The intensity of resistance by mature Merino ewes against Haemonchus contortus and Tichostrongylus colubriformis in single-species and combined-species infection     

DB ADAMS  JJ LYNCH†  BH ANDERSON  LR FELL‡  GN HINCH§  RK MUNRO¶ 《Australian veterinary journal》1990,67(12):443-445

Three-year-old, non-lactating and non-pregnant Merino ewes, raised on pasture under a program of strategic treatment with anthelmintic and found to be extremely resistant to "trickle" infection with Haemonchus contortus, were given single-dose infections with either H. contortus or Trichostrongylus colubriformis or both species together. The purpose was to ascertain the intensity of protective immunity against the 2 parasites in sheep with immunity acquired from a presumably slight exposure to infection. To provide a criterion, some infected ewes were immunosuppressed with corticosteroid, dexamethasone. Untreated ewes were extremely resistant to challenge infection with either 15,000 or 150,000 H. contortus or 15,000 T. colubriformis. Surprisingly, when mixed infection was given, egg counts for H. contortus were significantly elevated compared with infection by that species alone. Antibody to antigens from infective larval and adult H. contortus was measured in serum by enzyme-linked-immunosorbent assay (ELISA) during the course of infection. Serum titres against larval antigens were significantly depressed when infections with either H. contortus or T. colubriformis were permitted by immunosuppression with dexamethasone, whereas those against adult antigen were depressed when infection with T. colubriformis was permitted.  相似文献   

Ergovaline does not alter the severity of ryegrass staggers induced by lolitrem B     

SC Finch  JB Vlaming  BL Sutherland  C van Koten  WJ Mace  LR Fletcher 《New Zealand veterinary journal》2018,66(2):93-97

AIM: To investigate a possible interaction between lolitrem B and ergovaline by comparing the incidence and severity of ryegrass staggers in sheep grazing ryegrass (Lolium perenne) containing lolitrem B or ryegrass containing both lolitrem B and ergovaline.

METHODS: Ninety lambs, aged approximately 6 months, were grazed on plots of perennial ryegrass infected with either AR98 endophyte (containing lolitrem B), standard endophyte (containing lolitrem B and ergovaline) or no endophyte, for up to 42 days from 2 February 2010. Ten lambs were grazed on three replicate plots per cultivar. Herbage samples were collected for alkaloid analysis and lambs were scored for ryegrass staggers (scores from 0–5) weekly during the study. Any animal which was scored ≥4 was removed from the study.

RESULTS: Concentrations of lolitrem B did not differ between AR98 and standard endophyte-infected pastures during the study period (p=0.26), and ergovaline was present only in standard endophyte pastures. Ryegrass staggers was observed in sheep grazing both the AR98 and standard endophyte plots, with median scores increasing in the third week of the study. Prior to the end of the 42-day grazing period, 22 and 17 animals were removed from the standard endophyte and AR98 plots, respectively, because their staggers scores were ≥4. The cumulative probability of lambs having scores ≥4 did not differ between animals grazing the two pasture types (p=0.41).

CONCLUSIONS AND CLINICAL RELEVANCE: There was no evidence for ergovaline increasing the severity of ryegrass staggers induced by lolitrem B. In situations where the severity of ryegrass staggers appears to be greater than that predicted on the basis of concentrations of lolitrem B, the presence of other tremorgenic alkaloids should be investigated.  相似文献   

First transgenic trait for control of plant bugs and thrips in cotton     

Waseem Akbar  Anilkumar Gowda  Jeffrey E Ahrens  Jason W Stelzer  Robert S Brown  Scott L Bollman  John T Greenplate  Jeffrey Gore  Angus L Catchot  Gus Lorenz  Scott D Stewart  David L Kerns  Jeremy K Greene  Michael D Toews  David A Herbert  Dominic D Reisig  Gregory A Sword  Peter C Ellsworth  Larry D Godfrey  Thomas L Clark 《Pest management science》2019,75(3):867-877

  相似文献   

The effect of topical tripeptide-copper complex on healing of ischemic open wounds     

Canapp SO  Farese JP  Schultz GS  Gowda S  Ishak AM  Swaim SF  Vangilder J  Lee-Ambrose L  Martin FG 《Veterinary surgery : VS》2003,32(6):515-523

