佐剂对鸡补体水平影响的研究     

马本江  王君伟 《黑龙江畜牧兽医》1993,(10):1-3

６０只未免疫的伊莎褐雄鸡雏分成４组，于２４日龄开始用试管法以５０％溶血度为标准样检测补体。３１日时Ａ，Ｂ，Ｃ三组分别皮下注射司盘－８０＋白油，氢氧化铝胶和ＢＣＧ，Ｄ组对照；４６日龄时再次注射。从３１日龄至５６日龄每隔５天检测一次。结果表明，佐剂使鸡的补体水平普遍升高。于每次注射后第５天达到高峰，第１０天左右接近正常。其中ＢＣＧ影响现大，并于５１日龄时天对照组异显著（０．０１＜Ｐ＜０．０５）．  相似文献   

控制sch单养雄蚕品种雌雄蚕孵出比例的催青处理因素研究   总被引：1，自引：1，他引：0  

陈萍  鲁成  朱勇  向仲怀 《蚕业科学》2002,28(1):31-34

研究结果表明 :在RH 6 0 %状态下 ,控制性比的温度和下限时间负相关达到显著水平 ;控制性比的高温处理适于干燥状态下进行 ;处理后雌蚕孵化整齐度明显低于雄蚕孵化整齐度 ,雌雄蚕孵化整齐度均随处理温度的升高而下降 ,尤以雌蚕更为明显。由此提出了在RH 6 0 %状态下sch单养雄蚕品种控制性比的起点温度及温度、时间范围。认为高温催青用下限温度配合 1日收蚁法效果好 ,高温处理以后的胚胎适于在RH 80 %～ 85 %的湿度中保护  相似文献   

Storage of Non-structural Carbohydrates in Sweet Sorghum [Sorghum bicolor (L.) Moench.]: Comparison of Sterile and Fertile Lines     

R. Fortmeier  S. Schubert 《Journal of Agronomy and Crop Science》1995,175(3):189-193

The accumulation of soluble carbohydrates in vegetative parts of sweet sorghum ( Sorghum bicolor (L.) Moench.) represents an intermediate reserve pool for grain filling as well as an irreversible storage up to maturity. In order to study the effect of Sterility on soluble sugar accumulation in vegetative parts, two cytoplasmic male sterile (CMS) lines of sweet sorghum were compared with their corresponding fertile mamtainers for non-structural carbohydrate concentrations in shoots. Plants were harvested at four different physiological stages. The lines showed significant differences in the yield of non-structural carbohydrates. At maturity, the yields of soluble carbohydrates from vegetative parts of the two sterile lines were 93 % and 43 % higher than the yield of the two corresponding fertile lines. At that stage, sucrose represented about 80 % of total soluble carbohydrates and the higher yield of soluble carbohydrates of sterile lines was mainly due to the higher yield of sucrose. This indicates that the yield of extractable carbohydrates, especially sucrose, in vegetative parts of sweet sorghum could be considerably improved by using sterile lines which are utilized in hybrid production.  相似文献   

陆地棉核雄性不育材料在山东不同地区的育性转变及应用研究     

张军  庞居勤 《山东农业科学》1996,(1):4-7

对在山东的济南、平度、莱西、烟台种植的几种陆地棉（Ｇｏｓｓｙｐｉｕｍ　ｈｉｒｓｕｔｕｍＬ．）核雄性不育材料的育性表现进行了研究,结果表明：洞Ａ不育系的衍生材料ＭＢ、ＭＡ在不同气候条件下有育性的变化。在济南高度不育,可作为不育系。在其他几个地区表现不同程度的可育。其中ＭＢ在平度有相当好的育性,能正常结铃。而双隐性核不育材料５９Ａ、６２Ａ未能观察到育性变化。本文就核不育材料在棉花杂交制种中的应用前景及需深入研究的问题作了探讨。  相似文献   

光敏核不育水稻选育与利用的几个问题讨论   总被引：4，自引：0，他引：4  

盛孝邦  丁盛 《杂交水稻》1993,(3):1-3

对现有光敏核不育材料进行了分类,分为４种遗传类型;其中,温光弱感到光敏核不育系是最为理 想的遗传工具,并就其选育途径和选择“粳不籼恢”繁殖制种技术体系进行了论证．  相似文献   

Effect of live weight and dietary supplement of raw potato starch on the levels of skatole, androstenone, testosterone and oestrone sulphate in entire male pigs     

