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1.
一种新型鸭瘟病原的分离鉴定及其特性 总被引：2，自引：0，他引：2
从2000年起．山东省的潍坊、临朐、昌乐、昌邑、沂源等饲养肉鸭集中的地区发生一种同鸭瘟症状、剖检变化相似的疾病．但该病主要侵害1月龄以下的雏鸭．成年鸭发病率较低。从自然感染该病典型病死鸭脏器中获得1株病毒，病毒粒子直径80～200nm，呈圆形．有囊膜。进一步试验鉴定，该病毒核酸类型为DNA,ELD50为10^-3.46／0．2mL，对樱桃谷鸭胚、番鸭胚、麻鸭胚及SPF鸡胚的致死率分别为100％、90％、20%和0％。该病毒对氯仿、乙醚、酸碱处理敏感，56℃ 30min能使病毒灭活，该病毒无血凝活性．不能凝集“O”型人、鸡、鸭、鹅、猪、小鼠、豚鼠、绵羊等的红细胞。血清学试验表明，该病毒与鸭肝炎病毒阳性血清、雏番鸭细小病毒阳性血清、小鹅瘟阳性血清之间无中和作用，而能部分中和鸭瘟病毒阳性血清，表明该分离株与传统鸭瘟病毒呈部分相关性。初步确定该病毒为疱疹病毒属疱疹病毒科成员。鉴于该病用传统的鸭瘟疫苗不能预防．故现暂定名为“新型鸭瘟”。 相似文献
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鸭新型疱疹病毒的致病性研究 总被引：1，自引：0，他引：1
本研究分别探讨了F株鸭新型疱疹病毒对番鸭，半番鸭，鹅和SPF鸡的致病性，经实验室人工感染试验表明该株病毒可导致雏番鸭，雏半番鸭发病，死亡，对雏番鸭相同的病变，自致死鸭脏器中重新分离到原攻击病毒；人工感染幸存鸭大多腹泻，生长发育明显受阻，体况消瘦，通过同居感染雏番鸭试验结果表明，该病毒不易经空气传播。对雏鹅，SPF雏鸡的人工感染试验结果可见。该病毒对雏鹅。SPF雏鸡均无致病性。 相似文献
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从辽宁省某鹅场雏鹅体内分离到1株副黏病毒，该分离毒株具有血凝活性且血凝活性能被NDV标准阳性血清和鹅副黏病毒阳性血清所抑制，能使SPF鸡胚、非免疫鸭胚和鹅胚100％死亡；电镜观察见病毒颗粒呈多形性，多为圆形，有囊膜，直径200nm左右；ELD500为10^8.6／mL，MDT为56h，ICPI为1．94。通过RT—PCR扩增出F基因，鉴定为基因Ⅶ型强毒株，测序结果为HBS209，与CH2000的同源率为91．8％，从而确定分离毒株属于禽副黏病毒Ⅰ型(APMV—Ⅰ)的基因变异强毒株。动物接种试验表明，该毒株对鸭无致病性，对鹅、鸡、鸽的致病率和致死率均达100％。 相似文献
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本研究从广东省某发病番鸭场分离得到的呼肠孤病毒(DRV)混合有番鸭细小病毒(MDPV)疫苗株.采用MDPV阳性血清中和后,经2轮番鸭胚有限稀释克隆获得纯化的DRV.电镜观察病毒粒子呈球形、无囊膜、双层衣壳、直径约70 nm.F6代病毒滴度为log107.5 TCID50/0.1 mL,病毒能够致番鸭胚成纤维细胞产生细胞病变,并能够致死番鸭胚和SPF鸡胚,回归1日龄雏番鸭出现典型的肝脏点状或斑块状出血等特征性病变,与临床发病鸭相同.分离株S3基因核苷酸同源性与太湖流域2011年分离株DRV-TH 11最高,为99.9％,与福建番鸭源分离株MDRV-J 18为98％;与早期MDRV在66.8 ％～67.2％之间,处于不同进化分支上.本研究分离株对番鸭胚、SPF鸡胚及雏番鸭致病力强,而且其基因序列比早期分离株变异较大,应加强监测并及时筛选新疫苗株以有效防控该病的发生和蔓延. 相似文献
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从鸭体、禽胚和细胞 3个方面探讨了雏半番鸭呼肠孤病毒 FZ2 株的致病性。经试验表明 ,在实验室条件下 ,该株病毒可导致雏番鸭、雏半番鸭发病、死亡 ,对雏番鸭的致死率为 14 .3%～ 4 1.7% ,对雏半番鸭的致死率高达 38.5 % ,且死亡鸭表现出与自然感染呼肠孤病毒病死的雏番鸭、雏半番鸭相同的病变 ;人工感染幸存鸭大多生长发育明显受阻。该株病毒经尿囊腔途径接种 ,对番鸭胚、半番鸭胚、北京鸭胚、SPF鸡胚的致死率分别为 10 0 %、96 %、2 8%和 0 ,致死鸭胚的肝脏、脾脏表面见白色坏死点。经蛋传试验表明 ,该株病毒有可能经胚蛋垂直传染。以该株病毒在番鸭胚成纤维细胞 (MDEF)上连续传接 10代 ,结果细胞病变 (CPE)仍不明显 ,表明其不易适应 MDEF。由此可见 ,该株雏半番鸭源呼肠孤病毒具有较强的致病力 相似文献
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从洛江区临床发病鸭分离到2株病毒,2株分离病毒能够致死鸡胚,能被Ⅰ型鸭病毒性肝炎标准强毒高免血清特异性中和。分离毒株在鸡胚上传代培养,并测定ELD50分别为10-6.32/0.2 m L、10-5.49/0.2 m L;经动物回归试验,对1日龄雏番鸭的致死率分别为80%和70%,雏番鸭出现明显的角弓反张姿态,剖检可见肝脏出血斑、出血点,为鸭病毒性肝炎的典型症状,表明分离到的病毒为Ⅰ型鸭病毒性肝炎病毒(DHV-I)。 相似文献
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根据基因库中鸭Ⅰ型肝炎病毒和番鸭细小病毒的基因序列,设计2对特异性引物和2条用不同荧光基团标记的TaqMan探针.对反应条件和试剂浓度进行优化,建立能够同时检测鸭Ⅰ型肝炎病毒和番鸭细小病毒的二重荧光定量RT-PCR方法.该方法敏感性好,对鸭Ⅰ型肝炎病毒和番鸭细小病毒的检测敏感性均达到200个模板拷贝数;该方法特异性强,对鸭圆环病毒、鹅细小病毒、鸭副黏病毒、鸭瘟病毒和禽流感病毒等病原体的检测全为阴性.本研究建立的鸭Ⅰ型肝炎病毒和番鸭细小病毒的二重荧光定量RT-PCR方法,具有快速、敏感、特异、定量和重复性好等优点,可用于临床上鸭Ⅰ型肝炎病毒和番鸭细小病毒感染的检测. 相似文献
