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1.
单链特异性(SSS)核酸酶是基因组学研究的有效工具.本研究对绿豆芽核酸酶、S1和芹菜汁提取物三种SSS核酸酶检测桉树ESTP检测的酶切条件进行了优化,优化因子依次包括Mg2 浓度、酶切温度、酶用量和/或pH值等因子.三种酶的最适酶切温度均为60℃.绿豆芽核酸酶优化后的酶切反应体系为:10×Buffer(pn 5.56)1.0 μL(NaAc 300 mmol/L,Nacl 1.0 mol/L,ZnAc2 10 mmol/L,甘油50%,MgCl2 100 mmol/L),PCR产物5 μL,酶4.5 U,超纯水补至10 μL;S1的优化酶切体系为:10×Buffer(pH 5.46～3.67)1.0 μL(MgCl2100 mmol/L,ZnAc2 2 mmol/L,Bis-Tris 200 mmol/L,Triton X100 0.02%,BSA 0.002 mg/mL),PCR产物5 μL,S1酶5 U,超纯水补至10 μL;CJE的优化酶切体系为:10×Buffer(pH 7.5)1.0 μL(MgCl2 100 mmol/L,Hepes100 mmol/L,KCI 100 mmol/L,Triton X100 0.02%,BSA 0.002 mg/mL),PCR产物5.0 μL,CJE酶1.0 μL,超纯水3.0 μL.综合考虑酶切效果和成本,推荐S1为检测桉树ESTP的经济、有效的SSS核酸酶. 相似文献
2.
氯霉素单克隆抗体免疫金标快速检测试纸的研制及性能测定 总被引：10，自引：0，他引：10
以分泌抗氯霉素单克隆抗体(CAP mAb)杂交瘤细胞株的建立为基础,应用胶体金免疫层析试验(GICA)成功研制出CAP残留快速检测试纸(CAP_Strip),并对其性能进行了测定。结果表明,CAP_Strip的标准曲线呈典型的S型,符合4参数logit曲线拟合,目测检测限为0.5μg/L;与氯霉素琥珀酸钠的交叉反应为160%,与其他酰胺醇类和抗菌药物无交叉反应;其敏感性与竞争ELISA试剂盒相当,符合率为100%;10 min内显示结果,具有快速、敏感、特异、简便等特点,适合CAP残留快速检测的推广应用。 相似文献
3.
链霉素金标单克隆抗体快速检测试纸的研制及其特性鉴定 总被引：1，自引：0，他引：1
建立了分泌抗链霉素单克隆抗体(STR mAb)杂交瘤细胞株并生产了STR mAb,采用胶体金免疫层析试验(GICA)技术成功研制出STR残留快速检测试纸(STR-Strip),并对其性能进行了鉴定.结果显示,目测检测限为12.5 μg/kg;与氯霉素等药物无交叉反应;其敏感性与竞争ELISA试剂盒相当,符合率为100%.研制的快速检测试纸可以在10 min内显示结果,具有快速、敏感、特异、简便等特点,适合STR残留的现场快速检测. 相似文献
4.
采用HPLC-MS/MS技术,同时测定土壤中的矮壮素和缩节胺残留。土壤样品经缓冲溶液提取后,经Agilent ZORBAX Rx-Sil(100 mm×3.0 mm,1.8μm)型色谱柱分离,以含10 mmol/L NH4CH2COOH+0.2%HCOOH的水溶液和甲醇(体积比60∶40)为流动相进行等度洗脱,经正离子多反应监测模式测定目标化合物。结果表明,2种化合物在质量浓度1.0~50.0μg/L线性关系良好,定量限(LOQ)矮壮素为0.8μg/L,缩节胺为0.6μg/L;加标回收率平均为97.6%~99.5%;相对标准偏差(RSD)平均为2.5%~2.9%,适用于检测土壤中矮壮素和缩节胺。 相似文献
5.
