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谷氨酰胺对肉仔鸡生产性能及免疫机能的影响 总被引：7，自引：0，他引：7
为了研究日粮中添加谷氨酰胺(Gln)对肉仔鸡生产性能及免疫机能的影响,本试验选取270只1日龄AA肉仔鸡随机分成3组,分别在日粮中添加0、0.2%、0.4%Gln,测定各组生长及屠宰性能、免疫器官指数、新城疫HI抗体滴度和外周血T淋巴细胞百分率.结果表明:日粮添加0.2%、0.4%Gln对肉仔鸡生长、屠宰性能有较好的促进作用,并能加快胸腺和脾脏发育,延缓法氏囊退化;添加Gln可提高新城疫HI滴度,其中以14、28日龄时0.2%的添加剂量提高最为显著(P<0.05);Gln可促进肉仔鸡外周血T淋巴细胞的分裂增殖,其中以42日龄时0.2%的添加剂量提高最为显著(P<0.05).由此可知,日粮中添加0.2%、0.4%Gln能够提高肉仔鸡生产性能、增强免疫功能,其中0.2%的添加量较好. 相似文献
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为研究复方中草药对断奶仔猪细胞免疫、体液免疫和生产性能的影响。选用48头20日龄仔猪,随机分为2组,试验组在23~29日龄、53~59日龄按饲料1%添加中药粉剂,对照组不添加药物,试验组和对照组由同一人员饲养,其饲养条件、免疫程序相同。试验结果显示,复方中草药制剂在断奶初期的添加,对免疫功能和生产性能的改善不明显,在53~59日龄的添加,能提高仔猪的细胞免疫作用和生产性能,85日龄试验组外周血液T淋巴细胞百分比显著高于对照组(P0.05),85~130日龄试验组每头增重极显著高于对照组(P0.01);猪瘟和猪伪狂犬抗体阳性率比较,各阶段差异均不显著。试验表明复方中草药能提高仔猪的细胞免疫作用和生产性能。 相似文献
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80只1日龄Arber Acros肉鸡,随机分为2组,每组4个重复,每重复10只鸡。第1组为对照组,饲喂基础日粮;第2组为试验组,饲喂添加1%中草药添加剂的基础日粮,研究中草药添加剂对肉仔鸡生产性能和免疫功能的影响。结果表明,饲料中添加1%中草药添加剂可显著提高肉仔鸡的饲料转化率、胸腺和脾脏质量、指数、T淋巴细胞数量及新城疫HI抗体滴度,也可提高红细胞C3b受体花环率（C3bRR）及红细胞免疫复合物花环率（ICR）;对肉鸡平均日增质量、法氏囊质量及指数均无明显影响。 相似文献
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通过SPF鸡服用某复方中药后,了解免疫器官指数、外周血液中CD 3、CD 4、CD 8及TCR 1T淋巴细胞百分比含量及IgG的变化,探索某复方中药对禽流感病毒作用的免疫机理.结果表明:给药组免疫器官指数、外周血液中CD 3、CD 4、CD 8及TCR 1 T淋巴细胞百分比含量及IgG均比对照组高.所以,该复方中药可促进SPF雏鸡免疫器官的发育和迅速成熟,提高SPF鸡的细胞免疫及体液免疫功能. 相似文献
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试验旨在研究转植酸酶基因玉米对肉仔鸡血液生理生化指标和免疫功能的影响。采用单因子完全随机设计,将160只1日龄AA肉仔公雏鸡分成2组,分别为对照组和试验组,每组10个重复笼,每个重复笼8只鸡。对照组饲喂非转植酸酶基因亲本玉米日粮,试验组饲喂转植酸酶基因玉米日粮,两组鸡在试验期间饲喂日粮中的可利用磷等所有养分含量均相等或相近。试验期为42 d,分为1~21和22~42日龄2个饲养阶段。结果显示,试验组与对照组相比,42日龄肉仔鸡的血液生理生化指标、免疫器官指数、血清新城疫抗体滴度和全血T淋巴细胞转化率均无显著差异(P＞0.05);21日龄肉仔鸡的血清新城疫抗体滴度也无显著差异(P＞0.05)。因此,转植酸酶基因玉米日粮对肉仔鸡未产生不良影响,可安全饲喂于肉仔鸡。 相似文献
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纯中药饲料添加剂对肉仔鸡免疫功能的影响 总被引：4，自引：0，他引：4
实验用15日龄北京爱拔益加AA肉仔鸡120只。随机分为3组，用新城疫弱毒苗免疫，然后在饲料中添加纯中药饲料添加剂方Ⅰ和方Ⅱ，于不同日龄采血，检测血清中HI抗体水平，结果表明，实验组（即纯中药添加组）HI抗体水平与对照组相比有升高的趋势，作血涂片经α－醋酸萘酯酶染色发现，实验组T淋巴细胞的阳性率显著高于对照组（P＜0．05）。证明纯中药饲料添加剂方Ⅰ和方Ⅱ有增强肉仔鸡体液免疫和细胞免疫的功能。 相似文献
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甲哨唑对鸡细胞免疫和体液免疫功能的作用 总被引：3，自引：0，他引：3
本试验观察了甲硝唑对鸡细胞免疫和体液免疫功能的作用，并与左旋咪唑进行了比较。结果表明2．5－400mg/kg甲哨唑可显著促进鸡外周血T淋巴细胞增殖，但对B淋巴细胞无明显影响；可使CD4^ /CD8^ 淋巴细胞比值明显升高；可使鸡外周血淋巴细胞诱生IL－2活性明显增强；可使鸡体内抗颗粒性胸腺依赖性抗原SRBC抗体滴度、抗可溶性胸腺依赖性抗原BSA抗体滴度和抗非胸腺依赖性抗原BA抗体滴度明显升高；可使鸡血清总补体活性明显升高。与甲硝唑比较，左旋咪唑也存在着非常类似的作用，2．5－10mg/kg左旋咪唑可明显促进外周血T淋巴细胞增殖，可使CD4^ /CD8^ 淋巴细胞比值明显升高；可使鸡外周血淋巴细胞诱生IL－2活性明显增强；可使鸡体内抗SRBC抗体滴度、抗BSA抗体滴度和抗BA抗体滴度均明显升高；10mg/kg左旋咪唑甲硝唑可使血清补体总活性升高。上述结果表明甲硝唑和左旋咪唑两种咪唑类化合物在促进鸡细胞免疫和体液免疫可能存在着相似的作用机制。 相似文献
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为评价兔出血症病毒(RHDV)VP60基因在小鼠体内诱导产生体液免疫和细胞免疫情况,本研究构建了RHDV VP60基因的真核表达质粒pcDNA-VP60,并将其免疫小鼠。利用ELISA方法检测特异性抗体和小鼠外周血细胞因子水平,MTT法和流式细胞术(FACS)检测小鼠外周血T淋巴细胞增殖情况和T淋巴细胞亚群的动态变化。抗体检测结果显示,重组质粒免疫组抗体水平在免疫后5周～6周达到峰值,而且与对照组比较差异显著(p<0.05);细胞因子检测结果显示,重组质粒免疫组血清中IFN-γ、IL-2、IL-4因子水平随免疫时间延长而升高,并且能够维持较高水平,与对照组比较差异显著(p<0.05);T淋巴细胞检测结果显示,重组质粒免疫组T淋巴细胞明显增殖,CD4+T淋巴细胞数量免疫后明显增高,与对照组比较差异显著(p<0.05);所有检测指标显示重组质粒免疫组和灭活苗免疫组比较均无显著差异(p>0.05)。以上结果表明pcDNA-VP60重组质粒可以诱导小鼠产生特异性体液免疫和细胞免疫,为研制预防兔病毒性出血症的候选DNA疫苗提供了实验依据。 相似文献
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N Lacetera U Bernabucci B Ronchi A Nardone 《American journal of veterinary research》2001,62(7):1020-1024