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番鸭"三周病"、细小病毒型"白点病"和小鹅瘟是雏番鸭常见的三种细小病毒病,对番鸭养殖业危害较为严重。本文对从广东惠州及周边地区病死番鸭中分离鉴定的1株番鸭细小病毒型"白点病"病毒、1株小鹅瘟病毒和1株番鸭"三周病"病毒分别感染健康雏番鸭,将发病的临床症状、病理学变化进行对比,从而为基层兽医工作者提供三种番鸭细小病毒病临床鉴别、初步诊断更加直观的依据。 相似文献
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鸭“白点病”（暂定名）病原学研究 总被引：11，自引：1，他引：10
对3个“白点病”（暂定名）发病严重的鸭场的病死鸭进行细菌学检查均为阴性，但均分离到病毒。对其中1株病毒进行了鉴定。负染及超薄切征电镜观察，可见呈球形或卵圆形、直径80-230nm、有囊膜的病毒。经理化特性、生物学特性及核酸类型测定，确定该病毒为疱疹病毒科成员。血清中和试验表明，该病毒与鸭瘟病毒、鸭疱疹病毒Ⅱ型无血清学相关性，故暂定名为鸭疱疹病毒Ⅲ型。人工感染试验复制出与自然感染病死鸭相同的病变，初步证明该病毒为鸭“白点病”病原。 相似文献
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将抗番鸭GPV单抗腹水采用透析法标记异硫氰酸荧光素（FITC）,制备成抗GPV荧光抗体,研制检测GPV抗原的直接免疫荧光诊断方法。结果显示GPV荧光抗体仅与GPV阳性的组织切片或细胞呈现特异性荧光,与番鸭细小病毒（MPV）、番鸭呼肠孤病毒（MDRV）、鸭副粘病毒（DPMV）、鸭病毒性肝炎病毒（DHV）和正常番鸭组织切片不反应;与间接荧光方法的符合率为92．9％。表明GPV荧光抗体具有较好的特异性、敏感性和准确性,可用于临床快速诊断番鸭小鹅瘟病。 相似文献
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The study was conducted to establish the duplex Real-time PCR assay for detecting both duck tembusu virus (DTMUV) and duck plague virus (DPV). According to the sequences of DTMUV E gene and DPV UL6 gene in GenBank, two sets of specific oligonucleotide primers for DTMUV and DPV along with two TaqMan probes were designed. The duplex Real-time PCR assay was developed through optimization of reaction conditions and validation of specificity, sensitivity and repetitiveness of the method. The sensitivity of the assay were both 100 template copies for DTMUV and DPV. There was no specific bands of the same sizes were amplified from other duck pathogens, such as duck Newcastle disease virus, duck hepatitis virus, muscovy duck parvovirus, duck circovirus, H9 subtype avian influenza virus, egg drop syndrome virus. This duplex Real-time RT-PCR assay is a sensitive, quick, specific and quantitative test for detection of DTMUV and DPV, and will be useful for the control of these viruses in ducks. 相似文献
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番鸭新病——花肝病的研究：Ⅰ．分离毒的致病必及感染途径试验 总被引：4，自引：2，他引：2
番鸭花肝的是近年流行的一种新的番鸭疫病，其特征性病变是死亡番鸭的肝、脾、小肠等部位出现灰白色的坏死点，本文自广东省主要疫区分离到几株病毒，这些病毒在番鸭胚上可继代繁殖，其胚液可使番鸭妇病。不同途径感染试验表明该病毒通过肌肉注射、爪垫部注射、口服，同居感染均可使番鸭发病和死亡，并具有典型病变，分离毒不能使半番鸭，本地鸭，鸡发病。 相似文献
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Kaleta EF Kuczka A Kühnhold A Bunzenthal C Bönner BM Hanka K Redmann T Yilmaz A 《DTW. Deutsche tier?rztliche Wochenschrift》2007,114(1):3-11
The continuing westward spread of avian influenza A virus of the subtype H5N1 in free-living and domestic birds forced the European Union and the German federal government to enhance all biosecurity measures including in-house keeping of all captive birds from October 20 to December 15, 2005. Movement of captive ducks and geese of many different species from a free-range system to tight enclosures and maintenance for prolonged times in such overcrowded sheds resulted in pronounced disturbance of natural