Comparison of flagellin and an oil-emulsion adjuvant in inactivated Newcastle disease vaccine in stimulation of immunogenic parameters     

《Comparative immunology, microbiology and infectious diseases》2021

The present study was designed to investigate the potential application of native (N) and recombinant (truncated modified [tmFliC] and full-length [flFliC]) flagellin proteins along with inactivated Newcastle disease virus (NDV). Fifty six SPF chickens were immunized twice with PBS (control), inactivated NDV (Ag), inactivated NDV/flFliC (AgF), inactivated NDV/tmFliC (AgT), inactivated NDV/N (AgN), commercial vaccine containing Montanide (Vac) and Vac/N (VacN), with a two-week interval. Blood was collected weekly and spleens were harvested after chickens were sacrificed. Interleukin-6 (IL-6) and tumor necrotic factor-α (TNF-α) gene expression in peripheral blood mononuclear cells were analyzed by Real-Time PCR. Antibody response was assessed by haemagglutination inhibition (HI). Cellular activity was quantified by MTT assay. Results showed that the most IL-6 and TNF-α gene expression was observed in AgF group (P < 0.01). The lowest gene expression among vaccinated groups was observed in Ag group for IL-6 and Ag and Vac group for TNF-α. The highest HI titer was observed in Vac, VacN, AgF and AgT groups. The AgF group showed the highest cellular activity (P < 0.01). In conclusion, flagellin-adjuvanted groups showed a pro-inflammatory effect and acted similarly to or better than the Vac group. Hence, flagellin can be proposed as a potential adjuvant for ND vaccine.  相似文献   

Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens          下载免费PDF全文

ZENG Xian-ying  CHEN Xiao-han  MA Shu-jie  WU Jiao-jiao  BAO Hong-mei  PAN Shu-xin  LIU Yan-jing  DENG Guo-hua  SHI Jian-zhong  CHEN Pu-cheng  JIANG Yong-ping  LI Yan-bing  HU Jing-lei  LU Tong  MAO Sheng-gang  GUO Xing-fu  LIU Jing-li  TIAN Guo-bin  CHEN Hua-lan 《农业科学学报》2020,19(9):2294-2300

We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5 N6)(DK/GZ/S4184/17)(a clade 2.3.4.4 d virus), A/chicken/Liaoning/SD007/2017(H5 N1)(CK/LN/SD007/17)(a clade 2.3.2.1 d virus), and A/chicken/Guangxi/SD098/2017(H7 N9)(CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7 N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7 N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.  相似文献   

Construction of recombinant capripoxviruses as vaccine vectors for delivering foreign antigens: Methodology and application     

《Comparative immunology, microbiology and infectious diseases》2019

Goatpox (GTP), sheeppox (SPP) and lumpy skin disease (LSD) are three severe diseases of goat, sheep and cattle. Their typical clinical symptoms are characterized by vesicles, papules, nodules, pustules and scabs on animal skins. The GTP, SPP and LSD are caused by goatpox virus (GTPV), sheeppox virus (SPPV) and lumpy skin disease virus (LSDV), respectively, all of which belong to the genus Capripoxvirus in the family Poxviridae. Several capripoxvirus (CaPV) isolates have been virulently attenuated through serial passaging in vitro for production of live vaccines. CaPV-based vector systems have been broadly used to construct recombinant vaccines for delivering foreign antigens, many of which have been demonstrated to induce effective immune protections. Homologous recombination is the most commonly used method for constructing recombinant CaPVs. Here, we described a methodology for generation of recombinant CaPVs by the homologous recombination, and further reviewed CaPV-vectored vaccines for delivering foreign antigens.  相似文献   

羊源链球菌的分群鉴定及多价灭活苗的研制   总被引：1，自引：0，他引：1  

欧阳素贞  王双山  王国栋  刘书梅  梁秀丽  蔡敏 《中国预防兽医学报》2004,26(6):448-454

2000～2003年,从安阳、邯郸等6个地区采取280份羊的病料,从中分离到150株链球菌,对其中20株典型分离株进行了生化分群鉴定及兰氏分群A～G诊断试剂鉴定.结果表明,6个地区发生的羊的链球菌病主要由C群兽疫链球菌(7/20),D群(8/20),E群(2/20)引起.其分布无明显地域性.选择C、D、E群中各1株为菌种,制备多价链球菌灭活苗,通过免疫试验,保护率达92%以上,免疫期半年以上,疫苗4℃保存期1年以上,证明该灭活苗安全性好,免疫效果明显.  相似文献   

