Contributions to the leafminer fauna (Diptera: Agromyzidae) from Turkey, with four new records     

Emine Çıkman  Hasan Sungur Civelek 《Phytoparasitica》2005,33(4):391-396

In a study carried out during 2002 and 2004 in Diyarbakıir and Mardin provinces, southeast Turkey, specimens were collected twice a month from cultivated and non-cultivated plants. Sixteen leafminer (Diptera: Agromyzidae) species were identified. Among them,Agromyza abiens Zetterstedt, 1848;Napomyza elegans (Meigen, 1830);Phytoliriomyza dorsata (Siebke, 1864); andPhytomyza aquilonia Frey, 1964 are new records for the Turkish leafminer http://www.phytoparasitica.org posting July 24, 2005.  相似文献   

Effect of transferring lignocellulose-degrading bacteria from termite to rumen fluid of sheep on in vitro gas production,fermentation parameters,microbial populations and enzyme activity          下载免费PDF全文

Ayoub AZIZI  Afrooz SHARIFI  Hasan FAZAELI  Arash AZARFAR  Arjan JONKER  Ali KIANI 《农业科学学报》2020,19(5):1323-1331

The digestive tract of termite(Microcerotermes diversus) contains a variety of lignocellulose-degrading bacteria with exocellulases enzyme activity, not found in the rumen, which could potentially improve fiber degradation in the rumen. The objectives of the current study were to determine the effect of inoculation of rumen fluid(RF) with three species of bacteria isolated from termite digestive tract, Bacillus licheniformis, Ochrobactrum intermedium, and Microbacterium paludicola, on in vitro gas production(IVGP), fermentation parameters, nutrient disappearance, microbial populations, and hydrolytic enzyme activities with fibrous wheat straw(WS) and date leaf(DL) as incubation substrate. Inoculation of RF with either of three termite bacteria increased(P0.05) ammonia-N concentration compared with the control group(free of termite gut bacteria). Termite bacteria inoculation had no effect(P0.05) on gas production characteristics, dry matter, organic matter and neutral detergent fiber disappearance, pH, and concentration and composition of volatile fatty acids. Population of proteolytic bacteria and protozoa, but not cellulolytic bacteria, were increased(P0.05) when RF was inoculated with termite bacteria with both WS and DL substrates. Inoculation of RF with termite bacteria increased protease activity, while activities of carboxymethyl-cellulase, microcrystalline-cellulase, α-amylase and filter paper degrading activity remained unchanged(P0.05). Overall, the results of this study indicated that transferring lignocellulose-degrading bacteria, isolated from digestive tract of termite, to rumen liquid increased protozoa and proteolytic bacteria population and consequently increased protease activity and ammonia-N concentration in vitro, however, no effect on fermentation and fiber degradation parameters were detected. These results suggest that the termite bacteria might be rapidly lysed by the rumen microbes before beneficial effects on the rumen fermentation process could occur.  相似文献   

Effects of AG1 and AG2 QTLs on Nonstructural Carbohydrate and Seed Management Options for Rice Seedling Growth and Establishment under Flooding Stress     

Mondal Satyen  Jamil Hasan M.  Ahmed Tofayel  Giashuddin Miah M.  C. Sta Cruz Pompe  M. Ismail Abdel 《水稻科学》2020,27(6):515-528

Rice (Oryza sativa) plants acquired excess photosynthates in the form of nonstructural carbohydrates (NSCs) in their stems and grain. Despite keen interest in rice NSC, the dynamics of NSC accumulation, translocation and re-accumulation have not yet been well investigated. AG1 and AG2 QTLs associated with flooding tolerance through catalyzing starch into soluble sugar in germinating seeds. Here we conducted three experiments, greenhouse and field to lay the groundwork for large-scale diversity studies on grain NSC and some agronomic traits under direct-seeded rice (DSR) system, using elite lines incorporating AG1, AG2 and AG1-AG2 QTLs into the popular varieties PSB Rc82 and Ciherang-Sub1 along with the donors Kho Hlan On (AG1) and Ma-Zhan Red (AG2). In germinating seedlings, soluble sugars increased, while starch concentration decreased gradually especially in the tolerant checks and AG1-AG2 introgression lines under flooded soil. Soluble sugar accumulation in stem started to increase from the vegetative stage and peaked at the panicle initiation stage then gradually decreased towards the maturity stage. But Sub1-AG lines had higher sugar and starch concentrations at different growth stages than other genotypes in wet season 2016 and dry season 2017. Plant survival rate was positively correlated with the stem NSC at the early vegetative stage (21 days after sowing), and stem NSC was positively associated with plant height at different growth stages. Among the tested seeding rate, the most suitable seeding rate, 4 g/m² with shallow burial depth (0.5 cm), resulted in better seedling establishment, relatively higher seedling vigor index and higher leaf area index under flooding in DSR system. Introgression of AG1-AG2 QTLs had no any negative impact on nonstructural carbohydrate, germination rate, and growth and biomass production.  相似文献   

