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精子形态学检测是预测精子功能最有价值的指标之一,形态异常精子越多受精率越低。用4种伊红-苯胺黑活体染色法(Blom、Bakst、Morisson、Jaskowski)评价三黄鸡和贵妃鸡的精子形态,结果表明:染色方法显著影响精子形态,但不影响精子活率,染液对精子自然形态的保持至关重要。Blom染色法导致精子头部膨胀,畸形精子显著增多(P≤0.01),Bakst、Morisson和Jaskowski染色法均可作为评定家禽精子形态的实用方法,其中Jaskowski染色方法最简便经济,值得在家禽生产中推广应用。 相似文献
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倪俊娟 《河南畜牧兽医(综合版)》2014,(4):23-24
精子畸形率为评定猪精液品质的重要指标,精子畸形率≤20％才合格.精子畸形率过高将直接影响到猪人工授精的受胎率.笔者通过总结姬姆萨染色制片过程中的经验体会,提出了能够提高精子染色效果的方法,通过提高制片染色质量提高精子畸形率检查的准确度,能够减少由于制片染色不当造成的误判. 相似文献
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猪精子形态学检查的染色法很多,但在实际操作中比较复杂,不易应用,本人在泰国学习时,SRISVWAN教授推荐Eosin-Nigrosin苯胺曙红染色法,更便于生产实际操作。 相似文献
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精液品质检测中，精液的存活率、精子畸形率、顶体完整率是判定精液品质优劣的常用指标。在以往检测过程中，通常采用常规染色法，即每测一个指标时进行一种染色操作，这样操作过程繁琐、耗时、耗力、耗材。本试验旨在对不同染色方法进行比较，从中寻找出一种操作简便、染色效果较佳的染色方法，这样即可省去许多步骤，也可减少多次实验操作造成误差。１材料与方法１．１精液样本：中国荷斯坦牛颗粒冻精（山西省种牛站）。１．２染色原理：利用活精子对特定染料不着色而死精子着色的特点来区分死精子和活精子，从而得出存活率指标。通过油镜… 相似文献
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就精子加热法、伊红-苯胺黑染色法、中性红染色法3种精子活率的测定方法进行比较,以便找到一种快速又准确的方法。通过测定肉羊精液,结果表明,用以上3种方法测定精子活率,差异不显著。但在试验过程中发现,伊红染液浓度对精子活率有影响。中性红染色法能在光学显微镜下对精子进行形态描述与识别。 相似文献
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<正> 前言 顶体反应是精子受精过程的重要步骤,也是估测精子受精能力的重要指标。本文就是从这个角度出发,采用TST三重染色法染色黑白花奶牛不同公牛个体解冻后的精子,观察精子的顶体反应率,同时测定了精子的活力,顶体完整率和畸形率,以及精清中钙的含量和乳酸脱氢酶(LDH)活性,从而来探讨TST三重染色法是否可作为评定精液品质的可靠方法。 相似文献
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采用不同浓度梯度的中性红、龙胆紫及尼罗蓝染液对长白公猪精子染色,根据染色现象及精子活率,探究3种染料较为理想的作用时间和浓度。结果:0.01%中性红染色2 min后活精子着红色,死亡精子不着色;0.05%龙胆紫染色1 min后活精子着淡蓝色,死亡精子深紫色;0.1%尼罗蓝染色2 min后活精子着淡蓝色,死亡精子不着色。3种情况下精子的存活率与空白对照组比较无显著差异(P0.05)。试验表明,以上3种染色条件能清晰地区分存活精子和死亡精子,可用于测定精子活率。 相似文献
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黄党池 《江西畜牧兽医杂志》2007,(5):13-13
<正>精子活率是评价精液品质的一项重要指标,其在光学显微镜下的测定,操作简便、直观,仍为临床常规检查所应用。本试验就精子用伊红-苯胺黑染色法和中性红染色法2种精子活率的测定方法进行比较,以便选择一种快速又准确的方法。 相似文献
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高晓霞 《上海畜牧兽医通讯》2014,(4):65-65
<正>精子活率的检测是精液品质检测的一项重要内容,活率的高低直接反映了精子质量和精子的生存能力,精子活率的降低将明显影响精子的受精能力。伊红-苯胺黑染色法和中性红染色法计算精子活率,其区别在于伊红Y为酸性染料,带有阴离子,可与带有阳离子的物质结合。死精子的细胞膜受到损伤,能渗透到精子内部与阳离子结合而呈红色;活精子细胞膜完整,伊红Y不能渗入细胞内而不着色。而中性红为水溶性染料,能通过活精子的 相似文献
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为了探讨每剂含不同前进运动精子数(≥1 000万个和≥2 000万个)和稀释精液不同平衡时间(1 h、2 h、3.5 h)对藏獒冻精品质的影响,试验比较了0.25 m L细管剂型藏獒冻精解冻后精子活力、精子顶体完整率、精子畸形率、精子37℃存活时间。结果表明:每剂含前进精子数≥2 000万个与≥1 000万个相比,藏獒冻精解冻精子活力、精子顶体完整率、精子畸形率差异不显著(P0.05);而每剂含前进运动精子≥2 000万个组的藏獒冻精解冻后37℃存活时间优于每剂含前进运动精子数≥1 000万个组,且差异显著(P0.05)。藏獒稀释精液平衡2 h组的冻精活力显著高于平衡1 h组和3.5 h组(P0.05),而平衡1 h组和3.5 h组间差异不显著(P0.05)。冻精顶体完整率以平衡2 h组最高,为(41.00±1.47)%,与平衡3.5 h组间存在显著差异(P0.05)。平衡2 h组的冻精畸形率低于平衡1 h组和3.5 h组。说明藏獒0.25 m L细管剂型以每剂含前进运动精子数≥2 000万个,稀释精液平衡时间以2 h为宜,可获得较好的冷冻效果。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献