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建立了德拉昔布咀嚼片溶出度测定的高效液相色谱法,并对德拉昔布咀嚼片和Deramaxx咀嚼片的溶出度进行比较。方法为浆法,转速为50 r/min,0.8%十二烷基硫酸钠为溶出介质,于5、10、15、20、30、40、50 min分别采样;采用Waters XSelect H88 C18(4.6 mm×250 mm,5μm)色谱柱,磷酸盐缓冲液(pH4.5)-乙腈(52∶48,V/V)为流动相,检测波长252 nm;柱温30℃;外标法定量。结果表明,德拉昔布在5~25μg/mL浓度范围内线性良好(r2=0.9992),检测限与定量限分别为0.81μg/mL、2.57μg/mL;平均回收率为101.8%±0.91%;德拉昔布咀嚼片与Deramaxx溶出度在30 min内分别为87.78%±1.91%和88.76%±2.05%,符合规定。试验所建立的方法简便、准确、专属性强,可用于德拉昔布咀嚼片的溶出度测定。 相似文献
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为建立测定AC4原料药含量的高效液相色谱(HPLC)测定方法,色谱柱:Diamosil C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.2%磷酸水溶液,65∶35(v/v),流速为1.0 m L/min,检测波长251 nm。结果显示,AC4浓度在20~200μg/m L范围与峰面积呈现良好的线性关系,回归方程A=43743ρ+26 016,R2=0.9999(n=5),平均回收率100.3%,RSD为0.54%,样品至少在8 h内稳定。表明该法快速简捷、准确度高,适用于AC4原料药含量测定。 相似文献
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建立了测定阿德呋啉原料及口服制剂含量的高效液相色谱法。采用Hypersil SI色谱柱(4.6 mm×200 mm,5μm),以甲醇∶异丙醇(25∶75,v/v)为流动相,检测波长260 nm,流速1.0 mL/min,进样量10μL,柱温30℃。结果:阿德呋啉浓度在10～200μg/mL时,其峰面积与浓度的线性关系良好(r2=0.999),无杂质干扰,平均回收率为99.2%,RSD为0.8%。说明本方法操作简单、分析快速、结果准确,适用于阿德呋啉原料及口服制剂的含量测定。 相似文献
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应用反相高效液相色谱法(RP-HPLC)测定复方布他磷注射液中布他磷的含量,采用C8色谱柱(4.6 mm×250 mm,粒度:5μm),以甲醇:0.1%磷酸水溶液(10∶90,V∶V)为流动相,流速1 ml/min,示差折光检测器检测。该反相高效液相色谱法测定的布他磷浓度在0.64~0.96 mg/mL范围内有良好的线性关系;回归系数r2=0.9992;样品溶液至少在24 h内稳定(RSD=0.13%);进样精密度(RSD=0.18%)良好;高、中和低三种浓度下布他磷的回收率均大于99%(RSD<1.0%),回收率良好。本法简便、快速、准确,可用于复方布他磷注射液中布他磷含量的测定。 相似文献
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建立盐酸左旋咪唑片中盐酸左旋咪唑含量测定的高效液相色谱法。采用Agilent Eclipse XDB-C18(250 mm×4.6 mm),5μm色谱柱,Agilent 1260DAD检测器,流动相为0.05 mol/L磷酸二氢钾溶液(三乙胺调节pH值至7.0)-乙腈(80∶20,v/v);进样量为20μL;柱温为30℃;流速为1.0 mL/min;检测波长为214nm。结果表明:盐酸左旋咪唑在1.024～102.398μg/mL范围内线性关系良好(r=1.0000),高、中、低浓度回收率分别为97.4%、99.7%、99.3%。该方法定性、定量准确,适用于盐酸左旋咪唑片中盐酸左旋咪唑的含量测定。 相似文献
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UPLC和HPLC法检测猪肉中磺胺类药物残留的比较 总被引：1，自引：0，他引：1
比较了超高效液相色谱法与高效液相色谱法测定猪肉中磺胺类药物残留的结果。采用农牧发[2001]38号动物源食品中磺胺类药物残留的检测方法有关标准,将原高效液相色谱方法转换并优化为超高效液相色谱方法。高效液相色谱方法色谱柱为Waters Symmetry C18柱(4.6 mm×250 mm,5μm),流动相:乙腈-甲醇-水-乙酸(2∶2∶9∶0.2),流速:1 mL/min,进样量:50μL;超高效液相色谱方法色谱柱为Waters Acquity UPLC BEH RP18柱(2.1 mm×50 mm,1.7μm),流动相:乙腈-甲醇-水-乙酸(2∶2∶10∶0.2),流速:0.4 mL/min,进样量:5μL。结果表明超高效液相色谱方法能够替代高效液相色谱分析方法测定猪肉中磺胺类药物残留,既加快了分析速度,达到了样品分析的高通量,减少有机溶剂的使用,又得到更高的分析灵敏度。 相似文献
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笔者建立了高效液相色谱法测定兽用复方酮康唑乳膏中酮康唑含量。样品经80℃水浴用乙醇提取等前处理,采用Agilent ZORBAX Eclipse(4.6 mm×150 mm,5μm)色谱柱;流动相为甲醇-水（74∶26）;流速为1.0 mL/min;柱温为30℃;检测波长为239 nm。结果酮康唑在0.4015～1.2016 mg/mL范围内呈良好线性,r2=1.000。样品平均回收率为99.52%,RSD为0.70%。该法简便可靠,可测定兽用复方酮康唑乳膏中酮康唑含量。 相似文献
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研究建立了梅花鹿血液中卵磷脂含量测定的高效液相色谱法。对采集的梅花鹿血液,经3000r/min离心获得血清,血清通过0.45μm的微孔滤膜过滤后进样测定。色谱柱为C18柱(150mm×4.6mm),流动相为甲醇∶乙腈(50∶50),流速为2.0mL/min,紫外检测波长为206nm。结果表明:本方法在浓度为0~159.84μg/mL的浓度范围内峰面积与浓度呈线性相关,r=0.9997,变异系数RSD为0.66%,平均回收率为96.45%,该方法经济、简便、快速、灵敏度高、重现性好,适用于梅花鹿血液中卵磷脂的血药浓度检测。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献