OBJECTIVE: To determine the effects of moderate feed restriction, single or twin pregnancy, and subclinical pregnancy toxemia (PT) on immune responses of ewes. ANIMALS: 16 Sardinian ewes. PROCEDURE: Six weeks before lambing, ewes were assigned to 1 of 2 groups (n = 8/group) matched for number of fetuses, body condition score, and plasma glucose, non-esterified fatty acids (NEFA), and beta hydroxybutyrate (BHBA) concentrations, and feed intake was restricted for 1 of the groups. Cell-mediated immunity was evaluated in vivo and in vitro. Humoral immunity was evaluated in vivo by determining production of IgG antibodies against keyhole limpet hemocyanin (KLH). RESULTS: Four ewes developed subclinical PT (plasma BHBA concentration > 0.86 mmol/L without any clinical signs of disease). Whether feed was restricted and type of pregnancy (single vs twin) did not have any significant effects on cell-mediated and humoral immune responses. Ewes with subclinical PT had significantly lower in vitro proliferation of phytohemagglutinin-stimulated peripheral blood mononuclear cells and significantly lower values for KLH-specific IgG than did healthy ewes. Plasma BHBA and NEFA concentrations were negatively correlated with in vitro proliferation of peripheral blood mononuclear cells; plasma NEFA concentration was negatively correlated with values for KLH-specific IgG. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that subclinical PT may be associated with impairments in cell-mediated and humoral immune responses in sheep. 相似文献
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网状内皮组织增殖病病毒感染SPF雏鸡外周血液T,B淋巴细胞数量变化 总被引：4，自引：1，他引：3
应用ＳＰＡ菌体花环法检测网状内皮组织增殖病病毒（ＲＥＶ）感染ＳＰＦ雏鸡外周血液Ｔ、Ｂ淋巴细胞数量动态变化。结果发现，１日龄ＳＰＦ雏鸡感染ＲＥＶ后７～４９ｄ外周血液Ｔ淋巴细胞数量，１４～４９ｄＢ淋巴细胞数量均明显低于对照组。表明感染ＲＥＶ的ＳＰＦ雏鸡外周血液的细胞免疫和体液免疫水平均下降。 相似文献
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中药复方多糖对雏鸡B淋巴细胞免疫功能的影响 总被引：4，自引：0，他引：4
360只刚出壳的健康雏鸡,随机分为4组,每组90只,从出壳后第5天分别灌喂中药复方多糖、中药复方水煎剂、黄芪多糖、生理盐水,连喂7 d。于12、15、20、25、30、37、44、51日龄,每组随机取10只鸡。通过HA-HI实验、EA玫瑰花环实验,检测雏鸡B淋巴细胞的免疫功能;将鸡宰杀,完整摘取法氏囊,称重,测定法氏囊指数。实验结果表明,灌服中药复方多糖、复方水煎剂以及黄芪多糖的实验组,其抗体效价、EA玫瑰花环形成率、法氏囊指数值均提高,其中中药复方多糖效果较好,复方水煎剂效果优于黄芪多糖;中药复方多糖与复方水煎剂、黄芪多糖差异显著(P<0.05),与生理盐水对照组比较差异极显著(P<0.01)。 相似文献
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ABSTRACT: To investigate immune responses upon re-infection with Lawsonia intracellularis, local and peripheral humoral and cell-mediated immune responses to primary and challenge inoculations were studied in 22 pigs. Pigs were orally inoculated with virulent L. intracellularis at the age of 5-6 weeks, treated with antibiotics and challenged with a re-inoculation (RE) at the age of 12 weeks. Treatment control (TC) pigs received only the primary inoculation and challenge control (CC) pigs received only the secondary inoculation at 12 weeks of age. Following this regimen, all RE pigs were protected against the re-infection as defined by reduced colonisation and pathology of intestinal mucosa, absence of bacterial shedding and without increase in serum acute phase protein response. In the protected RE pigs, serum IgG responses were variable with both high and low responders. Serum IgA responses were not boosted by the re-inoculation, since identical intestinal IgA responses developed in response to the inoculation in both the susceptible CC pigs and the protected RE pigs. A memory recall cell-mediated immune response developed in RE pigs which was significantly stronger compared to the primary response in age-matched CC pigs as assessed by whole blood IFN-γ assay and by calculation of IFN-γ integrated median fluorescence intensity (iMFI) after flow cytometry. The major IFN-γ producing cells were identified as CD8+ and CD4+CD8+ double positive lymphocytes. The results indicate that cell-mediated immune responses are likely mediators of protective immunity against L. intracellularis, with CD8+ effector cells and CD4+CD8+ double positive memory T cells as main contributors to the antigen-specific IFN-γ production. 相似文献
