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1.
ECOLOGY OF MYCOPLASMAS IN CLINICALLY HEALTHY CATS 总被引:1,自引:0,他引:1
Mycoplasmas were isolated from various sites and organs of a series of 319 clinically healthy cats. They include M. felis, M. gateae, M. Arginini, A. laidlawii, feline ureaplasmas, M. pulmonis, M. arthritidis, and M. gallisepticum. In addition, there were 10 strains of mycoplasmas which could not be identified with the specific antiserums by growth inhibition tests. Antibody against M. felis was demonstrated by haemagglutination-inhibition and complement-fixation tests in cats which were over 6 months of age. However, no such antibody against M. felis was detected in animals which were less than 6 months old. No antibody against A. laidlawii, M. gateae, M. arginini and feline ureaplasmas was demonstrated by the same serological methods. The significance of these mycoplasmas in cats is described. 相似文献
2.
Canapp SO Farese JP Schultz GS Gowda S Ishak AM Swaim SF Vangilder J Lee-Ambrose L Martin FG 《Veterinary surgery : VS》2003,32(6):515-523
OBJECTIVE: To evaluate the effects of topical glycyl-L-histidyl-L-lysine tripeptide-copper complex (TCC; Iamin 2% Gel; Procyte Corporation, Redmond, WA) on healing in ischemic open wounds. STUDY DESIGN: Experimental study. SAMPLE POPULATION: Twenty-four adult male Sprague-Dawley rats. METHODS: Rats were divided into 3 groups: topical TCC, topical TCC vehicle (hydroxypropyl-methylcellulose), and no treatment (control). Six-mm-diameter, full-thickness wounds were created within an ischemic bipedicle skin flap on the dorsum of each rat. Each day, for 13 days, wound margins were traced, and the TCC and TCC vehicle groups were treated topically. Tracings were scanned, and wound perimeter and area were calculated. On days 6, 10, and 13, selected wounds were biopsied and analyzed for tumor necrosis factor alpha (TNF-alpha) and matrix metalloproteinases (MMP) 2 and 9. RESULTS: A significant decrease in wound area was seen in the TCC group, but not the vehicle group, when compared with the control group on days 3 to 5, 6 to 9, and 11 to 13 and when TCC was compared with TCC vehicle on days 3 and 9. On day 13, initial wound area had decreased by 64.5% in the TCC group, 45.6% in the vehicle group, and 28.2% in the control group. On days 6, 10, and 13, TCC-treated wounds contained significantly lower concentrations of TNF-alpha and MMP-2 and MMP-9 than control wounds. CONCLUSION: Topical TCC resulted in accelerated wound healing in ischemic open wounds. CLINICAL RELEVANCE: Topical TCC is an effective stimulant of healing of ischemic open wounds in rats and may have an application for the treatment of chronic wounds in other species. Clinical evaluation of topical TCC is warranted. 相似文献
3.
Guangyu Wang Tongli Wang Haijun Kang Shari Mang Brianne Riehl Brad Seely Shirong Liu Futao Guo Qinglin Li John L. Innes 《林业研究》2016,27(3):469-488
Climate change is a threat to the stability and productivity of forest ecosystems throughout the Asia-Pacific region. The loss of forests due to climate-induced stress will have extensive adverse impacts on biodiversity and an array of ecosystem services that are essential for the maintenance of local economies and public health. Despite their importance, there is a lack of decision-support tools required to evaluate the potential effects of climate change on Asia-Pacific ecosystems and economies and to aid in the development of regionally appropriate adaptation and mitigation strategies. The project Adaptation of Asia-Pacific Forests to Climate Change, summarized herein, aims to address this lack of knowledge and tools and to provide support for regional managers to develop effective policy to increase the adaptive capacity of Asia-Pacific forest ecosystems. This objective has been achieved through the following activities: (1) development of a high-resolution climate downscaling model, ClimateAP, applicable to any location in the region; (2) development of climate niche models to evaluate how climate change might affect the distribution of suitable climatic conditions for regionally important tree species; (3) development and application of forest models to assess alternative management strategies in the context of management objectives and the projected impacts of climate change; (4) evaluation of models to assess forest fire risk and the relationship between forest fire and climate change; (5) development of a technique to assess ecosystem carbon storage using LiDAR; and (6) evaluation of how vegetation dynamics respond to climate change using remote sensing technology. All project outputs were developed with a focus on communication and extension to facilitate the dissemination of results to regional forest resource managers to support the development of effective mitigation and adaptation policy. 相似文献
4.
