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1.
用禽流感免疫胶体金试验诊断一发病率高、死亡快、疑似禽流感或禽I型副粘病毒感染的鹅病例,结果为禽流感阴性;取病鹅肝、脾混合病料进行病毒分离,分离鸡胚尿囊液能凝集鸡红细胞(RBC),HA滴度为6log2,并可被康复鹅血清、抗鸡新城疫(NDV)阳性血清所抑制,而不能被禽流感(H5、H9)标准阳性血清抑制。分离病毒人工感染易感雏鹅,引起100%的发病和死亡,并有与自然病例相似的症状和病变;人工感染42日龄SPF鸡出现100%的发病和死亡。根据临床症状和病变情况、免疫胶体金试验以及病毒分离和鉴定,证明该鹅群患禽I型副粘病毒病。说明用禽流感免疫胶体金试验可以实现对禽流感、禽I型副粘病毒病的快速鉴别诊断。  相似文献   

2.
禽流感是由禽流感病毒引起的禽类传染病,是危害世界养鸡业的主要疫病之一。阐述了鸡高致病性禽流感的发病症状、流行趋势及病原特征,并从综合防控、免疫预防以及加强鸡场饲养管理等方面介绍了鸡高致病性禽流感的防控措施  相似文献   

3.
奇峰  托娅 《内蒙古畜牧科学》2011,(12):96-97,130
禽流感是由禽流感病毒引起的禽类传染病,是危害世界养鸡业的主要疫病之一。阐述了鸡高致病性禽流感的发病症状、流行趋势及病原特征,并从综合防控、免疫预防以及加强鸡场饲养管理等方面介绍了鸡高致病性禽流感的防控措施  相似文献   

4.
为查明贵州省铜仁市某蛋鸡场11月龄蛋鸡发病死亡的原因,采集病死鸡病料进行细菌分离培养,以及禽流感病毒、新城疫病毒、鸡安卡拉病毒、鸡毒支原体的核酸检测。结果:病料中未分离出细菌;鸡安卡拉病毒和鸡毒支原体核酸检测均为阳性,禽流感病毒、新城疫病毒核酸检测为阴性。结论:鸡场病例存在鸡毒支原体与鸡安卡拉病毒混合感染。  相似文献   

5.
近期,笔者所在地区部分鸡场出现批量鸡流行病,显症为"流涕、喘咳、食欲衰退、精神萎靡、脱水及死亡",场主多数向笔者咨询会不会是鸡禽流感?为加深大家对鸡禽流感的认识,笔者简述其发病机理,提出综合防制方法供大家参考。(一)鸡禽流感病概述该病是发生于禽类的一种病毒性传染病,可引起鸡和火鸡的大批量死亡。有"欧洲鸡瘟"的别称。一度引起群众恐慌的鸡禽流感,实际是指高致病性鸡禽流感,目前,我国已经将  相似文献   

6.
乔健 《中国家禽》2004,26(14):41-42
禽流感的传播为粪便、污染器具、病死鸡的接触传播.以及气溶胶(微滴)或暴露于病毒污染物的间接接触传播。尚未发现垂直传播但病鸡产蛋带毒。对鸡胚的致死率高。最初的感染源常见异种禽如鸭,野生禽类特别是迁徙水禽。禽流感传播速度快,有时间隔几个鸡场发病。  相似文献   

7.
禽流感(AI)是由A型流感病毒(AIV)引起的一种禽类病毒性烈性传染病,OIE把该病定为A类烈性传染病。许多家禽和野鸟对A型禽流感病毒都有易感性,发病和危害最大的主要是鸡、火鸡。鸭、鹅感染H5亚型流感病毒死亡率很高,经济损失很大。鸡感染禽流感后可表现出严重的呼吸系统疾病,出现产蛋量下降、致死率很高的急性出血性临床症状;也可表现为轻微的呼吸道感染、轻微产蛋率下降,  相似文献   

8.
正禽流感是危害养鸡业主要疾病之一,该病又称为真性鸡瘟或欧洲鸡瘟,禽流感发病具有明显的季节性,以秋冬季节多发。本文根据禽流感的流行特点,并对禽流感的秋冬季综合防治进行综述,以期为养鸡生产实践中提供指导意义。禽流感是世界养禽业的重要疾病之一,可以感染不同的禽类,当禽类感染了禽流感后,造成了很强的死亡率,给养禽业造成严重的经济损失,严重影响着世界养禽业的发展。鸡的流感病主要由A型流感病毒引起鸡一类  相似文献   

9.
鹅禽Ⅰ型副粘病毒病的快速鉴别诊断   总被引:1,自引:2,他引:1  
用禽流感免疫胶体金试验诊断一发病率高、死亡快、疑似禽流感或禽Ⅰ型副粘病毒感染的鹅病例,结果为禽流感阴性;取病鹅肝、脾混合病料进行病毒分离,分离鸡胚尿囊液能凝集鸡红细胞(RBC),HA滴度为6 log2,并可被康复鹅血清、抗鸡新城疫(NDV)阳性血清所抑制,而不能被禽流感(H5、H9)标准阳性血清抑制。分离病毒人工感染易感雏鹅,引起100%的发病和死亡,并有与自然病例相似的症状和病变;人工感染42日龄SPF鸡出现100%的发病和死亡。根据临床症状和病变情况、免疫胶体金试验以及病毒分离和鉴定,证明该鹅群患禽Ⅰ型副粘病毒病。说明用禽流感免疫胶体金试验可以实现对禽流感、禽Ⅰ型副粘病毒病的快速鉴别诊断。  相似文献   

10.
许多人认为禽流感病毒对雏鸡或生长鸡没有明显的影响,因而往往忽视了对商品肉鸡的防疫。实际上,快大型肉鸡对禽流感病毒十分易感。何况禽流感病毒的血清型较多,不同血清型病毒的毒力差异很大,同一血清型的流感病毒也可能由低毒转化为强毒。我们必须充分认识到对商品肉鸡预防禽流感的重要性,决不可掉以轻心。本文将阐述商品肉鸡禽流感的发生、诊断和防制措施等,为基层养鸡场(户)防制禽流感提供参考。 一、发病情况和临诊症状 该病多发生在 30天龄左右的商品肉鸡,也有提前至14天龄。呈地区性流行,往往相邻的鸡舍相继发病,有“…  相似文献   

11.
12.
采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。  相似文献   

13.
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。  相似文献   

14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。  相似文献   

15.
为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …  相似文献   

16.
以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。  相似文献   

17.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.  相似文献   

18.
用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10~0.82μg/g。表明:蜂蜜应视为天然富硒营养品。  相似文献   

19.
乳酸杆菌益生作用机制的研究进展   总被引:2,自引:0,他引:2  
乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和 Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。  相似文献   

20.
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.  相似文献   

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