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排序方式: 共有320条查询结果,搜索用时 290 毫秒
1.
Seasonal Changes in the Milt Quality of Waigieu Seaperch,Psammoperca waigiensis: Implications for Artificial Propagation
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Milt quality of Waigieu seaperch was sampled at the beginning, the middle, and the end of the spawning season to assess if the sampling season has an effect on milt quality parameters. The milt volume and total sperm production were higher at the middle of the spawning season while the sperm concentration in milt was significantly higher at the beginning and the end of the spawning season. Sperm morphology and the major parameters of seminal plasma (pH, total protein, Mg2+, or Ca2+ concentrations) did not differ throughout the spawning season. The concentrations of Na+ and the osmolality increased throughout the spawning season. The percentage of motile cells did not differ during the spawning season while the duration of sperm motility (swimming duration) and velocity (swimming speed) were significantly different during the spawning season. The fertilization rate (75.7 ± 6.8%) and hatching rate (56.5 ± 3.1%) at the middle of the spawning season were higher than at the beginning and end periods. Results indicated that milt quality parameters and fertility success in the middle of the spawning season were higher compared to earlier and later sampling dates. Thus, we recommend collecting milt during this time for maximal fertilization and hatching success. 相似文献
2.
AbstractThe effect of different rice-based crop rotation sequences on the population densities of Meloidogyne graminicola and on the yield of rice was evaluated in a microplot experiment under lowland rice field conditions. Ten treatments of cropping sequences were grown in four successive growing seasons: continuous growing of the susceptible rice cultivar Thihtatyin (rice-rice-rice-rice), four treatments of 1- season crop rotation sequences (rice-rice-chickpea-rice, rice-rice-black gram-rice, rice-rice-soybean-rice, rice-rice-cowpea-rice), and five treatments of 2-season crop rotation sequences (groundnut-rice-chickpea-rice, green gram-rice-black gram-rice, cowpea-rice-soybean-rice, sesame-rice-cowpea-rice, sunflower-rice-sesame-rice). Population densities of M. graminicola recovered from the rice plants and the nematode multiplication factors (Mf) in the soil in the continuous rice cropping sequence (rice-rice-rice-rice) was the highest among the ten cropping sequences. Lowest nematode Mf in the soil was observed in the 2-season crop rotation sequence sunflower-rice-sesame-rice and the highest was observed in the 1-season crop rotation sequence rice-rice-cowpea-rice among the nine crop rotation sequences. Highest rice yield was obtained in the 2-season crop rotation sequences green gram-rice-black gram-rice, sesame-rice-cowpea-rice and sunflower-rice-sesame-rice, which were about 2 times higher compared with the 1-season crop rotation sequences and about 3 times higher compared with the continuous rice cropping sequence. 相似文献
3.
Thi Thanh Nhan Pham Thi Ngoc Lan Nguyen Thi Ha Bui Huu Quan Nguyen Thi Tam Nguyen Van Son Le 《The Journal of Horticultural Science and Biotechnology》2019,94(5):591-598
Catharanthus roseus contains vincristine and vinblastine, which are outstanding drugs for cancer. In the biosynthetic pathways of terpenoid indole alkaloids (TIAs) in C. roseus, deacetylvindoline 4-O-acetyltransferase (DAT) is a key enzyme that catalyses the last reaction of vindoline biosynthesis to form vinblastine and vincristine. In this study, the CrDAT transgene was transferred into the periwinkle by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The C. roseus DAT gene was introduced into C. roseus plants and it was confirmed that CrDAT was successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein. Four transgenic periwinkle lines in T1 generation, T1-1, T1-3, T1-6, and T1-7, expressed recombinant DAT protein with the total protein content in the range of 2.86 μg.mg?1 to 5.12 μg.mg?1. Moreover, the vincristine contents of four transgenic lines increased by 1.63?2.48-fold compared to non-transgenic plants, ranging from 6.91 µg.g?1 (fresh weight) to 10.53 µg.g?1 (fresh weight). The T1-1 line had the highest vincristine content. Hence, the overexpression of the recombinant DAT protein can improve the vincristine accumulation of transgenic C. roseus plants.Abbreviation: CrDAT - Catharanthus roseus Deacetylvindoline-4-O-Acetyl Transferase; D4H - Deacetoxyvindoline 4-hydroxylase; ELISA - Enzyme-Linked Immunosorbent Assay Monoterpene indole alkaloid; T0, T1 - Generations of transgenic plants; TIAs - Terpenoid indole alkaloids; WT- The wild-type tobacco plants (non transgenic plant); 35S - Cauliflower mosaic virus 35S promoter 相似文献
4.
