全文获取类型
收费全文 | 286篇 |
免费 | 6篇 |
专业分类
林业 | 27篇 |
农学 | 10篇 |
基础科学 | 1篇 |
56篇 | |
综合类 | 24篇 |
农作物 | 17篇 |
水产渔业 | 41篇 |
畜牧兽医 | 88篇 |
园艺 | 8篇 |
植物保护 | 20篇 |
出版年
2023年 | 1篇 |
2022年 | 7篇 |
2021年 | 15篇 |
2020年 | 16篇 |
2019年 | 17篇 |
2018年 | 10篇 |
2017年 | 8篇 |
2016年 | 14篇 |
2015年 | 6篇 |
2014年 | 15篇 |
2013年 | 23篇 |
2012年 | 14篇 |
2011年 | 28篇 |
2010年 | 11篇 |
2009年 | 10篇 |
2008年 | 16篇 |
2007年 | 12篇 |
2006年 | 5篇 |
2005年 | 14篇 |
2004年 | 9篇 |
2003年 | 3篇 |
2002年 | 4篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1986年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1970年 | 1篇 |
1968年 | 1篇 |
1967年 | 2篇 |
1966年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有292条查询结果,搜索用时 46 毫秒
1.
Kasumi Sudo Mariko Ochiai Naoyuki Aihara Noriyuki Horiuchi Atsushi Yamamoto Sachiko Matsumoto Koji Oishi 《Journal of toxicologic pathology》2021,34(2):137
Batch safety tests (BSTs) of veterinary vaccines are conducted using small laboratory animals to assure the safety of vaccines according to several criteria, including clinical signs and change in body weight. Although the latter is used as an evaluation index in BSTs, there have been no reports on the internal changes that affect body weight during the test period. Therefore, we analyzed BST via pathological examination of the tested animals. Here, BSTs were performed for 176 batches using mice and 126 batches using of guinea pigs. Most of the gross findings could be classified into four lesion types (nodules, adhesions, ascites, no apparent signs), with only one vaccine inducing lesions that could not be classified into any of these four types. Histopathological examination revealed that the reactions caused by BST were pyogenic and/or granulomatous inflammation. Nodular or adhesive lesions comprised more severe pyogenic granulomatous inflammation than ascites or cases with no apparent gross lesions. These nodular or adhesive lesions were more frequently induced by vaccines that contained an adjuvant than by vaccines that did not contain an adjuvant. The cases with “exceptional” gross findings histologically presented severe necrosis of the hematopoietic system. Additional testing showed that these “exceptional” lesions were induced when a specific type of light liquid paraffin was injected along with other vaccine additives. Our results show that body weight loss and/or lesions during BST were induced by proinflammatory properties of the tested vaccines and that BST is a sensitive method for detecting unexpected effects of vaccine components. 相似文献
2.
Maryam Moradi Hadi Hajarian Hamed Karamishabankareh Leila Soltani Bijan Soleymani 《Reproduction in domestic animals》2021,56(2):263-269
Pre-conceptual sex selection is still a highly debatable process whereby X and Y chromosome bearing spermatozoa are isolated before oocyte fertilization. Recently, magnetic nanoparticles (MNP) have been used to determine X and Y chromosomes bearing spermatozoa as a result of searching for a cheap, highly efficient method using non-toxic materials. This study aimed to recover the sperm bearing X chromosomes in ram with different concentrations of MNP and then evaluate the success of this method using polymerase chain reaction (PCR). Ram sperms were divided into four groups, treated with 0 (control), 50, 100 and 200 μg/ml MNP, respectively. MNP was used to restore sperm cells bearing X chromosomes. Upon recovery, the PCR was performed to identify the X and Y sperms, Methyl ThiazoleTetrazolium (MTT), to assess MNP toxicity and sperm viability and acridine orange (AO) to evaluate sperm DNA integrity. The results of PCR revealed that the treatment of spermatozoa- bearing X chromosomes with 50 μg/ml MNP had the highest effects on the recovery of X sperm rather than the other concentrations of MNP. However, the concentrations of MNP did not have any toxic effects on spermatozoa, sperm viability and, DNA integrity, but the high concentration of MNP (200 μg/ml) significantly reduced DNA integrity. According to MTT and AO results, the concentrations of MNP used in this study had no toxic effects on spermatozoa and did not reduce the sperm viability and DNA integrity, except that 200 μg/ml MNP significantly reduced DNA integrity. 相似文献
3.
