排序方式： 共有54条查询结果，搜索用时 15 毫秒
1.
Thi Thanh Nhan Pham Thi Ngoc Lan Nguyen Thi Ha Bui Huu Quan Nguyen Thi Tam Nguyen Van Son Le 《The Journal of Horticultural Science and Biotechnology》2019,94(5):591-598
Catharanthus roseus contains vincristine and vinblastine, which are outstanding drugs for cancer. In the biosynthetic pathways of terpenoid indole alkaloids (TIAs) in C. roseus, deacetylvindoline 4-O-acetyltransferase (DAT) is a key enzyme that catalyses the last reaction of vindoline biosynthesis to form vinblastine and vincristine. In this study, the CrDAT transgene was transferred into the periwinkle by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The C. roseus DAT gene was introduced into C. roseus plants and it was confirmed that CrDAT was successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein. Four transgenic periwinkle lines in T1 generation, T1-1, T1-3, T1-6, and T1-7, expressed recombinant DAT protein with the total protein content in the range of 2.86 μg.mg?1 to 5.12 μg.mg?1. Moreover, the vincristine contents of four transgenic lines increased by 1.63?2.48-fold compared to non-transgenic plants, ranging from 6.91 µg.g?1 (fresh weight) to 10.53 µg.g?1 (fresh weight). The T1-1 line had the highest vincristine content. Hence, the overexpression of the recombinant DAT protein can improve the vincristine accumulation of transgenic C. roseus plants.Abbreviation: CrDAT - Catharanthus roseus Deacetylvindoline-4-O-Acetyl Transferase; D4H - Deacetoxyvindoline 4-hydroxylase; ELISA - Enzyme-Linked Immunosorbent Assay Monoterpene indole alkaloid; T0, T1 - Generations of transgenic plants; TIAs - Terpenoid indole alkaloids; WT- The wild-type tobacco plants (non transgenic plant); 35S - Cauliflower mosaic virus 35S promoter 相似文献
2.
Seedling crops of gazania, geranium, marigold, mimulus, pansy, verbena and vinca were grown to 60% anthesis in three greenhouse temperature regimes: (a) 26 °C day/6 ± 3 °C night (LT), with a 16.5 °C mean daily temperature (MDT), (b) 26 °C day/17 ± 4 °C night, with a 21 °C MDT, and (c) variable day/night temperatures in response to heating and cooling by a phase change material energy storage module (PCM) with an 8 ± 2 °C night and a 16 °C MDT. Crops bloomed from 2 to 17 days earlier in the 26 °C/17 °C greenhouse. The morphological characteristics of all cultivars of gazania, mimulus, pansy and verbena, geranium and vinca were similar regardless of greenhouse temperature regime. Marigold diploid and triploid cultivars and mimulus were morphologically more variable than the other bedding plant species. Crops grown in the PCM and LT greenhouses had virtually identical morphological characteristics and bloomed in about the same number of days. Plant height was not affected by DIF environments in the greenhouses. The PCM and LT greenhouses had a similar MDT, but the distribution of numbers of hours of mean daily temperatures was different. 相似文献
3.
利用不同稀释倍数的生活污水(污水∶清水,V/V,分别为1∶0;1∶1;1∶2;0∶1)浇灌长春花结果表明,与清水灌溉相比,污灌处理土壤NH4 -N含量由59.01mg/kg提高到64.22~65.89mg/kg,NO3--N含量由2.69mg/kg提高到3.03~4.54mg/kg,速效磷含量由32.29mg/kg提高到37.40~42.41mg/kg;增加了土壤酶活性,其中土壤过氧化氢酶、脲酶及蛋白酶活性分别比对照提高10.76%~23.32%、33.41%~103.69%和31.82%~131.82%;植物根系活性由170.25μg/g.h提高到177.88~471.69μg/g.h,叶绿素含量由1.78mg/dm2提高到2.68~2.98mg/dm2,植物干物质量由11.660g/盆提高到12.130~16.577g/盆。且以稀释1倍污水灌溉处理效果最佳。用原生生活污水直接灌溉虽降低污水肥效,造成土壤Cl-的积累,但对长春花生长及干物质积累无显著影响。 相似文献
4.
