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1.
Sepsis of the calcaneal bursae (CB) presents significant treatment challenges with limited clinical data available in the literature. The objective of this retrospective cohort study was to assess the clinical outcomes associated with CB lavage using either a through-and-through needle or bursoscopic technique. Clinical records of 29 horses treated for septic calcaneal bursitis using either technique between 2005 and 2019 were reviewed. Fisher’s exact test was used to assess statistical significance between first surgical technique and success at first surgery (i.e. not requiring >1 lavage), survival to discharge and return to work (RTW). Bursoscopy was performed in 13/29 (44.8%) cases, and needle lavage in 16/29 (55.2%). In the needle group, 12 (75%) horses were discharged following the first surgery. Four had repeat interventions; two (12.5%) had needle lavage and two (12.5%) had bursoscopy. Of the two horses to have repeat needle lavage, one was subjected to euthanasia and one discharged, and of the two that underwent bursoscopy, one was discharged and one received a third bursoscopy prior to discharge. In the bursoscopy group, seven (53.8%) were discharged and three (23.1%) were subjected to euthanasia following the first surgery. Three (23.1%) received a second bursoscopic lavage with one discharged, one subjected to euthanasia and one having a third bursoscopic lavage prior to discharge. Overall, 18/24 (75%) followed up cases RTW, 10 (55.5%) from the needle group, eight (44.4%) the bursoscopy group. No statistically significant differences between first surgical technique used and success at first surgery (no subsequent lavage(s) required), survival to discharge or return to work were detected. The main limitations of this study are that it is a retrospective study, has a small population with limited statistical power and potential selection bias. No statistically significant differences existed between the outcomes of the two techniques, contrary to the belief that bursoscopic lavage is superior. Larger, multicentred studies, with greater statistical power are required to further assess this relationship.  相似文献   
2.
Phytophthora pluvialis and Phytophthora kernoviae are the causal agents of important needle diseases on Pinus radiata in New Zealand. Little is known about the epidemiology of the diseases, making the development of control strategies challenging. To investigate the seasonality and climatic drivers of sporulation, inoculum traps, consisting of pine fascicles floating on water in plastic containers, were exchanged fortnightly at five sites in P. radiata plantations between February 2012 and December 2014. Sections of needle baits were plated onto selective media and growth of Phytophthora pluvialis and P. kernoviae recorded. To explore the generalizability of these data, they were compared to detection data for both pathogens from the New Zealand Forest Health Database (NZFHDB). Further, equivalent analyses on infection of Rhododendron ponticum by P. kernoviae in Cornwall, UK allowed the comparison of the epidemiology of P. kernoviae across different host systems and environments. In New Zealand, inoculum of P. pluvialis and P. kernoviae was detected between January–December and March–November, respectively. Inoculum of both species peaked in abundance in late winter. The probability of detecting P. pluvialis and P. kernoviae was greater at lower temperatures, while the probability of detecting P. pluvialis also increased during periods of wet weather. Similar patterns were observed in NZFHDB data. However, the seasonal pattern of infection by P. kernoviae in the UK was the opposite of that seen for sporulation in New Zealand. Phytophthora kernoviae was likely limited by warmer and drier summers in New Zealand, but by colder winter weather in the UK. These results emphasize the importance of considering both environmental drivers and thresholds in improving our understanding of pathogen epidemiology.  相似文献   
3.
应用多重PCR检测人工感染鸡呼吸道疾病的研究   总被引:3,自引:0,他引:3  
本文报道了IBV,NDV,ILTV,MG人工感染4周龄SPF鸡和非免疫鸡后,用多重PCR检测试验鸡的咽喉棉拭子和器官组织样品,并与传统的病原分离鉴定,血清学方法进行比较,多重PCR无论是对单一感染病原,还是对两种以上混合感染病原,其敏感性和检测速度都优于传统的鉴别诊断方法,具有较高的实用价值,可以直接应用于临床检测,服务于生产。  相似文献   
4.
介绍16S rRNA基因的特点,阐述其用于病原菌分类鉴定的基本原理,综述其在兽医病原菌分类鉴定中的应用。  相似文献   
5.
