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91.
Godelieve Gheysen Walter Van der Eycken Nathalie Barthels Mansour Karimi Marc Van Montagu 《Pest management science》1996,47(1):95-101
The molecular interactions between plants and sedentary nematodes are undergoing intense study, not only for reasons of fundamental research but also for the potential benefits to agriculture. The present technology allows the transformation of an increasing number of crop plants, providing new ways to introduce resistance against plant-parasitic nematodes. The ability of sedentary nematodes to induce specialized feeding sites in plant roots is one of the most fascinating aspects of this host–parasite interaction. Molecular approaches have been initiated to identify and characterize plant genes altered in expression after infection by sedentary nematodes. The results obtained indicate that many genes indeed become up-regulated upon nematode infection. Surprisingly, several so-called constitutive promoters that are normally used to achieve high expression in plant cells are completely ‘silenced’ in the feeding sites within days after nematode infection. Generally, there are two options available for the genetic engineering of nematode resistance: the synthesis of anti-nematode proteins or the localized production of a cytotoxic protein that interferes with the development of feeding cells. Nematode-induced promoters are very useful for the production by plants of sufficiently high levels of anti-nematode proteins at feeding sites. Alternatively, interfering with feeding-cell development is somewhat similar to the hypersensitive response evoked by nematodes in a naturally resistant plant. Here, destruction of specific plant cells can be achieved by the localized expression of a cytotoxin such as barnase, a potent ribonuclease. This approach, however, calls for a highly specific ‘non-leaky’ promoter, which is active only in the feeding cells. Another possibility is to use a two-component system, where the leakiness of the promoter in other tissues is counterbalanced by the constitutive expression of a neutralizing gene. 相似文献
92.
Andreas Turberg Iris Schrder Susanne Wegener Michael Londershausen 《Pest management science》1996,48(4):389-398
A muscarinic acetylcholine receptor (mAChR) has been demonstrated and partially characterized in larvae of the cattle tick Boophilus microplus. Its properties are compared with mAChR from an epithelial cell line from the dipteran insect Chironomus tentans. Competition studies with cholinergic ligands of different specificity revealed the muscarinic nature of the cholinergic receptors investigated in both species. In homogenates from tick larvae, specific binding sites for [3H]quinuclidinyl benzilate (QNB) with high affinity (1·2±(0·13) nM ; Bmax 22·5 pmol mg protein−1) were detected that do not bind nicotinic compounds specifically. The estimated IC50 values for nicotine, imidacloprid and α-bungarotoxin were all in the mM range. Additionally, with tick larvae, high-affinity nicotinic binding sites were detected with [3H]nicotine which could be displaced by high concentrations of imidacloprid or QNB. The estimated IC50 values for nicotine, α-bungarotoxin, imidacloprid and QNB were 43(±8) nM , 0·8(±0·2) μM , 2·8(±0·6) μM and 78(±1·9) μM , respectively. With homogenates of the non-neuronal insect cell line from C. tentans, only high-affinity binding sites for [3H]QNB were found. Muscarinic antagonists selectively displaced [3H]quinuclidinyl benzilate (QNB) binding to tick larvae homogenates. The mAChR of B. microplus preferred pirenzepine (IC50 2·13(±1·02) μM ) among different subtype-specific mAChR antagonists (4-DAMP had IC50 49·9(±9·13) μM and methoctramine had IC50 121(±14·2) μM ) indicating a type of binding site similar to the vertebrate M1 mAChR subtype. The tick muscarinic receptor seems to be a G-protein-coupled receptor, as concluded from the 4·8-fold reduction in receptor affinity for binding of the muscarinic agonist oxotremorine M upon treatment with the non-hydrolysable GTP-analogue γ-S-GTP. Binding data for the agonists oxotremorine M (IC50 71·3(±19·6) μM ) and carbachol (IC50 253(±87·1) μM ) parallel the biological efficacy of these compounds, in that, while oxotremorine M showed some activity against ticks, carbachol was ineffective. 相似文献
93.
Robert M. REES Juliette MAIRE Anna FLORENCE Nicholas COWAN Ute SKIBA Tony van der WEERDEN Xiaotang JU 《农业科学与工程前沿(英文版)》2020,7(1):75-80
Nitrous oxide (N2O) emissions make up a significant part of agricultural greenhouse gas emissions. There is an urgent need to identify new approaches to the mitigation of these emissions with emerging technology. In this short review four approaches to precision managements of agricultural systems are described based on examples of work being undertaken in the UK and New Zealand. They offer the opportunity for N2O mitigation without any reduction in productivity. These approaches depend upon new sensor technology, modeling and spatial information with which to make management decisions and interventions that can both improve agricultural productivity and environmental protection. 相似文献
94.
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96.
