非洲猪瘟病毒诱导猪红细胞凋亡并促进猪外周血单核细胞的吞噬作用     

杨云龙  冯永智  高琦  郑佳琛  王衡  张桂红  龚浪 《畜牧兽医学报》2022,53(11):3948-3955

本研究旨在探讨非洲猪瘟病毒（ASFV）对猪红细胞（RBCs）的作用以及对猪外周血单核细胞（PBMs）吞噬能力的影响。试验采用流式细胞术检测ASFV侵染猪原代肺泡巨噬细胞（PAMs）和猪红细胞（RBCs）的能力,并检测ASFV诱导RBCs发生凋亡的百分比;同时采用激光共聚焦试验（Confocal）观察ASFV诱导RBCs发生凋亡是否影响PBMs的吞噬能力。结果显示,ASFV不能入侵RBCs,但以时间和剂量依赖性方式诱导RBCs发生凋亡。0.1 MOI ASFV接种RBCs后1、3、5和7 d可分别诱导1.27%、3.23%、7.39%和8.56%的RBCs发生凋亡;1 MOI ASFV接种RBCs后1、3、5和7 d可分别诱导1.54%、3.73%、8.46%和10.74%的RBCs发生凋亡;3 MOI ASFV接种RBCs后1、3、5和7 d可分别诱导2.65%、5.01%、12.44%和18.61%的RBCs发生凋亡。同时,凋亡的RBCs可以增加PBMs对黄绿色荧光微球的吞噬数量,ASFV诱导RBCs凋亡的百分比越高,PBMs吞噬黄绿色荧光微球的数量越多。综上所述,ASFV不能侵染猪RBCs,但可以以时间和剂量依赖性方式诱导RBCs发生凋亡并增强PBMs的吞噬功能。  相似文献   

白头翁及皂苷对鸡免疫功能的影响   总被引：1，自引：0，他引：1  

时维静 《中国兽药杂志》2009,43(7):13-15

为考察白头翁及白头翁皂苷对鸡免疫功能的影响,将AA肉鸡于15日龄添加白头翁及白头翁皂苷饮水,以黄芪组为阳性对照、未加药为阴性对照。通过测定免疫器官增重、白细胞吞噬率与吞噬指数等,考察白头翁及白头翁皂苷对鸡免疫功能的影响。结果表明：白头翁及其皂苷组比对照组在体重和免疫指数方面有明显的优越性,尤其在中性细胞吞噬白色葡萄球菌方面与对照组有极显著差异（P〈0.01）。白头翁及白头翁皂苷对鸡免疫有一定的促进作用,试验为白头翁生产应用提供了参考数据。  相似文献   

黄芪多糖饲料添加剂对罗非鱼白细胞吞噬能力和免疫器官指数的影响   总被引：1，自引：0，他引：1  

林旋  张伟妮  王寿昆  黄文焱  陈佳铭  赵堇 《福建农业学报》2012,(1):27-31

选用罗非鱼120尾,随机分为5组。对照组饲喂基础日粮,试验组分别饲喂在基础饲料中添加100、500、1 000、1 500mg.kg-1的黄芪多糖试验日粮,投喂40d。每10d检测白细胞的吞噬能力和免疫器官增重情况。结果显示:500mg.kg-1、1 000mg.kg-1和1 500mg.kg-1组均能显著提高罗非鱼血液中白细胞吞噬能力,增加免疫器官的重量(P<0.05);而添加剂量为100mg.kg-1则不明显(P>0.05)。表明黄芪多糖可以不同程度地提高罗非鱼血液中白细胞的吞噬能力,促进免疫器官的发育与成熟。  相似文献   

Phagocytic Uptake of Nosema bombycis （Microsporidia） Spores by Insect Cell Lines     

CAI  Shun-feng LU  Xing-meng QIU  Hai-hong LI  Ming-qian FENG  Zhen-zhen 《中国农业科学(英文版)》2012,11(8):1321-1326

  相似文献   

Influence of Dexamethasone on Some Cellular Aspects of the Immune System in Cats     

Hoffmann-Jagielska M  Winnicka A  Jagielski D  Lechowski R 《Veterinary research communications》2003,27(8):643-652

In view of the frequent use of glucocorticoids in the treatment of cats, we studied the effect of dexamethasone on their immunological system. The phagocytic activity and oxidative burst of neutrophils and monocytes were evaluated by cytometric analysis using commercial kits and the subpopulations of lymphocytes were assessed. Neutrophilia and monocytosis reduced phagocytic activity, as shown from the number of phagocytized bacteria, and variations in the intensity of the oxidative burst in activated neutrophils and monocytes were observed. Dexamethasone also caused an increase in the number of B lymphocytes.  相似文献   

Studies on Some Aspects of Neutrophil Functions and Uterine Defenses in Cows during the Estrous Cycle     

A.M. Hussain  R.C.W. Daniel 《Reproduction in domestic animals》1991,26(6):290-296

Some aspects of the bovine uterine defense mechanisms were evaluated over two successive estrous cycles in five adult nonpregnant Friesian cows. Uterine flushings and blood samples were taken from each cow during the estrus and diestrus phases of each estrous cycle. A significant higher percentage of Candida albicans (C. albicans) were phagocytosed (P < 0.01) and killed (P < 0.05) by blood neutrophils during estrus than during diestrus. N-acetyl-β-D-glucosaminidase activity of uterine flushings, blood neutrophil alkaline phosphatase activity scores, and serum progesterone concentrations during diestrus increased significantly (P < 0.01) compared to those at estrus. Mean total blood leucocyte and monocyte counts increased significantly (P < 0.05) during estrus compared to diestrus .
It is concluded, that the different hormonal status has at each stage of the estrous cycle a definite effect on uterine resistance against bacterial infection and a fall in serum progesterone concentrations is associated with an increased ability of blood neutrophils to phagocytose and kill C. albicans .  相似文献   