近年来,浙江省局部地区国外松纯林受芽流脂病危害较为严重.通过定点现察、分离培养、光镜检查、电镜现察、微量元素测定和用硼砂防治罹病松树等方法,基本查清国外松芽流脂病在浙江省的发生情况,明确了国外松芽流脂病的3种症状顶芽枯死型、丛枝型和枯梢型.国外松芽流脂病是由于松树自身和土壤中的微量元素--有效硼含量不足引起的生理性病害.用喷洒3.0g@kg-1或5.0g@kg-1硼砂溶液防治该病效果显著,防治区病情指数与对照相比平均下降38.5,松树的年平均高生长量也比对照大14.8cm.表4参4  相似文献   
6.
辽宁西部半干旱地区油松纯林改造的研究   总被引:3,自引:0,他引:3  
喀左县位于辽宁西部地区,现存大面积油松人工纯林,不仅改善地力和水土保持效益差,而且火险性强、虫害蔓延成灾、生长早期衰退。为改善其林分生态条件,提高林木生长量和综合效益,在中三家镇对25年生油松人工纯林进行改造试验。结果表明,选择山杏、刺槐、沙棘等乔、灌木树种,利用林间空地、林缘隙地、侵蚀沟壑和疏林地造林,在大面积油松纯林连片地区进行窄带状或块状砍伐后造林,5年后效益显著,林分相对湿度提高了2.2%,土壤有机质含量提高5.4%,土壤含水量提高9.8%,减轻土壤容重3%,林分虫害明显下降,油松树高、胸径生长量分别提高22.6%、19.8%。  相似文献   
7.
马尾松不同苗木类型与整地方式造林早期效果的研究   总被引:3,自引:0,他引:3  
通过对不同苗木类型和整地方式的马尾松幼林的造林成活率、生长状况、生物量和根系特征进行测定,结果表明:容器苗可明显提高造林成活率、当年抽梢高和生物量;整地方式对造林当年的马尾松幼林有一定影响,而在第3年则对幼林树高和抽梢高起着明显促进作用。  相似文献   
8.
樟子松松针锈病病原菌的鉴定   总被引:5,自引:2,他引:3  
1989年在黑龙江省阿城市料甸林场,用樟子松(Pinus sylvestrisvar. mongolica Litv.)松针上的春孢子向健康的黄药(Phelledendron amurenseRupr.)和紫花铁线莲(Clematis fusca var. violacea Maxim)叶片人工接种成功。用人工接种所产生的夏孢子向相同寄主植物接种后,再次发病并产生夏孢子。由此证明阿城市料甸林场樟子松松针锈病菌(Cleosporium clematidis Barcl.)的转主寄主为紫花铁线莲和黄药,病原菌种应为铁线莲鞘锈菌(Coleosporium clema(?)idis Barcl.)。  相似文献   
9.
猴头菌新菌株宁猴6号的选育初报   总被引:2,自引:0,他引:2  
采用多孢分离技术产生多个新组合 ,经驯化筛选获得猴头新菌株宁猴 6号 ,宁猴 6号为适合用针叶林木屑物料栽培的新菌株。生物学特性表明 :猴头菌宁猴 6号菌丝生长适温 2 5~ 2 8℃ ,子实体生长适温 15~2 0℃ ,菌丝生长期短 ,36~ 4 0d长满瓶底 ,栽培袋在常温下培养 4 3~ 5 0d菌丝满袋。室内栽培生物学效率达5 6 .0 0 % ,室外栽培生物学效率达 6 1.4 0 % ,子实体中型匀称近球型 ,柄短或无柄 ,刺长 0 .9~ 2 .2cm。宁猴 6号不仅适于阔叶木屑栽培 ,最突出的是适应针叶林木屑栽培  相似文献   
10.
华山松,赤松次生韧皮部的解剖学观察   总被引:1,自引:0,他引:1  
华山松,赤松次生韧皮部轴向系统由筛胞、韧皮部薄壁细胞及径向片组成,径向系统为射线。径向片和射线中具有蛋白细胞,其特点是细胞核大,细胞质浓,与筛胞之间以单侧筛域相联系。在无功能韧皮部,筛胞和蛋白细胞死亡,被挤毁,射线细胞分裂扩张,并积累后含物。  相似文献   
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