猪繁殖与呼吸综合征疫苗研究现状   总被引：2，自引：0，他引：2  

沈武玲  乔自林  令世鑫  冯玉萍  冯若飞  李明生  林学仕  柏家林  王家敏  马忠仁 《动物医学进展》2012,(1):117-120

猪繁殖与呼吸综合征(PRRS)是一种对养猪业危害严重的传染病,猪繁殖与呼吸综合征病毒(PRRSV)为有囊膜的单股正链RNA病毒,且具有高度的变异性,有美洲型和欧洲型两个血清型。猪肺泡巨噬细胞(PAM)、恒河猴胎肾细胞系MA-104及源于MA-104的传代细胞系(如Marc-145、CL-2621、HS.2H和CRL-1171)对PRRSV敏感。预防该病的常规疫苗有灭活疫苗和弱毒疫苗两种。通常以特定种毒在适当细胞系中培养增殖,经灭活或致弱,加入佐剂制得相应疫苗。灭活疫苗交叉保护性差,但使用安全;弱毒疫苗抗体产生快、持续时间长、保护力强,但源毒能在猪群中持续存在而有安全隐患。  相似文献   

以减毒鼠伤寒沙门氏菌运送的H5亚型禽流感病毒DNA疫苗的安全性与免疫效力   总被引：3，自引：0，他引：3  

张小荣  焦新安  潘志明  张晓明  黄金林  张如宽  刘秀梵 《中国兽医学报》2004,24(6):531-533

将运送 H5亚型高致病力禽流感病毒 DNA疫苗的重组减毒鼠伤寒沙门氏菌 X4 5 5 0 (p VAX1- HA)和 X4 5 5 0 (asd-p VAX1- HA )以 1× 10 1 0 CFU的剂量腹腔注射 1日龄 SPF白莱航雏鸡 ,结果表明 ,二者均具有良好的安全性。对 SPF白莱航鸡的免疫原性试验结果表明 ,这 2种细菌均能刺激鸡体产生高水平的肠道粘膜免疫应答 ,但是不能有效地激发血清抗体应答。对商品代伊莎褐蛋鸡的免疫效力试验初步结果显示 ,X4 5 5 0 (asd- p VAX1- HA)免疫鸡能够抵抗强毒的攻击 ,保护指数为 77.8% ,而 X4 5 5 0 (p VAX1- HA)则未产生免疫保护  相似文献   

EnMIC2重组减毒沙门氏菌的构建及免疫保护效果研究   总被引：5，自引：4，他引：5  

谢明权  覃宗华  蔡建平  艾哈迈德  彭新宇  魏文康  吴惠贤 《畜牧兽医学报》2005,36(7):705-710

成功构建了表达鸡毒害艾美耳球虫(Eimeria necatrix)广东株(GD)微线蛋白-2基因(EnMIC2)的重组减毒鼠伤寒沙门氏菌，在IPTG诱导下获得了EnMIC2的高效表达，SDS-PAGE分析表明，表达产物约占菌体总蛋白的8．6％，分子量大小约为35ku，与抗E．necatrix的高免血清进行Western Blotting，结果为阳性。将重组减毒沙门氏菌以10^8和10^9 CFU／鸡免疫4日龄岭南黄肉鸡，免疫后2周血清中可检测到特异性IgG，3周时抗体水平达到峰值，同时可在肠道检测到特异性IgA的分泌。免疫接种后3周，试验鸡分别经口攻击感染500个E．necatrix(GD)孢子化卵囊，结果显示免疫组与非免疫组有相似的排卵囊曲线；但10^8和10^9CFU免疫组的卵囊总产量分别能降低26．62％和48．92％。  相似文献   

