An improved ferritin assay for canine sera     

Andrews GA  Smith JE  Gray M  Chavey PS  Weeks BR 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1992,21(2):57-60

An improved serum ferritin assay for canine serum has been developed. It uses two monoclonal antibodies in a sandwich arrangement. Serum ferritin can be determined on undiluted canine sera with this assay. The recovery of ferritin added to canine serum ranged from 98 to 106%, the within-assay coefficient of variability was 3.3 to 4.5%, and the assay-to-assay variability was 9.8 to 10.2%. Serum ferritin from 61 apparently healthy dogs had a geometric mean of 252 ng/ml, with a range of 80 ng/ml to 800 ng/ml.  相似文献   

Identification ofTomato Mottle Taino Begomovirus strains in Cuban potato fields     

Marlene Cordero  Pedro L. Ramos  Lester Hernández  Ana I. Fernández  Ana L. Echemendía  Rudy Peral  Gloria González  Daniel García  Surey Valdés  Ana Estévez  Keren Hernández 《Phytoparasitica》2003,31(5):478-489

The presence of a begomovirus in potato plants with yellow mottle symptoms was determined for the first time in Cuba. The incidence of typical begomovirus-like symptoms in potato plants in some regions of Havana province (Güira de Melena, San José de las Lajas, Güines and Boyeros) during the growing seasons from 1992 to 1998 was in general low. However, in some cultivars belonging to the National Program for Potato Genetic Improvement, the incidence reached 100%. Yield losses, determined in 1992 and 1994, ranged as high as 19% to 56.33% depending on the cultivar. Characterization of the causal agent was done by light microscopy, host range (graft and mechanical transmission), DNA hybridizations, polymerase chain reaction, and restriction fragment length polymorphism analysis. Nucleotide sequence of the amplified fragments revealed the presence ofTomato mottle Taino virus. The virus was transmittedvia tubers and has been detected in mixed infections withPotato virus X and withPotato leaf roll virus. http://www.phytoparasitica.org posting Oct. 20, 2003. The first two authors contributed equally to this work.  相似文献   

Anatomy of the ear.     

Patricia A Heine 《Veterinary Clinics of North America: Small Animal Practice》2004,34(2):379-395

This review of the anatomy is meant to serve as a quick reference to provide the reader with a concise yet thorough overview of the anatomy of the inner, middle, and external ear as well as the physiology of hearing. Additional information is also presented regarding structures located adjacent to the ear that may be important with respect to surgical anatomy.  相似文献   

A retrospective study of visceral and nonvisceral hemangiosarcoma and hemangiomas in domestic animals.     

Patricia C Schultheiss 《Journal of veterinary diagnostic investigation》2004,16(6):522-526

Cases of hemangiosarcoma submitted to the Colorado State University Veterinary Diagnostic Laboratory during a 6-year period were reviewed. Visceral hemangiosarcomas represent less than 2% of canine specimens submitted for histologic examination and nonvisceral hemangiosarcoma less than 1%. Most nonvisceral hemangiosarcomas of dogs occur in skin. Hemangiosarcomas are less common in cats and usually occur in skin. They are also rare in other animal species. Animals with nonvisceral hemangiosarcomas are usually mature; dogs and cats average 10 years of age. The tumors develop in many different locations, and there is no sex predilection. A wide variety of dog breeds are affected, but Italian greyhounds, greyhounds, and whippets are overrepresented. Clinical outcomes of 76 cases of nonvisceral hemangiosarcomas in dogs and cats were obtained from submitting veterinarians. Completeness of excision of a tumor is the most important factor that can be used in predicting clinical outcome for an affected dog or cat. In all cases in which the animals were clinically normal for at least 1 year after surgical removal of a nonvisceral hemangiosarcoma, the margins were reported to be free of neoplastic cells. Degree of differentiation, mitotic rate, size of tumor, and presence or absence of epidermal ulceration, mast cells, or solar elastosis did not correlate with clinical outcome.  相似文献   

Adherence of Actinobacillus pleuropneumoniae serotype 1 to swine buccal epithelial cells involves fibronectin          下载免费PDF全文

Roberto Hamer-Barrera  Delfino Godínez  V. Idalia Enríquez  Sergio Vaca-Pacheco  Rodrigo Martínez-Zú?iga  Patricia Talamás-Rohana  Francisco Suárez-Güemez    Mireya de la Garza 《Canadian journal of veterinary research》2004,68(1):33-41

The swine pathogen Actinobacillus pleuropneumoniae serotype 1 was investigated for its ability to adhere to swine, rat, and human buccal epithelial cells (BEC). The highest number of bacteria adhered was to swine BEC. This binding ability was affected by heating, extreme pH, treatment with sodium dodecyl sulfate, ethylenediamine tetraacetate, or periodate, and proteolysis, suggesting that cell-surface glycoproteins participate in adherence and that adherence is based mostly on ionic interactions. Mannose and swine fibronectin may play a direct role in this interaction. Convalescent-phase serum from naturally infected pigs inhibited the adhesion. There was a correlation between bacterial pathogenicity as well as host specificity and the capacity for adherence to swine BEC. Adhesion to swine BEC provides a convenient method to study in vitro the adherence of A. pleuropneumoniae and other pathogens of the pig respiratory tract.  相似文献   

