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丝尾鳠的驯养及人工繁殖初报 总被引：1，自引：0，他引：1
丝尾鳠(Mystus wyckioides)是分布于云南省境内澜沧江水系的土著鱼类,近年来由于滥捕及澜沧江流域修建电站等原因,丝尾鳠野生资源日益稀缺,其市场经济价值和研究推广价值也随之提高.2005年起,在澜沧江的一级支流中采集野生亲本,并进行了驯养及人工繁殖研究.于2006年4月首次获得人工繁殖成功,至2009年已累计繁殖成功并培育出5cm以上苗种70余万尾.经过4年的技术改进,2009年催产6批、28组,催产率达到100%,授精率达到71%,孵化率达到88%.介绍了丝尾鳠从采集野生亲鱼到成功驯养的全过程,分析了采集、驯养过程中存在的问题及解决方法.在成功驯养的基础上,探讨了野生丝尾鳠的人工催产繁殖及孵化技术. 相似文献
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泥鳅人工繁殖试验报告 总被引：5，自引：0，他引：5
闽北地区,4月上旬当水温达到16℃以上时,即可进行泥鳅人工繁殖。试验期催产雌鳅289尾.获产213尾,在水温19—28℃条件下,效应时间为8.5—17h,平均催产率为73.7%,产卵数60.47万粒,平均受精率76.1%,得苗27.21万尾,孵化率为58.6%。文章还就进一步提高泥鳅人工繁殖的“三率”等问题进行了初步探讨。 相似文献
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翘嘴鳜人工繁殖及苗种培育试验 总被引：1，自引：0，他引：1
2012年5月开展了翘嘴鳜人工繁殖和苗种培育试验,催产10组,催产率100%,获受精卵65万粒,受精率85%,出苗52万尾,孵化率80%,育成乌仔36万尾,夏花22万尾,成活率42.3%。 相似文献
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泥鳅养殖技术之三大刺鳅人工繁殖研究初报 总被引：1，自引：0，他引：1
大刺鳅(Mastacembelus armatus)在分类学上隶属于鲈形目、刺鳅科、刺鳅属,俗称蛇鱼、刀鳅、镰刀鱼,为小型野生淡水鱼类,我国长江以南各水系均有分布(云南省内主要分布于澜沧江、怒江、元江各水系),但产量甚微。为了保护和开发利用这一名贵鱼种,云南省水产研究所从2001年开始实施“云南野生珍稀鱼虾人工驯化养殖研究”课题,大刺鳅为其中一个品种,并于2004年开始利用已驯化的大刺鳅进行人工繁殖试验,经过2年中的10余次试验至2005年6月10日首次催产成功,以后又相继2次催产获成功,人工孵化出鱼苗1850尾,经过20天~35天的培育,获体长3.8cm~7.0cm的幼鱼1016尾,为今后扩大繁殖规模和推广养殖打下了基础。 相似文献
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为了在安徽开展中华倒刺鲃人工繁殖技术研究,于2010年从湖北引进中华倒刺鲃苗种,培育至性成熟后,于2016年5月20日和6月8日分两批进行人工催产和授精试验,共催产雌鱼40尾,雄鱼31尾,之后将受精卵放入孵化缸和孵化环道中进行流水孵化。结果表明:全长5 cm左右的中华倒刺鲃苗种,在安徽经过6年池塘培育后可用于人工繁殖,催产率为90.91%~94.44%,受精率为92%~96%,孵化率在80%以上。仔鱼出膜后6~7 d即可下塘培育,两批共下塘鱼苗66万尾。 相似文献
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匙吻鲟亲鱼培育及规模化人工繁殖技术 总被引：1，自引：0，他引：1
采用分阶段培育方式培育匙吻鲟亲鱼,后备亲鱼（5～6龄）在水库及坝下池塘培育,催产用亲鱼在流水池中强化培育,各阶段通过控制水流速度、水温、水质和饵料条件,满足其性腺发育需求。2008年至2010年共培育后备亲鱼1017尾,性腺发育成熟亲鱼110组,并逐尾包埋电子标签,建立不同年龄段亲鱼种源库。采用B超结合体形观察判断早期性别,结合挖卵检查准确判断亲鱼的成熟度,依据成熟度确定催产剂量,并通过控制水温、水流速度、挤卵排精时间等催产和孵化条件,人工繁殖的催产率、受精率和孵化率显著提高,年均分别达到95%、95%、78.3%,三年共孵化匙吻鲟鱼苗1246.6万尾。实现了匙吻鲟规模化人工繁殖的目标。 相似文献
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为研究异育银鲫"中科3号"规模化人工繁殖技术,用该鱼作母本,建鲤作父本,进行了两批次的人工催产及人工授精试验。试验结果:第一批次催产雌亲鱼500尾,雄亲鱼40尾,在水温18.2℃时,催产率74.2%,受精率63.5%,孵化率83.6%;第二批次催产雌亲鱼900尾,雄亲鱼90尾,在水温21.5℃时,催产率95.8%,受精率86.2%,孵化率85.2%。两批次共获得异育银鲫"中科3号"水花1000余万尾。结果表明,亲鱼的成熟度和催产水温对催产率均有一定影响,催产水温在20~22℃时效果较好。 相似文献
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乌苏里拟鲿人工繁育技术初探 总被引：1，自引：0，他引：1