5.
A persistent oxygen anomaly reveals the fate of spilled methane in the deep Gulf of Mexico 总被引:3,自引:0,他引:3
Kessler JD Valentine DL Redmond MC Du M Chan EW Mendes SD Quiroz EW Villanueva CJ Shusta SS Werra LM Yvon-Lewis SA Weber TC 《Science (New York, N.Y.)》2011,331(6015):312-315
Methane was the most abundant hydrocarbon released during the 2010 Deepwater Horizon oil spill in the Gulf of Mexico. Beyond relevancy to this anthropogenic event, this methane release simulates a rapid and relatively short-term natural release from hydrates into deep water. Based on methane and oxygen distributions measured at 207 stations throughout the affected region, we find that within ~120 days from the onset of release ~3.0 × 10(10) to 3.9 × 10(10) moles of oxygen were respired, primarily by methanotrophs, and left behind a residual microbial community containing methanotrophic bacteria. We suggest that a vigorous deepwater bacterial bloom respired nearly all the released methane within this time, and that by analogy, large-scale releases of methane from hydrate in the deep ocean are likely to be met by a similarly rapid methanotrophic response. 相似文献
6.
Premier RR Jacobs HJ Lofthouse SA Sedgmen BJ Meeusen EN 《Veterinary immunology and immunopathology》2004,98(1-2):77-84
The isotype-specific antibody responses of sheep immunised with keyhole limpet hemocyanin by a peripheral route (intramuscular (i.m.) injection) were compared to those induced by immunisation via different mucosal routes: (1) intra-nasal spray; (2) rectal deposition with cholera toxin; (3) injection into the mucosa of the small intestine or rectum. Antigen-specific IgG1 antibodies were induced in the i.m., intra-intestinal and intra-rectal injection groups and in a proportion of the cholera toxin immunised sheep, but not in the intra-nasal immunisation group. IgA was the only antibody isotype detected in serum collected from the intra-nasal immunisation group. No significant differences in serum IgA levels were detected in any of the mucosal immunisation groups as compared to the i.m. injection group. In contrast, analysis of the in vitro antibody profiles secreted by circulating antibody-secreting cells (ASC) revealed significantly higher IgA responses in the supernatants from all mucosal immunisation groups. This suggests that the measurement of antibodies secreted by circulating ASCs may be a better correlate of local mucosal responses in ruminants, as has been previously demonstrated in human studies. In addition to IgG1 and IgA responses, immunisation by direct injection of antigen formulations into the intestinal and rectal mucosa were the only groups to induce consistently high IgG2 antibodies in serum and ASC cultures. 相似文献
7.
Shari K. Hanson Jeffrey E. Hill Craig A. Watson Roy P. E. Yanong Richard Endris 《Journal of aquatic animal health》2013,25(1):30-34
Abstract The effect of 0.2% emamectin benzoate (SLICE; Intervet/ Schering-Plough Animal Health, Roseland, New Jersey) administered in top-dressed, pelleted commercial fish feed was evaluated for control of freshwater Argulus sp. in goldfish Carassius auratus and koi carp, a variant of common carp Cyprinus carpio, in freshwater aquaria at 24–25°C. Sixteen individually housed goldfish were each exposed to 37 Argulus. The number of fish lice attached to each fish at the start of the experiment was not determined; however, the total number of motile fish lice in each aquarium (on fish and in the water) was determined at the start and end of each experiment. Eight goldfish were fed the control diet (0 μg · kg fish biomass?1 · d?1) and eight were fed the medicated diet (50 μg · kg fish biomass?1 · d?1) for seven consecutive days. After treatment, fish louse infestation in controls was 20.5 ± 1.5 (mean ± SE) lice per fish. No Argulus were found on fish in the treated group. In a separate experiment, 10 individually housed koi were each exposed to 128 Argulus. Five koi were fed the control diet and five were fed a low-dose medicated diet (5 μg · kg fish biomass?1 · d?1) for 7 d. After treatment, fish louse infestation among the controls was 14.6 ± 3.8 lice per koi. No Argulus were found on koi in the treated group. Hence, a 7-d regimen of oral emamectin benzoate controlled experimental infestation of Argulus when administered to goldfish at 50 μg · kg fish biomass?1 · d?1 and to koi at 5 μg · kg fish biomass?1 · d?1. Received March 24, 2010; accepted January 17, 2011 相似文献
8.
