Effects of three methionine sources in diets on temporal B0AT1 and ASCT2 mRNA expression in different intestinal segments of cobia,Rachycentron canadum     

Yuanfa He  Shuyan Chi  Beiping Tan  Xiaohui Dong  Qihui Yang  Hongyu Liu  Shuang Zhang 《Journal of the World Aquaculture Society》2021,52(1):171-183

A 4‐week feeding trial was conducted using five groups of hybrid grouper (61.15 ± 0.15 g) to explore the potential effects of three methionine (Met) sources. Five isonitrogenous and isolipidic diets were formulated that included a fishmeal (FM) diet; nonsupplemented (NS) diet; or NS diets with the addition of the L‐methionine (L‐Met), DL‐methionine (DL‐Met), or coated Met (Co‐Met) to obtain the same Met level as the FM diet. Fish were randomly distributed into sea cages (30 fish per cage). Weight gain and specific growth rate in the DL‐Met group and FM group were significantly higher than those in all other groups (p < .05). In the proximal and distal intestines, of the 20 gene and time combinations (10 per gene), there were 14 combinations (70%) in which there were no significant differences in gene expression levels between the FM and DL‐Met groups (p > .05). The main reason for the same growth effect between the DL‐Met and FM groups may be attributed to the synchronized absorption at most time points after feeding, which had similar expression patterns of B⁰AT1 and ASCT2 of the proximal and distal intestines between two groups.  相似文献   

何刚德《抚郡农产考略》农业经济思想初探     

张志翔  何红中  伽红凯 《农业考古》2021,(1)

《抚郡农产考略》是清末江西有代表性的农学著作,不仅保存有抚州地区大量农产种类、栽培与加工技术等资料,还包含了大量的商品经济信息。作者何刚德已经认识到自给自足的自然经济会对生产有束缚作用,他在记载各类农产品时,提出“价值”的概念且用货币价格对其衡量,并深入揭示了农产品上市时间、供求、地理、交通条件等因素对价格的影响,进而寻求增值之道。何氏提倡多种经营,大力种植棉花、烟叶等经济作物,发挥柑橘、甘蔗等名优特产优势,促进农业的商品化生产。他还建议通过深加工的方式获取更高利润,并对未能引进西方的先进加工技术而感到惋惜。《抚郡农产考略》体现了中国近代官僚知识分子对农业经济转型的探索,具有重要的历史价值与现实意义。  相似文献   

基于网络药理学分析黄芪防控猪繁殖与呼吸综合征的物质基础及靶点信息     

陈志飞  司广斌  蔡蔚游  梁文清  韦柳明  周霞  贺东生 《中国动物检疫》2021,38(1):69-74

为揭示黄芪防控猪繁殖与呼吸综合征(PRRS)的物质基础及靶点信息,采用网络药理学方法,从中药系统药理学数据库与分析平台(TCMSP)筛选黄芪的潜在活性成分、作用靶点,同时从比较毒理基因组学数据库(CTD)收集PRRS相关宿主基因,最后构建"活性成分-关键靶点"网络、蛋白互作网络,并进行GO功能和KEGG通路富集分析。结果显示,从黄芪筛选出的17个潜在活性成分中,有14个潜在活性成分共计65个基因靶点与PRRS相关宿主基因交叉,其中槲皮素、山奈酚、刺芒柄花素、异鼠李素等潜在活性成分可能对治疗PRRS有重要作用,TNF、IL8、TP53、IL6、IL10、IL1B、JUN等45个蛋白靶点间存在相互作用。此外,黄芪治疗PRRS的作用机制可能涉及细胞外间隙和胞浆等组分,并可能与炎症反应、细胞凋亡、T细胞受体信号通路等生物过程相关。本研究为临床应用黄芪及其提取物治疗PRRS奠定了理论基础。  相似文献   

牦牛源正呼肠孤病毒2型的检测和分离鉴定     

吕珽  陈虹吟  汤承  岳华 《畜牧兽医学报》2021,52(8):2361-2368

旨在调查川西北牦牛哺乳动物正呼肠孤病毒（MRV）的感染情况并分离病毒。采用RT-PCR方法，对采自川西北15个牧场的72份牦牛腹泻粪便样本和其中5个牧场的15份腹泻牦牛血清样本进行MRV检测，阳性样本进一步用分型PCR确定其血清型。结果显示，粪便样本中MRV检出率为20.83%（15/72），血清2型的比例为60%（9/15）；血清样本中MRV检出率为40%（6/15），血清2型的比例为83.33%（5/6）；未检测到其他血清型。成功地从腹泻粪便中分离到1株MRV血清2型毒株（TCID₅₀为4×10^-8.56·mL^-1），并获得长度为23 587 bp的分离株全基因组，该分离株与中国猪源毒株的遗传关系最近；与GenBank中所有的MRV S1基因相比，该分离株有4个独特的氨基酸突变。本研究从牦牛中检测到MRV，并分离到1株牛源MRV血清2型毒株，为进一步研究MRV血清2型生物学特性奠定了基础。  相似文献   

