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由于喀什地区近几年使用新疆天康畜牧生物技术股份有限公司生产的口蹄疫Asial-O二价灭活疫苗免疫牛时产生较大的疫苗过敏反应,并出现少量牛死亡的现象,极大地影响了喀什地区口蹄疫防疫工作的开展;并依据我们对全区口蹄疫免疫牛血清的抽样监测显示:牛免疫抗体水平普遍不高,甚至出现个别地域牛群体免疫抗体水平达不到要求的实情,为此,我们进行了下列试验. 相似文献
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为掌握新疆莎车县不同养殖方式下牛O型口蹄疫免疫水平,科学指导各乡镇进一步提高免疫质量,采用LPB-ELISA检测方法对全县2021年上半年牛O型口蹄疫免疫抗体进行了检测。结果显示,县规模养殖和散养方式的牛O型口蹄疫免疫抗体合格率分别为80.43%、82.17%,均达到了农业农村部所要求的70%标准,被检31个乡镇的规模养殖和散养方式的牛O型口蹄疫免疫抗体达标率分别为87.10%、77.42%。研究表明,新疆莎车县牛O型口蹄疫免疫质量总体良好,免疫工作开展扎实有效,但个别乡镇的免疫水平不高,仍需加强免疫。 相似文献
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为掌握伊吾县牛羊口蹄疫免疫抗体水平,采用液相阻断ELISA抗体检测(LPB-ELISA)方法,收集6个乡镇2019—2021年抽检春秋两季牛1258头、羊2496只,进行时间、地域分布描述。实验显示,口蹄疫A型总体合格率90.86%、90.91%,O型口蹄疫免疫抗体总体合格率94.12%、94.03%,免疫抗体效价大于70%。不同区域前山乡口蹄疫整体免疫水平最高,吐葫芦乡牛A型口蹄疫免疫抗体较低仅为74.67%。结果表明,全县牛羊口蹄疫免疫抗体检测效果整体良好,部分乡镇免疫较低。需做好基层牛羊口蹄疫免疫调研,查找原因,提出了今后的工作方向,加强对防疫员开展从疫苗保存、免疫部位选择、免疫剂量技术指导,特别是动物检疫站监管,为伊吾县畜牧业高质量健康发展提供依据。 相似文献
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为及时了解口蹄疫免疫抗体水平，采取科学防疫措施，确保疫苗免疫效果，我们在2008年春季防疫督查考核中，在人户抽查免疫密度的同时，随机采集牛、羊、猪血清572份进行了口蹄疫免疫抗体水平检测。现将检测结果报道如下： 相似文献
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疫苗免疫是预防和控制家畜口蹄疫最有效的方法和途径。我市严格按照农业部免疫方案的要求,全面开展了口蹄疫强制免疫工作,免疫密度达到100％,对预防和控制口蹄疫起到了重要作用。为及时了解口蹄疫免疫抗体水平,采取科学防疫措施,确保疫苗免疫效果,我们在2008年春季防疫督查考核中,在入户抽查免疫密度的同时,随机采集牛、羊、猪血清572份进行了口蹄疫免疫抗体水平检测。现将检测结果报道如下： 相似文献
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《养殖与饲料．饲料世界》2020,(6)
本研究对辽宁阜新地区春季集中免疫期间猪、牛、羊口蹄疫免疫抗体采用ELISA法进行监测,同时对猪、牛、羊进行口蹄疫病毒非结构蛋白抗体单抗阻断ELISA检测和口蹄疫病毒荧光RT-PCR检测,将免疫抗体监测与病毒检测结果进行综合系统分析,有效评估该地区口蹄疫预防免疫效果和感染带毒情况,及时掌握口蹄疫防控工作情况,做出风险评估,进行风险交流,发挥预警预报作用。结果表明,在口蹄疫春季集中免疫后,阜新地区猪、牛、羊O型口蹄疫平均免疫抗体合格率为86.97%,牛、羊亚洲Ⅰ型口蹄疫平均免疫抗体合格率为86.74%,奶牛A型口蹄疫平均免疫抗体合格率为95.62%,均超过了国家农业农村部对重大动物疫病评价的总体要求(有效免疫抗体合格率要达到70%以上)。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献