G. Zamaratskaia  J. Babol  K. Andersson 《Livestock Production Science》2005,93(3):235-243

This study evaluated the effect of supplement of raw potato starch (RPS) on the levels of skatole, androstenone, testosterone and oestrone sulphate in plasma from entire male pigs. The study also evaluated relationships between plasma levels of skatole and testicular steroids at three different live weights (LW) of approximately 90, 100 and 115 kg. A total of 111 entire male pigs of a crossbred (Yorkshire dams×Swedish Landrace sires) were used. Animals were raised either in mixed pens, with females and males, or single-sex pens. Each pen contained seven or nine pigs. The most fast-growing three pigs from the pens with nine pigs were slaughtered when they reached 90 kg LW, and the remaining pigs were slaughtered at 115 kg LW. All pigs were fed the same commercial diet until the average pen weight reached 100 kg. Then, 33 out of 80 remaining pigs received RPS, 0.6 kg per pig and day, for 2 weeks prior to slaughter. Blood samples were taken from the pigs at three occasions: first, the day prior to first slaughter occasion, low-weight group; second, the day prior to change in diet, middle-weight group; and third, the day prior to second slaughter occasion, high-weight group. Plasma was analysed for the levels of skatole, androstenone, testosterone and oestrone sulphate. Fat samples were taken at slaughter and analysed for the levels of skatole and androstenone. The levels of skatole and testicular steroids in plasma were significantly higher in entire male pigs from the high-weight group fed no RPS compared to those from low- and middle-weight groups. The levels of the investigated compounds did not differ between low- and middle-weight groups (P>0.1). The diet with RPS induced a decline in skatole levels in plasma and fat (P<0.001), but not plasma levels of testicular steroids and fat levels of androstenone (P>0.05). Skatole levels were positively correlated to testosterone and oestrone sulphate levels in the middle- and high-weight pigs fed no RPS as well as to testosterone in the low-weight group. In the high-weight group fed RPS, skatole levels were not correlated to any of the analysed compounds. Approximately 26% of the entire male pigs (11 out of 43) from the high-weight group fed no RPS produced skatole levels in fat above 0.20 μg/g, whereas the pigs from the low- and high-weight group fed RPS did not produce skatole levels above 0.20 μg/g in fat. Androstenone levels in fat were high in all groups. In total 47% (52 out of 111) pigs expressed androstenone levels above the rejection levels in fat of 1.0 μg/g and 88% (98 out of 111) had androstenone levels above 0.5 μg/g. It was concluded that a lower slaughter weight and the supplement of raw potato starch to the diet could be used to reduce skatole levels in entire male pigs. Androstenone levels in fat, however, could not be reduced by either a lower weight at slaughter or dietary manipulation.  相似文献   

胞质雄性不育性在叶用芥菜上的利用     

Nikornpun  M.　N. Senapa 《中国蔬菜》2004,1(1):10-11

以从美国威斯康星大学引进的叶用芥菜胞质雄性不育系叶用4-4为母本,以泰国清迈大学选育出的8个优良叶用芥菜品种为转育父本,杂交后连续回交4代(BC4),再将BC4与优良叶用芥菜品种40R2-3-4配制8个杂交组合,其中2个组合(4-4 × 19-H-12)×40R2-3-4和(4-4 × 2R2)×40R2-3-4结球率为100％,产量比商用品种分别高34％和13％,比其亲本分别高25％和5％。  相似文献   

水牛精子在胞质内注射后的早期形态变化   总被引：1，自引：0，他引：1  

孟凡丽  韦精卫  石德顺  卢晟盛 《中国兽医学报》2004,24(6):609-612

应用胞质内精子注射 (intracytoplasmic sperm injection,ICSI)技术探讨了水牛卵母细胞胞质与注射精子间的相互作用以及 ICSI精子的形态变化。ICSI后 13h,5 9.4 %的精子头部已膨大 ,且有 18.8%的精子进入解聚状态。雌雄原核发育不同步 ,雄原核在 ICSI后 16 h和 19h的形成率分别为 3.2 %和 4 0 .0 % ;雌原核在 ICSI后 13h已达到 71.9% ,16 h时提高到 90 .3%。经离子霉素与 6 - DMAP联合激活 ,ICSI后 19h产生的胚胎核型以 2 PN PB1 为主 ,其雌原核形成 (激活 )率为 91.3% ,雄原核形成率为 4 0 .0 % ,5 1.3%为孤雌胚。用 5 mm ol/ L 的 DTT预处理精子 1h,可提高精子的解聚率 (30 .9%比 12 .7% ,P<0 .0 5 ) ,但对雄原核的形成率无显著影响 (33.3%比 32 .7% ,P>0 .0 5 )。结果表明 ,水牛精子 ICSI后的雄原核形成时间晚于卵子雌原核形成时间 ,且其比率低于卵子 ,DTT处理精子能提高其解聚率 ,但对雄原核形成无影响  相似文献   

Meiotic competence of mouse oocytes reconstructed by replacing the germinal vesicle with a male pronucleus and somatic nucleus     

Honglin LIU  Fugaku AOKI 《Animal Science Journal》2004,75(2):125-129

The present study examines the contribution of the nucleus to meiotic competence in mouse oocytes that were reconstructed using nuclear transfer. Three types of reconstructed oocytes were produced: MP‐GV, by transplanting the male pronucleus (MP) into germinal vesicle (GV) stage oocytes; 3T3‐GV, by transplanting the nucleus of a National Institute of Health (NIH) 3T3 cell into a GV stage oocyte; and 3T3‐MII, by transplanting the nucleus of an NIH 3T3 cell into a metaphase II (MII) stage oocyte. The fusion rates differed, but not significantly, in the MP‐GV, 3T3‐GV, and 3T3‐MII groups (77, 63, 56%, respectively). Then, meiotic competence was compared in MP‐GV, 3T3‐GV and non‐manipulated GV stage oocytes as a control. Nuclear envelope breakdown occurred in all the reconstructed oocytes, as well as the control ones. The percentage of first polar body extrusion differed between the MP‐GV (100%), 3T3‐GV (72%), and control (67%) groups. DNA staining with Hoechst 33342 revealed that in the MP‐GV‐group oocytes that had reached MII stage, the chromosomes were condensed and aligned in a regular array similar to the normal metaphase plate. By contrast, in 3T3‐GV group oocytes, the condensed chromosomes were irregularly scattered in the cytoplasm. These results suggest that the donor nucleus affects meiotic competence in reconstructed oocytes.  相似文献   

肉苁蓉提取物对雄性小鼠性激素分泌的影响     

马建玲  ;陶大勇 《中兽医医药杂志》2014,(4):43-45

肉苁蓉化学成分复杂,有多种活性作用。本试验利用超声波协助提取、酸提取、醇提取方法提取肉苁蓉生物碱,分别对小鼠进行灌胃给药,观察肉苁蓉提取物对小鼠性激素分泌的影响。结果显示,乙醇提取方法得率最高,肉苁蓉提取物能显著提高小鼠雄激素含量（P〈0.05）。  相似文献