Relationship between wheat yield and Suaeda glauca coverage on fields with different soil Na concentrations in China     

NOBUMASA ICHIZEN  TAKAYOSHI NISHIO  JINGYU SUN  XIAOJING LIU 《Weed Biology and Management》2004,4(3):168-170

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Expression of the tobacco β-1,3-glucanase gene, PR-2d, following induction of SAR with Peronospora tabacina     

Deanna L. Funnell  Christopher B. Lawrence  Jeffrey F. Pedersen  Christopher L. Schardl 《Physiological and Molecular Plant Pathology》2004,65(6):285-296

Systemic acquired resistance (SAR) is induced following inoculation of Peronospora tabacina sporangia into the stems of Nicotiana tabacum plants highly susceptible to the pathogen. Previous results have shown that accumulation of acidic β-1,3-glucanases (PR-2's) following induction of SAR by P. tabacina may contribute to resistance to P. tabacina. We showed that up-regulation of the PR-2 gene, PR-2d, following stem inoculation with P. tabacina, is associated with SAR. Studies using plants transformed with GUS constructs containing the full length promoter from PR-2d or promoter deletions, provided evidence that a previously characterized regulatory element that is involved in response to salicylic acid (SA), may be involved in regulation of PR-2d following induction of SAR with P. tabacina. This work provides evidence that regulation of PR-2 genes during P. tabacina-induced SAR may be similar to regulation of these genes during infection of N-gene tobacco by TMV or following exogenous application of SA, and provides further support for the role of SA in regulation of genes during P. tabacina-induced SAR.  相似文献   

Determination of deoxynivalenol- and nivalenol-producing chemotypes of Fusarium graminearum isolated from wheat crops in England and Wales     

P. Jennings †  M. E. Coates  K. Walsh  J. A. Turner  P. Nicholson 《Plant pathology》2004,53(5):643-652

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西安荷斯坦奶牛群5个基因座位遗传多态性的PCR-RFLP分析   总被引：6，自引：0，他引：6  

张润锋  陈宏  蓝贤勇  田燚  潘传英  胡沈荣  苏利红 《畜牧兽医学报》2005,36(6):545-549

应用PCR-RFLP方法对西安荷斯坦牛的κ-en、β-lg、β-lg5′侧翼区、CSN1S2、IGFBP-3共5个基因座位进行了多态性分析。结果表明，在西安荷斯坦牛群中，没有发现携带CSN1S2^P等位基因的个体，其多态信息含量为0。κ-en基因座位呈现低度多态(PIC=0．2366)，β-lg、β-lg5′侧翼区、IGFBP-3基因座位的多态信息含量分别为0．3168、0．3689、0．4439，均呈现中度多态。κ-en、β-lg、β-lg5′侧翼区、IGFBP-3、CSNIS2基因座位的杂合度和DNA多态度分别为0．2742、0．3947、0．4879、0．4891、0和0．0255、0．0116、0．0333、0．0112、0。而且，在西安荷斯坦牛群中，κ-en、β-lg、β-lg5′侧翼区、IGFBP-3共4个基因座位均处于Hardy-Weinberg平衡状态，CSN1S2基因座位处于纯合状态。  相似文献   

亚洲璃眼蜱唾液腺一新功能基因的克隆与测序   总被引：1，自引：0，他引：1  

程天印  周金林  施启顺  周勇志  林矫矫 《畜牧与兽医》2005,37(5):4-6

HaB1是亚洲璃眼蜱雌成蜱唾液腺差异表达基因文库中的一个片段,根据其序列设计引物HaB1-GSP1和HaB1-GSP2,以唾液腺总RNA为模板,RACE法扩增获得HaB1的未知3′-末端。测定该末端序列,进行序列拼接,设计全长引物5-′CCAGTCCGAAGGAAGGGCG-3′和5-′TCTC-CGGGCACGTGAAGTGTC-3′。以cDNA第一链为模板,扩增获得基因全长。经核查表明,该基因为一新基因(登录号AY803896)。  相似文献   

