Comparative Analysis of the Outer Membrane Protein Profiles of Isolates of the Pasteurella multocida (B:2) Associated with Haemorrhagic Septicaemia     

Tomer P  Chaturvedi GC  Minakshi  Malik P  Monga DP 《Veterinary research communications》2002,26(7):513-522

Outer membrane proteins (OMP) of P. multocida (serotype B:2) field isolates (n = 6) and a vaccine strain (P-52) were extracted by a sarkosyl method and characterized using SDS-PAGE and immunoblotting. About 20 polypeptide bands were observed in the profile of the vaccine strain with MW ranging from 16 to 90 kDa and, based on band thickness and intensity of staining, three polypeptides of MW 31, 33 and 37 kDa were considered to be the major OMPs. The profiles of the field isolates showed minor differences when compared with that of the vaccine strain. The OMP of 33 kDa was only expressed by the vaccine strain. Four field isolates expressed an OMP of 39 kDa, which did not appear in the profiles of the remaining two field isolates and the P-52 strain. Similarly, an OMP of 25 kDa was exclusively seen in the profile of a single isolate. By immunoblotting studies, using anti-P. multocida (P-52) whole-cell hyperimmune serum raised in rabbits as well as buffalo immune sera, it became evident that the polypeptide of 37 kDa was the most antigenic OMP in the profiles of all the isolates, including the P-52 strain. Other polypeptides were either weakly antigenic or visible in the profile of only a few of the isolates. The study thus identified the major OMP of P. multocida (B:2) and suggested that this highly antigenic 37 kDa OMP has potential for further protective and immunodiagnostic studies.  相似文献   

应用16S rRNA基因测序法鉴定禽多杀性巴氏杆菌的研究   总被引：4，自引：0，他引：4  

管宇  沈志强  刘吉山  庞万勇  王燕  莫玲 《中国预防兽医学报》2003,25(5):349-352

应用 16SrRNA基因测序法对以疫苗标准强毒株C4 8_1和弱毒疫苗代表株G190E4 0为阳性对照株和分离鉴定的 2株禽源多杀性巴氏杆菌Chicken/guangxi/ 2 0 0 0_1、Chicken/guangdong / 2 0 0 2_1株 ,进行 16SrRNA基因序列分析。结果表明 ,2株分离菌与对照的强弱毒株之间的同源性为 10 0 %。后经Blastn分析Chicken/guangxi/ 2 0 0 0_1、Chicken/guangdong /2 0 0 2_1、C4 8_1、G190E4 0株与已发表 11株多杀性巴氏杆菌同源性高达 10 0 % ,与已发表的 34株多杀性巴氏杆菌同源性均超过 98% ,同时与其它巴氏杆菌种如溶血巴氏杆菌等同源性均低于 96 % ,进一步证实Chicken/guangxi/ 2 0 0 0_1、Chicken/guangdong / 2 0 0 2_1分离株均为多杀性巴氏杆菌。生化实验鉴定表明Chicken/guangxi/ 2 0 0 0_1、Chicken/guangdong / 2 0 0 2_1、C4 8_1、G190E4 0菌株均为subsp .Multocida亚种。  相似文献   

兔巴氏杆菌-大肠杆菌油乳与中药灭活苗的研制     

王淑芳  刘一尘  薛帮群  李爱江  李宏伟  任章留 《中国养兔杂志》2003,(4):17-19

以多杀性巴氏杆菌(C-481)、致病性大肠杆菌O85、O119、O18等菌株制备的兔巴氏杆菌-大肠杆菌油乳与中药灭活菌苗，经实验室免疫试验和中间试验证明，该菌苗在免疫后第14天即可产生坚强的免疫力；经攻毒试验证明，该菌苗的保护率为93％，免疫期为9个月，免疫效果良好。该菌苗在4℃以下可保存18个月以上，室温阴暗处可保存10个月以上。临床使用证明，该菌苗能有效的控制巴氏杆菌病与大肠杆菌病在兔群中的流行，可在生产中推广应用。  相似文献   

乌苏里貉巴氏杆菌的分离鉴定     

刘苓钰  黎梅  王树志  杜锐 《经济动物学报》2007,11(4):243-245

吉林省松原市某养貉场发生疫病,对病死貉进行剖检,见有典型的巴氏杆菌病的症状,然后对细菌进行分离培养和生化鉴定,证实引起貉死亡的是巴氏杆菌病。  相似文献   

鹅巴氏杆菌病的病原分离鉴定及其病理学观察     

刘丽颖  贺文琦  陆慧君  宋德光  赵魁  高丰 《动物医学进展》2007,28(10):33-37

通过镜检、培养、琼扩试验、生化鉴定及动物回归等试验,从临床表现为神经症状、眼观主要病变为肝肿大、肝表面和切面有灰白色坏死灶为主要特征的病鹅体内分离出禽多杀性巴氏杆菌,其主要病理组织学变化为部分肝小叶内含有一个或多个凝固性坏死灶;大脑皮层血管周围有多量的中性粒细胞浸润,形成明显"血管套"现象,呈化脓性脑炎变化.通过电镜观察、SPF鸡胚接种和血凝抑制试验等排除了禽流感、新城疫等病毒感染的可能性,为禽巴氏杆菌病的诊断提供了资料.  相似文献   

某养鸡场禽霍乱分离菌对常用抗菌药物的敏感性研究     

徐海军  周科伟 《家畜生态》2006,(3)