鸡的微卫星DNA标记与胴体性状的相关分析   总被引：4，自引：1，他引：3  

陈晖  郑丽祯  陈岩锋  郑嫩珠  缪中纬  陈宽维  汤青萍 《中国家禽》2004,8(Z1):137-140

测定了青脚麻鸡与隐性白鸡杂交后自交的F2代个体的全净堂重、全净堂率、脂肪宽带、腹脂重和腹脂率6个胴体性状,检测10个微卫星DNA标记,对标记基因型胴体性状值进行方差分析和多重比较,结果表明:MCW328对于10周龄体重和全净膛重两性状各基因型均有显著的差异(P<0.05);ADL292对于腹脂重性状各基因型有极显著的差异(P<0.01),对于腹脂率有显著的差异(P<0.05)。MCW328标记基因与控制10周龄体重和全净膛重QTL连锁,ADL292标记基因与控制腹脂重和腹脂率QTL连锁。  相似文献   

猪口蹄疫O型灭活疫苗PD50效检攻毒方式探索   总被引：2，自引：2，他引：0  

郎洪武  闫岩  张荣军  肖怀红  杨汉春 《中国兽药杂志》2004,38(3):26-27

用10 000 TCID50的OR/80株F13细胞毒或用10 000 LD50的OR/80株乳鼠组织毒(ORMF8),通过肌肉注射途径、蹄踵球部或趾间皮内注射途径接种O型口蹄疫抗体阴性架子猪进行攻毒试验,三种途径均不能使试验猪产生规律性发病.  相似文献   

S1核酸酶突变检测法的优化     

李春雨 王涛梁本国  李光晨 《勤云标准版测试》2004,(5)

优化了S1核酸酶突变检测体系,结果表明(1)扩增法比杂交法产生异源双链DNA更节省时间;(2)PCR产物可以直接作为突变检测的底物;(3)适当降低反应温度、适当增加反应缓冲液中的NaCl浓度、适当缩短反应时间可提高突变检测效果。  相似文献   

蓝舌病灭活疫苗免疫羊ELISA和琼扩监测比较试验     

陈兴扶  范根成  马洪超  杨承谕  周勇  曹正惠  陈远坤  段金荣  冷光敏  郑贡诗 《中国动物检疫》1994,11(3):10-11

用19只绵羊（注苗前BTV琼扩和ELISA均为阴性）注射BTV灭活苗后7～139天的血清进行BTVELISA和琼扩平行试验，结果表明，注苗后7～30天11只试验丰ELISA阳性，注苗40天13只羊ELISA阳性，注苗90天以后全部试验羊只均为ELISA阳性，而琼扩试验则始终为阴性结果。  相似文献   

鸭疫里默氏杆菌病的防治     

邓朝阳  黄夏  熊毅  磨龙春  何贻坚 《畜牧兽医杂志》2003,22(6):1-3

鸭浆膜炎是由鸭疫里默氏杆菌引起的传染病，该菌有21个血清型。本试验通过对发病鸭的临床诊断和病原进行分离鉴定。用本场的菌株制作灭活苗进行预防免疫，取得了很好的防治效果，保护率高达90％以上。  相似文献   

马传贫驴白细胞弱毒疫苗株跨膜蛋白主要免疫决定区基因的克隆与表达   总被引：3，自引：0，他引：3  

薛飞  贾斌  朱远茂  杨建德  王晓钧  相文华  赵立平  吕晓玲  沈荣显 《中国预防兽医学报》2003,25(5):321-325

从感染驴白细胞的马传贫驴白细胞弱毒疫苗株前病毒DNA中克隆了编码跨膜蛋白主要免疫决定区(TMIR)的基因，并在大肠杆菌中进行了表达。所表达的融合蛋白有一部分是可溶的，其氨基端带有6个组氨酸的标签，因此可以用固定化金属离子亲和层析法在非变性条件下进行纯化。在间接酶联免疫吸附试验(ELISA)和免疫印迹试验中，重组的TMIR蛋白可与马传贫阳性血清样品发生反应，而与健康马血清无任何反应。这表明该重组蛋白具有良好的抗原性和特异性，可用于马传贫弱毒疫苗株在体内外复制、接种马体内免疫应答及马传贫诊断的研究。  相似文献