behaviour, interruption of mating and breeding activities and possibly additional stress. Under these conditions the birds developed signs of severe disease and enhanced mortality twentyfour days later. A total of 17 out of 124 (14%) adult birds and 149 out of 184 year-old birds (81 %) died during the outbreak. A herpesvirus was isolated from many organs of succumbed ducks and geese that was identified as a duck plague herpesvirus by cross neutralization test using known antisera against duck plague virus. The published host range of duck plague comprises 34 species within the order Anseriformes. We report here on additional 14 species of this order that were found to be susceptible to duck plague virus. The exact source of the herpesvirus could not identified. However, low antibody titres in some ducks at day of vaccination indicate that at least some of the birds were latently infected with a duck plague herpesvirus. The remaining healthy appearing birds were subcutaneously vaccinated with a modified live duck plague vaccine (Intervet, Boxmeer, NL) that stopped losses and resulted in seroconversion in most of the vaccinated birds. 相似文献
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番鸭细小病毒与鸭圆环病毒二重PCR方法的建立 总被引：1，自引：0，他引：1
根据基因库中鸭圆环病毒和番鸭细小病毒的基因序列,分别设计了两对特异性引物,通过对二重PCR扩增条件的优化,研究建立了可同时鉴别检测鸭圆环病毒和番鸭细小病毒的二重PCR方法。用该方法对同一样品中鸭圆环病毒和番鸭细小病毒的模板进行PCR扩增,结果均得到了与实验设计相符的351bp（鸭圆环病毒）和474bp（番鸭细小病毒）的扩增条带,而对鸭Ⅰ型肝炎病毒、鹅细小病毒、鸭副黏病毒、鸭瘟病毒和禽流感病毒等病原体的检测全为阴性。敏感性测定结果表明：该二重PCR技术最低能检出100fg的鸭圆环病毒和番鸭细小病毒DNA模板。研究建立的鸭圆环病毒和番鸭细小病毒的二重PCR方法,具有快速、敏感、特异、定量和重复性好等优点,可用于临床上鸭圆环病毒和番鸭细小病毒感染的检测。 相似文献
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V ronique Marius-Jestin Martine Cherbonnel J. P. Picault G. Bennejean 《Comparative immunology, microbiology and infectious diseases》1987,10(3-4):173-186
This paper describes the isolation and identification of a duck plague virus (DP) and a paramyxovirus (PMV6), from the livers and intestines collected in 4-month old mule ducks, under fattening, exhibiting 75% mortality and necrotic-haemorrhagic gross lesions. These viruses were isolated in specific pathogen free (SPF) muscovy duck eggs and SPF chicken eggs respectively. Then the DP virus was adapted to duck and chicken fibroblasts. The disease was reproduced in 2-week old SPF muscovy ducklings, intramuscularly inoculated with the previous organs, as well as in contact ducks. From them, only the DP virus was isolated again. Experimentally the intramuscular inoculation of the duck plague French vaccinal strain, 4 h post contact, did not prevent the disease and did not decrease its severity.