5.
6.
在路线踏查的基础上,以长春花资源较丰富的沿海地区为典型调查区域,进一步进行样方调查,对海南归化植物长春花的资源蕴藏量、生长状况和群落特征进行了研究。结果表明,长春花广泛分布于海南沿海各市、县的荒坡及防风林下,适生海拔为0~56 m。样方蕴藏量以文昌湖心村的最高,儋州下浦村的最低。长春花种质资源表型多样性丰富,表型性状变异程度高,变异系数变幅39.97%~93.35%;植株性状Shannon-Weaver多样性指数变幅1.738~2.050。多重比较表明琼海、文昌长春花资源的所有测定指标均无显著差异。但是,除主根长外,这两地的长春花资源的其他指标均显著高于澄迈、临高地区的,且多项指标显著高于儋州地区的。各地样方中多以长春花为优势种,各地的伴生物种虽有差别但并无明显规律。 相似文献
7.
6-BA对长春花幼苗生长的影响 总被引：1，自引：0，他引：1
本试验用50×10-6,100×10-6,200×10-6的6-BA水溶液对长春花幼苗叶片进行喷施处理,以清水处理为对照,观测不同浓度6-BA水溶液对植株高度和叶片生长量的影响。结果表明,用100×1-0 6～200×10-6的6-BA水溶液对长春花幼苗进行喷叶处理对幼苗的生长有较好的促进作用。 相似文献
8.
在温室培养条件下,研究不同体积分数聚乙二醇(polyethylene glycol,PEG)6000处理下长春花幼苗期的胁迫效应以及对文多灵、长春质碱和长春碱等生物碱含量的影响。结果表明:相同体积分数PEG6000处理下,随着胁迫时间的延长,叶片相对含水量呈先降低后上升的趋势,脯氨酸含量呈缓升到急升的趋势。综合二因素的差异显著分析,选择35%(V/V)PEG6000模拟干旱胁迫处理长春花,与对照相比,干旱胁迫的叶片中的过氧化物酶活性先增强后降低;文多灵(vindoline,VIN)含量先升高后下降,长春质碱(catharanthine,CAT)含量先下降后升高,而长春碱(vinblastine,VBL)含量逐渐升高,24 h达到峰值为0.168 0±0.003 6 mg/g（鲜质量）。 相似文献
9.
在温室土培条件下,研究了不同K肥用量对长春花盛花期的生物量、抗氧化物酶活性、丙二醛含量、叶绿素含量及文多灵、长春质碱、长春碱和长春新碱等生物碱含量的影响.结果表明:当外源KCl浓度为100～400 mg/kg时可以有效促进长春花的生长,且超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性随KCl浓度增加而逐渐升高,叶绿素含量也逐渐升高,但叶片丙二醛(MDA)含量则显著下降.当外源KCl浓度达800 mg/kg时,过量的K营养供应抑制了长春花的正常生长.400 mg/kg的施K量可最大限度地促进盛花期长春花的正常生长,并显著促进其生物碱的代谢,使得长春花盛花期文多灵、长春质碱、长春碱和长春新碱含量显著高于其他所有处理,含量分别达42.81、37.82、1.88和3.1 mg/g. 相似文献
10.
研究了细胞质膜钙通道阻断剂氯化镧(LaCl3)、胞内IP3通道阻断剂肝素(Heparin)、胞内CaM活性抑制剂三氟啦嗪(TFP)对长春花光合作用的影响,结果发现:Ca2+通道阻断剂处理后长春花叶片净光合速率(Pn)、PSII最大量子产量(Fv/Fm)、PSⅡ实际量子产量(Yield)、电子传递速率(ETR)、光化学淬灭系数(qP)均下降,非光化学淬灭系数(qN)及Chla、Chlb、Chla+Chlb含量上升,表明Ca2+通道阻断剂对长春花的光合作用有较大影响,且不是通过加速叶片光合色素降解或抑制其合成来实现抑制叶片的光合能力;其中Heparin处理的长春花叶片相关参数变化幅度最大,表明胞内钙库通过IP3通道释放的Ca2+在长春花叶片光合作用过程中发挥了更积极的作用. 相似文献