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T cell activity is a critical component of immunity to bovine respiratory syncytial virus (BRSV). We tested the effects of immunization by modified-live and inactivated BRSV vaccines on cell-mediated and humoral immunity in young calves. The two forms of vaccine stimulated similar serum neutralizing antibody production, although the early kinetics of those responses differed. CD4+, CD8+, and gammadelta T cells were analyzed before and after immunization for BRSV-specific in vitro recall responses, as evaluated by CD25 upregulation measured by flow cytometry. Modified-live virus (MLV) primed each of the three subsets for statistically significant in vitro responses to antigen. Inactivated vaccine also primed each T cell population for significant antigen-driven CD25 upregulation, including responses by CD4+ and gammadelta T cells that were stronger and longer-lasting than those primed by MLV. Monoclonal antibody was used in additional assays to block MHC class I during incubation of BRSV antigen with peripheral blood mononuclear cells from an animal in the inactivated vaccine group. The recall response by CD8+ T cells was more inhibited by this treatment than the other subsets, further suggesting that the inactivated vaccine had primed antigen-specific CD8+ T cells. In summary, the data indicate that balanced BRSV-specific T cell responses can be induced by inactivated, as well as modified-live, conventional vaccines, which may implicate an alternative pathway of MHC class I antigen presentation. 相似文献
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Zuckermann FA Garcia EA Luque ID Christopher-Hennings J Doster A Brito M Osorio F 《Veterinary microbiology》2007,123(1-3):69-85
The efficacy of two different types of commercial vaccines against PRRSV (Euro-type) was evaluated based on clinical parameters upon challenge as well as post-challenge virological profiles (viremia and viral load in tissues upon necropsy, measured in both cases by quantitative real time PCR). In an attempt to establish correlates of protective immunity, two commonly proposed parameters predictive of immunity were measured: (1) serologic responses (ELISA and neutralizing antibodies), (2) frequency of gamma interferon-producing cells in peripheral blood mononuclear cell fraction. The vaccines compared consisted of two commercially available products that are regularly marketed in Spain: one modified live virus and one killed vaccine. The efficacy assay was carried out by vaccinating twice 3 weeks apart groups of 5 and-a-half month-old female swine and then challenging them with a European type 1 PRRSV strain (Lelystad). The results obtained indicate that the modified live virus vaccine was the only type of vaccine capable of establishing protective immunity, as measured by viral load in blood and tissues. The killed vaccine, in spite of this product evoking a spontaneous interferon-gamma response and post-challenge titers of virus-neutralizing antibody, evoked no measurable protective immunity. In the case of the modified live vaccine, the protection exhibited did not appear to be based on humoral but rather on cell-mediated immunity. 相似文献