陆地棉与7个野生棉杂种细胞学研究   总被引：2，自引：0，他引：2  

王坤波  李懋学 《作物学报》1996,22(1):27-35

分析了四倍体栽培种陆地棉与辣根棉、哈克尼西棉、旱地棉、雷蒙德氏棉、裂片棉、异常棉和司笃克克棉等二倍体野生棉种间杂种Ｆ１的细胞学，花粉母细胞中期Ｉ染色体平均构型依次为：１４．６２Ⅰ＋１１．９３Ⅱ＋０．１７Ⅲ、１４．２１Ⅰ＋１２．３６Ⅱ＋０．０２Ⅲ、１１．７０Ⅰ＋１３．５２Ⅱ＋０．０９Ⅲ、１３．５６Ⅰ＋１２．７２Ⅱ、１４．１０１＋１２．２７Ⅱ＋０．１２Ⅲ、２８．１９Ⅰ＋５．１６Ⅱ＋０．１６Ⅲ和３１．２  相似文献   

Colchicine-induced chromosome doubling in Platanus acerifolia and its effect on plant morphology   总被引：1，自引：0，他引：1  

Guofeng Liu  Zhineng Li  Manzhu Bao 《Euphytica》2007,157(1-2):145-154

We report the production of tetraploid plants of Platanus acerifolia, with the ultimate aim of improving the ornamental qualities of this important urban landscaping tree. Chromosome doubling was achieved by the application of colchicine to either pre-soaked seed or to the apical meristems of young seedlings. Treatment of the ungerminated seed was the more efficient method in terms of numbers of tetraploid seedlings (up to 40%, as determined by chromosome counting of the root-tip nuclei) but this method produced no mature tetraploid plants due to the deleterious effect of colchicine on subsequent root growth. When colchicine was applied directly to the apical growing tip of cotyledon-stage seedlings, leaf and stem growth was temporarily affected but the plants eventually recovered. We conducted a preliminary screen for putative tetraploids based on the observation in other plant species of a correlation of stomatal size and distribution with ploidy. Plants containing significantly larger stomata and at a lower density across the lower leaf epidermis, were selected for further analysis by flow cytometry and chromosome counting. These techniques confirmed that, of the 12 putative polyploids, four were tetraploid, five were mixoploid and three were, in fact, diploid. Morphological differences of the tetraploids included a more compact growth habit and broader, thicker leaves. These plants are being grown to full maturity in order to test their potential for use in a breeding programme aimed at producing sterile triploid lines.  相似文献   

小麦7D染色体数量性状基因定位和效应估计的研究   总被引：10，自引：0，他引：10  

李维明 Worl.  AJ 《作物学报》1996,22(6):641-646

综合应用方差分析法、区间定位法和联合定位法将控制抽穗期、有效穗数、小穗数、５０粒重和单穗产量等５个性状的６个数量性状基因座（ＱＴＬ）定位在小麦７Ｄ染色体上。其中控制粒重的ＱＴＬ有２个。这些ＱＴＬ集中分布在着丝粒附近和染色体长臂的末端两个区域。效应估计结果表明多数ＱＴＬ的效应是微效的，控制同一个性状（粒重）的两个ＱＴＬ的效应几乎相等。比较３种ＱＴＬ定位法的分析结果，方差分析简单有效，且当染色体上只有  相似文献   

Staining of heterochromatin bands and the determination of rye chromosomes in triticale (wheat × rye hybrid)     

Val T. Sapra  Moira D. Stewart 《Euphytica》1980,29(2):497-509

Summary Fifteen lines of triticale were studied using the C-banding staining method. A typical, complete chromosomal complement was found in 11 lines, while the remaining lines demonstrated some variations. A slight modification in the technique is discussed.Supported in part by CSRS/USDA Grant No. 801-15-03A.  相似文献   

大麦幼胚培养再生植株及其后代的细胞遗传学研究   总被引：3，自引：0，他引：3  

袁妙葆  张毓芳 《作物学报》1991,17(3):171-177

从5个普通大麦品种的幼胚培养中均得到体细胞再分化植株。对214棵再生植株进行了细胞学检查,发现四倍体5株,占总数的2.3%;相互易位杂合体1株,占总数的0.46%。四倍体植株虽然得到少数大而瘪的种子,但它们次年没有发芽;易位杂合体减数分裂时的染色体构型为5Ⅱ+1Ⅳ,属于两对非同源染色体间的简单易位。其余所有二倍体植株的细胞  相似文献   

Wheat starch biosynthesis     

M.K. Morell  S. Rahman  A. Regina  R. Appels  Z. Li 《Euphytica》2001,119(1-2):55-58