Aartsma Yavanna Hao Yueyi Dicke Marcel van der Werf Wopke Poelman Erik H. Bianchi Felix J. J. A. 《Landscape Ecology》2020,35(4):907-919
Landscape Ecology - Tritrophic interactions may be affected by local factors and the broader landscape context. At small spatial scales, carnivorous enemies of herbivorous insects use... 相似文献
97.
H. A. Narouei-Khandan S. P. Worner S. L. H. Viljanen A. H. C. van Bruggen E. E. Jones 《Plant pathology》2020,69(1):17-27
Myrtle rust (caused by Austropuccinia psidii) affects more than 500 known host species in the Myrtaceae family. Three different modelling approaches (CLIMEX, MaxEnt and Multi-Model Framework) were used to project the habitat suitability for myrtle rust at both global and local scales. Current data on the global occurrence of myrtle rust were collected from online literature and expert solicitation. Long-term averages of climate data (1960–1990) were sourced from WorldClim and CliMond websites. Recent reports of myrtle rust in New Zealand were used for validation of model outputs but not in model training and testing. The model outputs were combined into a consensus model to identify localities projected to be suitable for myrtle rust according to two or three models (hotspots). In addition to the locations where the pathogen is currently present, all models successfully projected independent occurrence data in New Zealand suitable for establishment of the pathogen. Climate suitability for the pathogen was primarily related to temperature followed by rainfall in MaxEnt and the CLIMEX model. The results confirmed the optimum temperature range of this pathogen in the literature (15–25 °C). Additional analysis of the precipitation variables indicated that excessive rain (more than 2000 mm in warmest quarter of the year) combined with high temperatures (>30 °C) constrain pathogen establishment. The results of the current study can be useful for countries such as New Zealand, China, South Africa and Singapore where the pathogen has not fully spread or established. 相似文献
98.
Wynand J. Goosen Lanie Kleynhans Tanya J. Kerr Paul D. van Helden Peter Buss Robin M. Warren Michele A. Miller 《Journal of veterinary diagnostic investigation》2022,34(1):61
In South Africa, mycobacterial culture is regarded as the gold standard for the detection of Mycobacterium tuberculosis complex (MTBC) infection in wildlife even though it is regarded as “imperfect.” We compared a novel decontamination and mycobacterial culture technique (TiKa) to the conventional mycobacterium growth indicator tube (MGIT) system using known amounts of bacilli and clinical samples from MTBC-infected African buffaloes (Syncerus caffer), white rhinoceros (Ceratotherium simum), and African elephants (Loxodonta africana). Use of the TiKa-KiC decontamination agent on samples spiked with 10,000 to 10 colony forming units (cfu) of M. bovis (SB0121) and M. tuberculosis (H37Rv) had no effect on isolate recovery in culture. In contrast, decontamination with MGIT MycoPrep resulted in no growth of M. bovis samples at concentrations < 1,000 cfu and M. tuberculosis samples < 100 cfu. Subsequently, we used the TiKa system with stored clinical samples (various lymphatic tissues) collected from wildlife and paucibacillary bronchoalveolar lavage fluid, trunk washes, and endotracheal tube washes from 3 species with known MTBC infections. Overall, MTBC recovery by culture was improved significantly (p < 0.01) by using TiKa compared to conventional MGIT, with 54 of 57 positive specimens versus 25 of 57 positive specimens, respectively. The TiKa mycobacterial growth system appears to significantly enhance the recovery of MTBC members from tissue and paucibacillary respiratory samples collected from African buffaloes, African elephants, and white rhinoceros. Moreover, the TiKa system may improve success of MTBC culture from various sample types previously deemed unculturable from other species. 相似文献
99.
A modelling investigation was conducted into optimizing the number of sprays and inter-spray interval to reduce an insect population to a low level, for example, prior to pheromone trapping or the release of sterile males. The model population was age-structured and density-dependent. If spray mortality is 100% for each spray, then the ideal spraying schedule is easily determined from the durations of the various life stages. For spray mortality of less than 100%, a simulation was used to determine optimal spraying schedules. Relative length of the larval period, fertility rate and age to first oviposition were found to be the most important biotic parameters for this determination. Their importance is magnified as spray mortality decreases. The stage targeted by sprays and the percent mortality caused by each spray are also important in determining the required number of sprays. Using medfly (Ceratitis capitata Wiedmann) biotic parameters as an example when the spray targets adults, it appeared that neither the stage at which density-dependent mortality takes effect, nor the form of the adult survivorship curve are important in determining the optimal spray schedule. 相似文献
100.
Solomon Tulu TADESSE Oene OENEMA Christy van BEEK Fikre Lemessa OCHO 《农业科学与工程前沿(英文版)》2021,8(1):159-174