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1.
Maryam Moradi Hadi Hajarian Hamed Karamishabankareh Leila Soltani Bijan Soleymani 《Reproduction in domestic animals》2021,56(2):263-269
Pre-conceptual sex selection is still a highly debatable process whereby X and Y chromosome bearing spermatozoa are isolated before oocyte fertilization. Recently, magnetic nanoparticles (MNP) have been used to determine X and Y chromosomes bearing spermatozoa as a result of searching for a cheap, highly efficient method using non-toxic materials. This study aimed to recover the sperm bearing X chromosomes in ram with different concentrations of MNP and then evaluate the success of this method using polymerase chain reaction (PCR). Ram sperms were divided into four groups, treated with 0 (control), 50, 100 and 200 μg/ml MNP, respectively. MNP was used to restore sperm cells bearing X chromosomes. Upon recovery, the PCR was performed to identify the X and Y sperms, Methyl ThiazoleTetrazolium (MTT), to assess MNP toxicity and sperm viability and acridine orange (AO) to evaluate sperm DNA integrity. The results of PCR revealed that the treatment of spermatozoa- bearing X chromosomes with 50 μg/ml MNP had the highest effects on the recovery of X sperm rather than the other concentrations of MNP. However, the concentrations of MNP did not have any toxic effects on spermatozoa, sperm viability and, DNA integrity, but the high concentration of MNP (200 μg/ml) significantly reduced DNA integrity. According to MTT and AO results, the concentrations of MNP used in this study had no toxic effects on spermatozoa and did not reduce the sperm viability and DNA integrity, except that 200 μg/ml MNP significantly reduced DNA integrity. 相似文献
2.
Bijan Soleymani Kamran Mansouri Mohsen Rastegari-Pouyani Shahram Parvaneh Fatemeh Khademi Mehdi Sharifi Tabar Ali Mostafaie 《Reproduction in domestic animals》2021,56(2):270-277
Separation of X and Y chromosome-bearing sperm is an appropriate method for the selection of desired sex of offspring to increase the profit in livestock industries. The purpose of this study was the production of a monoclonal antibody against recombinant bovine sex-determining region Y protein for separation Y sperm. The hybridoma cells from splenocytes of immunized female's balb/C mice and Sp2/0 cells were made. The binding affinity of our monoclonal antibody (mAbSRY2) was compared with mouse monoclonal SRY-15. The Western blot method indicated that mAbSRY2 successfully detected the rbSRY protein. The specificity and sensitivity of mAbSRY2 is comparable to SRY-15 commercially ones. The SRY gene in 100% of bull semen contains the Y chromosome that had the strongest binding affinity to mAbSRY2 was synthesized. In other words, the binding affinity of semen contains the X sperms near the negative control. In general, this immunological method can help to separate X from Y sperms. However, the mAbSRY2 is bind to Y-bearing sexed sperm, but in the future; the sexed sperms need to apply in farms. 相似文献
3.
Bijan Payandeh 《New Forests》1991,5(1):1-12
Data from 122 northeastern Ontario plots were used to develop composite site-productivity functions for black spruce (Picea mariana [Mill.] B.S.P.). A logistic model produced the best fit to the data. The resulting equations produce sets of polymorphic site-productivity curves with subsets of three curves, one for each of Ontario's Forest Ecosystem Classification Operational Groups (FEC OGs) 11, 12 and 14. For direct estimates of site productivity, site index was expressed as a function of stand height, age and FEC OG by employing the reciprocal of the logistic model. Application of the resulting equations and tables is demonstrated in an example. The procedures employed may be used to develop ecologically based site-productivity functions for other areas. 相似文献
4.
