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Precision Agriculture - Crop monitoring through remote sensing techniques enable greater knowledge of average variability in crop growth. Canopy sensors help provide information on the variability...  相似文献   
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Fusarium culmorum is a haploid, worldwide occurring phytopathogenic fungus causing seedling blight, foot rot, and head blight of cereals and producing the mycotoxins deoxynivalenol (DON) and nivalenol (NIV) associated with health hazards in human and animals. The fungus reproduces asexually by conidiospores, a teleomorph is not known. We analyzed for the first time naturally occurring F. culmorum populations collected randomly in the field from infected wheat heads. A total of 186 isolates, from three populations from Germany (GER), Russia (RUS), Syria (SYR), as well as an international collection (INT) for comparison, were genotyped by 10 microsatellite (SSR, single sequence repeat) markers. A high genetic diversity within the three natural populations and the INT population as well was detected. About 90 % of multi-locus haplotypes (MLH) were unique across populations. The largest part of variance (81 %) was found within populations. Accordingly, population subdivision was low, fixation indices were significant only in one out of six comparisons, while estimates of gene flow (N m ) ranged from 0.8–4.8. Linkage equilibrium was revealed by the index of multi-locus association and the quotient of observed and expected variance when two linked markers were deleted. DON and NIV chemotypes grouped closely together in a principle coordinate analysis. SYR isolates were partly separated from GER and RUS populations. All population-genetic parameters were in a similar range compared to those for the sexually propagating species F. graminearum. In conclusion, results support the hypothesis of a recombining structure in F. culmorum as revealed by the high genetic variation within populations, a low fixation index and low gametic phase disequilibrium within populations.  相似文献   
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In Brazil, chemical control is one of the main tools to manage insect pests in wheat. However, this kind of management can impair the biological control provided by the predators Chrysoperla externa and Eriopis connexa. The present study evaluated the side effects on C. externa and E. connexa of 15 insecticides registered for wheat. Insecticides were sprayed on eggs and pupae via Potter tower at the maximum recommended dose. We evaluated the viability of eggs and pupae as well as the sub-lethal effects on fecundity and fertility of the adults emerged from treated pupae. The insecticides were classified according to the scale of toxicity proposed by IOBC. Regarding the eggs, the insecticide etofenprox was classified as slightly harmful (class 2) to C. externa. Imidacloprid + beta-cyfluthrin, diflubenzuron WG, thiamethoxam + lambda-cyhalothrin A and B, gamma-cyhalothrin, and etofenprox were also classified as slightly harmful (class 2) to E. connexa, while methomyl was classified as moderately harmful (class 3) for the eggs of the predator. The insecticide imidacloprid + beta-cyfluthrin was considered slightly harmful (class 2) to pupae of C. externa, while imidacloprid + beta-cyfluthrin, thiamethoxam + lambda-cyhalothrin A and B, and lufenuron were classified as slightly harmful (class 2) to the pupae of E. connexa. The insecticides beta-cyfluthrin, diflubenzuron SC, lambda-cyhalothrin, permethrin, thiamethoxam, triflumuron, and zeta-cypermethrin were harmless to eggs and pupae of both predators and should therefore be prioritized in the control of insect pests in wheat, thus preserving the natural biological control provided by C. externa and E. connexa.  相似文献   
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