Agrobacterium-mediated transformation of the CrDAT gene and selection of transgenic periwinkle lines with a high vincristine accumulation     

Thi Thanh Nhan Pham  Thi Ngoc Lan Nguyen  Thi Ha Bui  Huu Quan Nguyen  Thi Tam Nguyen  Van Son Le 《The Journal of Horticultural Science and Biotechnology》2019,94(5):591-598

Catharanthus roseus contains vincristine and vinblastine, which are outstanding drugs for cancer. In the biosynthetic pathways of terpenoid indole alkaloids (TIAs) in C. roseus, deacetylvindoline 4-O-acetyltransferase (DAT) is a key enzyme that catalyses the last reaction of vindoline biosynthesis to form vinblastine and vincristine. In this study, the CrDAT transgene was transferred into the periwinkle by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The C. roseus DAT gene was introduced into C. roseus plants and it was confirmed that CrDAT was successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein. Four transgenic periwinkle lines in T1 generation, T1-1, T1-3, T1-6, and T1-7, expressed recombinant DAT protein with the total protein content in the range of 2.86 μg.mg^?1 to 5.12 μg.mg^?1. Moreover, the vincristine contents of four transgenic lines increased by 1.63?2.48-fold compared to non-transgenic plants, ranging from 6.91 µg.g^?1 (fresh weight) to 10.53 µg.g^?1 (fresh weight). The T1-1 line had the highest vincristine content. Hence, the overexpression of the recombinant DAT protein can improve the vincristine accumulation of transgenic C. roseus plants.

Abbreviation: CrDAT - Catharanthus roseus Deacetylvindoline-4-O-Acetyl Transferase; D4H - Deacetoxyvindoline 4-hydroxylase; ELISA - Enzyme-Linked Immunosorbent Assay Monoterpene indole alkaloid; T0, T1 - Generations of transgenic plants; TIAs - Terpenoid indole alkaloids; WT- The wild-type tobacco plants (non transgenic plant); 35S - Cauliflower mosaic virus 35S promoter  相似文献   

Neuroprotective Metabolites from Vietnamese Marine Derived Fungi of Aspergillus and Penicillium Genera     

Elena V. Girich  Anton N. Yurchenko  Olga F. Smetanina  Phan Thi Hoai Trinh  Ngo Thi Duy Ngoc  Mikhail V. Pivkin  Roman S. Popov  Evgeny A. Pislyagin  Ekaterina S. Menchinskaya  Ekaterina A. Chingizova  Shamil S. Afiyatullov  Ekaterina A. Yurchenko 《Marine drugs》2020,18(12)

Low molecular weight secondary metabolites of marine fungi Aspergillus flocculosus, Aspergillus terreus and Penicillium sp. from Van Phong and Nha Trang Bays (Vietnam) were studied and a number of polyketides, bis-indole quinones and terpenoids were isolated. The structures of the isolated compounds were determined by 1D and 2D NMR and HR-ESI-MS techniques. Stereochemistry of some compounds was established based on ECD data. A chemical structure of asterriquinone F (6) was thoroughly described for the first time. Anthraquinone (13) was firstly obtained from a natural source. Neuroprotective influences of the isolated compounds against 6-OHDA, paraquat and rotenone toxicity were investigated. 4-Hydroxyscytalone (1), 4-hydroxy-6-dehydroxyscytalone (2) and demethylcitreoviranol (3) have shown significant increasing of paraquat- and rotenone-treated Neuro-2a cell viability and anti-ROS activity.  相似文献   

Milk vetch dwarf virus infection in the Solanaceae and Caricaceae families in Southeast Asia     

Aamir Lal  Eui-Joon Kil  Vo T. B. Thuy  Chairina Fadhila  Phuong T. Ho  Hee-Seong Byun  Hang T. Dao  Ji-Kwang Kim  Sukchan Lee 《Plant pathology》2020,69(6):1026-1033