A Comparison Investigation on Antioxidant Activities,Physicochemical Properties and Phytochemical Contents of Kiwifruit Genotypes and Cultivars     

Tevfik Ozen  Hamdi Zenginbal  Emine Yazicioglu  Fatih Gul  Ibrahim Demirtas 《International Journal of Fruit Science》2019,19(2):115-135

The present work was conducted to find the physicochemical properties, chemical components and antioxidant capacity of kiwifruit hydroalcoholic extracts (B-31, HO-1-14, J-242, Bruno, Greenlight, Hayward, Monty, Topstar) grown Yalova, Turkey. Phytochemical tests were performed to determine total phenolic, flavonoid and ascorbic acid. Moreover, the kiwifruit extracts were subjected to evaluate their antioxidant potential using different in vitro assays such as total antioxidant, inhibition of lipid peroxidation, reducing power, metal chelating, free radical, H₂O₂ and superoxide anion scavenging activities at 50–500 μg mL^?1, and also found EC₅₀ and IC₅₀ values. The soluble solid content (SSC), titratable acidity (TA) and pH were analyzed in all the genotypes and cultivars. The highest total phenolic, flavonoid and ascorbic acid content were found in J-242 (89.53 mg GAE/100 g), J-242 (109.13 mg QE/100 g) and HO-1-14 (64.15 mg/100 g), respectively. The linoleic, palmitic, stearic and oleic acid methyl esters were detected in all kiwifruit extracts by GC/MS. Significant differences were found between genotypes and cultivars in the antioxidant tests. The extracts showed high inhibition of lipid peroxidation and compared to standards, significantly (p < 0.05). The GC/MS analyses confirmed quantitative variability in the antioxidant profiles. The SSC, TA and pH of kiwifruit genotypes and cultivars showed a moderate level. The key parameters for the most active kiwifruit included the following: total antioxidant activity for B-31, H₂O₂ scavenging activity for J-242 and metal chelating activity for Topstar. In conclusion, this study exhibited the important role played by the genotypes and cultivars background on the chemical content and antioxidant profiles.  相似文献   

Binding profiles and cytokine-inducing effects of fish rhamnose-binding lectins on Burkitt’s lymphoma Raji cells     

Masahiro Hosono  Shigeki Sugawara  Atsushi Matsuda  Takeo Tatsuta  Yasuhiro Koide  Imtiaji Hasan  Yasuhiro Ozeki  Kazuo Nitta 《Fish physiology and biochemistry》2014,40(5):1559-1572

Rhamnose-binding lectin (RBL) is one of the animal lectin categories which take part in the innate immune responses of fish. Osmerus lanceolatus lectin (OLL) from shishamo smelt eggs is an RBL composed of two tandem-repeated domains, both of which are considered to be a carbohydrate-recognition domain. SAL, catfish (Silurus asotus) egg RBL composed of three domains, binds to Burkitt’s lymphoma Raji cells through globotriaosylceramide (Gb3) carbohydrate chain and to reduce cell size and growth by altering membrane composition without causing cell death. In this experiment, we tried to compare the binding effects of these two RBLs on Raji cells. Flow cytometric and fluorescence microscopic analyses revealed that OLL also directly bound to and shrunk Raji cells with ten times less reactivity than SAL but reduced cell growth with decreasing cell viability. Anti-Gb3 antibody completely blocked the binding of SAL to Raji cells but not that of OLL. In addition, the direct bindings of OLL and SAL to Raji cells were comparably inhibited by melibiose, but lactose was more effective inhibitor for the binding of OLL than that of SAL. These results suggest that OLL has slightly different cell-binding property compared with SAL and binds not only to Gb3 but also to the other carbohydrate receptor-bearing β-galactoside chains. The quantitative RT-PCR analysis revealed that SAL induced the expression of TNF-α but not of IFN-γ, IL-1β, and IL-10. Thus, SAL-induced cytostatic effect on Raji cells might be partially caused by TNF-α-mediated signaling pathway.  相似文献   

Factors Determining Consumer Preferences for Pangas and Tilapia Fish in Bangladesh: Consumers’ Perception and Consumption Habit Perspective     

