Recovery of sperms bearing X chromosomes with different concentrations of magnetic nanoparticles in ram     

Maryam Moradi  Hadi Hajarian  Hamed Karamishabankareh  Leila Soltani  Bijan Soleymani 《Reproduction in domestic animals》2021,56(2):263-269

Pre-conceptual sex selection is still a highly debatable process whereby X and Y chromosome bearing spermatozoa are isolated before oocyte fertilization. Recently, magnetic nanoparticles (MNP) have been used to determine X and Y chromosomes bearing spermatozoa as a result of searching for a cheap, highly efficient method using non-toxic materials. This study aimed to recover the sperm bearing X chromosomes in ram with different concentrations of MNP and then evaluate the success of this method using polymerase chain reaction (PCR). Ram sperms were divided into four groups, treated with 0 (control), 50, 100 and 200 μg/ml MNP, respectively. MNP was used to restore sperm cells bearing X chromosomes. Upon recovery, the PCR was performed to identify the X and Y sperms, Methyl ThiazoleTetrazolium (MTT), to assess MNP toxicity and sperm viability and acridine orange (AO) to evaluate sperm DNA integrity. The results of PCR revealed that the treatment of spermatozoa- bearing X chromosomes with 50 μg/ml MNP had the highest effects on the recovery of X sperm rather than the other concentrations of MNP. However, the concentrations of MNP did not have any toxic effects on spermatozoa, sperm viability and, DNA integrity, but the high concentration of MNP (200 μg/ml) significantly reduced DNA integrity. According to MTT and AO results, the concentrations of MNP used in this study had no toxic effects on spermatozoa and did not reduce the sperm viability and DNA integrity, except that 200 μg/ml MNP significantly reduced DNA integrity.  相似文献   

Production of monoclonal antibody against recombinant bovine sex-determining region Y (SRY) and their preferential binding to Y chromosome-bearing sperm     

Bijan Soleymani  Kamran Mansouri  Mohsen Rastegari-Pouyani  Shahram Parvaneh  Fatemeh Khademi  Mehdi Sharifi Tabar  Ali Mostafaie 《Reproduction in domestic animals》2021,56(2):270-277

Separation of X and Y chromosome-bearing sperm is an appropriate method for the selection of desired sex of offspring to increase the profit in livestock industries. The purpose of this study was the production of a monoclonal antibody against recombinant bovine sex-determining region Y protein for separation Y sperm. The hybridoma cells from splenocytes of immunized female's balb/C mice and Sp2/0 cells were made. The binding affinity of our monoclonal antibody (mAbSRY2) was compared with mouse monoclonal SRY-15. The Western blot method indicated that mAbSRY2 successfully detected the rbSRY protein. The specificity and sensitivity of mAbSRY2 is comparable to SRY-15 commercially ones. The SRY gene in 100% of bull semen contains the Y chromosome that had the strongest binding affinity to mAbSRY2 was synthesized. In other words, the binding affinity of semen contains the X sperms near the negative control. In general, this immunological method can help to separate X from Y sperms. However, the mAbSRY2 is bind to Y-bearing sexed sperm, but in the future; the sexed sperms need to apply in farms.  相似文献   

Composite site-productivity functions for Northeastern Ontario black spruce     

Bijan Payandeh 《New Forests》1991,5(1):1-12

Data from 122 northeastern Ontario plots were used to develop composite site-productivity functions for black spruce (Picea mariana [Mill.] B.S.P.). A logistic model produced the best fit to the data. The resulting equations produce sets of polymorphic site-productivity curves with subsets of three curves, one for each of Ontario's Forest Ecosystem Classification Operational Groups (FEC OGs) 11, 12 and 14. For direct estimates of site productivity, site index was expressed as a function of stand height, age and FEC OG by employing the reciprocal of the logistic model. Application of the resulting equations and tables is demonstrated in an example. The procedures employed may be used to develop ecologically based site-productivity functions for other areas.  相似文献   

Economic evaluation of forest drainage and fertilization in northern Ontario peatlands with an investment decision model     

