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为了探索市售冷冻猪产品中金黄色葡萄球菌的污染情况，本研究对2022年从菏泽市牡丹区采集120份的冷冻生猪产品中金黄色葡萄球菌污染状况进行检测分析。结果显示，120份样品中，金黄色葡萄球菌的检出率是21.7%(26/120)。不同类别样品中，纯肉类中金黄色葡萄球菌的检出率为30%，高于排骨类15%和内脏类20%，差异显著（P<0.05）；不同来源样品中，农贸市场检出率最高为30.0%，大型超市中的检出率为13.3%，差异显著（P<0.01）。综上，菏泽市食品中金黄色葡萄球菌污染较为普遍，农贸市场中冷冻猪肉中葡萄球菌的污染情况最为严重，具有潜在的食源性疾病风险。本研究结果可对相关部门进一步加强冷冻猪产品市场监管，采取针对性风险监测预警措施提供参考。 相似文献
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探究乌鲁木齐生牛乳中金黄色葡萄球菌经典肠毒素携带情况及分离株同源性差异，为金黄色葡萄球菌的防控提供参考。对乌鲁木齐地区采集的131份生牛乳样品采用特异性选择培养基结合PCR方法分离鉴定金黄色葡萄球菌，并通过PCR方法检测金黄色葡萄球菌5种经典肠毒素(sea、seb、sec、sed、see)携带情况和根据测序结果对分离株进行同源性分析。结果显示，共分离鉴定到金黄色葡萄球菌13株，总分离率为9.92%(13/131);13株金黄色葡萄球菌经典肠毒素阳性菌株5株，阳性率为38.46%;13株金黄色葡萄球菌的同源性在93.7%～99.7%之间，遗传差异在0.3～6.6之间。研究表明，该地区生牛乳中存在一定金黄色葡萄球菌的污染，经典肠毒素阳性率较高，不同来源的分离株同源性高、差异性小。 相似文献
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《中国奶牛》2021,(5)
为了解上海市生乳中金黄色葡萄球菌污染水平,分析上海市乳制品中金黄色葡萄球菌污染风险来源及乳制品安全情况,本研究对该市乳品加工厂的生乳样本进行了金黄色葡萄球菌计数和细菌分离鉴定,并对分离菌株开展了毒力基因和耐药基因检测。结果显示,84份生乳样品的计数检出率为14.29%,计数范围为20～190CFU/m L,中位数为50CFU/m L。上海市生乳样本金黄色葡萄球菌核酸阳性率38.1%,菌株分离率27.38%。养殖投入品样本中未检出金黄色葡萄球菌。144份养殖环境样本中,仅乳头奶、乳房表面拭子和垫料中检出。分离菌株中肠毒素检出率仅为8.70%,耐甲氧西林金黄色葡萄球菌(MRSA)检测均为阴性。表明上海市生乳中存在一定的金黄色葡萄球菌污染,不宜直接饮用,但该污染水平较低;饲养环节中投入品污染风险较小,饲养环境为乳品中金黄色葡萄球菌污染的主要来源。提示养殖环境控制是防止生乳金黄色葡萄球菌污染、防控奶牛乳房炎的一项重要手段。 相似文献
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3月22日上午8点30分,深圳光明华侨畜禽加工厂一辆载有5000只冰鲜鸡的货柜车从深圳文锦渡口岸驶往香港,这标志着内地的冰鲜、冰冻禽肉产品正式恢复供港。根据国家质检总局和香港食物环境卫生署达成的共识,从3月22日起恢复内地冰鲜、冷冻家禽输港。首批供港的广东省内冰鲜(冻)禽肉加工厂共25家,对应的供港活禽注册养殖场81家。为保证供港禽肉产品的质量安全,国家质检总局表示,除坚持以往严格的检验检疫监管措施外,还增加了两项新要求:一是在活禽出场前5天需批批用荧光PCR检测禽流感病毒,确认无感染H5禽流感病毒再屠宰加工;二是要求一家供港… 相似文献
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李鹏 《中国草食动物科学》2019,(1):52-54
为初步了解并分析酒泉市部分地区羊肉制品中食源性致病菌的污染情况,于2015—2017年对酒泉市5个县市随机采集羊肉制品827份,参照食品国家安全标准对采集的样品中沙门氏菌、金黄色葡萄球菌、大肠杆菌O157:H7、副溶血性孤菌和单核细胞增生李斯特菌污染情况进行了调查。结果显示:共有116份样品被检测出携带食源性致病菌,检出率14.03%,其中金黄色葡萄球菌74份(8.95%)、沙门氏菌23份(2.78%)、副溶血性孤菌12份(1.45%)、单核细胞增生李斯特菌7份(0.85%),没有检出大肠杆菌O157:H7;2015—2017年检出率分别为24.05%、12.09%和7.75%;不同羊肉制品中以油炸肉检出率最高(48.65%),生肉最低(5.67%)。说明酒泉市羊肉制品中食源性病菌污染较为严重,其中主要以沙门氏菌和金黄色葡萄球菌为主,给消费者健康造成潜在的威胁,相关部门需要提高食品安全监管力度。 相似文献
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《中国兽医杂志》2018,(10)
为了掌握金黄色葡萄球菌在西宁地区食品中的分布规律,了解不同来源、不同种类食品中金黄色葡萄球菌的污染状况及其5种肠毒素基因型(A、B、C、D、E)的分布状况。在西宁市地区采集108份食品样品,按食品微生物学(GB 4789.10-2010)金黄色葡萄球菌检验方法结合Baird-Parker氏鉴别培养基筛选金黄色葡萄球菌,通过对金黄色葡萄球菌耐热核酸酶(nuc)基因的PCR扩增以及基质辅助激光解吸电离飞行时间质谱技术(Matrix assisted laser desorption ionization time of flight mass spectrometry,MALDI-TOF-MS)对疑似菌落进行鉴定;同时利用PCR技术对金黄色葡萄球菌的5种肠毒素基因型进行分型检测。经分离及生化鉴定,从108份样品中得到60株疑似金黄色葡萄球菌、PCR方法及MALDI-TOF MS结果,确认为金黄色葡萄球菌,检出率为55. 56%;同时60株金黄色葡萄球菌分别检出A型6株、B型34株和D型2株,共检测到42株,检出率为70%,C型和E型未被检测到。表明西宁市食品中金黄色葡萄球菌的污染处于一个相对较高水平,因食品来源与种类不同,金黄色葡萄球菌的污染情况和毒素基因的分布情况也各不相同,对西宁市食品质量安全控制具有参考意义。 相似文献
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《上海畜牧兽医通讯》2019,(6)
为了解新疆巴州地区金黄色葡萄球菌在奶牛隐性乳腺炎中所起的作用,实验对巴州地区某规模化奶牛场患隐性乳腺炎奶牛的乳样进行金黄色葡萄球菌分离,通过形态学特征观察、生化特性鉴定、16S rRNA鉴定,结果表明此奶牛场隐性乳腺炎的患病率为14.5%,最终确定13株分离菌均为金黄色葡萄球菌,占样本数的7.1%(13/184)。该结果为巴州地区金黄色葡萄球菌性奶牛隐性乳腺炎防治提供了科学依据。 相似文献
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猪胴体淋巴结中金黄色葡萄球菌的分离与鉴定 总被引：1，自引：0，他引：1
随机采取西宁市某生猪屠宰场猪胴体淋巴结60份,进行细菌常规分离培养,结果分离出金黄色葡萄球菌菌株40株,表明该批猪胴体中的金黄色葡萄球菌带菌率可能较高,应引起有关部门的重视。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献