Md. Taj Uddin  Mehedi Hasan Rasel  Mr. Badiuzzaman  Md. Sazedul Hoque 《Journal Of Aquatic Food Product Technology》2019,28(4):438-449

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Effects of storage time and temperature on pH,specific gravity,and crystal formation in urine samples from dogs and cats     

Albasan H  Lulich JP  Osborne CA  Lekcharoensuk C  Ulrich LK  Carpenter KA 《Journal of the American Veterinary Medical Association》2003,222(2):176-179

OBJECTIVE: To determine effects of storage temperature and time on pH and specific gravity of and number and size of crystals in urine samples from dogs and cats. DESIGN: Randomized complete block design. ANIMALS: 31 dogs and 8 cats. PROCEDURE: Aliquots of each urine sample were analyzed within 60 minutes of collection or after storage at room or refrigeration temperatures (20 vs 6 degrees C [68 vs 43 degrees F]) for 6 or 24 hours. RESULTS: Crystals formed in samples from 11 of 39 (28%) animals. Calcium oxalate (CaOx) crystals formed in vitro in samples from 1 cat and 8 dogs. Magnesium ammonium phosphate (MAP) crystals formed in vitro in samples from 2 dogs. Compared with aliquots stored at room temperature, refrigeration increased the number and size of crystals that formed in vitro; however, the increase in number and size of MAP crystals in stored urine samples was not significant. Increased storage time and decreased storage temperature were associated with a significant increase in number of CaOx crystals formed. Greater numbers of crystals formed in urine aliquots stored for 24 hours than in aliquots stored for 6 hours. Storage time and temperature did not have a significant effect on pH or specific gravity. CONCLUSIONS AND CLINICAL RELEVANCE: Urine samples should be analyzed within 60 minutes of collection to minimize temperature- and time-dependent effects on in vitro crystal formation. Presence of crystals observed in stored samples should be validated by reevaluation of fresh urine.  相似文献   

Electron microscopic studies on conjunctiva associated lymphoid tissue (CALT) in Angora goats     

Aşti RN  Kurtdede N  Altunay H  Ozen A 《DTW. Deutsche tier?rztliche Wochenschrift》2000,107(5):196-198

The purpose of this study was to investigate the electron microscopic structure of conjunctiva associated lymphoid tissue (CALT) and to determine the uptake of macromolecules from follicle associated epithelium (FAE) of Angora goats. The sample tissues taken from lower and upper eyelids of ten 5-6 month-old healthy Angora goats were used in this study. The conjunctiva associated lymphoid tissue of Angora goats was formed by solitary or aggregate lymphoid follicle and follicle associated epithelium which cover these follicle. FAE was formed by flattened epithelial cells, lymphocytes, monocytes and polymorph nuclear leukocytes but no goblet cells. Ferritin particles were seen on the apical surface, in the invaginations, vesicles, vacuoles of flattened epithelial cells. It was concluded that these epithelial cells were specialized to uptake macromolecules.  相似文献   

Effect of root applied 28-homobrassinolide on the performance of Lycopersicon esculentum     

B. Ali  S. HayatS. Aiman Hasan  A. Ahmad 《Scientia Horticulturae》2006

The roots of 20 days old seedlings of tomato (Lycopersicon esculentum Mill.) at the time of their transplantation, were dipped in 10⁻⁸, 10⁻⁷, 10⁻⁶ or 10⁻⁵ M of 28-homobrassinolide (HBR) for 15, 30 or 45 min and were allowed to grow in earthen pots, in a net house. The leaves of the plants, at days 30 and 60, possessed elevated quantities of nitrate reductase (NR), carbonic anhydrase (CA) and the contents of chlorophyll. The values for all the above characteristics were significantly higher than that of the water-fed control. The fruits borned at the treated plants were more in number and possessed a lower quantity of ascorbic acid than the control. Moreover, the fruits at ripening, had higher levels of lycopene and β-carotene. Among the treatments, 15 min feeding of 10⁻⁸ M HBR proved best.  相似文献   

Antibacterial activity and cytotoxicity of extractives from Ravenia spectabilis     

Sohrab MH  Chowdhury R  Rahman KM  Hasan CM  Rashid MA 《Fitoterapia》2004,75(5):510-513

A methanolic extract of Ravenia spectabilis, an isolated alkaloid, arborinine plus a fraction comprising arborinine and gamma-fagarine (VLC), showed mild to significant in vitro antibacterial activity. In a brine shrimp lethality bioassay, the extract and the fraction were found to exhibit moderate cytotoxicity having LC50 of 76.26 microg/ml and 14.98 microg/ml, respectively.  相似文献