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[目的]了解奶牛子宫内膜炎乳房链球菌的耐药性和携带毒力基因。[方法]采集香河县5家奶牛场患子宫内膜炎奶牛的子宫黏液样本76份,用PCR鉴定分离菌株的乳房链球菌,采用微量肉汤稀释法测定8种抗菌药对乳房链球菌的最小抑菌浓度（MIC）,判断其耐药性。用PCR检测乳房链球菌携带的10种毒力基因。[结果]PCR检测出18株乳房链球菌,总分离率23.68%。18株乳房链球菌对克林霉素、庆大霉素、四环素耐药性较高,耐药率分别为66.67%、88.89%、72.22%。所有菌株均携带slp、sua、gapC、fpb、cfu和pauA,而hasA、hasB、acdA检出率分别为38.89%、50.00%、33.33%,未检出hasC。[结论]该地区乳房链球菌是奶牛子宫内膜炎主要致病菌之一,耐药性较严重,毒力基因流行广泛,应加强监测,合理用药。 相似文献
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为探讨奶牛子宫内膜炎和隐性乳房炎之间的相关性,在宁夏地区3个大型奶牛场(根据临床型子宫内膜炎的诊断标准)选择子宫内膜炎患牛111头,用兰州隐性乳房炎检测法检测(LMT)乳样,其中82.5%的病牛同时患有隐性乳房炎,然后进行了子宫样和乳样的采集,并用常规细菌分离、培养结合高智能全自动细菌鉴定及药敏分析系统(VITEK 2 Campact),确定引起宁夏奶牛子宫内膜炎的病原菌有18种110株,引起乳房炎的病原菌有23种107株,其中大肠杆菌、停乳链球菌、克氏库克菌、金黄色葡萄球菌、假单胞杆菌、乳房链球菌、产色葡萄球菌、蜡样芽孢杆菌、荧光假单胞杆菌、产酸克雷伯菌、副溶血弧菌、克雷伯氏菌可以同时引起奶牛子宫内膜炎和隐性乳房炎。 相似文献
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奶牛子宫内膜炎大肠杆菌的分离血清型鉴定及其耐药性测定 总被引：1，自引：0，他引：1
子宫内膜炎是奶牛常发的一种生殖系统疾病,不仅影响机体正常的生理功能,而且会导致患牛长期不孕,有的甚至造成终生不孕[1].据调查,我国奶牛子宫内膜炎的平均发病率约为20%,在奶牛不孕症中,由子宫内膜炎所致的不孕奶牛约占60.1%～92.0%[2].奶牛子宫内膜炎主要由分娩或产后子宫感染病原微生物引起,其中以大肠杆菌、葡萄球菌、链球菌等感染最为常见.临床上多使用抗菌药物治疗奶牛子宫内膜炎,随着病原菌耐药性的不断加重,用药剂量随之增加,这不仅提高了治疗成本,而且增加了乳产品中药物残留的风险.本文对内蒙古地区引起奶牛子宫内膜炎的大肠杆菌进行了分离、血清型鉴定,并测定其对24种抗菌药物的敏感性,旨在探明内蒙古地区奶牛子宫内膜炎大肠杆菌的血清型及耐药水平,为临床防治该病提供科学依据. 相似文献
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重组溶葡萄球菌酶对奶牛乳房炎和子宫内膜炎常见病原菌的体外杀菌试验 总被引：1，自引：1，他引：1
从患乳房炎和子宫内膜炎奶牛的奶或子宫黏液中分离菌株,鉴定后选取金黄色葡萄球菌105株、表皮葡萄球菌20株、大肠埃希氏菌20株、链球菌38株(其中停乳链球菌8株,无乳链球菌30株)和牛源多杀巴氏杆菌5株,在体外用重组溶葡萄球菌酶、苯唑西林、氨苄西林、庆大霉素按照微量肉汤稀释法进行了杀菌试验。结果表明,重组溶葡萄球菌酶对奶牛乳房炎和子宫内膜炎分离的金黄色葡萄球菌和表皮葡萄球菌具有良好抑杀效果,对链球菌(包括无乳链球菌和停乳链球菌)和牛源多杀巴氏杆菌具有一定的抑杀效果,对大肠杆菌作用较差;苯唑西林、氨苄西林、庆大霉素都有一定量的耐药菌株产生,而重组溶葡萄球菌酶对苯唑西林、氨苄西林、庆大霉素耐药菌株同样具有良好抑杀效果。 相似文献
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从患乳房炎和子宫内膜炎奶牛的奶或子宫黏液中分离菌株后经鉴定选取金黄色葡萄球菌105株、表皮葡萄球菌20株、大肠埃希氏菌20株、链球菌38株（其中停乳链球菌8株，无乳链球菌30株）和牛多杀性巴氏杆菌5株。在体外用重组溶葡萄球菌酶、苯唑西林、氨苄西林、庆大霉素按照微量肉汤稀释法进行抑杀菌实验。结果表明：重组溶葡萄球菌酶对奶牛乳房炎和子宫内膜炎分离的金黄色葡萄球菌和表皮葡萄球菌（包括耐药菌株和敏感菌株）具有良好抑杀效果，对链球菌（包括无乳链球菌和停乳链球菌）和牛多杀性巴氏杆菌具有一定的抑杀效果，对大肠杆菌作用较差；苯唑西林、氨苄西林、庆大霉素都有一定量的耐药菌株产生，而重组溶葡萄球菌酶对苯唑西林、氨苄西林、庆大霉素耐药菌株同样具有良好抑杀效果。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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Reasons for performing study: Clinical experiences indicate that centesis of the lateral compartment of the femorotibial joint is often unsuccessful. Objective: To determine the accuracy of 2 published and 2 unpublished techniques of centesis of the lateral compartment of the femorotibial joint. Hypothesis: It was hypothesised that a drug can be administered into the lateral compartment of the femorotibial joint via a diverticulum of this joint that surrounds the medial aspect of the long digital extensor tendon and that this technique is more accurate than described techniques of centesis of this compartment. Methods: Twenty‐four stifles of 12 horses were divided equally into 4 groups and a radiocontrast medium injected into the lateral compartment of the femorotibial joint of each group using a hypodermic needle inserted: 1) caudal to the lateral patellar ligament and proximal to the tibial plateau, 2) caudal to the long digital extensor tendon and proximal to the tibial plateau, 3) between the long digital extensor tendon and bone of the extensor groove of the tibia or 4) directly through the long digital extensor tendon until it contacted bone. Twelve veterinary students who had no experience using any of these techniques performed the injections. Accuracy of each technique was determined by examining