Milk vetch dwarf virus (MDV) is an important member of the genus Nanovirus and is transmitted by the aphid Aphis craccivora. MDV has multiple single-stranded DNA genome components, each approximately 1 kb, and two or three alpha-satellite molecules. It mainly infects plants of the legume family Fabaceae. Recently, papaya (Carica papaya) collected in Yesan, South Korea, displaying symptoms of leaf yellowing and dwarfism, was identified as a new host for MDV. To examine the geographical distribution of MDV, papaya samples with symptoms were harvested in South Korea, Vietnam, and Taiwan in August 2018, along with tomato and pepper samples grown in adjacent fields in Vietnam. The results revealed the presence of MDV not only in papaya but also in pepper and tomato. This MDV infection in members of the Solanaceae family was confirmed by Southern blot hybridization performed using a PCR product of segment S as a probe. Based on sequence analysis of three MDV components (M, S, and C3), we verified the presence of three different isolates of MDV in these three countries and homology between sequences of isolates from papaya and from members of the Solanaceae from Vietnam. Taken together, our results clearly demonstrate MDV infection in Vietnam and Taiwan for the first time and confirm that MDV can infect economically important pepper and tomato.  相似文献   

Ouabain-sensitive Na/K-ATPase activity increases during embryogenesis in the giant freshwater prawn Macrobrachium rosenbergii     

Marcy N Wilder  Do Thi Thanh Huong  Atsuro Okuno  Muharijadi Atmomarsono  Wei-Jun Yang 《Fisheries Science》2001,67(1):182-184

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Na/K-ATPase activity during larval development in the giant freshwater prawn Macrobrachium rosenbergii and the effects of salinity on survival rates     

Do Thi  Thanh HUONG  Vidya  JAYASANKAR  Safiah  JASMANI  Hisako  SAIDO-SAKANAKA  Andrew J.  WIGGINTON  Marcy N.  WILDER 《Fisheries Science》2004,70(3):518-520

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Effects of Dietary Supplementation of Barodon on Growth Performance,Innate Immunity and Disease Resistance of Juvenile Olive Flounder,Paralichthys olivaceus,Against Streptococcus iniae     

Chang‐Hoon Shin  Hien Thi Dieu Bui  Samad Rahimnejad  Ji‐Hoon Cha  Byung‐Woo Yoo  Bo‐Kyeun Lee  Hyung‐Jin Ahn  Soo‐Il Choi  Yun‐Jeong Choi  Yong‐Ho Park  Jeong‐Dae Kim  Kyeong‐Jun Lee 《Journal of the World Aquaculture Society》2014,45(3):258-268

This study was conducted to evaluate the effects of dietary supplementation of Barodon, an anionic alkali mineral complex, on growth, feed utilization, humoral innate immunity and disease resistance of olive flounder. A basal experimental diet was used as a control and supplemented with 0.1, 0.2, 0.3, 0.4, or 0.5% Barodon. Triplicate groups of fish (26.4 ± 0.2 g) were fed one of the diets to apparent satiation twice daily for 10 wk. The growth performance was enhanced (P < 0.05) linearly and quadratically in fish fed diets containing Barodon compared with that in fish fed the control. Feed utilization was significantly improved by Barodon supplementation. Serum lysozyme and antiprotease activities were increased quadratically in Barodon fed groups. Also, significantly higher superoxide dismutase activity was found in Barodon‐fed fish. Dietary supplementation of 0.1–0.3% Barodon resulted in significant enhancement of fish disease resistance against Streptococcus iniae. The findings in this study indicate that dietary supplementation of Barodon can enhance growth, feed utilization, innate immunity, and disease resistance of olive flounder and that the optimum level seems to be 0.1% in diets.  相似文献   

Evaluation of smallholder pig production systems in North Vietnam: Pig production management and pig performances   总被引：2，自引：0，他引：2  

U. Lemke  B. Kaufmann  L.T. Thuy  K. Emrich  A. Valle Zrate 《Livestock Science》2006,105(1-3):229-243

This study investigated the productive adaptability of pig breeds under different smallholder production conditions in Vietnam, comparing an indigenous with a Vietnamese improved breed. Fieldwork was conducted in four villages with different remoteness in North Vietnam from 2001 to 2002, in 64 households keeping the improved Mong Cai or indigenous Ban as sow breeds and progeny for fattening. Four visits per farm yielded 234 structured interviews. Reproductive performances were derived from 135 litters. Individual weights of pigs (n = 755) were obtained. Data were analysed by regression, linear and generalised linear, especially loglinear models.