OBJECTIVE: To evaluate the effects of topical glycyl-L-histidyl-L-lysine tripeptide-copper complex (TCC; Iamin 2% Gel; Procyte Corporation, Redmond, WA) on healing in ischemic open wounds. STUDY DESIGN: Experimental study. SAMPLE POPULATION: Twenty-four adult male Sprague-Dawley rats. METHODS: Rats were divided into 3 groups: topical TCC, topical TCC vehicle (hydroxypropyl-methylcellulose), and no treatment (control). Six-mm-diameter, full-thickness wounds were created within an ischemic bipedicle skin flap on the dorsum of each rat. Each day, for 13 days, wound margins were traced, and the TCC and TCC vehicle groups were treated topically. Tracings were scanned, and wound perimeter and area were calculated. On days 6, 10, and 13, selected wounds were biopsied and analyzed for tumor necrosis factor alpha (TNF-alpha) and matrix metalloproteinases (MMP) 2 and 9. RESULTS: A significant decrease in wound area was seen in the TCC group, but not the vehicle group, when compared with the control group on days 3 to 5, 6 to 9, and 11 to 13 and when TCC was compared with TCC vehicle on days 3 and 9. On day 13, initial wound area had decreased by 64.5% in the TCC group, 45.6% in the vehicle group, and 28.2% in the control group. On days 6, 10, and 13, TCC-treated wounds contained significantly lower concentrations of TNF-alpha and MMP-2 and MMP-9 than control wounds. CONCLUSION: Topical TCC resulted in accelerated wound healing in ischemic open wounds. CLINICAL RELEVANCE: Topical TCC is an effective stimulant of healing of ischemic open wounds in rats and may have an application for the treatment of chronic wounds in other species. Clinical evaluation of topical TCC is warranted.  相似文献   

云南省曲靖市1995－2005年兽药质量抽检情况分析     

李芹  尹丽蓉  沈元春 《中国兽药杂志》2006,40(12):55-56,33

以曲靖市兽药监察所最近十年的兽药质量监督抽检报告为基础材料进行统计分析，反映兽药质量监督抽检工作情况及兽药质量状况。  相似文献   

以鸭脚稗秸秆和稻草作为粗饲料对杂交奶牛常量和微量营养素利用和产奶量的影响     

N.k.S.Gowda  王 《江西饲料》2006,(6):28-31

在印度南部,鸭脚稗秸秆和稻草是主要的粗饲料来源。因此用鸭脚稗秸秆和稻草作为粗饲料饲喂杂交奶牛来研究营养利用率、产奶量和牛奶组成。每组日粮用精料、青饲料和粗饲料以30∶45∶25的比例组成(干物质)。在为期50d的消化试验中,每7d对饲料、饲草、粪便、尿液和奶液混合采样,进行常量和微量营养素的分析。鸭脚稗秸秆含有更高的粗蛋白质、钙、磷、镁、铜、锌和钴,稻草含有更高的酸性洗涤纤维、灰分和无水硅酸。饲喂鸭脚稗秸秆的奶牛干物质、粗蛋白质、粗脂肪、中性洗涤纤维、酸性洗涤纤维和大多微量营养素(钙、磷、镁、锌、铁、锰和钴)的摄入量显著增高,并且,饲喂鸭脚稗秸秆的奶牛对干物质、粗蛋白质、粗脂肪、中性洗涤纤维、酸性洗涤纤维的消化率显著增高,钙、铜、锰、钴的肠道吸收率也显著增高。在每组日粮中,微量营养素都能满足奶牛需要量,但稻草组钙含量(0.40%)低于鸭脚稗秸秆组(0.60%)。鸭脚稗秸秆组的平均产奶量(7.0L/头)高于稻草组(6.3L/头)。奶液组成没有差异,仅有乳脂率(4.7%和4.5%),稻草组偏低。上述结果表明鸭脚稗秸秆是一种优于稻草的粗饲料,当稻草作为唯一的粗饲料饲喂奶牛时,需要额外添加钙来满足产奶需要。  相似文献   