从六安地区某养鸡场禽霍乱病鸡上分离到1株禽多杀性巴氏杆菌,采用纸片扩散法和试管稀释法分别测定了青霉素钾、土霉素、硫酸卡那霉素、硫酸庆大霉素、硫酸链霉素、复方新诺明、甲砜霉素、氟哌酸、环丙沙星和恩诺沙星等10种药物对分离菌的体外抗菌活性。结果表明分离到的禽多杀性巴氏杆菌对青霉素钾和土霉素耐药;对硫酸卡那霉素、硫酸庆大霉素、硫酸链霉素、甲砜霉素、氟哌酸敏感;对复方新诺明、环丙沙星和恩诺沙星极敏感。两种测定方法得到的结果一致。因此复方新诺敏、环丙沙星和恩诺沙星可作为目前该发病场防治禽霍乱的首选药物。  相似文献   

巴氏杆菌对链霉素和磺胺耐药机制的研究   总被引：1，自引：0，他引：1  

金天明  高丰  佟庆彬  武迎红 《畜牧与兽医》2007,39(2):5-7

根据巴氏杆菌的链霉素耐药基因StrA和磺胺耐药基因SulⅡ序列设计2对引物,以临床分离的4株禽源巴氏杆菌耐药株为模板,扩增出StrA、SulⅡ基因序列。经克隆和核苷酸序列测定,克隆的基因与文献报道的耐药基因序列(NC-001774)有较高的同源性(≥98.9%);试验结果显示,耐药株巴氏杆菌对链霉素和磺胺耐药性的差异与StrA、SulⅡ基因突变有关(耐药性高的菌株基因突变率高)。试验中所有耐药菌均在StrA基因编码的第204位氨基酸处存在突变;所有耐药菌均在SulⅡ基因编码的第159,246,384,584,590,591,597,582位氨基酸处存在突变,上述突变基因可能是导致巴氏杆菌对链霉素和磺胺产生耐药性的主要因素。  相似文献   

鸡IL-18对禽多杀性巴氏杆菌DNA疫苗的免疫佐剂作用     

曹素芳  黄青云 《中国兽医科技》2007,37(12):1058-1061

为了探索鸡IL-18在禽多杀性巴氏杆菌H基因DNA疫苗中的免疫佐剂作用，分别用共表达鸡IL-18基因和禽多杀性巴氏杆菌C48-1H基因的DNA疫苗、鸡IL-18真核表达质粒pcDNA3／cIL-18与禽多杀性巴氏杆菌C48-1H基因的DNA疫苗混合物、禽多杀性巴氏杆菌C48-1H基因的DNA疫苗肌肉注射5周龄鸡，首免后每周采取外周血及外周抗凝血，应用ELISA和MTT法分别检测免疫鸡的体液免疫及细胞免疫水平。二免后第2周用10 LD50禽多杀性巴氏杆菌强毒菌株C48-1进行攻击。结果鸡IL-18能够明显增强禽多杀性巴氏杆菌H基因DNA疫苗的免疫原性，显著提高免疫鸡的体液免疫和细胞免疫水平，并且鸡IL-18与禽多杀性巴氏杆菌H基因共表达时的免疫佐剂作用最强，能强有力地抵抗强毒菌株C48-1的致死性攻击。结果表明，鸡IL-18可作为DNA疫苗的一种理想的免疫佐剂。  相似文献   

猪源D型产毒素多杀性巴氏杆菌toxA基因的克隆与表达   总被引：5，自引：0，他引：5  

王大鹏  吴斌  周锐  唐先春  陈焕春 《中国兽医学报》2006,26(3):271-274

皮肤坏死毒素（Dermonecrotic toxin，DNT）是产毒素多杀性巴氏杆菌（Toxigenic Pasteurella multocida，T^＋Pm）的主要毒力因子和保护性抗原。以猪源D型T^＋Pm的基因组DNA为模板，用PCR方法扩增得到了编码DNT的toxA全基因编码序列，共4019bp。将其克隆到pMD18-T载体并测序，结果表明，toxA基因序列与GenBank已报道的5个toxA基因序列的同源性达99．8％以上。将该基因亚克隆到原核表达载体pGEX-KG的GST基因下游，转化BL21（DE3）大肠杆菌，经IPTG诱导表达，获得大小约173000的融合蛋白。Western blot结果表明，该融合蛋白具有良好的反应原性。动物试验表明，该重组蛋白可以诱导小鼠产生高水平的抗体，并可抵抗致死剂量的天然DNT毒素攻击。  相似文献   

Experimental studies of chronic pneumonia of sheep     

J.S Gilmour  G.E Jones  A.G Rae 《Comparative immunology, microbiology and infectious diseases》1979,1(4):285-293

Strains of Mycoplasma ovipneumoniae and Pasteurella haemolytica isolated from sheep affected with chronic pneumonia were inoculated by endobronchial route to conventionally-reared and SPF (Specific Pathogen-Free) lambs. Changes resembling those of the naturally-occurring disease were produced in most lambs given the organisms in combination and in some given M. ovipneumoniae alone. Similar but less extensive changes were seen in SPF lambs and fewer animals were affected. Different strains of M. ovipneumoniae did not affect the extent of changes produced in SPF lambs. M. ovipneumoniae became established in the lungs of both types of sheep; P. haemolytica did so less readily.

It was concluded that chronic pneumonia may be reproduced in conventional animals by combined inoculation of M. ovipneumoniae and P. haemolytica. Age and status of immunity to mycoplasmas may account for the different responses of conventional and SPF lambs.  相似文献