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盐胁迫对芨芨草苗期质膜H~+-ATP酶活性及5′-核苷酸酶活性的影响 总被引：1，自引：0，他引：1
以芨芨草(Achnatherum splendens)为试验材料,在不同浓度NaCl和Na2SO4胁迫下,对芨芨草叶片与根系的质膜H+-ATPase和5′-AMPase的活性进行测定。结果表明:在NaCl和Na2SO4胁迫下,芨芨草根系的质膜H+-ATPase活性呈"先升后降"的趋势,当浓度小于300mmol/L时,根系的质膜H+-ATPase和5′-AMPase活性均高于对照。同一盐胁迫下,芨芨草根系中质膜H+-ATPase活性和5′-核苷酸酶活性均显著高于叶片。在Na2SO4胁迫下,芨芨草根系和叶片的质膜H+-ATPase和5′-AMPase活性分别显著高于相同浓度的NaCl胁迫组,芨芨草在Na2SO4胁迫下有更强的抗性。 相似文献
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以芨芨草（Achnatherum splendens）为试验材料,在不同浓度NaCl和Na2SO4胁迫下,对芨芨草叶片与根系的质膜H＋-ATPase和5′-AMPase的活性进行测定。结果表明：在NaCl和Na2SO4胁迫下,芨芨草根系的质膜H＋-ATPase活性呈＂先升后降＂的趋势,当浓度小于300mmol/L时,根系的质膜H＋-ATPase和5′-AMPase活性均高于对照。同一盐胁迫下,芨芨草根系中质膜H＋-ATPase活性和5′-核苷酸酶活性均显著高于叶片。在Na2SO4胁迫下,芨芨草根系和叶片的质膜H＋-ATPase和5′-AMPase活性分别显著高于相同浓度的NaCl胁迫组,芨芨草在Na2SO4胁迫下有更强的抗性。 相似文献
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盐胁迫是影响植物生长、发育以至产量的重要因素,试验通过对碱地风毛菊苗期分别以NaCl和Na2CO3进行胁迫,Na+浓度为0(CK),60,120,180,240,300,360 mmol/L,测定其丙二醛、PM-ATPase活性及5'-AMPase活性,以探讨盐胁迫对其膜系统的影响及其苗期耐盐性.结果表明,盐胁迫下,碱地风毛菊丙二醛含量增大,Na2CO胁迫下,碱地风毛菊根系和叶片中MDA含量在Na+浓度为360 mmol/L时达到最大值,分别为对照的2.15和1.94倍;在NaCl胁迫下,碱地风毛菊叶片中MDA含量最大为对照的1.22倍.同一盐胁迫下,碱地风毛菊根系中PM-ATPase活性和5’-AMPase活性显著高于叶片(P＜0.05),碱地风毛菊根系中PM-ATPase活性最大为56.29μg磷酸/(mg蛋白·h),而5'-AMPase活性最大为53.91 μg磷酸/(mg蛋白·h).2种盐胁迫下PM-ATPase和5’-AMPase活性差异显著.总之,盐胁迫下,碱地风毛菊根系比叶片受到的损害小,碱地风毛菊更耐NaCl胁迫. 相似文献
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针对西北盐碱地区栽培土壤资源有限问题,研究混合盐碱胁迫对醉马草种子萌发及幼苗生理相关酶特性的影响,旨在探讨与揭示醉马草对盐碱土壤环境的适应机制,为西北盐碱地区的耐盐草种的选育及栽培提供理论基础.为模拟西北地区复杂的盐碱环境,本研究将中性盐(NaCl、Na2SO4)和碱性盐(NaHCO3、Na2CO3)按不同比例混合(A:NaCl:Na2SO4=1:1;B:NaCl:Na2SO4:NaHCO3=1:2:1;C:NaCl:Na2SO4:NaHCO3:Na2CO3=1:9:9:1;D:NaCl:Na2SO4:NaHCO3:Na2CO3=1:1:1:1;E:NaCl:Na2SO4:NaHCO3:Na2CO3=9:1:1:9),并设置50、100、150、200 mmol·L-14个盐浓度梯度对带菌(E+)和不带菌(E-)醉马草种子萌发和幼苗生长阶段进行胁迫,分析盐胁迫下醉马草种子萌发阶段的发芽率、发芽势、发芽指数、活力指数、平均发芽时间及幼苗生长阶段的苗长、胚根长度、根冠比、抗氧化酶(POD、SOD、CAT)活性、丙二醛(MDA)和脯氨酸(proline)含量.并通过隶属函数和主成分分析对不同盐胁迫下的醉马草耐盐性进行综合评价.结果表明:5种盐碱胁迫均使醉马草发芽率、发芽势、发芽指数、活力指数降低及平均发芽时间延长,且随盐浓度的增加,种子萌发受到显著抑制(P<0.05);盐碱胁迫亦随盐浓度的增加使醉马草胚根长度、根冠比显著降低.