Bijan Payandeh 《New Forests》1988,2(3):145-160
Drainage and fertilization of an existing peatland black spruce (Picea mariana (Mill.) B.S.P.) stand and 24 possible methods of planting a clear-cut area near Cochrane, Ontario were evaluated by means of a recently developed forestry investment decision model, FIDME.Results indicate that, on the basis of the cost estimates used and assumptions made, drainage of the existing stand is the most economical treatment if it can reduce the rotation age by 30 years or more. Drainage combined with fertilization is the second best alternative if this treatment can reduce the rotation age by 40 years or more. Fertilization of the undrained site ranks a distant third, and then only if it reduces the rotation age by 10 years; otherwise, it will be uneconomical to apply.In the case of the clear-cut area, planting bare-root stock on a mechanically prepared but undrained site without weed control ranked first among the 24 alternatives considered; the same treatment, but with weed control included, ranked second. Planting containerized seedlings on a mechanically prepared but undrained site with weed control ranked third, followed by planting bare-root stock on a mechanically prepared and drained site with weed control. In contrast, planting containerized seedlings on an unprepared site and with no weed control ranked as the least cost-effective method considered. 相似文献
5.
The objective of this study was to quantify inherent spatial variability and spatial cross-correlation of the van Genuchten retention parameters and saturated hydraulic conductivity (Ks) of surface and subsurface layers in a calcareous Inceptisols (Khuzestan province, Iran) under sugarcane cropping. Measurements were performed on 100-cm3 undisturbed soil cores collected at 94 locations along a 30-m-long transect with horizontal sampling distance intervals of 0.3 and 1 m at soil depths of 0–40 and 40–80 cm, respectively. Spatial variability was investigated using conventional statistics and geostatistical techniques. Coefficient of variation (CV) varied from 8.2% (for shape parameter, n at 40–80 cm depth) to 256.7% (for Ks at 0–40 cm depth). The n parameter and saturated water content, θs, showed a small-scale spatial heterogeneity with a maximum CV of 11.3% for the first depth and 9.2% for the second depth. Most of the hydraulic parameters at both depths showed a spatial structure and convex experimental semivariograms with dominant spherical models with the influence range of 3.2–41 m. In most cases, the extent of spatial correlation scales of cross-semivariograms for pairs of cross-correlated hydraulic variables was found to be different with reference to those relating to the direct semivariograms of correlated variables. 相似文献
6.
Biswapriya Das Kalyan ChakrabartiSagarmoy Ghosh Bijan MajumdarSudipta Tripathi Ashis Chakraborty 《Industrial Crops and Products》2012,36(1):415-419
Four bacterial strains with high balanced polygalacturonase (PG) and pectin lyase (PNL), and xylanase with minimal cellulase activity were used in jute retting. The 16S rDNA analysis revealed that the organisms were: Bacillus sp. L6 (GQ891097), Bacillus pumilus strain EK-17 (GQ891098), B. pumilus strain Geo-03-422 (GQ891103) and B. pumilus strain IK-MB12-518F (GQ891105). The microorganisms in different combinations of consortia showed synergistic effect resulting in increased PG (35.52-46.61 IU/g cell wet weight), PNL (39.79-72.12 U/ml), xylanase (0.705-0.840 μmol/ml/min) and little cellulase (0-0.153 μmol/ml/min) activities. The organisms in different combinations of consortia reduced the retting period from 11 to 13 days as compared to 19 days in the control. Microbial inoculation produced remarkable improvement in jute fibre strength (26.62-28.91 g/tex) and fineness (2.76-2.92 tex) over control. The pH of the post-retting water samples became acidic, and the electrical conductivity (Ec), chemical oxygen demand (COD) and hardness increased. The organisms could be adopted in industrial application for extraction of jute fibre. 相似文献
7.
Modal-based damage-detection algorithms were used to identify the location of defects commonly found in timber and to estimate
their severities. In this study, the authors propose modifications to an existing damage-detection algorithm for locating
and evaluating damage by comparing the modal strain energy before and after damage using the first two flexural modes of vibration.
Experimental verification was performed on pin-pin supported timber beams by employing the algorithms with extracted modal
parameters using experimental modal analysis. Single and multiple cases of damage used to simulate pocket(s) of rot with various
severities were inflicted by removing sections of timber beam specimens. The proposed damage indicator, computed from the
first two flexural modes, was capable of detecting all damage locations. It was also able to estimate, with reasonable accuracy,
the severity of damage in term of loss of sectional moment of inertia. The modified damage index method is generally reliable
in detecting the location and estimating the severity of simulated defects in timber beams. 相似文献
8.