Bijan Payandeh 《New Forests》1988,2(3):145-160

Drainage and fertilization of an existing peatland black spruce (Picea mariana (Mill.) B.S.P.) stand and 24 possible methods of planting a clear-cut area near Cochrane, Ontario were evaluated by means of a recently developed forestry investment decision model, FIDME.Results indicate that, on the basis of the cost estimates used and assumptions made, drainage of the existing stand is the most economical treatment if it can reduce the rotation age by 30 years or more. Drainage combined with fertilization is the second best alternative if this treatment can reduce the rotation age by 40 years or more. Fertilization of the undrained site ranks a distant third, and then only if it reduces the rotation age by 10 years; otherwise, it will be uneconomical to apply.In the case of the clear-cut area, planting bare-root stock on a mechanically prepared but undrained site without weed control ranked first among the 24 alternatives considered; the same treatment, but with weed control included, ranked second. Planting containerized seedlings on a mechanically prepared but undrained site with weed control ranked third, followed by planting bare-root stock on a mechanically prepared and drained site with weed control. In contrast, planting containerized seedlings on an unprepared site and with no weed control ranked as the least cost-effective method considered.  相似文献   

Physico-chemical Characteristics as Indicators of Starch Availability from Milled Rice     

I. Tetens  S.K. Biswas  L.V. Glitsø  K.A. Kabir  S.H. Thilsted  N.H. Choudhury 《Journal of Cereal Science》1997,26(3):355-361

The hypothesis was tested that certain physico-chemical characteristics might be used as indicators of total starch availability and rate of starch availability of milled rice. Milled unparboiled (uPB) and parboiled (PB) rice samples (n=93) were characterized using standardized methods of physical tests and chemical analyses and anin vitromethod was used for measuring the rate of starch digestion on a subsample of rice (n=26). The rice varieties were dominated by medium long, bold rice grain with high amylose rice and intermediate gelatinization temperature (GT), but a wide range in all characteristics was measured. Small amounts of resistant starch (RS) were measured in the cooked rice, indicating virtually complete starch availability. The RS of PB rice (0·4 g/100 g rice as eaten) was significantly (P<0·004) higher than the RS of uPB rice (0·1 g/100 g) however. The rate of starch digestion was significantly affected by both variety and parboiling. The starch digestion index (SDI) values of the PB samples (mean value 73·7) were significantly (P<0·001) lower than those of the uPB samples (mean value 79·0). The apparent amylose content (AC) was the strongest determinant for SDI in both uPB and PB rice. The widths and shapes of the raw grains and the elongation after cooking were correlated significantly with SDI values for the uPB rice, while the relatively mild parboiling procedure followed in this study eliminated this correlation. The minimum cooking times were correlated significantly with the SDI values in the uPB samples.  相似文献   

Metabolism of metamitron in goat following a single oral administration of a nontoxic dose level: a continued study     

Chanda D  Debnath SC  Das SK  Mandal TK  Bhattacharyya A  Choudhury A  Chakraborty AK 《Journal of agricultural and food chemistry》2004,52(24):7377-7381

Disposition kinetic behavior and metabolism studies of metamitron and its metabolite in terms of the parent compound were carried out in black Bengal goats after a single oral administration of a nontoxic oral dose at 30 mg kg(-1) of body weight. Metamitron was detected in the blood sample at 5 min (2.23 +/- 0.04 microg mL(-1)), maximum at 1 h (3.43 +/- 0.02 microg mL(-1)) and minimum at 12 h (0.41 +/- 0.01 microg mL(-1)), after a single oral administration. Metabolite [3-methyl-6-phenyl-1,2,4-triazin-5(4H)-one] in terms of the parent compound was detected in the blood sample at 5 min (0.47 +/- 0.006 microg mL(-1)), maximum at 6 h (5.12 +/- 0.02 microg mL(-1)) and minimum at 96 h (1.06 +/- 0.016 microg mL(-1)), after a single oral administration. The t(1/2 K) and Cl(B) values of metamitron were 3.63 +/- 0.05 h and 1.36 +/- 0.016 L kg(-1) h(-1), respectively, whereas the t(1/2K)(m) and Cl(B)(m) values of the metabolite were 38.15 +/- 0.37 h and 0.091 +/- 0.001 L kg(-1) h(-1), respectively, which suggested long persistence of the metabolite in blood and tissues of goat. Metamitron was excreted through feces and urine for up to 48 and 72 h, whereas the metabolite was excreted for up to 168 and 144 h, respectively. Metabolite alone contributed to 96 and 67% of combined recovery percentage of metamitron and metabolite against the administered dose in feces and urine of goat, respectively. All of the goat tissues except lung, adrenal gland, ovary, testis, and mammary gland retained the metabolite residue for up to 6 days after administration.  相似文献   