除C、E处理外,其余混合盐碱胁迫下的醉马草幼苗生理指标(POD、SOD、MDA、proline)均随盐浓度的上升而下降.隶属函数和主成分分析显示不同混合盐碱胁迫下的醉马草耐盐性大小顺序为A>D>B>C>E.综上可知,盐碱胁迫显著抑制醉马草种子萌发和幼苗生长(P<0.05),其中碱性盐(NaHCO3、Na2CO3),尤以Na2CO3对醉马草种子萌发和幼苗生长抑制作用强于中性盐.活力指数、胚根长度、POD、SOD、proline为评价醉马草耐盐性的主要指标.E+醉马草耐盐性优于E-,内生真菌可有效提高醉马草渗透调节和活性氧清除能力,降低膜脂过氧化作用,提高醉马草耐盐性. 相似文献
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采用不同浓度的NaCl、Na2SO4、NaHCO3和Na2CO3四种盐的混合液及NaCl、NaHCO3两种单盐分别处理大花飞燕草（Delphinium grandiflorum）种子,观察并测定不同浓度盐碱胁迫下种子的发芽率、发芽指数、幼苗生物量等,根据测定的数值计算出种子活力的指标。结果表明,低浓度NaCl盐溶液有利于大花飞燕草生物量的积累,重度的混合盐溶液胁迫下,不论浓度高低都不利于其萌发、生长;高浓度盐碱溶液胁迫对其活力影响很大,盐浓度达150 mmol·L-1时,不同处理下的种子活力都为0。盐胁迫和碱胁迫同时作用于大花飞燕草时,两者互作共同影响种子活力。 相似文献
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采用不同浓度NaCl、Na2SO4、NaHCO3、Na2CO3单盐溶液对长穗偃麦草种子进行胁迫处理,测定种子发芽率、发芽势、发芽指数、活力指数以及幼苗芽长和根长等指标,研究长穗偃麦草种子在不同盐胁迫条件下的萌发特性。结果表明,与用蒸馏水培养的对照相比,随着NaCl、Na2SO4、NaHCO3、Na2CO3单盐浓度的增加,长穗偃麦草种子发芽率、发芽势、发芽指数、活力指数均呈下降趋势。NaCl、Na2SO4、NaHCO3、Na2CO3对胚芽长和胚根长的抑制规律一致,表现为随盐浓度升高抑制作用增强,且胚根比胚芽对盐浓度反应更敏感;但是低浓度NaHCO3对胚根生长有一定的促进作用。4种单盐对长穗偃麦草种子萌发的抑制作用总体表现为:二价盐>一价盐。 相似文献
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以Na2CO3和NaHCO3对苗期碱地风毛菊(Saussurea runcinata)进行胁迫,探讨其苗期耐碱性及脯氨酸代谢途径.结果表明:NaHCO3和Na2CO3胁迫下,碱地风毛菊中脯氨酸含量增加,最大值为对照的15.5倍;吡咯啉-5-羧酸合成酶(P5CS)活性受到抑制;鸟氨酸δ-氨基-转移酶(δ-OAT)活性升高,最大为12.58 U/μg Protein;碱地风毛菊中脯氨酸合成途径以鸟氨酸(Orn)途径为主;NaHCO3胁迫下脯氨酸降解代谢旺盛,Na2CO3胁迫下,碱地风毛菊叶片中脯氨酸脱氢酶(ProDH)活性下降,根系中ProDH活性增大,脯氨酸降解代谢旺盛. 相似文献
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羊草对不同盐碱胁迫的生理响应 总被引：14，自引：9，他引：14
用不同浓度的NaCl(0~700 mmol/L)和Na2CO3(0~200 mmol/L)对羊草进行胁迫处理,通过测定与生长相关的生理指标,从而分析了2种盐对羊草生长的胁迫作用特点。实验结果表明,羊草对NaCl、Na2CO3处理的最高耐受浓度分别是600和175 mmol/L。NaCl、Na2CO3处理不同程度地影响了羊草的营养生长以及物质代谢,具体表现为分蘖率、平均根茎数、相对含水量、相对生长率、叶绿素、氮含量的降低,其中以Na2CO3胁迫处理组尤为明显。NaCl、Na2CO3破坏羊草质膜功能,K 、电解质外渗率随着盐浓度的增加而增加,这一结果表明质膜的透性增大。TTC(红四氮唑)含量水平反映了NaCl、Na2CO3处理对羊草根系活力的影响,2种盐的处理降低了根系活力,特别是在Na2CO3处理组中,当Na 浓度超过200 mmol/L时,植株根系TTC含量几乎为0。以上各生理指标表明:NaCl、Na2CO3不同程度地影响了羊草生长、光合作用以及物质代谢,其中以Na2CO3处理更为严重。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献