目的 建立容舒口服液单味药材的HPLC指纹图谱,并建立成品口服液HPLC检测方法。方法 对不同产地的口服液原药材酸枣仁、当归、五味子和玫瑰进行HPLC指纹图谱评价。建立以槲皮素和山奈素为指标的容舒口服液的HPLC检测方法,色谱条件：Welch Ultimate XB-C18 (4.6×250 mm, 5 μm);甲醇：0.4%磷酸溶液(40:60),360nm; 1mL.min-1; 40℃;10 μL。结果 不同产地的主药材的HPLC指纹图谱峰形区分度较高,共有峰相似度高于98.2%,可以显著区别不同产地药材的含量差异。成品HPLC检测方法建立成功,检测指标槲皮素(5.79-38.6 μg.mL-1,r=0.9999)和山奈素(3.68-24.55 μg.mL-1,r=0.9999)在规定范围内线性关系良好;平均加样回收率分别为100.89%和99.86%,RSD值分别为1.96%(n=6)和1.39%(n=6);结论 通过指纹图谱法和HPLC法控制容舒口服液的质量,结果准确,方法稳定可行。 相似文献
6.
采用HPLC-MS/MS技术,同时测定番茄苗中的矮壮素和缩节胺残留。样品经水提取后,把提取液和乙腈(体积比1∶1)混合,沉淀净化,再经Agilent ZORBAX Rx-Sil(100 mm×3.0 mm,1.8μm)色谱柱分离,以含浓度10 mmol/L NH4Ac和体积分数0.2%HCOOH的水溶液与甲醇(体积比60∶40)为流动相进行等度洗脱,经电喷雾正离子(ESI)+电离及多反应监测(MRM)模式来测定目标化合物,采用基质标准溶液曲线法进行定量。结果表明,2种化合物质量浓度在1.0~50.0μg/L内线性关系良好,定量限(LOQ)分别为0.4μg/L和0.2μg/L,加标回收率平均为95%~105%,相对标准偏差(RSD)平均为2.5%~6.5%,可满足番茄苗中矮壮素和缩节胺残留的检测要求。 相似文献
7.
为探索适宜棉花的SSR-PCR反应体系,采用L9(34)正交实验设计,研究了棉花SSR-PCR反应体系的主要成分对扩增结果的影响.试验结果表明,棉花SSR-PCR最适反应体系为:在20 μL反应体系中,TaqDNA聚合酶用量为1 U、dNTP浓度0.2 mmol/L、引物浓度0.6 μmol/L、Mg2 浓度1.0 mmol/L和模板DNA50 ng;引物退火温度为55℃～58℃.利用六种棉花材料和10条不同引物验证此反应体系,8%聚丙烯酰胺凝胶电泳结果显示,扩增结果在100～300 bp,反应体系的稳定性和可重复性较好. 相似文献
8.
建立了高效液相色谱—串联质谱法(LC/MS/MS)测定羊肉中残留的6种磺胺类药物:磺胺喹噁啉(sulfaquinoxaline,SQX)、磺胺嘧啶(sulfadiazine,SDZ)、磺胺二甲氧嘧啶(sulfadimethoxine,SDM)、磺胺间甲氧嘧啶(sulfamonomethoxin,SMM)、磺胺对甲氧嘧啶(sulfameter,SMT)、磺胺甲噁唑(sulfamethoxazole,SMZ)。样品经乙腈匀浆提取,正己烷脱脂后旋转蒸发至近干,氮气吹干,流动相溶解并定容,再用正己烷净化后上机检测,在多反应监测模式下检测,外标法定量。结果表明,6种磺胺质量浓度在10～500μg/L内线性关系良好,相关系数R2为0.998 1～0.999 8,回收率为78%～105%,定量限为10μg/kg。 相似文献
9.
采用HPLC-MS/MS技术,同时测定土壤中的矮壮素和缩节胺残留。土壤样品经缓冲溶液提取后,经Agilent ZORBAX Rx-Sil（100 mm×3.0 mm,1.8μm）型色谱柱分离,以含10 mmol/L NH4CH2COOH+0.2% HCOOH的水溶液和甲醇（体积比60∶40）为流动相进行等度洗脱,经正离子多反应监测模式测定目标化合物。结果表明,2种化合物在质量浓度1.0~50.0μg/L线性关系良好,定量限（LOQ）矮壮素为0.8μg/L,缩节胺为0.6μg/L;加标回收率平均为97.6%~99.5%;相对标准偏差（RSD）平均为2.5%~2.9%,适用于检测土壤中矮壮素和缩节胺。 相似文献
10.