Sophy A. Jesty Seung Woo Jung Jonathan M. Cordeiro Teresa M. Gunn José M. Di Diego Shari Hemsley Bruce G. Kornreich Giles Hooker Charles Antzelevitch N. Sydney Moïse 《Journal of Veterinary Cardiology》2013,15(1):5-14
ObjectiveTo further characterize arrhythmic mechanisms in German shepherd dogs (GSDs) affected with inherited ventricular arrhythmias by evaluating intracellular calcium cycling and expression of calcium handling genes.AnimalsTwenty five GSDs, 9 backcross dogs, and 6 normal mongrel dogs (controls) were studied. The GSDs and backcross dogs were from a research colony of inherited ventricular arrhythmias. The control research dogs were purchased.MethodsAction potentials (APs) and pseudo-electrocardiograms (ECG) were recorded from left ventricular (LV) wedge preparations of GSDs and normal dogs. Midmyocardial (Mid) LV cells from GSDs and normal mongrels were isolated by enzymatic digestion. Cells were either field stimulated or voltage clamped and calcium transients were measured by confocal microscopy using the indicator Fluo-3AM. Expression of calcium handling genes was measured by quantitative RT-PCR.ResultsMean calcium transient decay (tau) was not different between affected GSDs and control dogs, but striking cell-to-cell variability for tau was observed within affected GSDs and between affected GSDs and controls (P < 0.0001 each); within-dog variability accounted for 75% of total variability. Calcium sparks and afterdepolarizations occurred in GSD but not control cells. ATP2A2/SERCA2a expression was significantly reduced (P = 0.0063) in affected GSDs and inversely correlated (P = 0.0006) with severity of ventricular arrhythmias.ConclusionsGerman shepherd dogs with inherited ventricular arrhythmias have electrophysiologic abnormalities in calcium cycling associated with reduced ATP2A2/SERCA2a expression. These animals provide a unique opportunity to study calcium remodeling at the genetic and molecular level in familial ventricular arrhythmias. 相似文献
9.
Guangyu Wang Shari L.Mang Brianne Riehl Jieying Huang Guibin Wang Lianzhen Xu Kebiao Huang John Innes 《林业研究》2019,(1):277-293
Expert opinions have been used in a variety of fields to identify relevant issues and courses of action. This study surveys experts in forestry and climate change from the Asia–Pacific region to gauge their perspectives on the impacts of climate change and on the challenges faced by forest adaptation in the region, and explores recommendations and initiatives for adapting forests to climate change. There was consensus regarding the impacts of climate change on forest ecosystems and on economic sectors such as agriculture and forestry. Respondents also indicated a lack of public awareness and policy and legislation as challenges to addressing climate change. However, the results indicate differences in opinion between regions on the negative impacts of climate change and in satisfaction with actions taken to address climate change,highlighting the need for locally specific policies and research. The study presents specific recommendations to address issues of most concern, based on subregion and professional affiliation throughout the Asia–Pacific region.The results can be used to improve policy and forest management throughout the region. This research will also provide valuable suggestions on how to apply research findings and management recommendations outside of the AP region. The conclusions should be communicated relative to the level of the research and the target audience,ensuring that scientific findings and management recommendations are effectively communicated to ensure successful implementation of forest adaptation strategies. 相似文献
10.
Doni Febri Suhaimi Nurul Shamsinah Mohd Mohamed Zulqarnain Ishak Nazri Mispan Muhamad Shakirin 《植物病害和植物保护杂志》2019,126(6):491-494
Journal of Plant Diseases and Protection - Leaf blight is one of the most serious threats to rice agroecosystems worldwide, and the yield reduction due to this disease can be as high as 70%. For... 相似文献