Cartilage-derived retinoic acid-sensitive protein in equine synovial fluid from healthy and diseased joints     

Berg LC  Lenz J  Kjelgaard-Hansen M  Thomsen PD  Jacobsen S 《Equine veterinary journal》2008,40(6):553-557

Reasons for performing study: More sensitive and specific diagnostic methods for early detection of changes in the joint cartilage are needed. Cartilage‐derived retinoic acid‐sensitive protein (CD‐RAP) is a potential marker of cartilage synthesis and regeneration. This is the first study on equine CD‐RAP. Objectives: To evaluate the ability of a commercially available human sandwich ELISA assay to detect equine CD‐RAP in synovial fluid from healthy and diseased joints. Methods: Synovial fluid was collected from 28 horses with no signs of joint disease and from 5 with induced inflammatory arthritis. CD‐RAP concentrations were measured using a human CD‐RAP ELISA. Intra‐ and interassay imprecision of the assay were evaluated by multiple measurements on pools of equine synovial fluid. Assay inaccuracy was determined by linearity under dilution. Results: The assay showed moderate to large intra‐ and interassay variation when applied to equine synovial fluid. Equine CD‐RAP was detected in synovial fluid from healthy horses ranged at 8.2–52 ng/ml. Repeated arthrocentesis (after injection of isotonic saline), age, joint or gender did not significantly affect CD‐RAP concentrations. Twelve hours after intra‐articular injection of lipopolysaccharide, concentrations of CD‐RAP were significantly lower than after injection of isotonic saline and remained significantly lower until the end of the study at 144 h. Conclusion and potential relevance: The assay is suitable for longitudinal monitoring of CD‐RAP concentration in individual horses. Disease significantly influenced CD‐RAP levels. Similar to previous results obtained in man, CD‐RAP seems to be a marker of cartilage synthesis and/or regeneration in horses.  相似文献   

新生犊牛关节炎诊断与防治的研究进展          下载免费PDF全文

张雨  洛松西热  李家奎 《中国牛业科学》2023,49(4):57-60

新生犊牛关节炎是由大肠杆菌、化脓隐秘杆菌和支原体等病原微生物感染引起的以多发性关节炎为特征的疾病。该病好发于新生犊牛,成年牛偶有发病,淘汰率高,严重影响养奶牛业的健康发展。本文就犊牛关节炎的流行病学、临床症状、病理变化、诊断方法及防治措施等方面的最新研究进展做一概述,旨在为新生犊牛关节炎的诊断与防治提供参考。  相似文献   

厚壳贻贝脂溶性提取物抗炎生物活性研究   总被引：6，自引：0，他引：6       下载免费PDF全文

袁高峰  姚婷  励炯  张治国  李铎 《浙江大学学报(农业与生命科学版)》2007,33(2):169-173

    以我国海洋养殖的主要贻贝品种之一厚壳贻贝为材料,通过大鼠棉球肉芽肿和佐剂诱导的关节炎模型研究厚壳贻贝脂溶性提取物的抗炎生物活性.结果表明厚壳贻贝脂溶性提取物具有很强的抗炎生物活性,能显著抑制大鼠棉球肉芽肿和大鼠佐剂性关节炎原发性和继发性症状,其抗炎生物活性比新西兰绿唇贻贝脂溶性提取物稍低或与之相当.这为进一步把贻贝作为具有抗炎保健功能的食品或具有自主知识产权的纯天然安全有效的新型抗炎药物开发利用提供了理论基础和依据.  相似文献   

中医药治疗急性痛风性关节炎用药规律分析          下载免费PDF全文

饶春梅  任婷  成细华  黄政德 《勤云标准版测试》2017,37(5):552-555

目的 探讨中医药治疗急性痛风性关节炎的用药规律,以指导临床选方用药。方法 通过以“急性痛风性关节炎”为主题词,“中医药治疗”“中医治疗”为副主题词,检索“中文期刊数据库CNKI”2010-2015年的相关医学文献,共搜索文献216篇,其中符合条件的139篇,将纳入文献中所包括的单味中药输入Excel 2003进行一般统计学分析。结果 139篇文献中,共涉及中药复方147首,药物134味,用药频次共达1 501次,其中,用药频次前10位的药物分别是：黄柏、牛膝、薏苡仁、苍术、土茯苓、泽泻、萆薢、防己、甘草、威灵仙;药物所属类别最多的分别是：清热药、活血化瘀药、祛风湿药、补虚药、利水渗湿药。结论 清热利湿、活血通络、泄浊解毒、兼顾补虚对急性痛风性关节炎具有较好疗效。  相似文献   

湖南地区类风湿关节炎中医证型与临床检测指标关系研究          下载免费PDF全文

邝涛  李鑫  王宝新  魏艳霞  王莘智  李如意  余黄合  宋厚盼  范伏元  刘平安  黄惠勇  蔡雄 《勤云标准版测试》2017,37(7):745-748

