A new yellow mosaic disease of chayote in India     

B. Mandal  S. Mandal  S. S. Sohrab  K. B. Pun  A. Varma 《Plant pathology》2004,53(6):797-797

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Copper nanoparticle induced macromutation in Macrotyloma uniflorum (Lam.) Verdc. (Leguminosae): a pioneer report     

Sandip Halder  Aninda Mandal  Debadrito Das  Sudha Gupta  Asoke Prasun Chattopadhyay  Animesh Kumar Datta 《Genetic Resources and Crop Evolution》2015,62(2):165-175

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Recovery of sperms bearing X chromosomes with different concentrations of magnetic nanoparticles in ram     

Maryam Moradi  Hadi Hajarian  Hamed Karamishabankareh  Leila Soltani  Bijan Soleymani 《Reproduction in domestic animals》2021,56(2):263-269

Pre-conceptual sex selection is still a highly debatable process whereby X and Y chromosome bearing spermatozoa are isolated before oocyte fertilization. Recently, magnetic nanoparticles (MNP) have been used to determine X and Y chromosomes bearing spermatozoa as a result of searching for a cheap, highly efficient method using non-toxic materials. This study aimed to recover the sperm bearing X chromosomes in ram with different concentrations of MNP and then evaluate the success of this method using polymerase chain reaction (PCR). Ram sperms were divided into four groups, treated with 0 (control), 50, 100 and 200 μg/ml MNP, respectively. MNP was used to restore sperm cells bearing X chromosomes. Upon recovery, the PCR was performed to identify the X and Y sperms, Methyl ThiazoleTetrazolium (MTT), to assess MNP toxicity and sperm viability and acridine orange (AO) to evaluate sperm DNA integrity. The results of PCR revealed that the treatment of spermatozoa- bearing X chromosomes with 50 μg/ml MNP had the highest effects on the recovery of X sperm rather than the other concentrations of MNP. However, the concentrations of MNP did not have any toxic effects on spermatozoa, sperm viability and, DNA integrity, but the high concentration of MNP (200 μg/ml) significantly reduced DNA integrity. According to MTT and AO results, the concentrations of MNP used in this study had no toxic effects on spermatozoa and did not reduce the sperm viability and DNA integrity, except that 200 μg/ml MNP significantly reduced DNA integrity.  相似文献   

Production of monoclonal antibody against recombinant bovine sex-determining region Y (SRY) and their preferential binding to Y chromosome-bearing sperm     

Bijan Soleymani  Kamran Mansouri  Mohsen Rastegari-Pouyani  Shahram Parvaneh  Fatemeh Khademi  Mehdi Sharifi Tabar  Ali Mostafaie 《Reproduction in domestic animals》2021,56(2):270-277

Separation of X and Y chromosome-bearing sperm is an appropriate method for the selection of desired sex of offspring to increase the profit in livestock industries. The purpose of this study was the production of a monoclonal antibody against recombinant bovine sex-determining region Y protein for separation Y sperm. The hybridoma cells from splenocytes of immunized female's balb/C mice and Sp2/0 cells were made. The binding affinity of our monoclonal antibody (mAbSRY2) was compared with mouse monoclonal SRY-15. The Western blot method indicated that mAbSRY2 successfully detected the rbSRY protein. The specificity and sensitivity of mAbSRY2 is comparable to SRY-15 commercially ones. The SRY gene in 100% of bull semen contains the Y chromosome that had the strongest binding affinity to mAbSRY2 was synthesized. In other words, the binding affinity of semen contains the X sperms near the negative control. In general, this immunological method can help to separate X from Y sperms. However, the mAbSRY2 is bind to Y-bearing sexed sperm, but in the future; the sexed sperms need to apply in farms.  相似文献   

Photodegradation of hexythiazox in different solvent systems under the influence of ultraviolet light and sunlight in the presence of TiO2, H2O2, and KNO3 and identification of the photometabolites     

Mandal S  Joardar S  Das S  Bhattacharyya A 《Journal of agricultural and food chemistry》2011,59(21):11727-11734

The photodegradation of the carboxamide acaricide hexythiazox in three different solvent systems (aqueous methanolic, aqueous isopropanolic, and aqueous acetonitrilic solutions) in the presence of H(2)O(2), KNO(3), and TiO(2) under ultraviolet (UV) light (λ(max) ≥ 250 nm) and sunlight (λ(max) ≥290 nm) has been assessed in this work. The kinetics of photodecomposition of hexythiazox and the identification of photoproducts were carried out using liquid chromatography-mass spectrometry. The rate of photodecomposition of hexythiazox in different solvents followed first-order kinetics in both UV radiation and natural sunlight, and the degradation rates were faster under UV light than under sunlight. Hexythiazox was found to be more efficiently photodegraded in the presence of TiO(2) than in the presence of H(2)O(2) and KNO(3). Two major photoproducts were separated in pure form using column chromatography and identified according to IR, (1)H NMR, and mass spectral information as cyclohexylamine and 5-(4-chlorophenyl)-4-methylthiazolidin-2-one. Another nine photoproducts were identified according to LC-MS/MS spectral information. The plausible photodegradation pathways of hexythiazox were proposed according to the structures of the photoproducts.  相似文献   

Spatial variability of water retention parameters and saturated hydraulic conductivity in a calcareous Inceptisols (Khuzestan province of Iran) under sugarcane cropping     