人员在岗培训在实施兽药GMP现场管理中的作用     

宋立荣  康孟佼  郑雪花  李晶 《中国兽药杂志》2007,41(9):45-47

论述了对兽药生产企业进行人员在岗培训的必要性,对实施在岗培训的基本程序和应注意的事项进行了总结,旨在为兽药企业进行GMP现场管理提供参考。  相似文献   

印度辣椒产业概况     

Mei-huey Wu  Mubarik Ali  戴雄泽 《辣椒杂志》2009,(2):40-43

辣椒（Capsicum spp．）原产于中南美洲，大约450～500年前，由葡萄牙商人引入印度，并很快在印度大量栽培，成为印度的重要经济作物。2003年印度辣椒种植面积94．55万hm^2，年产鲜椒450万t。印度是辣椒生产大国，也是世界上种植pimento红辣椒最大的国家，面积和产量分别占全球市场份额的46％和44％，单产4．7t／hm^2，低于中围（25．6/hm^2）、  相似文献   

Morphometry,Morphology and Ultrastructure of Ring‐tailed Coati Sperm (Nasua nasua Linnaeus, 1766)          下载免费PDF全文

HVR Silva  FF Magalhães  LR Ribeiro  ALP Souza  CIA Freitas  MF de Oliveira  AR Silva  LDM Silva 《Reproduction in domestic animals》2015,50(6):945-951

The ring‐tailed coati (Nasua nasua) is a procyonid whose population is in sharp decline. Therefore, studies are needed to better understand the reproduction of this animal. For this reason, this study aimed to evaluate the morphology, morphometry and sperm ultrastructure of ring‐tailed coati sperm. Four captive adult males were used for this study. Slides stained with Bengal Rose were used for the morphometric and morphologic analyses. The length and width of the head were measured, as well as the length of the midpiece and tail and the total length of the sperm. Scanning electron microscopy and transmission electron microscopy were used for the ultrastructural analyses. The most obvious morphological abnormalities observed were coiled tails (6.1 ± 8.7%) and the lack of acrosomes (5.4 ± 4.4%). Regarding the morphometry, the measurements of the head (length × width), midpiece (length) and tail (length) were (mean ± SD) 6.2 ± 0.4 × 8.1 ± 0.6 μm, 14.1 ± 0.5 and 63.9 ± 4.1 μm, respectively, and the total length of the sperm was 86.1 ± 4.3 μm. Through electron microscopy, the presence of electron‐lucent points in the nucleus and the presence of approximately 55 mitochondrial spirals in the midpiece were identified. The data obtained in this study provide detailed information on the sperm characteristics of coatis and may inform future research on germplasm conservation, both for this species and other threatened procyonids.  相似文献   

Optimization of Factors Affecting <Emphasis Type="Italic">In vitro</Emphasis> Establishment, <Emphasis Type="Italic">Ex vitro</Emphasis> Rooting and Hardening for Commercial Scale Multiplication of Silk Banana (<Emphasis Type="Italic">Musa</Emphasis> AAB)     

A. A.?WamanEmail author  Pooja?Bohra  B. N.?Sathyanarayana  K.?Umesha  G. K.?Mukunda  T. H.?Ashok  Balakrishna?Gowda 《Erwerbs-Obstbau》2015,57(3):153-164

Factors concerning aseptic culture establishment and hardening were studied in detail in choicest Silk Banana. Effect of size of sucker (small, medium and large), carbon concentration (10, 20 and 30 g/l), season of initiation (wet and dry) and pre-treatments such as segmentation and incision to the explants were studied during initiation. Further, hardening related factors such as substrates used for primary and secondary hardening, nursery nutrition (source and frequency of application) and pre-treatments for ex vitro rooting were also studied. Results revealed that small suckers were most suited for initiations with the least contamination, maximum establishment and higher percentage of greening. Lower concentration of sucrose, though delayed greening, resulted in 100?% establishment of explants. Initiations performed during the drier period were completely free from the fungal contamination and showed less bacterial contamination than those performed during the rainy season. Segmentation of explant into four parts during first subculture supported maximum shoot proliferation by overcoming apical dominance. Coir pith was observed to be the most congenial substrate during primary hardening, whereas coir pith alone or sand: red earth: coir pith (1:1:1) supported superior performance of plantlets during secondary hardening. Single application of mono ammonium phosphate improved growth of plantlets during secondary hardening. Pre-treatment with mono ammonium phosphate gave the best response in terms of rooting and hardening of un-rooted micro-shoots. Thus, the discussed methodology could help the industries to take up commercial scale propagation of Silk Banana.  相似文献