A note on canopy and air temperature equality     

Bhaskar Choudhury 《Agricultural and Forest Meteorology》1985,34(4):333-336

Well-watered crops having nearly identical ratio of intercept and slope of the “non-water-stressed baseline” would attain their equivalence point temperature at roughly identical air vapor pressure deficit. However, such a behavior of these crops might not prevail as the wet soil dries out progressively. The methods for calculating the equivalence point temperature due to Linacre (1964) and Paw U (1984) gave results which sometimes differed by more than 10 K.  相似文献   

Differential response to disturbance factors for the population of sympatric crocodilians (Gavialis gangeticus and Crocodylus palustris) in Katarniaghat Wildlife Sanctuary,India          下载免费PDF全文

Shikha Choudhary  B.C. Choudhury  G.V. Gopi 《水产资源保护:海洋与淡水生态系统》2017,27(5):946-952

Changes in ovary and testis and breeding fitness of the climbing perch,Anabas testudineus (Bloch, 1792), exposed to sub‐lethal concentrations of monocrotophos     

Sujata Mohapatra  Rajesh Kumar  Srianga Tilak Patnaik  Choudhury Suryakant Mishra  Lakshman Sahoo  Jitendra Kumar Sundaray 《Aquaculture Research》2020,51(8):3230-3236

Monocrotophos (MCP) is one of the organophosphate pesticides extensively used in agriculture and animal husbandry. The present study reports the effects of sub‐lethal concentrations of MCP on gonadosomatic index (GSI), gonadal histology and breeding fitness of the fish Anabas testudineus. The fish were exposed to three sub‐lethal concentrations of monocrotophos (T1: 3.5 mg/L, T2: 5.3 mg/L and T3: 10.6 mg/L) for 45 days with 12 fish (males and females in equal numbers) in each tank, and one group was kept as control (C) without any treatment. GSI decreased in all treatment groups in comparison with control. Significant decrease in fertilization and hatching rate was observed at all concentrations in comparison with control on 15, 30 and 45 days of monocrotophos exposure. The decreased vitellogenesis, disruption of follicular wall and oocyte atresia were observed in all the treated females at 30 and 45 days of exposure. Rupture of seminiferous tubules was noticed in all the treated males irrespective of exposure periods. The results thus indicated that monocrotophos could deleteriously impact the gonadal structure and function in A. testudineus, which could seriously impact the reproductive success of the animal.  相似文献   

Spatial variability of water retention parameters and saturated hydraulic conductivity in a calcareous Inceptisols (Khuzestan province of Iran) under sugarcane cropping     

Farzad Moradi  Bijan Khalili Moghaddam 《Archives of Agronomy and Soil Science》2016,62(12):1686-1699

The objective of this study was to quantify inherent spatial variability and spatial cross-correlation of the van Genuchten retention parameters and saturated hydraulic conductivity (K_s) of surface and subsurface layers in a calcareous Inceptisols (Khuzestan province, Iran) under sugarcane cropping. Measurements were performed on 100-cm³ undisturbed soil cores collected at 94 locations along a 30-m-long transect with horizontal sampling distance intervals of 0.3 and 1 m at soil depths of 0–40 and 40–80 cm, respectively. Spatial variability was investigated using conventional statistics and geostatistical techniques. Coefficient of variation (CV) varied from 8.2% (for shape parameter, n at 40–80 cm depth) to 256.7% (for K_s at 0–40 cm depth). The n parameter and saturated water content, θ_s, showed a small-scale spatial heterogeneity with a maximum CV of 11.3% for the first depth and 9.2% for the second depth. Most of the hydraulic parameters at both depths showed a spatial structure and convex experimental semivariograms with dominant spherical models with the influence range of 3.2–41 m. In most cases, the extent of spatial correlation scales of cross-semivariograms for pairs of cross-correlated hydraulic variables was found to be different with reference to those relating to the direct semivariograms of correlated variables.  相似文献