鸭瘟病毒弱毒株在免疫雏鸭体内的分布和排毒规律   总被引：9，自引：5，他引：9  

程安春  汪铭书  刘菲  宋涌  袁桂萍  韩晓英  徐超  廖永洪  文明  周伟光  贾仁勇 《中国兽医学报》2005,25(3):231-233,258

鸭瘟病毒(DPV)弱毒Cha株经皮下、口服和滴鼻3种途径免疫1日龄雏鸭，应用聚合酶链反应(PCR)检测了病毒在体内分布和排毒规律。Cha株免疫雏鸭后，对血液、心、肝、脾、肺、肾、十二指肠、直肠、法氏囊、胸腺、胰腺、延脑、大脑、小脑、舌、肌肉、骨髓、粪便和食道共19种组织PCR检测结果如下：(1)皮下接种雏鸭后4h，即可在心、肝、脾、肾、法氏囊、胸腺、胰腺、延脑、大脑和小脑共10种组织中检出DPV的DNA；8h后，所有采取的组织器官均可检测到DPV的DNA。(2)口服接种雏鸭后4h，可在舌和食道中检测到DPV的DNA；8h后，可在心、肝、脾、肾、胸腺、胰腺、延脑、大脑、小脑、舌、食道和血液共12种组织器官中检出DPV的DNA。(3)滴鼻接种雏鸭后4h，未能在各种组织中检出DPV的DNA；8h后，可在心、肝、脾、肾、胸腺、延脑、大脑、小脑、舌、食道和血液共11种组织中检测到DPV的DNA。(4)在3种免疫途径中，检出时间最早和检出率最高的组织器官为肝脏、脑(大脑、小脑和延脑)；3种途径免疫的鸭，从免疫后12h至21d均能从所有采集的组织中检测出DPV DNA。  相似文献   

中国马传染性贫血病毒DLV株前病毒gp90基因在马体内的变异分析     

杨彬  王雪峰  周建华  王凤龙 《动物医学进展》2008,29(11)

分别以EIAV辽宁马强毒株（EIAVliao）前病毒DNA和马传染性贫血驴白细胞弱毒疫苗株（EIAVDLV）前病毒DNA为模板,从免疫接种后不同时期马血清中利用nested—PCR技术,扩增了约1．4kb的gp90基因。将其克隆后进行了测序,测序结果表明,免疫EIAVDLV后的第1时期至第5时期,与EIAV—liao比较核苷酸序列平均差异率分别为3．2956％、3．1456oA、3．36％、3．1856％和3．6456％。EIAVliao有20个N-连接糖基化位点,EIAVDLV平均是17．2个,其中有11个N-连接糖基化位点是高度保守的。  相似文献   

小鼠对pGM-CSF/SS生长抑素基因疫苗的免疫应答     

梁爱心  姚艳丰  金定恩  武睿  韩丽  付平  刘耘  杨利国 《华中农业大学学报》2006,25(5):540-543

通过研究细胞因子GM-CSF与生长抑素pcS/2SS融合表达质粒pGM-CSF/SS诱导小鼠免疫应答的影响,探求生长抑素基因免疫新策略。将60只20日龄雄性昆明小白鼠均分为6组,分别用PBS(C0)、pGM-CSF质粒(C1)、pGM-CSF/SS质粒(T1、T2、T3)、pcS/2SS(T4)质粒口服免疫,T1~T4组的免疫剂量分别为2×106cfu/mL、2×108cfu/mL、2×1010cfu/mL、2×108cfu/mL,以上质粒均以减毒沙门氏菌为传导载体。结果显示:pGM-CSF/SS组的抗生长抑素抗体AP/AN值显著高于pcS/2SS组,阳性鼠体增重亦高于pcS/2SS组,抗体阳性率分别是71.4%和40.0%。另外,高剂量组的AP/AN值极显著高于低剂量组,显著高于中剂量组;中剂量组的AP/AN值显著高于低剂量组,高剂量组的抗体阳性率也明显高于中剂量组和低剂量组,分别为100%、71.4%和20.0%。可见,pGM-CSF/SS疫苗的免疫效果优于pcS/2SS疫苗,且pGM-CSF/SS疫苗呈剂量依赖关系。这些结果显示出细胞因子GM-CSF可以增强生长抑素DNA疫苗的免疫效果。  相似文献   

家兔免疫伊氏锥虫弱毒疫苗后的T细胞,IgG和IgM动态变化     

王景林  陈启军 《沈阳农业大学学报》1999,30(2):134-136

分别以3Ｈ—ＴｄＲ掺入的淋巴细胞转化反应和ＢＡ—ＥＬＩＳＡ方法检测家兔免疫后及攻虫后Ｔ细胞及特异性抗体ＩｇＧ和ＩｇＭ的动态变化。发现免疫后家兔外周血Ｔ淋巴细胞增殖反应于２４ｄ达到高峰,第３１天开始迅速降低。攻虫后特异性Ｔ细胞增殖反应强度未见升高,而特异性抗体Ｉｇｈ、ＩｇＭ均于第２１天达到高峰,攻虫后ＩｇＧ、ＩｇＭ分别在第６天和第９天又达到高峰。结果表明,在这种抗锥虫免疫中Ｔ细胞没有起主导作用,而特异性抗体ＩｇＧ和ＩｇＭ起主导作用。  相似文献   

Cross-protection of a live-attenuated Flavobacterium psychrophilum immersion vaccine against novel Flavobacterium spp. and Chryseobacterium spp. strains     

Timothy J. Bruce  Jie Ma  Christopher Knupp  Thomas P. Loch  Mohamed Faisal  Kenneth D. Cain 《Journal of fish diseases》2020,43(8):915-928