鸡皮刺螨实验室饲养与繁殖技术研究进展     

于荷  王传文  许晓琳  张亚峰  潘保良 《中国家禽》2021,(1):89-94

鸡皮刺螨是一种吸血性外寄生虫,会引起鸡消瘦、贫血,并导致产蛋下降,造成巨大经济损失。为给科研提供可靠虫源,并对杀螨药物、疫苗以及其他防控手段进行效果评价,国内外学者进行了鸡皮刺螨实验室的人工饲养与繁殖技术研究。文章综述了国内外鸡皮刺螨实验室饲养与繁殖技术的研究进展,将其总结归纳为使用动物饲养繁殖技术与离体饲养繁殖技术两类,同时指出了各项技术在应用过程中存在的问题,为该技术的进一步完善与发展提供相关参考。  相似文献   

葡萄风信子物候期观察与观赏性状分析     

吕文涛  娄晓鸣  赵雨菲 《安徽农业科学》2021,49(14):50-53

对荷兰引进的10个葡萄风信子品种进行露地栽培,对其物候期及观赏性状进行观察与评价.结果表明,引进的10个葡萄风信子品种在苏州地区露地栽培均能正常开花;其中"大微笑""幻想创造""蓝魔法""触雪"等品种适宜在园林中推广与应用.  相似文献   

Still Only One Earth. Progress in the 40 years since the First UN Conference on the Environment. By R.E. Hester & R.M. Harrison (eds). Published by Royal Society of Chemistry,Cambridge, 2015. vii + 285 pp. £67.50. Hardback (ISBN 978‐1‐78262‐076‐1).          下载免费PDF全文

R. L. Hough 《Soil Use and Management》2017,33(1):176-176

  相似文献   

Do cover crops change the lability of phosphorus in a clayey subtropical soil under different phosphate fertilizers?          下载免费PDF全文

A. P. B. Teles  M. Rodrigues  W. F. Bejarano Herrera  A. Soltangheisi  L. R. Sartor  P. J. A. Withers  P. S. Pavinato 《Soil Use and Management》2017,33(1):34-44

Plants have developed different mechanisms to absorb and solubilize phosphorus (P) in the soil, especially in environments with low P availability. This study evaluated the effects of different winter cover crops on soil P availability in a clayey subtropical (Hapludox) soil receiving soluble P fertilizer and a rock phosphate applied to the summer crop, under no‐tillage. The experiment was carried out over 3 yrs (2009–2011) with five different cover crop species: common vetch, fodder radish, ryegrass, black oat, white clover and fallow as control. The soil was sampled after the third year of cover crop cultivation and analysed for inorganic and organic P forms according to the well‐established Hedley fractionation procedure. Phosphate fertilizers promoted accumulation of both labile and nonlabile P pools in soil in the near surface layer, especially under rock phosphate. Fertilizer applications were not able to change P fractions in deeper layers, emphasizing that the Brazilian clayey soils are a sink of P from fertilizer and its mobility is almost nil. Although the cover crops recycled a great amount of P in tissue, in a short‐term evaluation (3 yrs) they only changed the content of moderately labile P in soil, indicating that long‐term studies are needed for more conclusive results.  相似文献   

The effect of feeding a novel multistrain yeast fraction on European seabass (Dicentrachus labrax) intestinal health and growth performance     

Mark D. Rawling  Nicola Pontefract  Ana Rodiles  Ilektra Anagnostara  Eric Leclercq  Marion Schiavone  Mathieu Castex  Daniel L. Merrifield 《Journal of the World Aquaculture Society》2019,50(6):1108-1122

Fish were fed a single‐strain yeast fraction (SsYF; 2 g/kg) or a multistrain yeast fraction (MsYF; 0.8 g/kg) for 10 weeks. The results demonstrated significant (p ≤ 0.03) elevations in weight gain, specific growth rate, protein efficiency ratio, and feed conversion ratio in fish fed the yeast fraction‐supplemented diets. In the distal intestine, a significant elevation in microvilli density was observed after 5 and 10 weeks of dietary supplementation with MsYF and SsYF, respectively, compared to control fed fish (p < 0.001). A significant elevation (p = 0.02) in the perimeter ratio was observed in fish fed diets supplemented with the yeast fractions. After 10 weeks of feeding on the experimental diets, Rt‐qPCR demonstrated a significant downregulation (p < 0.05) in the stress response genes, heat‐shock protein 70 (hsp70) and proliferating cell nuclear antigen (pcna), in fish fed diets supplemented with the yeast fractions. Significant (p < 0.05) elevations in interleukin 1‐beta (il1β) and interleukin‐10 (il10) gene expression were observed in fish fed diets supplemented with the MsYF compared to the other dietary groups. These findings suggest that feeding an MsYF specifically at a lower incorporation rate < 1 g/kg, compared to a commercial SsYF at 2 g/kg, is effective in improving the intestinal health status and growth performance of European seabass.  相似文献   

香蕉新品种‘南天红’     

杨兴玉  肖维强  许林兵  李华平  黄秉智  叶春海  陈彪  陈谷  吕庆芳  梁钾贤  吴元立  胡玲玉 《园艺学报》2020,47(Z2):2961-2962

香蕉新品种‘南天红’由‘南天黄’的自然突变选育而成。假茎平均高度2.59 m，幼树假茎外表面暗红色，抽蕾后转绿。雄花苞片外表面常为深红色，花苞卷曲，常不脱落；从种植到收获11 ~ 13个月，单株产量25 kg，品质好。中高抗香蕉枯萎病4号小种，采用配套的栽培技术可在所有香蕉种植区推广。  相似文献