鸭流感病毒4/2004-H6N2亚型毒株的血凝素基因全序列克隆与分析     

顾节清 陈杰李晓成  杨增岐吴发兴张燕霞  黄保续 《中国动物检疫》2005,22(5):31-33

2004初从正常鸭群中分离到一株鸭源禽流感病毒，命名为A／Duck／HN／4／2004(H6N2)。经对血凝素基因(HA)序列分析发现HA基因全长为1744bp，共编码566个氨基酸，在裂解位点仅含一个碱性氨基酸-精氨酸(R)，符合LPAIV的标准。将所得基因序列与已发表的同一亚型参考序列分析表明，与H6亚型流感HA基因同源性为89．2％-97．1％，经分子遗传演化分析表明本次分离株与香港分离株A／Duck／Hong Kong／3600／99(H6N2)、A／Duck／Hong Kong／3600／99(H6N2)最近。  相似文献   

毒害艾美耳球虫(Eimeria necatrix)广东株微线蛋白-2基因在大肠杆菌的表达     

覃宗华  谢明权  蔡建平  艾哈迈德  彭新宇  魏文康  吴惠贤 《中国兽医学报》2005,25(1):22-25

根据克隆的毒害艾美耳球虫(Eimeria necatrix)广东株微线蛋白-2基因(EnMIC-2(Gd))的cDNA序列设计特异性引物，用PCR方法扩增其阅读框架(ORF)后，克隆至质粒表达载体pET-32a( )，成功构建了重组表达质粒pET-32a( )-EnMIC-2。用CaCl2法将其转化至宿主细菌E.cliBL21(DE3)，并用IPTG成功诱导了EnMIC-2重组抗原在大肠杆菌表达。表达的重组蛋白约占菌体总蛋白的10．8％，其相对分子质量约为55000。重组蛋白经SDS-AGE分析后，用E．necatrix(广东株)感染鸡的高免血清进行Western Blotting分析，结果为阳性。  相似文献   

Validation of masks for determination of V̇O2max in horses exercising at high intensity          下载免费PDF全文

R. H. Sides  R. Kirkpatrick  E. Renner  K. Gough  L. M. Katz  D. L. Evans  W. M. Bayly 《Equine veterinary journal》2018,50(1):91-97

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Validation of biplane high‐speed fluoroscopy combined with two different noninvasive tracking methodologies for measuring in vivo distal limb kinematics of the horse          下载免费PDF全文

S. M. Geiger  E. Reich  P. Böttcher  S. Grund  J. Hagen 《Equine veterinary journal》2018,50(2):261-269

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Canine GM2‐Gangliosidosis Sandhoff Disease Associated with a 3‐Base Pair Deletion in the HEXB Gene          下载免费PDF全文

P. Wang  P.S. Henthorn  E. Galban  G. Lin  T. Takedai  M. Casal 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(1):340-347

Background

GM2‐gangliosidosis is a fatal neurodegenerative lysosomal storage disease (LSD) caused by deficiency of either β‐hexosaminidase A (Hex‐A) and β‐hexosaminidase B (Hex‐B) together, or the GM2 activator protein. Clinical signs can be variable and are not pathognomonic for the specific, causal deficiency.

Objectives

To characterize the phenotype and genotype of GM2‐gangliosidosis disease in an affected dog.

Animals

One affected Shiba Inu and a clinically healthy dog.

Methods

Clinical and neurologic evaluation, brain magnetic resonance imaging (MRI), assays of lysosomal enzyme activities, and sequencing of all coding regions of HEXA, HEXB, and GM2A genes.

Results

A 14‐month‐old, female Shiba Inu presented with clinical signs resembling GM2‐gangliosidosis in humans and GM1‐gangliosidosis in the Shiba Inu. Magnetic resonance imaging (MRI) of the dog's brain indicated neurodegenerative disease, and evaluation of cerebrospinal fluid (CSF) identified storage granules in leukocytes. Lysosomal enzyme assays of plasma and leukocytes showed deficiencies of Hex‐A and Hex‐B activities in both tissues. Genetic analysis identified a homozygous, 3‐base pair deletion in the HEXB gene (c.618‐620delCCT).

Conclusions and Clinical Importance

Clinical, biochemical, and molecular features are characterized in a Shiba Inu with GM2‐gangliosidosis. The deletion of 3 adjacent base pairs in HEXB predicts the loss of a leucine residue at amino acid position 207 (p.Leu207del) supporting the hypothesis that GM2‐gangliosidosis seen in this dog is the Sandhoff type. Because GM1‐gangliosidosis also exists in this breed with almost identical clinical signs, genetic testing for both GM1‐ and GM2‐gangliosidosis should be considered to make a definitive diagnosis.  相似文献