为研究乌苏里拟鲿的人工繁殖和苗种培育技术,2011年5月和6月从吉林省鸭绿江地区采购两批亲鱼运回安徽进行了繁育试验。注射人工催产激素促熟催产,人工授精,水泥池流水孵化,土池施肥培水并用粉料培育鱼苗。试验结果:第一批次催产雌鱼260尾、雄鱼56尾,水温22.0～22.5℃,产卵率为35%,平均受精率55%;孵化水温19.5～23.0℃,因感染水霉,仅获得300多尾鱼苗。第二批次催产雌鱼275尾、雄鱼60尾,水温24.0～25.5℃,产卵率50%,平均受精率65%;孵化水温24.5～28.3℃,由于孵化期间使用亚甲基蓝防治水霉,孵化率达到75%。两个批次共繁殖鱼苗19万尾,培育出全长4.5～5.5cm的鱼种6万尾,培育成活率31.58%。 相似文献
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Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean 
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下载免费PDF全文 The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna. 相似文献
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Liping Li Rui Wang Wanwen Liang Xi Gan Ting Huang Yan Huang Jian Li Yunliang Shi Honglin Luo Ming Chen 《广西水产科技》2014,(1)
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China. 相似文献
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Iciar Martinez Amaya Velasco Ricardo Pérez-Martín Eskil Forås Michiaki Yamashita Carmen G. Sotelo 《Journal Of Aquatic Food Product Technology》2013,22(4):289-297
The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus. 相似文献
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In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg. 相似文献
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Growth hormone (GH) and reproduction: a review 总被引：5，自引：0，他引：5
Florence Le Gac Odile Blaise Alex Fostier Pierre-Yves Le Bail Maurice Loir Brigitte Mourot Claudine Weil 《Fish physiology and biochemistry》1993,11(1-6):219-232
Interaction between growth and reproduction occurs in many vertebrates and is particularly obvious at certain stages of the life cycle in fish. Endocrine interactions between the gonadotropic axis and the somatotropic axis are described, the potential role of GH being emphasised. A comparative analysis of these phenomena in mammals, amphibians and fish, suggests a specific role of GH in the physiology of puberty, gametogenesis and fertility. It also shows the original contribution made by studies on the fish model in this field of investigations. 相似文献
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Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation. 相似文献
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Controlled infection of Poecilia reticulata Peters (guppy) with Tetrahymena by immersion and intraperitoneal injection 下载免费PDF全文
下载免费PDF全文 G Sharon M Pimenta‐Leibowitz M C L Vilchis N Isakov D Zilberg 《Journal of fish diseases》2015,38(1):67-74
Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality). 相似文献
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