目的 探索湖南地区类风湿关节炎（Rheumatoid arthritis,RA）中医证型与临床检测指标的关系,为RA临床辨证论治提供参考依据。方法 采集2015年1月-2016年5月于湖南中医药大学第一附属医院风湿科就诊且确诊为RA病历资料299例。采用Epidata 3.1建立数据库,并采用SPSS 22.0软件进行数据处理,分析RA中医证型与类风湿因子、血沉、C反应蛋白、肝功能等临床检测指标关系。结果 血沉：湿热痹阻证血沉表达水平与肝肾阴虚证比较,差异有统计学意义（P=0.021）。类风湿因子：各证型间的类风湿因子表达水平比较,差异无统计学意义（P>0.05）。C反应蛋白：湿热痹阻证表达水平与寒湿痹阻证比较,差异具有统计学意义（P=0.022）。肝功能：RA各证型球蛋白平均表达量均高于正常值,白球比均低于正常值,但各证型间比较,差异无统计学意义（P>0.05）。结论 RA中医证型与临床检测指标存在一定关系,血沉是潜在的湿热痹阻证与肝肾阴虚证辨证指标,C反应蛋白是潜在的湿热痹阻证与寒湿痹阻证辨证指标,但其临床检验效能尚有待于进一步研究。  相似文献   

仔猪关节炎粪肠球菌生物学特性研究     

王亚宾  张祥  胡清林  孟振北  陈丽颖  陈小丽  崔保安 《河南农业大学学报》2011,45(2):183-187

从病猪肿大的跗关节中分离了1株疑似肠球菌菌株,经过常规方法进行染色特性、培养特性观察以及敏感性和致病试验,利用Vitek-32全自动细菌鉴定系统进行生化特定,并用PCR方法扩增分离株的16S rRNA基因克隆测序,与GenBank上登录的相关菌株及3个粪肠球菌标准菌株进行16S rRNA序列比较、同源性分析并构建系统发...  相似文献   

Epidemiological and genetical studies in Norwegian pig herds. V. Estimates of heritability and phenotypic correlations of the most common diseases in Norwegian pigs     

Frode Lingaas  Knut Rnningen 《Acta veterinaria Scandinavica》1991,32(1):115-122

A genetic analysis was performed on disease registrations from 70 Norwegian pig herds. This analysis was complicated by the small degree of cross classification of dams and sires across herds, and the relatively sparse use of artificial insemination. Herd effects seemed to be responsible for much of the variation in disease frequencies. The estimated heritabilities were relatively low. The results of this investigation also revealed positive phenotypic correlations between the investigated diseases.  相似文献   

佐剂性关节炎大鼠成纤维样滑膜细胞的体外培养与鉴定     

毛伟  李培锋  刘明强  关红 《动物医学进展》2011,32(3):78-82

探讨佐剂性关节炎(AA)大鼠成纤维样滑膜细胞(FLS)的体外培养及鉴定方法,制备AA大鼠模型,采用组织块培养法培养AA大鼠FLS,并通过形态学、透射电镜及RT-PCR方法对FLS进行鉴定.结果表明,培养的滑膜细胞具有成纤维细胞的形态和特征,呈长梭形板向生长,RT-PCR方法检测波形蛋白表达较高.本研究成功地培养出了AA...  相似文献   

Effects of (+)-2-(1-hydroxyl-4-oxocyclohexyl)ethyl caffeate at different doses on rat arthritis model and whole blood inflammation model     

YANG Wen  LIU Yuan-ru  LI Hong-lin  WANG Rui 《园艺学报》2018,34(12):2247-2254

AIM:To evaluate the pharmacodynamics of (+)-2-(1-hydroxyl-4-oxocyclohexyl)ethyl caffeate (HOEC), and to explore the possible causes of non-dose-dependent effects of HOEC on collagen-induced arthritis (CIA) rats using the arachidonic acid (AA) metabolic model in rat whole blood. METHODS:The rat CIA model was used to study the treatment with HOEC at 3 doses. The expression of cytoplasmic phospholipase A₂ (cPLA₂), 5-lipoxygenase (5-LOX) and cyclo-oxygenase-2 (COX-2) was assayed by immunohistochemical method. The effects of HOEC and its in vivo metabolite caffeic acid (CA) on AA metabolite in rat whole blood were measured by ELISA. RESULTS:HOEC had a therapeutic effect on rat CIA, but the curative effect at low dose and middle dose (1 and 3 mg/kg) was better than that at high dose (10 mg/kg). The expression levels of cPLA₂, 5-LOX and COX-2 in joint tissues were decreased. HOEC inhibited the metabolites of LOX and COX pathways in the rat whole blood AA metabolic model, while the inhibitory effect of CA on these metabolites was weaker than that of HOEC. CONCLUSION:The anti-inflammatory effect of HOEC on rat CIA may be associated with the inhibition of cPLA₂, 5-LOX and COX-2 expression in the joint tissues. The non-dose-dependent therapeutic effect of HOEC on rat CIA may due to the weaker inhibitory activity of CA on AA metabolic model in rat whole blood than that of HOEC.  相似文献   

The Norwegian Healthier Goats programme – A financial cost–benefit analysis     

G. Elise Nagel-Alne  Leif J. Asheim  J. Brian Hardaker  Liv Sølverød  Dag Lindheim  Paul S. Valle 《Preventive veterinary medicine》2014

The aim of this study was to evaluate the profitability to dairy goat farmers of participating in the Healthier Goats disease control and eradication programme (HG), which was initiated in 2001 and is still running. HG includes the control and eradication of caprine arthritis encephalitis (CAE), caseous lymphadenitis (CLA) and paratuberculosis (Johne's disease) in Norwegian goat herds.  相似文献