Cover Image          下载免费PDF全文

Eva Patricia López‐Damián  José Alfredo Jiménez‐Medina  Miguel Angel Lammoglia  Jaime Arturo Pimentel  Lourdes Teresa Agredano‐Moreno  Chris Wood  Carlos Salvador Galina  Tatiana Fiordelisio 《Anatomia, histologia, embryologia》2018,47(5):i-i

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Heritability of the resistance to pepper huasteco yellow vein virus in wild genotypes of <Emphasis Type="Italic">Capsicum annuum</Emphasis>     

Jesús Enrique Retes-Manjarrez  Sergio Hernández-Verdugo  Alexandre Evrard  José Antonio Garzón-Tiznado 《Euphytica》2017,213(12):275

Pepper huasteco yellow vein virus (PHYVV) is the main virus of pepper crop in Mexico. No resistant cultivars are available and resistance breeding is hampered by the lack of knowledge of heritability (h ²) of PHYVV resistance. This is a continuation of previous studies and the objectives were to analyze the h ² and the behavior of the resistant trait to PHYVV. Four resistant assays were done with three resistant wild lines (UAS12, UAS13 and UAS10) of Capsicum annuum in the S4, S5, S6 and S7 generation under greenhouse conditions. Plants from all tests were inoculated with PHYVV through Bemisia tabaci. Line UAS12 was the most resistant showing a significantly proportion of resistant plants, less disease symptoms and longer incubation time, followed by the lines UAS13 and UAS10 in all assays. Distribution of symptoms showed a bimodal tendency in all the trials, suggesting that two groups of genes are involved in this resistance trait. The lines UAS12, UAS13 and UAS10 showed the same pattern of response to selection with an average of h ² of 0.17, 0.06, 0.02 and 0.00 in the S4, S5, S6 and S7, respectively. These results indicate that all lines responded positively to the selection in the S4, S5 and S6, whereas in the S7 there was no response by the possible exhaustion of variation. Line UAS12 is the most promising genotype and the lines UAS13 and UAS10 are genetic resources that can be supplemented to breed the resistance of PHYVV. These results provides basic information for resistance breeding.  相似文献   

Preliminary insights into the incorporation of rosemary extract (Rosmarinus officinalis L.) in fish feed: influence on performance and physiology of gilthead seabream (Sparus aurata)     

A. Hernández  B. García García  M. J. Caballero  M. D. Hernández 《Fish physiology and biochemistry》2015,41(4):1065-1074

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Molecular detection of sugarcane striate virus and sugarcane white streak virus and their prevalence in the Miami World Collection of sugarcane and related grasses     

Wardatou Boukari  Denis Filloux  Jean Heinrich Daugrois  Emmanuel Fernandez  Dimitre Mollov  Claudia Kaye  Martha Hincapie  Alexander Sanchez  Jianping Wang  Philippe Roumagnac  Philippe Rott 《Plant pathology》2020,69(6):1060-1069

Viruses in the genus Mastrevirus (family Geminiviridae), including those infecting sugarcane, have natural geographical ranges almost exclusively restricted to Africa and the Indian Ocean islands off the African coast. Only sugarcane white streak virus (SWSV) in Barbados and sugarcane striate virus (SStrV) in Florida and Guadeloupe are known to infect a few sugarcane varieties in the Western Hemisphere. In this study, PCR assays were developed to detect these two viruses in sugarcane. Five hundred and seventy-one DNA samples from Saccharum species and interspecific hybrids from the Miami World Collection of sugarcane and related grasses were tested for the presence of SStrV and SWSV by PCR. No variety was found infected by SWSV but SStrV was detected in 19 varieties. PCR data were confirmed by sequencing amplified fragments (248 bp). These fragments shared 93%–100% nucleotide identity with SStrV sequences from the GenBank database. SStrV isolates were distributed in six phylogenetic groups, including the four strains of the virus. Most varieties infected by SStrV originated from Asia, thus confirming a previous hypothesis stating that this virus originated from this continent. Absence of SStrV in commercial sugarcane in Florida also suggested that this virus has not been spread in this location, while infected plants have been present for several decades.  相似文献   

Comparison between vegetation indices for detecting spatial and temporal variabilities in soybean crop using canopy sensors     

Morlin Carneiro  Franciele  Angeli Furlani  Carlos Eduardo  Zerbato  Cristiano  Candida de Menezes  Patricia  da Silva Gírio  Lucas Augusto  Freire de Oliveira  Mailson 《Precision Agriculture》2020,21(5):979-1007

Precision Agriculture - Crop monitoring through remote sensing techniques enable greater knowledge of average variability in crop growth. Canopy sensors help provide information on the variability...  相似文献