固相萃取-反相高效液相色谱法测定大鼠血清中的甘氨胆酸 总被引：6，自引：0，他引：6
通过建立血清中甘氨胆酸浓度的测定方法,旨在为观察甘氨胆酸在大鼠血清中浓度的变化规律做试验依据,为开发有价值的动物源性药物做前期探索.试验采用固相萃取-反相高效液相色谱法(SPE-HPLC),色谱柱为Waters Xterra RP18(150 mm×4.6 mm,5 μm),流动相为乙腈-KH2PO4溶液,检测波长213 nm,柱温30℃,进样体积10 μL.结果在0.156 25～10.0 μg/mL范围内甘氨胆酸浓度与峰面积呈良好的线性关系,回归方程Y= 39 579X+57 823,R2=0.998 4;以信噪比S/N=3为标准,最低检测浓度为0.02 μg/mL,平均回收率98.49%;日内RSD 0.52%～2.00%,日间RSD 0.74%～4.98%.该方法具有简便、稳定、灵敏、准确、实用等特点,适用于血清中甘氨胆酸含量的测定分析. 相似文献
11.
Jens Jensen 《Euphytica》1979,28(1):47-56
Summary The high-lysine gene in Risø mutant 1508 conditions an increased lysine content in the endosperm via a changed protein composition, a decreased seed size, and several other characters of the seed. The designation lys3a, lys3b, and lys3c, is proposed for the allelic high-lysine genes in three Risø mutants, nos 1508, 18, and 19. Linkage studies with translocations locate the lys3 locus in the centromere region of chromosome 7. A linkage study involving the loci lys3 and ddt (resistance to DDT) together with the marker loci fs (fragile stem), s (short rachilla hairs), and r (smooth awn) show that the order of the five loci on chromosome 7 from the long to the short chromosome arm is r, s, fs, lys3, ddt. The distance from locus r to locus ddt is about 100 centimorgans. 相似文献
12.
G. H. Kroon 《Euphytica》1994,76(1-2):125-125
Summary
K x vadensis is a hybrid of K. blossfeldiana and K. marmorata obtained after doubling the number of chromosomes. 相似文献
13.
Hongyan WEI Jian ZHOU Jinguang LU Jingzheng SONG Qiongxi YU Zhihong JIANG 《Medicinal Plant》2019,(6):27-29
[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenol... 相似文献
14.
Summary Avoidance of rust fungi that was based on poor appressorium induction was previously found in Hordeum chilense. In the present study 95 accessions of Triticeae were screened for avoidance of Puccinia hordei. The percentage of appressorium formation per germinated spore ranged from 6 to 90%. On none of the 41 accessions of Aegilops, Agropyron, Elymus, Secale, Thinopyrum or Triticum studied was the rate of appressorium formation lower than 25%. Lower rates of appressorium formation were, however, found on accessions of wild barley species Hordeum brachyantherum, H. marinum, H. parodii and H. secalinum. Its implications in cereal breeding are discussed. 相似文献
15.
D. A. Bond G. J. Jellis G. G. Rowland J. Le Guen L. D. Robertson S. A. Khalil L. Li-Juan 《Euphytica》1993,73(1-2):151-166
Progress is being made, mainly by ICARDA but also elsewhere, in breeding for resistance to Botrytis, AScochyta, Uromyces, and Orobanche; and some lines have resistance to more than one pathogen. The strategy is to extend multiple resistance but also to seek new and durable forms of resistance. Internationally coordinated programs are needed to maintain the momentum of this work.Tolerance of abiotic stresses leads to types suited to dry or cold environments rather than broad adaptability, but in this cross-pollinated species, the more hybrid vigor expressed by a cultivar, the more it is likely to tolerate various stresses. 相似文献
16.
Chunli TANG Fengxian ZHAO Hongxia CHEN Jiabao MA Jiangcun WEI Meiyan QIU Zhen XIE 《Medicinal Plant》2019,(6):60-65
[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination... 相似文献
17.