4.
M. Skrzypczyńska A. Sudo?-Kornalewicz A. Kornalewicz J. Biczak S. Rodkiewicz 《Anzeiger für Sch?dlingskunde》2001,78(4):138-143
A study was conducted on the insects infesting seeds and cones of Abies alba Mill. in the Gorce National Park, in southern Poland during 1996–1998. A total of 856 cones were collected from 66 trees growing at 11 sites located in partially or strictly protected forest reserves. Of this sample, 217 cones with 54,958 seeds were dissected. This study yielded 5,816 specimens of insects, namely: seminiphagous Resseliella piceae Seitn., Megastigmus suspectus Borr. as well as conophagous Earomyia impossibile Morge, Barbara herrichiana Obr. and Dioryctria abietella (Den. et Schiff.). The most abundant species was R. piceae with 4,186 individuals. The index of cone infestation was calculated. It showed great variability between forest reserves and study-years. The numbers of insect individuals, numbers of viable and infertile seeds, and seeds damaged by M. suspectus and R. piceae, in both strict and partial reserves in the consecutive years were compared statistically. The test of significant differences between two frequencies at 0.05 level was used in statistical analysis. In both strict and partial reserves, statistically significant differences were found between studied variables. 相似文献
5.
Wasfi IA Barezaiq IM Alkatheeri N Hadi AA Boni NS Almuhrami AM Elghazali M 《Research in veterinary science》1999,66(2):159-160
The pharmacokinetics of promethazine were determined in seven camels (Camelus dromedarius) after an intravenous dose of 0.5 mg kg body weight.-1 The data obtained (median and range) were as follows: the elimination half-life (t1/2 beta) was 5.62 (2.84-6.51) h; the steady state volume of distribution (Vdss) was 8.90 (7.10-12.00) L kg-1, total body clearance (CT) was 24.5 (17.22-33.65) ml kg-1 min-1 and renal clearance (Clr) was 4.81 (1.97-5.48) ml kg-1 min-1. 相似文献
6.
Elghazali M Wasfi IA Al Katheeri NA Hadi AA Hussein MM 《Journal of veterinary pharmacology and therapeutics》2002,25(1):43-48
The pharmacokinetics of etamiphylline were determined after an intramuscular (i.m.) dose of 3.5 mg/kg body weight in six healthy camels. Furthermore, the metabolites and drug detection time were evaluated. The data obtained median and (range) were as follows: the terminal elimination half-life (t(1/2 beta), h) was 3.04 (2.03-3.62); apparent total body clearance (Cl/F, L/h/kg) was 1.27 (0.74-2.99); the apparent volume of distribution at steady state (V(ss)/F, L/kg) was 4.94 (3.57-12.54); and renal clearance (Cl(r), L/h/kg) determined in two camels was 0.005 and 0.004, respectively. The detection time of etamiphylline in urine after an i.m. dose of 3.5 mg/kg body weight ranged between 12 and 13 days. Three etamiphylline metabolites were tentatively identified in camels urine: The first one desethyletamiphylline was the main metabolite and resulted from N-deethylation of etamiphylline had a molecular weight of 251, and was detected in urine for about 13-14 days. Theophylline (molecular weight 180) was the second metabolite and resulted from ring N-dealkylation of etamiphylline. It was present in small amounts and was detected for about 5 h after drug administration in urine. The third metabolite, possibly resulted from demethylation of etamiphylline, had a molecular weight of m/z 265, and was present in small amounts and was detected in urine for about 5 h after drug administration. 相似文献
7.
