首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 62 毫秒
1.
目标：建立离子色谱-抑制电导检测法测定化学对照品中酒石酸盐的含量。方法：以酒石酸沃尼妙林对照品为例,采用DIONEX IonPac AS19(250 mm×4 mm)阴离子交换色谱柱和Ion Pac AS19保护柱(50 mm×4.0 mm),以22 mmol.L-1氢氧化钾溶液为流动相,流速为1.0 mL.min-1等度洗脱,抑制电导检测器检测,测定酒石酸盐中酒石酸的含量。结果：该方法在1.0~50 μg.mL-1浓度范围内线性关系良好(r=0.999),定量限为0.5μg.mL-1,酒石酸沃尼妙林在3个水平的加样回收率分别为98.79%、98.91%与96.50%,平均回收率为98.07% (RSD=2.20%,n=9)。用此方法测定3个厂家共5批样品,酒石酸沃尼妙林中酒石酸含量的数值与理论值基本一致。结论：本文建立的离子色谱法准确、灵敏,可用于酒石酸盐成盐比例与含量的测定。  相似文献   

2.
建立了毛细管气相色谱法测定巴胺磷溶液含量的方法。试验采用6%氰丙基苯基-94%二甲基硅氧烷为固定相的毛细管柱,柱温240 ℃,进样口温度250 ℃,FID检测器温度250 ℃,载气为氮气,流速3 mL/min,进样量1 μL,分流比15 : 1,内标物质为邻苯二甲酸二丁酯。在该色谱条件下,巴胺磷峰与内标物质峰分离度良好,方法平均回收率为103.5%(n=9,RSD=1.7%),重复性RSD为0.6%,定量限为2.5 μg/mL,巴胺磷在0.025~0.5 mg/mL浓度范围内呈良好的线性关系(r2=0.9999),不同色谱柱、柱温、流速、进样口温度、检测器温度耐用性良好。结果表明,建立的方法专属性好、快速、准确,可用于测定巴胺磷溶液的含量。  相似文献   

3.
建立了阿维菌素粉中非法添加非泼罗尼的液相色谱－串联质谱检测方法,使用C18(100 mm×2.1 mm ,1.7 μm)色谱柱,以水-乙腈为流动相进行梯度洗脱,采用电喷雾离子源负离子检测模式(ESI-)和多反应监测(MRM)模式测定,外标法定量。结果表明,非泼罗尼进样浓度在0.025～20 ng/mL范围内与峰面积呈良好的线性关系(r2＝0.9995);平均回收率为100.2%(n＝15),RSD为7.7%。本方法简便、准确、快速、灵敏,适用于阿维菌素粉中非法添加非泼罗尼的检测。  相似文献   

4.
建立了反向高效液相色谱法(RP-HPLC)测定注射液中甲硫酸新斯的明的含量。采用C18柱(4.6×150 mm,5 μm),以0.1%磷酸水溶液(用三乙胺调pH至3.0)-甲醇(90：10)为流动相,260 nm为检测波长。实验结果表明,甲硫酸新斯的明在10～2000 ng.mL-1范围内线性关系良好,R2=0.9999,平均回收率范围为99.2%～100.8％,RSD=0.52%。该方法简便、精确、重现性好,可用于甲硫酸新斯的明注射液的质量控制。  相似文献   

5.
目的：建立HPLC双波长法同时测定四味穿心莲散中穿心莲内酯和脱水穿心莲内酯的含量。方法：采用XBridge C18色谱柱(4.6 mm×150 mm,5 μm),以甲醇-水(52:48)为流动相,流速1.0ml.min-1;柱温25℃;进样量10μL,检测波长分别为225nm(穿心莲内酯)和254nm(脱水穿心莲内酯)。结果：穿心莲内酯浓度在12.7375μg.mL-1～509.5μg.mL-1范围内,线性良好(r2=0.9996);脱水穿心莲内酯浓度在3.4μg.mL-1～136μg.mL-1范围内,线性关系良好(r2=0.9997)。穿心莲内酯加样回收率平均为98.8%,RSD 为1.0%;脱水穿心莲内酯回收率平均为99.9%,RSD 为0.8%。结论：该方法快捷、灵敏、准确、可靠,可用于四味穿心莲散中穿心莲内酯和脱水穿心莲内酯2个有效成分的同步测定,为四味穿心莲散的质量控制和评价提供科学依据。  相似文献   

6.
建立QuEChERS结合高效液相色谱-串联质谱测定乳粉中黄曲霉毒素B1(aflatoxin B1,AFB1)和黄曲霉毒素M1(aflatoxin M1,AFM1)的检测方法。方法 乳粉用水复溶,乙腈提取,十八烷基键合硅胶和无水硫酸镁净化,在InertSustain-C18柱(2.1 mm×150 mm,3μm)上分离,乙酸铵溶液-甲醇为流动相,梯度洗脱,电喷雾正离子模式扫描,多反应监测,基质添加外标曲线定量。结果 乳粉中AFB1和AFM在0.08~20 μg/kg浓度范围内线性关系良好,r＞0.999。检出限为AFM1 0.02 μg/kg,AFB1 0.03 μg/kg,定量限均为0.05 μg/kg。在定量限的1、5和10倍添加浓度下,平均回收率为91.5%~100.7%。结论 本方法便捷高效、重现性好、结果准确,可以为日常乳粉中黄曲霉毒素检测提供技术参考。  相似文献   