Effects of Enolase of Streptococcus equi ssp. zooepidemicus on Phagocytic Functions of Alveolar Macrophages in Mice     

CHEN Kaiwen  HUA Chengwei  YUAN Chen  PAN Fei  LIN Huixing  FAN Hongjie  MA Zhe 《畜牧兽医学报》1956,51(10):2576-2583

To investigate the effect of enolase (Eno) of Streptococcus equi ssp. zooepidemicus (SEZ) on phagocytosis of mouse alveolar macrophages (RAW264.7). Recombinant enolase (rEno) was obtained by constructing prokaryotic expression plasmid, and the cytotoxicity of rEno protein on RAW264.7 cell proliferation was determined by trypan-blue living cell count method. After the rEno protein was incubated with RAW264.7 cells, SEZ was applied to the cells and the quantity of bacteria being phagocytosed was detected to determine the phagocytic activity of RAW264.7 cells. Further, candidate proteins that might interact with SEZ Eno in RAW264.7 cells were screened by live cell stable isotope labeling (SILAC) and protein spectrum analysis (LC-MS/MS). It was found that protein treatment (rEno,10 μg·mL^-1) had significant cytotoxic effects on RAW264.7 cells. Treatment of RAW264.7 cells with 10.0 μg·mL^-1 rEno protein for 2 and 4 hours could significantly inhibit the phagocytosis of RAW264.7 cells (P<0.01, P<0.05). In RAW264.7 cells, dynactin subunit protein 2 (Dctn), integrin alpha-M and about 17 proteins that might interact with Eno were preliminarily identified as rEno interaction proteins. The rEno recombinant expression protein was obtained in this study, and it could reduce the phagocytosis of RAW264.7 cells to SEZ. Preliminary screening of interacting proteins also laid a foundation for further revealing the mechanism of Eno in the anti-phagocytosis of SEZ.  相似文献   

牛多形核白细胞(PMN)中P38 MAPK,PKC和PI3-K介导的NADPH氧化酶激活和吞噬作用(英文)     

崔玉东 《中国预防兽医学报》2000,(Z1)

用血清调理的酵母聚糖（ｓｅｒｕｍ－ｏｐｓｏｎｉｚｅｄ　ｚｙｍｏｓａｎ　,ｓＯＺ）刺激多性核白细胞（ＰＭＮ）引起的Ｏ２－产生受到ｐ３８ＭＡＰＫ抑制物（ＳＢ２０３５８０）,ＰＩ３－Ｋ抑制物（ｗｏｒｔｍａｎｎｉｎ）和ＰＫＣ抑制物（ＧＦ１０９２０３Ｘ）的明显抑制,这些抑制物也明显引起ｓＯＺ诱导的一种ＮＡＤＰＨ氧化酶的胞浆成分 ｐ４７ｐｈｏｘ的磷酸化。可是流式细胞分析表明,ＳＢ２０３５８０和 ｗｏｒｔｍａｎｎｉｎ使吞噬作用减弱,而 ＧＦＩＯ９２０３Ｘ使吞噬作用增强。这些结果表明,虽然ＰＩ３－Ｋ和ｐ３８ ＭＡＰＫ都参与ＮＡＤＰＨ氧化酶激活和吞噬作用的信号传导,但ＮＡＤＰＨ氧化酶激活的信号传导途径与吞噬作用的信号传导途径不同。  相似文献   

旋毛虫重组二肽基肽酶1在体外对大鼠外周血单个核细胞增殖、吞噬、凋亡等的影响     

廖书漪  储稳  徐立新  宋小凯  李祥瑞  严若峰 《畜牧兽医学报》2020,51(6):1362-1370

本文旨在分析旋毛虫二肽基肽酶1（dipeptidyl-peptidase 1，Dpp1）在体外对大鼠外周血单个核细胞（peripheral blood mononuclear cells，PBMCs）增殖、迁移、凋亡以及一氧化氮（NO）分泌和吞噬功能的影响。根据GenBank中旋毛虫Dpp1基因序列设计一对特异性引物，RT-PCR获得该基因，将其亚克隆到pET-32a原核表达载体，IPTG诱导后获得重组蛋白rDpp1。将大鼠PBMCs与rDpp1共孵育，用间接免疫荧光试验（IFA）分析rDpp1与大鼠PBMCs的结合情况，分析不同质量浓度（10、20、40 μg·mL^-1）的rDpp1对细胞增殖、迁移、NO分泌、吞噬功能和细胞凋亡的影响。结果表明，40 μg·mL^-1rDpp1极显著促进PBMCs增殖；10 μg·mL^-1rDpp1能显著促进PBMCs迁移，而20及40 μg·mL^-1时表现极显著的促进；20与40 μg·mL^-1rDpp1极显著促进PBMCs分泌NO；10 μg·mL^-1rDpp1能显著促进PBMCs的吞噬功能，而20及40 μg·mL^-1时表现极显著的促进；各质量浓度蛋白均极显著地促进PBMCs的凋亡。旋毛虫二肽基肽酶1在体外能通过多种途径影响大鼠外周血单个核细胞，对调节宿主免疫应答有一定的促进作用。  相似文献   

温度对草鱼血液吞噬细胞吞噬作用的影响   总被引：7，自引：0，他引：7  

孟跃华  纪国良 《华中农业大学学报》1990,9(3):302-305

  相似文献