Davoud Akhzari Majid Khedmati Ali Reza Soleymani 《Communications in Soil Science and Plant Analysis》2016,47(20):2261-2270
A pot experiment based on completely randomized factorial arrangement design with four replications was conducted under greenhouse condition at Malayer University, Malayer, Iran. Pots contained a mixture of soil and different concentrations (0% and 60% w/w) of vermicompost. Plants were exposed to three lead nitrate concentrations: 0, 4, and 8 mM. Based on the results of this research, shoot height and root length of the studied plants significantly decreased with increased lead concentrations in all vermicompost application levels. Generally, plants exposed to lower levels of lead and vermicompost application had higher root:shoot ratio. The survival capacity of all the studied plants was significantly reduced with increased lead concentrations, but increased with increased vermicompost application level. The total protein content increased with decrease in lead nitrate concentration, but decreased with decrease in vermicompost application. Generally, translocation factor increased significantly as vermicompost application rate increased. The highest root concentration factor of lead was found in V1×Pb2 as compared to the other treatment levels. Generally, tolerance index values of all the studied plants were significantly higher in the lower lead concentration treatments. 相似文献
9.
Safflower (Carthamus tinctorius L.) is an important oil seed crop with growing importance in many countries around the world. However, safflower fly (Acanthiophilus helianthi Rossi) is one of the main limiting factors to expand the production area of the crop in several countries. Since host plant-resistance is the most efficient method for pest management, we evaluated a germplasm of wild accessions (Carthamus oxyacanthus Bieb.) with mostly brown–black seed coat color along with some white-seeded cultivated safflower genotypes for resistance to safflower fly. The results showed that there was a significant difference between two species for safflower fly damage with all cultivated genotypes being susceptible. However, for the accessions of C. oxyacanthus, a wide range of resistance was observed. In a choice experiment and natural infestation, 35.3–78.9 percent of heads per plant were infested in genotypes of C. tinctorius while for the accessions of C. oxyacanthus this was much smaller and ranged from 1.6 to 13.6%. In addition, percentage of seed yield loss per infested plant was more drastic in cultivated safflower (29.0–72.8%) than the wild accessions (0.0–21.4%). In the no-choice experiment, fly population was dramatically decreased in the wild accessions due to larval mortality and possibility of antibiosis. There was a strong relationship between brown–black seed coat color and resistance to safflower fly indicating the possibility of using this trait in breeding programs of safflower to develop fly-resistant cultivars. 相似文献
10.
Vikash Babu Syed Khalid Pasha Govind Gupta C. B. Majumdar Bijan Choudhury 《Fibers and Polymers》2014,15(1):24-29
Polyacrylonitrile (PAN) is a widely used polymer in the textile industry. PAN contains cyano groups on the surface due to which it possess low hydrophilicity and limits its application. Thus, there is a need to modify the functional groups on the surface of PAN for its industrial demand to improve moisture uptake, dyeability with ionic dyes, without affecting mechanical properties. A number of strategies such as chemical treatment, plasma treatment, enzymatic treatment etc. have been applied for the surface modification of polymer but enzymatic treatment are advantageous over plasma treatment and chemical treatment. In enzymatic treatment, reaction is limited to polymer surface only, and provides milder condition with less damage to polymer. In present study, it was found that enzyme system of Amycolatopsis sp.IITR215 was effective enzyme system for modification of surface nitrile groups of polyacrylonitrile. PAN powder was treated with the cell free extract of Amycolatopsis sp.IITR215 and it was found that the nitrile metabolizing enzymes of this strain were efficiently able to transform -CN to -COOH groups present on the surfaces of PAN powder. The formation of carboxyl group was quantified by ammonia released and dye binding assay. Further, confirmation of carboxyl group on polymer was done by FTIR and XPS. This study indicates that, specific adsorption of enzyme probably plays an important role in the enzymatic surface modification of polymer. 相似文献