Yusuf Bozkurt Fatih Öğretmen Uğur Erçin Ümmügülsüm Yιldιz 《Aquaculture Research》2008,39(15):1666-1672
In this research, the mineral and organic composition of the seminal plasma, physical spermatological parameters and their physiological relationships were investigated in grass carp (Ctenopharyngodon idella). The seminal plasma contained 98.14±5.23 mM L?1 (Na+), 380.85±25.95 mM L?1 (K+), 30.25±4.96 mg dL?1 (Ca2+), 19.16±1.70 mEq L?1 (Mg2+), 1.36±0.11 mg dL?1 glucose, 0.37±0.08 g dL?1 total protein, 12.02±1.18 mg dL?1 cholesterol, 14.85±1.50 mg dL?1 triglyceride and 43.5±9.56 mg dL?1 urea. The following spermatological parameters were found: sperm volume 14.44±1.16 mL, sperm motility 80.60±1.55%, movement duration 67.68±4.32 s, density 15.43±0.72 × 109 mL?1, total density 337.43+45.86 × 109 and pH 7.24±0.17. The Na+ and Ca2+ ions correlated negatively with spermatozoa motility (r=?0.453, P>0.05 and r=?0.192, P>0.05) respectively. The K+ ion correlated positively with spermatozoa motility (r=0.545, P>0.05). But a statistically significant correlation was not observed between sperm motility and seminal plasma parameters. The following correlations were observed between mineral and organic components. The Mg2+ was positively correlated with glucose and cholesterol (r=0.692, P<0.05 and r=0.680, P<0.05) respectively. A highly significant positive relationship was also found between Mg2+ and total protein (r=0.837, P<0.01). On the other hand, a significantly negative relationship was found between Ca2+ and triglyceride (r=?0.639, P<0.05). These parameters should be considered when developing procedures for either artificial fertilization or for cryopreservation of grass carp sperm. 相似文献
8.
Five morphinoid alkaloids have been isolated from Dehaasia longipedicellata, namely (-) pallidine, a new alkaloid (+) pallidinine (1), (+)-milonine, (-) 8,14-dehydrosalutaridine and (-) sinoacutine. 相似文献
9.
Antti Otsamo Tjuk Sasmito Hadi Göran Ådjers Jussi Kuusipalo Risto Vuokko 《New Forests》1995,10(3):257-265
A species trial with 14 eucalypt species with randomized complete blocks design was planted on Imperata cylindrica dominated grassland in South Kalimantan, Indonesia in January 1987. The site was prepared mechanically. No fertilizers were used. At the age of three years E. camaldulensis had clearly the highest survival rate (95%) and mean annual increment (5.9 m3ha–1a–1) compared with the other species tried. It was followed by E. urophylla, E. deglupta, E. pellita, E. tereticornis, E. alba, E. torelliana, E. intermedia, E. grandis and E. pilularis. Four other species, E. phaeotricha, E. resinifera, E. cloeziana and E. microcorys died either in the nursery or immediately after planting. All the eucalypts tried had narrow crowns and open canopies. It is suggested that eucalypts are not recommendable for grassland reforestation under minimum plantation tending. Effects of intensive weeding, spacing, fertilizing and performance in second rotation or mixed stands should be tested to elaborate the suitability of eucalypts on grasslands. 相似文献
10.
BACKGROUND: Busulfan, a cytotoxic drug, which is currently used as a chemotherapeutic agent, has many side effects on different body organs. In this research, the effects of busulfan on sperm parameters and microstructure of mouse testis were investigated. METHODS: Busulfan was injected intrapretoneally at 10, 20, 30, 40 and 50 mg/kg and testes were removed after 4, 6 and 8 weeks, weighed and processed for light microscopic examination. Transverse and cross section diameters of testes, seminiferous tubules diameters, percentage of different types of tubules, epithelium thickness, spermatogenic cell numbers and capsule thickness as well as the sperm parameters in epididymis were measured. RESULTS: There was a significant decline in sperm numbers and marked changes in testes structures. Almost 8 weeks after the injection of drug, some of the changes are reversed. Accordingly, the changes in percent of normal tubules without sperm, abnormal tubules and capsule thickness were increased until 6 weeks of drug administration, the changes declined thereafter. CONCLUSION: In general, busulfan caused a decrease in all analyzed parameters (except capsule thickness, normal tubules without sperm and abnormal tubules), probably due to the arrest of spermatogenesis. Our results also revealed that some of the changes are reversible and dose dependent. 相似文献