Farzad Moradi  Bijan Khalili Moghaddam 《Archives of Agronomy and Soil Science》2016,62(12):1686-1699

The objective of this study was to quantify inherent spatial variability and spatial cross-correlation of the van Genuchten retention parameters and saturated hydraulic conductivity (K_s) of surface and subsurface layers in a calcareous Inceptisols (Khuzestan province, Iran) under sugarcane cropping. Measurements were performed on 100-cm³ undisturbed soil cores collected at 94 locations along a 30-m-long transect with horizontal sampling distance intervals of 0.3 and 1 m at soil depths of 0–40 and 40–80 cm, respectively. Spatial variability was investigated using conventional statistics and geostatistical techniques. Coefficient of variation (CV) varied from 8.2% (for shape parameter, n at 40–80 cm depth) to 256.7% (for K_s at 0–40 cm depth). The n parameter and saturated water content, θ_s, showed a small-scale spatial heterogeneity with a maximum CV of 11.3% for the first depth and 9.2% for the second depth. Most of the hydraulic parameters at both depths showed a spatial structure and convex experimental semivariograms with dominant spherical models with the influence range of 3.2–41 m. In most cases, the extent of spatial correlation scales of cross-semivariograms for pairs of cross-correlated hydraulic variables was found to be different with reference to those relating to the direct semivariograms of correlated variables.  相似文献   

Artificial polyploidy in medicinal plants: Advancement in the last two decades and impending prospects     

Umme Salma  Suprabuddha Kundu  Nirmal Mandal 《Journal of Crop Science and Biotechnology》2017,20(1):9-19

Medicinal plants are in huge demand since the consumption is widespread and ever-increasing globally. The conventional breeding programs are generally environmental dependent; prone to different biotic and abiotic stresses as well as the secondary metabolite content is too low to harvest. In this context, developing polyploid individuals artificially would be a remarkable approach to increase vigor and attain this objective. Polyploids often exhibit some morphological features that are different or greater in forms than their diploid progenies. Polyploidization can be induced by quite a few antimitotic agents. The most frequently used antimitotic chemicals are colchicine, trifluralin, and oryzalin. The whole method of induced chromosome doubling consists of a series of steps, including an induction phase, regrowth phase, and a confirmation technique to evaluate the rate of achievement. The induction phase depends on different factors, such as explant types, antimitotic agents, its different concentrations, and exposure durations. To evaluate the accomplishment of polyploidization, morphological or anatomical observations are recorded as a rapid method. However, chromosome count and flow cytometry are the most eminent method for absolute confirmation. Despite significant prospects of polyploidization, there has been very little research on medicinal plants. The current review gives an overview of the different parameters of in vitro chromosome doubling, the history of the technique, and progress made over the last two decades.  相似文献   

A comprehensive study on occurrence records of African neglected and underutilized weed species,Cleome gynandra L. (cat's whiskers) validating the ecogeographical range expansion in West Bengal,India     

Ekta Bhattacharya  Rahul Bose  Suparna Mandal Biswas 《Weed Biology and Management》2019,19(4):129-134

Cleome gynandra L., commonly known as cat's whiskers, is an erect, branched, annual herb, belonging to the family Cleomaceae. The species is thought to have originated in tropical Africa and Southeast Asia, and thereafter have spread to other tropical and subtropical countries in the Northern and Southern hemispheres. Cat's whiskers is a highly nutritious vegetable and also possesses numerous medicinal values, yet it is considered as a neglected and underutilized weed species (NUS) in most of the world. In India, C. gynandra is recorded in Assam, Gujarat, Kerala, Maharashtra, Rajasthan, Tamil Nadu, and Uttar Pradesh. There is no earlier report of this species in West Bengal, India. This report is the first record of natural occurrence of C. gynandra in West Bengal, India. The main objective of this report is to provide valid information about the invasion and naturalization of this species in a new geographical area supported with suitable data. Global distribution data of cat's whiskers was retrieved from available web resources and mapped using Quantum GIS software in order to validate the de novo nature of occurrence of this species in this region.  相似文献   

Contributing traits for nitrogen use efficiency in selected wheat genotypes and corollary between screening methodologies     

Rumesh Ranjan  Ashish Kumar  Swarupa Nanda Mandal 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(7):588-595

ABSTRACT

Nitrogen uptake being part of nitrogen use efficiency (NUE) is largely decided by root traits. Root traits variability has hardly been explored by breeders mainly because of difficulties in scoring. The hydroponic system requiring lesser space for precise phenotyping of large numbers of genotypes independently of the growing season can be a suitable alternative. However, the effectiveness of hydroponic screening methods needs to be verified under the soil condition of the field or pot. In the present study, root traits and NUE were investigated in 19 genotypes under two conditions (hydroponic and pipe filled with soil). Both environments revealed large variability for root traits and NUE under high and low N conditions establishing the absence of any direct selection for these traits in the past. Under both sets of experimentation, NUpE was largely responsible for improved nitrogen efficiency mainly because of higher root biomass. The significant association between the two screening methods i.e. hydroponic and pot filled with soil under both low and high N condition support large scale screening for root traits under hydroponic condition.  相似文献   

Fatal hydatid disease with involvement of the cerebrum in buffaloes (bos bubalis)     

P C Mandal 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1977,24(8):678-679

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