For salmonid producers, a common threat is Flavobacterium psychrophilum. Recent advancements in bacterial coldwater disease (BCWD) management include the development of a live-attenuated immersion vaccine that cross-protects against an array of F. psychrophilum strains. Emerging family Flavobacteriaceae cases associated with clinical disease have been increasing, including pathogenic isolates of Flavobacterium spp. and Chryseobacterium spp. The cross-protective ability of a live-attenuated F. psychrophilum vaccine was determined against three virulent Flavobacteriaceae isolates. Juvenile rainbow trout were vaccinated, developed high F. psychrophilum-specific antibody titres and were challenged with Chryseobacterium spp. isolates (S25 and T28), a Flavobacterium sp. (S21) isolate, a mixed combination of S21:S25:T28, and a standard virulent F. psychrophilum CSF259-93 strain. Results demonstrated strong protection in the CSF259-93 vaccinated group (relative per cent survival (RPS)=94.44%) when compared to the relevant CSF259-93 controls (p < .001). Protection was also observed for vaccinated fish challenged with the S21:S25:T28 mix (RPS = 85.18%; p < .001). However, protection was not observed with the S21, S25 or T28 isolates alone. Analysis of whole-cell lysates revealed differences in protein banding by SDS-PAGE, but conserved antigenic regions by Western blot in S25 and T28. Results demonstrate that this live-attenuated vaccine provided protection against mixed flavobacterial infection and suggest further benefits against flavobacteriosis.  相似文献   

番木瓜环斑病毒西瓜株系弱毒突变体的筛选与应用     

黄显德  王玉  闫志勇  耿超  田延平  李向东 《植物保护学报》2019,46(4):738-744

为防治番木瓜环斑病毒(Papaya ringspot virus,PRSV)在我国葫芦科作物上引起的病毒病害,以PRSV西瓜株系山东分离物(PRSV-SD)侵染性cDNA克隆为基础,采用定点突变方法将辅助成分-蛋白酶保守氨基酸137位和346位的天冬酰胺(N)和417位的缬氨酸(V)突变为丙氨酸(A),应用农杆菌浸润法接种西葫芦叶片并分析突变对PRSV-SD致病力的影响,筛选弱毒突变体,进而评价其交叉保护效果。结果表明,与野生型PRSV-SD相比,获得的3个突变体N137A、N346A和V417A,接种后在西葫芦植株上的症状明显减轻,衣壳蛋白在叶片中的积累水平分别为野生型PRSV-SD的24.0%、13.0%和4.0%,均为弱毒突变体。当保护间隔期为10 d时,弱毒突变体N137A具有完全的交叉保护效果,N346A可延迟发病15 d,而V417A无交叉保护效果。当间隔保护期为15 d时,弱毒突变体N137A和N346A的保护效率分别为100.0%和26.7%,而V417A无交叉保护效果。  相似文献   

Optimization of Cryoprotectant for Attenuated Salmonella Cholerae C500     

YANG Shuai  ZHOU Di  ZHANG Xinxin  HU Xiangwei  YANG Liguo 《中国畜牧兽医》2007,47(11):3696-3704

The purpose of this study was to optimize the antifreeze protectant of attenuated Salmonella Cholerae C500 and improve the survival rate of C500.The effect of four types of cryoprotectant (permeating,semi-permeating,non-permeating cryoprotectants and antioxidants) on the survival rate of C500 before and after cryopreservation was investigated by single factor test.Each cryoprotectant was set at different concentrations and mixed in equal volume of C500.After two weeks of cryopreservation in liquid nitrogen,becteria plate count was carried out to calculate the survival rate,and the best one of the permeating cryoprotectant,semi-permeating cryoprotectant,non-permeating cryoprotectant and antioxidants and their optimal concentrations were beneficial to the preservation of bacteria was initially selected.Finally,the four protectors selected were tested by orthogonal test of four factors and three levels to get the optimal combination formula.The results of single factor test showed that the best permeating,semi-permeating,non-permeating cryoprotectants and antioxidants were glycol,sucrose,PVP and glycine,and the corresponding preservation concentrations were 15%,6%,6% and 0.25%,respectively.The orthogonal test showed that each factor had a significant impact on the survival rate of C500.The optimal combination formula was 17% glycol,9.0% sucrose,6.0% PVP and 0.35% glycine.The optimal combination had the optimal effect at -80 ℃,the survival rate was 88.34% in 6 weeks,followed by liquid nitrogen group,-20 ℃ group and 4 ℃ group.The results showed that the formulation of the protective agent obtained in this experiment greatly improved the antifreeze ability of C500 and its survival rate.  相似文献