7.
建立了伊维菌素纳米乳中伊维菌素含量的高效液相色谱检测方法(HPLC)。采用C18色谱柱(4.6 × 250 mm，5 μm)，以甲醇-乙腈-水(40∶55∶5)为流动相，流速1.0 mL/min，检测波长254 nm，进样量10 μL，柱温25 ℃。伊维菌素浓度在4.6～92.0 μg/mL时，其峰面积与浓度线性关系良好(r2=0.9996)，回收率高，平均回收率为98.25%~98.76%。精密度良好，RSD均小于2%，色谱峰图无杂质干扰，稳定性高，本操作方法简便，分析快速，结果准确，适用于伊维菌素纳米乳剂的含量测定。  相似文献   

8.
满晨  干小英 《中国饲料》2008,(10):36-37
采用高效液相色谱法,以C18为色谱柱,以甲醇∶水(25∶75)为流动相,检测波长为250 nm,测定葛根中葛根素的含量.结果表明,葛根中葛根素的含量在20～140 μg/mL,线性关系良好(r=0.9999).平均回收率97.40%,RSD=3.0%.本方法测定简便、快速、测定结果准确.  相似文献   

9.
建立高效液相色谱(HPLC)法测定二硝托胺的含量.色谱柱为Agilent ZORBAX Eclipse Plus C18柱(150 mm×1.6 mm,5 μm),流动相为甲醇-水(40∶60),流速1.0 mL/min,柱温25 ℃,检测波长240 nm.二硝托胺对照品浓度在20 μg/mL～100 μg/mL范围内与峰面积呈线性关系,线性回归方程A＝31.20C-9.79,r＝0.999 9(n＝5);平均回收率为99.71%,RSD＝0.32%(n＝5).HPLC法快速、简便,重现性好,准确度高,可用于二硝托胺原料的含量测定.  相似文献   

10.
建立牛肉中加替沙星残留量检测的液相色谱-串联质谱(liquid chromatography-tandem mass spectrometry, LC-MS/MS)方法。牛肉样品经80%(V/V)乙腈溶液提取, Prime HLB固相萃取柱净化。采用0.1%甲酸(V/V)-甲醇溶液(A)和0.1%甲酸(V/V)-水溶液(B)进行梯度洗脱, 电喷雾离子源正离子扫描模式(ESI+),多反应监测(MRM),内标法定量。结果表明,加替沙星进样浓度在1～50 ng/mL范围内与峰面积呈良好的线性关系(r2＝0.999);方法检出限和定量限分别为1.0和2.0 μg/kg;在2.0、4.0、20 μg/kg 3个添加水平上,回收率在64.2%～113.4%之间,相对标准偏差为5.0%～9.7%(n=6)。本方法快速、准确、灵敏,适用于牛肉中加替沙星残留量检测。  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

14.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

15.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

16.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

17.
The purpose of this study was to determine the frequency of different tumor types within a large cohort of cats with intracranial neoplasia and to attempt to correlate signalment, tumor size and location, and survival time for each tumor. Medical records of 160 cats with confirmed intracranial neoplasia evaluated between 1985 and 2001 were reviewed. Parameters evaluated included age, sex, breed, FeLV/FIV status, clinical signs, duration of signs, number of tumors, tumor location(s), imaging results, treatment, survival times, and histopathologic diagnosis. Most of the cats were older (11.3 +/- 3.8 years). Primary tumors accounted for 70.6% of cases. Metastasis and direct extension of secondary tumors accounted for only 5.6 and 3.8% of cases, respectively. Twelve cats (7.5%) had 2 or more discrete tumors of the same type, whereas 16 cats (10.0%) had 2 different types of intracranial tumors. The most common tumor types were meningioma (n = 93, 58.1%), lymphoma (n = 23, 14.4%), pituitary tumors (n = 14, 8.8%), and gliomas (n = 12, 7.5%). The most common neurological signs were altered consciousness (n = 42, 26.2%), circling (n = 36, 22.5%), and seizures (n = 36, 22.5%). Cats without specific neurological signs were common (n = 34, 21.2%). The tumor was considered an incidental finding in 30 (18.8%) cats. In addition to expected relationships (eg, meninges and meningioma, pituitary and pituitary tumors), we found that lesion location was predictive of tumor type with diffuse cerebral or brainstem involvement predictive of lymphoma and third ventricle involvement predictive of meningioma.  相似文献   

18.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

19.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

20.
Comparison of different tests to diagnose feline infectious peritonitis   总被引：1，自引：0，他引：1  
  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号