Regarding the DP virus, the typical signs and lesions observed in experimentally infected muscovy ducks as well as the presence of intranuclear inclusions of the epithelial cells of their oesophagus, intestines, bursa of Fabricus and liver on the one hand, and on the other hand, of the epithelial cells of the duck egg chorio-allantoïc membrane and fibroblasts inoculated with the samples first defined, allowed the characterization of the virus. Direct electron microscopy, as well as the results of seroneutralization tests with different specific avian Herpes virus antisera confirmed the DP virus identification. Moreover the DP isolate was not antigenically different from the serotype actually known.
The haemagglutinating virus (PMV6) was characterized by direct electron microscopy as well as with 18 specific avian Myxovirus antisera; its identification was confirmed too by the specific seroconversion observed 4 weeks post-inoculation of this virus, in 11 weeks old SPF muscovy ducklings.
Finally an assay was carried out to appreciate the pathogenicity of theses viruses inoculated either separately or associated. It showed the high pathogenicity of the DP strain. The PMV6 was apathogenic and no synergic effect with the DP virus was demonstrated. It appears to be the first isolation of PMV6 in France, to our knowledge. The epidemiological circumstances related to theses isolations are discussed. The failure of the emergency vaccination in contact ducks, might be attributed to the high virulence of the DP strain. 相似文献
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Several muscovy ducks from a free-roaming flock of 65 muscovy and mallard ducks died over a 3-week period. Three muscovy ducks were necropsied. Gross and microscopic changes were compatible with duck virus enteritis, and the virus was isolated. In addition to intranuclear viral inclusion bodies in several tissues, intracytoplasmic inclusion bodies were present in esophageal and cloacal epithelium. By electron microscopy, the membrane-bound intracytoplasmic inclusions were found to contain enveloped herpesvirus, and nuclei contained herpes viral nucleocapsids. 相似文献
20.
R E Gough 《The Veterinary record》1984,114(11):262-265
From 1977 to 1983 the Central Veterinary Laboratory, Weybridge confirmed 19 outbreaks of duck virus enteritis in the United Kingdom. All the outbreaks involved collections of captive waterfowl and there were no reported cases in commercial ducks. In many instances the disease was associated with contact with migrating waterfowl, particularly male mallards (Anas platyrhynchos). Muscovy ducks (Cairina moschata) and related species appeared to be particularly susceptible. The most sensitive system for isolating the virus was muscovy duck embryo tissue cultures. The duckling inoculation test was found to be the most reliable method of confirming the disease. 相似文献