Starch biosynthesis in plants involves the concerted action of a numberof enzymes, including ADPglucose pyrophosphorylase, starch synthases,branching enzymes and debranching enzymes. We report on the cloningand characterisation of genes encoding these enzymes from wheat and ontheir chromosomal locations. The prospects for manipulating wheat starchstructure and functionality using these genes is discussed.  相似文献   

陆地棉与三裂棉、斯特提棉和A111种间杂种F1的形态与细胞学   总被引：2，自引：0，他引：2  

周宝良  彭跃进  沈新莲  黄骏麒  钱思颖 《作物学报》1996,22(1):84-88

本文研究了TM-1×三裂棉、江苏棉1号×斯特提棉、泗棉2号×Alll 3个种间杂种F_1的主要形态特征及花粉母细胞减数分裂中期Ⅰ的染色体行为。3个种间杂种F_1的花器官性状大多趋向于父本野生棉,如花冠颜色、花心等;营养器官大多趋向于中间型,如叶色、叶形等。杂种F_1均表现高度不育。减数分裂中期Ⅰ的染色体构型依次为:13.73Ⅰ 12.52Ⅱ 0.03Ⅲ 0.04Ⅳ,28.03Ⅰ 5.38Ⅱ 0.07Ⅲ和33.26Ⅰ 2.87Ⅱ;其染色体组亲和性指数分别为0.9715、0.4192和0.2208。这一结果表明:(1)陆地棉与三裂棉的亲缘关系较近,与斯特提棉的亲缘关系较远,与Alll的亲缘关系更远;(2)泅棉2号×Alll的F_1染色体数为2n=3x=39,其染色体数x=13与棉属相同,其次因Alll原产于澳大利亚,形态上与纳尔逊氏棉、澳洲棉比较接近,因此推测它可能属于棉属的C染色体组种。  相似文献   

Analysis of wheat-Thinopyrum intermedium derivatives with BYDV-resistance     

Zhi-Shan Lin  Zhi-Fu Cui  Xiang-Yan Zeng  You-Zhi Ma  Zeng-Yan Zhang  Toshiki Nakamura  Goro Ishikawa  Kazuhiro Nakamura  Hisashi Yoshida  Zhi-Yong Xin 《Euphytica》2007,158(1-2):109-118

Chromosome compositions of seven lines, derived from hybrids between a wheat cultivar and the wheat-Thinopyrum intermedium addition line Z6, with barley yellow dwarf virus (BYDV) resistance, were determined by genomic in situ hybridization, cytogenetic and SSR assays. The results showed that line N522 was a disomic addition line, lines N420 and N439 were 2Ai-2(2B) chromosome substitution lines, lines N431 and N452 were 2Ai-2(2D) chromosome substitution lines, line N523 was a 2Ai-2S(2D) ditelosomic substitution line, and line N530 was a double ditelosomic line with the mitotic chromosome number of 2n = 40 + 4t. One pair of telosomes in line N530 lacked several proximal SSR markers of chromosome 2AS, but possessed certain terminal markers, which were consistent with an acrocentric structure, and the other pair of chromosome arms were presumably 2Ai-2S telosomes with BYDV-resistance. These wheat-Th. intermedium lines provide useful genetic resources for developing alien chromosome translocation lines.  相似文献   

稻属间杂种花粉植株的细胞遗传学研究   总被引：1，自引：0，他引：1  

颜辉煌  胡慧英 《作物学报》1997,23(6):689-692

以栽培稻０２４２８与紧穗野生稻间不育Ｆ１杂种（２ｎ＝２４，ＡＣ）为材料进行花约培养，共接种花约３２６８８个，获得再生绿苗１３株及白化苗两株，愈伤诱导率与分化率分别为０．１４％和３４．７８％。有９朱花粉绿苗长至成熟，均表现长芒、紫柱头、强感光及易落粒等野生亲本的特性。细胞学结果进一步表明，其中４株为二倍体（２ｎ＝２４），形态上琚的Ｆ１杂种相似，花粉母细胞中期Ⅰ亦只能见到少数二价体；另５株则为四倍体（  相似文献   

20℃下3种蔬菜种子长年贮存后生理特性和遗传稳定性的研究   总被引：4，自引：0，他引：4  

孟淑春  张海英  刘庞沅  孔祥辉 《华北农学报》2003,18(4):26-30

对在低温和常温下贮藏13年的萝卜、番茄和芹菜3种蔬菜超干种子的发芽势、发芽率、苗长、胚根根尖细胞染色体畸变率和随机扩增的多态性DNA(RAPD)等指标进行测定分析，并研究了种子活力、生活力及其遗传稳定性的变化。多年超干贮存后，部分处理染色体异常的百分率有所增加，它与发芽率成反比，但发芽率低的种子在繁殖更新后，异常率会降低，较高的染色体异常率不会遗传下去。  相似文献