radiographs obtained after injecting the contrast medium. Results: The most successful technique for arthrocentesis was by inserting a needle through the long digital extensor tendon. This approach was successful in all attempted cases, whilst other techniques had lower rates of success. Conclusions: The lateral compartment of the femorotibial joint can be accessed accurately by inserting a needle through the long digital extensor tendon as it lies within the extensor groove. Other techniques may not be as accurate for clinicians inexperienced in arthrocentesis of the lateral compartment of the femorotibial joint. 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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Marioni-Henry K Vite CH Newton AL Van Winkle TJ 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(6):851-858
A retrospective review of records of 205 cats with histologically confirmed disease of the spinal cord was performed to identify the prevalence of disease in this nonrandomly selected population of cats. Clinical records were reviewed, and age, duration of neurologic illness, and clinical and histopathologic findings in cats with spinal cord disease were abstracted. Disease processes were classified into 7 categories and 23 groups. The most common diseases affecting the spinal cord of cats were feline infectious peritonitis (FIP), lymphosarcoma (LSA), and neoplasia of the vertebral column secondarily affecting the spinal cord. Information on age, onset and duration of clinical signs, and lesion localization at the postmortem examination in cats belonging to the 7 categories of disease were analyzed to create a practical list of differential diagnoses. Cats were also subcategorized into 3 groups based on their age at death. FIP was the most common disease of cats younger than 2 years of age. LSA and vertebral column neoplasia were the most common diseases affecting cats between 2 and 8 years of age. Vertebral column neoplasia was the most common disease affecting cats older than 8 years of age. Results of this histopathologic study showed that FIP and LSA were the most common disease processes affecting the spinal cord of cats. However, at least 21 other groups of diseases and their relative prevalence were identified. 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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根据JJF1059-1999《测量不确定度评定与表示》建立了高效液相色谱法测定鸡肉中土霉素残留测量的不确定度评定方法,评定过程包括建立测量程序、分析不确定度来源、对不确定度分量进行量化。将不确定度归类为两大类。随机效应导致的不确定度可以通过一系列具有统计意义的重复观测值进行A类评定;系统效应导致的不确定度通过各测量器具、设备的说明书、校准证书提供的参数进行B类评定。本方法鸡肉中土霉素残留量的相对合成标准不确定度约为3.9%。 相似文献
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以5个金柑属品种、枳壳、软枝酸橘及广西土柠檬实生苗为材料,进行复水成活率、旱害指数、叶片相对含水量、超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、丙二醛（MDA）、脯氨酸等生理指标及隶属函数的综合分析,为柑橘抗性砧木品种选育提供参考。结果表明：胁迫20d复水后的成活率融安滑皮金柑和长寿金柑最高,为100%,软枝酸橘最低,为88.9%;随着干旱胁迫加剧,各品种的旱害指数呈上升趋势,长寿金柑的旱害指数最低,为0.25,软枝酸橘的旱害指数最高,为0.61;从叶片相对含水量的结果来看,长寿金柑的变化幅度最小,软枝酸橘的变化幅度最大,在第18d时分别为61.19%和37.85%;3种保护酶之间相互协同作用减轻植株受害程度,其活性变化幅度与丙二醛和脯氨酸的含量变化相似。利用隶属函数法综合分析,得出各品种的抗旱性强弱依次为长寿金柑>枳壳>融安滑皮金柑>融安金柑>山金柑>广西土柠檬>金枣>软枝酸橘。 相似文献
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分别用CIDR（孕酮阴道栓）＋PMSG（处理1）和CIDR＋PMSG＋LH（处理2）两种处理方法对山羊进行同期发情处理。结果发现,两种处理均以撤栓后第1d发情率最高。撒栓后0-24h和24h-48h的发情率,处理1和处理2（61．4％,13．6％和48％,20％）之间差异不显著;撤栓后48h-72h的发情率,处理1（4．59,6）显著低于处理2（24％）（P〈0．05）;撤栓后0～72h发情率,两种处理（79．5％,92％）无显著差异（P〉0．05）。从手术时对卵巢的观察可以看出,有大卵泡或排卵的发情羊,处理1为91．2％,处理2为100％,二者无显著差异;排卵的发情羊,处理1为（58．8％）显著低于处理2为（91．2％）（P〈0．05）。 相似文献