Mohammed Riyazaddin Polavarapu B. Kavi Kishor Are Ashok Kumar Belum V. S. Reddy Rajendra S. Munghate Hari C. Sharma 《Plant Breeding》2015,134(4):423-436
Sorghum shoot fly, Atherigona soccata, is one of the important pests of postrainy season sorghums. Of the 90 sorghum genotypes evaluated for resistance to this pest, RHRB 12, ICSV 713, 25026, 93046 and 25027, IS 33844‐5, Giddi Maldandi and RVRT 3 exhibited resistance in postrainy season, while ICSB 463, Phule Anuradha, RHRB 19, Parbhani Moti, ICSV 705, PS 35805, IS 5480, 5622, 17726, 18368 and 34722, RVRT 1, ICSR 93031 and Dagidi Solapur showed resistance in rainy season, suggesting season‐specific expression of resistance to A. soccata. ICSB 461, ICSB 463, Phule Yasodha, M 35‐1, ICSV 700, 711, 25010, 25019 and 93089, IS 18662, Phule Vasudha, IS 18551 and 33844‐5 and Barsizoot had fewer deadhearts than plants with eggs across seasons, suggesting antibiosis as one of the resistance mechanism. Five genotypes exhibited resistance with high grain yield across seasons. Correlation, path and stepwise regression analyses indicated that leaf glossiness, seedling vigour, trichome density, oviposition and leaf sheath pigmentation were associated with the expression of resistance/susceptibility to shoot fly, and these can be used as marker traits to select and develop shoot fly‐resistant sorghums. 相似文献
18.
E. Keep 《Euphytica》1986,35(3):843-855
Summary Cytoplasmic male sterility (cms) is described in the F1 hybrids Ribes × carrierei (R. glutinosum albidum × R. nigrum) and R. sanguineum × R. nigrum. In backcrosses to R. nigrum, progenies with R. glutinosum cytoplasm were either all male sterile, or segregated for full male fertility (F) and complete (S) and partial (I) male sterility. Ratios of F:I+S suggested that two linked genes controlled cms, F plants being dominant for one (Rf
1) and recessive for the other (Rf
2).Segregation for cms in relation to three linded genes, Ce (resistance to the gall mite, Cecidophyopsis ribes), Sph
3(resistance to American gooseberry mildew, Sphaerotheca mors-uvae) and Lf
1(one of two dominant additive genes controlling early season leafing out) indicated that Rf
1and Rf
2were in this linkage group. The gene order and approximate crossover values appeared to be: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXafv3ySLgzGmvETj2BSbqef0uAJj3BZ9Mz0bYu% H52CGmvzYLMzaerbd9wDYLwzYbItLDharqqr1ngBPrgifHhDYfgasa% acOqpw0xe9v8qqaqFD0xXdHaVhbbf9v8qqaqFr0xc9pk0xbba9q8Wq% Ffea0-yr0RYxir-Jbba9q8aq0-yq-He9q8qqQ8frFve9Fve9Ff0dme% aabaqaciGacaGaamqadaabaeaafaaakeaacaWGdbGaamyzamaamaaa% baGaaiiiaiaacccacaGGWaGaaiOlaiaacgdacaGG0aGaaiiiaiaacc% caaaGaaiiiaiaacccacaGGGaGaamOuaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaaccdacaGGUaGaaiOmaiaacs% dacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaaaacaWGsbGaamOzaSGa% aGOmaOWaaWaaaeaacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccaaaGaamitaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccacaGGGaaaaiaadofacaWGWbGaamiAaSGa% aG4maaaa!6E4D!\[Ce\underline { 0.14 } Rf1\underline { 0.24 } Rf2\underline { } Lf1\underline { } Sph3\]. Crossover values of 0.36 for Ce-Lf
1, and 0.15 for Lf
1-Sph
3were estimated from the relative mean differences in season of leafing out between seedlings dominant and recessive for Ce and Sph
3.It is suggested that competitive disadvantage of lf
1-carrying gametes and/or zygotes at low temperatures may be implicated in the almost invariable deficit of plants dominant for the closely linked mildew resistance allele Sph
3. Poor performance of lf
1- (and possibly lf
2-) carrying gametes and young zygotes during periods of low temperature at flowering might also account for the liability of some late season cultivars and selections to premature fruit drop (running off). 相似文献
19.
[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and ortho... 相似文献
20.
Summary Apple and pear pollen was irradiated with doses of 0, 50, 100, 250 and 500 krad (gamma rays) and stored at 4°C and 0–10% r.h. From the in-vitro germination percentages an average LD 50 dose of about 220 krad was estimated. For both irradiated and untreated pollen a close and corresponding lineair relationship existed between germination percentage and pollen tube growth.Irradiated pollen was much more sensitive to dry storage conditions than untreated pollen, resulting in less germination and more bursting. Apparently, irradiation caused the pollen cell membrane to lose its flexibility faster than normal. Rehydration of dry-stored, irradiated pollen in water-saturated air restored germination percentages up to their initial levels. The importance of this procedure in germination trials is stressed. 相似文献