Two distinct pig production systems were identified, that differed in remoteness, market access, resource availability, distribution of pig breeds and pig production intensity. Higher performances of 1.4 and 1.5 litters year^− 1, 8.4 and 8.4 piglets weaned litter^− 1, 66.6 and 93.0 kg piglets weaned sow^− 1 year^− 1, and 136 and 177 g day^− 1 ADG were found in two villages near town with semi-intensive production conditions and a high rate of improved Mong Cai sows and MC and LW × MC offspring in the observed population. Lower performances of 1.1 and 1.1 litters year^− 1, 4.6 and 5.5 piglets weaned litter^− 1, 20.5 and 30.9 kg piglets weaned sow^− 1 year^− 1, and 66 and 85 g day^− 1 ADG were observed in parallel to higher incidences of indigenous Ban pigs away from town under extensive conditions. Total live weight offtake per household per year was higher near town and in one village distant to town. MC and LW × MC remained even under demand-driven conditions below the performance potential reported for improved genotypes. Under resource-driven conditions with a saving-oriented production pattern, the higher-yielding but more demanding Mong Cai might not be an efficient production alternative as a further performance reduction can be assumed. A considerable live weight output was observed under resource-driven conditions and with a higher percentage of crossbred LW × Ban offspring in the population, possibly representing a promising production alternative. Factors influencing the performance expression of pigs, and the suitability of different methods for the assessment of performance parameters on-farm are discussed.  相似文献   

Quantification of DNA of citrus huanglongbing pathogen in diseased leaves using competitive PCR     

Kunimasa Kawabe  Nguyen Thi Ngoc Truc  Bui Thi Ngoc Lan  Le Thi Thu Hong  Masatoshi Onuki 《Journal of General Plant Pathology》2006,72(6):355-359

A quantification system for huanglongbing pathogen using a competitive polymerase chain reaction method and image-analyzing software were developed to obtain precise results. Significant differences in the quantity of pathogen were thus determined in leaves of two citrus cultivars commonly cultivated in southern Vietnam. Less pathogen-related DNA was detected from the tissue of citrus cultivars that are believed to be more tolerant than susceptible cultivars. The quantification system will be used in studies on pathogen proliferation and movement inside citrus tissue.  相似文献   

A new ent-kaurane diterpenoid from Croton tonkinensis leaves     

Minh PT  Ngoc PH  Taylor WC  Cuong NM 《Fitoterapia》2004,75(6):552-556

A new ent-kaurane diterpenoid (1) was isolated from the leaves of Croton tonkinensis. The structure of 1 was determined as ent-7beta-hydroxy-15-oxokaur-16-en-18-ol from spectroscopic methods.  相似文献   

Structural characteristics of cell walls of kenaf (<Emphasis Type="Italic">Hibiscus cannabinus </Emphasis>L.) and fixation of carbon dioxide     

Thi Bach Tuyet Lam  Keko Hori  Kenji Iiyama 《Journal of Wood Science》2003,49(3):255-261

 Kenaf (Hibiscus cannabinus) plants are widely known for their contribution to the global and regional environment because of their ability to fix CO₂. On the other hand, some scientists have doubts about CO₂ fixation by kenaf and have misgivings about the effect of kenaf on the ecosystem. We have characterized the structural characteristics of cell walls of bast fibers, cores, roots, and leaves of kenaf during the maturation of plants and investigated the rate of photosynthesis. During maturation of the kenaf plant the cellulose (bast fiber 52–59%, core 44–46%) and lignin (bast fiber 9.3–13.2%, core 18.3–23.2%) contents increased significantly. The aromatic composition of the lignin of bast fiber was significantly different from that of the core lignin and of other plants. The lignin of bast fiber appears similar to pure syringyl lignin. Fixation of CO₂ by kenaf plants and their contribution to the global environment are discussed. A significatly high rate of photosynthesis of kenaf plants was observed compared to that of woody plants in Japan, but the amount of CO₂ fixation depends on the characteristics of the plantation. If the kenaf was planted in high density, about twice as much CO₂ was fixed as was fixed by trees in a tropical rain forest. Received: April 22, 2002 / Accepted: July 24, 2002 Acknowledgments This project was supported by the Science and Technology Agency (STA) fellowship of the Japan International Science and Technology Exchange Center (JISTEC), which has been successfully applied by Dr. Shuji Hosoya, Forestry and Forest Products Research Institute. We thank Dr. Toshio Sumizono and Mr. Masao Sakurai, Forestry and Forest Products Research Institute, for their kind help in determining the rate of photosynthesis and cultivating the kenaf plants, respectively. We also express our appreciation to Dr. Quang Hung Le, College of Agriculture and Forestry, Ho Chi Minh City, Vietnam for offering information about the cultivation of kenaf at Thu Duc District, Ho Chi Minh City.  相似文献