂] in seedlings with very small new tubers for both yam lines, indicating that elevated [CO₂] strongly affected the root growth in the early growth stage. The DWs of post-treatment seed bulbils were higher in the elevated [CO₂] under both air temperature regimes. The results showed that Chinese yam used a smaller amount of the reserves in seed bulbils under elevated [CO₂] than under ambient [CO₂].  相似文献   

Should only females of giant freshwater prawn Macrobrachium rosenbergii be selected in genetic improvement programmes?     

Nguyen Thanh Vu  Tran Huu Phuc  Nguyen Trung Ky  Nguyen Thi Kieu Nga  Nguyen Hong Nguyen 《Aquaculture Research》2020,51(4):1381-1387

Sexual dimorphism is widely observed in almost all farmed aquatic animal species but giant freshwater pawn (GFP) is unique, with males characterized by three main morphotypes (blue claw, orange claw and small males) and females by different reproduction status (ovary, berried egg and already‐spawned females). There has been reported evidence that the effect of male morphotype may have masked genetic variation in growth‐related traits, as a result the heritability for male body weight was lower than that estimated in female. A pending question has arisen whether selection should be made in female only. To answer this question, we used an 8‐year data set from a long‐term selection programme (2008–2015) for high growth in this species comprising 106,756 individuals that were offspring of 515 sires and 810 dams. The body weight data of female and male GFP or of each morphotype was treated as a separate trait and a multi‐trait approach was used to estimate genetic correlations for homologous traits between sexes and between morphotypes. Our analysis showed that there were little differences in the heritability estimates between female and male. In female, mature ovary individual displayed higher heritability than berried egg and already‐spawned females. For male, the heritability for blue claw, orange claw and small males were 0.11, 0.06 and 0.00 respectively. Between‐sex genetic correlation was moderate (0.55 ± 0.11) for body weight, suggesting that the trait expressions in female and male may be genetically different. In female, the genetic correlations for body weight among three female types were close to one (0.91–0.94). In contrast, the genetic correlations for body weight between male morphotypes especially between blue claw or orange claw and small males were low (0.15–0.25). Furthermore, we estimated genetic gain as the difference in least square means (LSM) or estimated breeding values (EBV) between the selection line and control group. The genetic gain in body weight was smaller in females than in males. It is concluded that there is no need to run separate breeding programme for female and male GFP. A combined selection using both female and male data can achieve selection response for body weight as demonstrated in the present study.  相似文献   

Responses in residual feed intake in lines of Large White pigs selected for growth rate on restricted feeding (measured on ad libitum individual feeding)     

N.H. Nguyen  C.P. McPhee  & C.M. Wade 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2005,122(4):264-270

Residual feed intake (RFI) represents the deviation of the actual ad libitum food consumption of each animal from that predicted from combination of growth rate, backfat and metabolic body weight measurements. After 4 years of divergent selection of pigs in lines for either high or low postweaning growth rate on restricted feeding, the high line exhibited a significant reduction in RFI relative to the low line. This indicated, to some extents, a lower energy requirement for maintenance in the high than in the low line, possibly because of reduced physical activity of the animals. Estimates of genetical parameters showed that RFI was moderately heritable and those genetic correlations of RFI with carcass backfat and food conversion ratio were moderate to highly positive.  相似文献   

The epidemiology of nematode and fluke infections in cattle in the Red River Delta in Vietnam   总被引：1，自引：0，他引：1  

Holland WG  Luong TT  Nguyen LA  Do TT  Vercruysse J 《Veterinary parasitology》2000,93(2):141-147

Over a period of 13 months, faecal samples were collected monthly from approximately 45 cattle over 3 months of age. Additionally, 74 calves of 1-2 months were sampled to determine the presence of Toxocara vitulorum eggs. Individual egg counts and infective strongyle larvae from pooled faecal samples were examined. Post-mortem worm counts were carried out on six groups of tracer calves (n=12) that had been kept for 4 weeks on pasture in and around the village studied. The following helminths were identified: T. vitulorum, Cooperia punctata, C. pectinata, C. oncophora, Oesophagostomum radiatum, Trichostrongylus axei, T. colubriformis, Haemonchus spp., Fasciola spp. and Paramphistomum spp. In 8% of the samples collected from young calves, individual egg counts for T. vitulorum were found indicative for pathogenic worm burdens. Strongyle egg counts and worm counts indicated that transmission is low without a distinct seasonality. In animals of 3-9 months old, a strongyle egg count peak can be demonstrated which at a higher age steadily and significantly decreased. In faecal cultures Cooperia spp. were most prominent in all age groups throughout the year with the exception of the period September-November when Haemonchus spp. and Oesophagostomum spp. were most prevalent. Fasciola spp. eggs were found in 22% of the collected faecal samples and the egg counts were low indicating that the intensity of Fasciola spp. infection is mild. Based on the present data, regular anthelmintic treatments seem not to be justified, except for a single treatment at the age of 2 weeks against toxocariosis.  相似文献   

Passive protection of dogs against clinical disease due to Canine parvovirus-2 by specific antibody from chicken egg yolk   总被引：4，自引：0，他引：4       下载免费PDF全文

Sa Van Nguyen  Kouji Umeda  Hideaki Yokoyama  Yukinobu Tohya    Yoshikatsu Kodama 《Canadian journal of veterinary research》2006,70(1):62-64

The protective effect of immunoglobulins derived from chicken egg yolk (IgY) against infection by Canine parvovirus 2 (CPV-2) was evaluated in 10 beagle dogs orally challenged with a strain of the virus. The 2-mo-old dogs were divided into 3 groups and treated with powders containing CPV-2 IgY or normal egg yolk for 7 d after the challenge. The 4 dogs receiving normal egg yolk (control group) demonstrated mild symptoms typical of CPV-2 infection, such as vomiting, diarrhea, and weight loss. No symptoms were observed by 16 d after challenge in the 3 dogs receiving 2 g of IgY powder. Of the 3 dogs receiving 0.5 g of IgY powder, 2 had clinical CPV-2 disease; however, the manifestations were less severe than in the control group. Furthermore, the IgY-treated groups had significantly greater weight gain and shorter duration of virus shedding than the control group. These results indicate that IgY is useful in protecting dogs from CPV-2-induced clinical disease.  相似文献   

Responses in carcass lean pH at 24 hours post-mortem in lines of Large White pigs selected for growth rate on a fixed ration over a set time     

N.H. Nguyen  C.P. McPhee  G.R. Trout 《Livestock Science》2006,100(2-3):84-90

A restricted maximum likelihood analysis applied to an animal model showed no significant differences (P > 0.05) in pH value of the longissimus dorsi measured at 24 h post-mortem (pH24) between high and low lines of Large White pigs selected over 4 years for post-weaning growth rate on restricted feeding. Genetic and phenotypic correlations between pH24 and production and carcass traits were estimated using all performance testing records combined with the pH24 measurements (5.05–7.02) on slaughtered animals. The estimate of heritability for pH24 was moderate (0.29 ± 0.18). Genetic correlations between pH24 and production or carcass composition traits, except for ultrasonic backfat (UBF), were not significantly different from zero. UBF had a moderate, positive genetic correlation with pH24 (0.24 ± 0.33). These estimates of genetic correlations affirmed that selection for increased growth rate on restricted feeding is likely to result in limited changes in pH24 and pork quality since the selection does not put a high emphasis on reduced fatness.  相似文献