Effects of Dietary Digestible Protein and Energy Levels on Growth Performance,Feed Utilization,and Body Composition of Juvenile Permit,Trachinotus falcatus (Linnaeus, 1758)
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Huy Quang Nguyen Thiet Chi Chu Thuy Thi Le Nguyen Ivar Lund 《Journal of the World Aquaculture Society》2018,49(5):943-952
A 9‐wk study was conducted to evaluate the effect of dietary protein and energy on growth performance of juvenile permit, Trachinotus falcatus, growing from approximately 30 to 150 g. Nine experimental diets were formulated to contain three levels of crude protein (400, 450, and 500 g/kg dry matter [DM]); and three levels of crude lipid (100, 200, and 300 g/kg DM) in a 3 × 3 factorial design. Growth rate and feed efficiency were significantly improved with increasing dietary protein levels from 400 to 500 g/kg and with dietary lipid levels from 100 to 200 g/kg. Fish body protein content was positively correlated with dietary ratio of digestible protein (DP) to digestible energy (DE) (P < 0.01, R2 = 0.83), while body lipid was negatively correlated with dietary DP/DE (R2 = 0.55, P < 0.05) but positively correlated with dietary DE levels (R2 = 0.66, P < 0.01). Results showed a protein‐sparing effect, as protein retention was significantly increased by increasing dietary lipid level. In conclusion, the diet containing DP of 392.7 g/kg and DE of 18.8 MJ/kg (DM), corresponding to a DP/DE of 20.9 g/MJ, is suggested as an optimal feed for growth and feed efficiency in juvenile permit. 相似文献
5.
6.
Effects of Thyroxin and Domperidone on Oocyte Maturation and Spawning Performances in the Rabbit Fish,Siganus guttatus
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Thyroxin (T4) is proven to have various functions in fish reproduction. In this study, we investigated oocyte maturation and spawning performance in the rabbit fish, Siganus guttatus, orally exposed to different doses of T4 (0.05, 0.25, and 0.5 mg/kg fish) during vitellogenesis. No statistically significant differences of reproductive characteristics were observed among the treatments. However, the group that was exposed to the dose of 0.5 mg/kg spawned after receiving a hormone injection. The findings suggest that feeding T4 during vitellogenesis did not advance oocyte maturation, but probably could induce spawning in rabbit fish. In a separate experiment, we investigated the effects of orally administered domperidone (DOM) on reproductive performances of the female rabbit fish. The study showed that oral administration of DOM during the early stages of the reproductive cycle had no significant effects on oocyte maturation and spawning performance. Overall, these findings suggest that DOM may not be needed for the induction of maturation and spawning of this species under aquaculture conditions. These practical investigations were conducted with a view to attempt reducing drug use, production costs, and support sustainable aquaculture. 相似文献
7.
Kunimasa Kawabe Nguyen Thi Ngoc Truc Bui Thi Ngoc Lan Le Thi Thu Hong Masatoshi Onuki 《Journal of General Plant Pathology》2006,72(6):355-359
A quantification system for huanglongbing pathogen using a competitive polymerase chain reaction method and image-analyzing
software were developed to obtain precise results. Significant differences in the quantity of pathogen were thus determined
in leaves of two citrus cultivars commonly cultivated in southern Vietnam. Less pathogen-related DNA was detected from the
tissue of citrus cultivars that are believed to be more tolerant than susceptible cultivars. The quantification system will
be used in studies on pathogen proliferation and movement inside citrus tissue. 相似文献
8.
E.G. Wulff A.T.H. Pham M. Chérif P. Rey Y. Tirilly J. Hockenhull 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(1):69-76
Hydroponically grown cucumber (Cucumis sativus) seedlings were inoculated with zoospores of 1 mycoparasitic (Pythium oligandrum) and 2 pathogenic (Pythium aphanidermatum and Pythium group F) Pythium spp. During the first 2 days after inoculation, all the Pythium spp. caused reduction in the root length. However, roots treated with Pythium oligandrum quickly reached the length of the control and on the 8th day, and for the rest of the experimental period, stimulation of root elongation was noted. Pythium oligandrum was not pathogenic on cucumber and no differences in the fresh weights of control and Pythium oligandrum inoculated plants were observed in the course of the experiment. Pythium group F and Pythium aphanidermatum were pathogenic on cucumber seedlings, but their pathogenicities differed. Thus, while Pythium group F had a constant, negative influence on root length and plant growth, measured as fresh weight, Pythium aphanidermatum caused generalized necroses of the root system, inhibiting consistently root elongation and plant growth and finally causing plant death. Moreover, the zoospores of 2 mycoparasitic species, Pythium oligandrum and Pythium periplocum, were not attracted to roots of cucumber and accumulated on the roots in very low numbers compared to those of the pathogenic species, Pythium aphanidermatum, which were strongly attracted and accumulated in large numbers. Finally, it was also found that Pythium oligandrum colonized the roots very poorly, while Pythium group F and Pythium aphanidermatum were significantly better root colonizers. The significance of these findings is discussed in relation to the ecology of Pythium species and biocontrol. 相似文献
9.
A new ent-kaurane diterpenoid (1) was isolated from the leaves of Croton tonkinensis. The structure of 1 was determined as ent-7beta-hydroxy-15-oxokaur-16-en-18-ol from spectroscopic methods. 相似文献
10.
Detection of Novel Reassortant Influenza A (H3N2) and H1N1 2009 Pandemic Viruses in Swine in Hanoi,Vietnam
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T. D. Dao N. T. Pham B. J. Cowling M. Peyre M. Peiris 《Zoonoses and public health》2015,62(6):429-434
From May to September 2013, monthly samples were collected from swine in a Vietnamese slaughterhouse for influenza virus isolation and serological testing. A(H1N1)pdm09 viruses and a novel H3N2 originating from reassortment between A(H1N1)pdm09 and novel viruses of the North American triple reassortant lineage were isolated. Serological results showed low seroprevalence for the novel H3N2 virus and higher seroprevalence for A(H1N1)pdm09 viruses. In addition, serology suggested that other swine influenza viruses are also circulating in Vietnamese swine. 相似文献