共查询到20条相似文献，搜索用时 62 毫秒
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为分析不确定度的来源并对其量化,从而提高检测结果的准确性,对酶联免疫吸附(ELISA)法检测家禽活疫苗中禽白血病病毒污染的测量不确定度进行评估。用禽白血病病毒ELISA检测试剂盒,对4批家禽活疫苗进行禽白血病病毒检测,依据《兽医检测实验室ELISA试验测量不确定度评估指南》(CNAS-GL043),分析试验的不确定度来源,并评定各分量的标准不确定度、合成标准不确定度和扩展不确定度。结果显示,每批家禽活疫苗连续3代细胞培养物的禽白血病病毒OD_(650nm)值不确定度范围的上限值均小于0.300,判定为阴性,即所检测4批活疫苗中没有禽白血病病毒污染。ELISA法检测中引入不确定度概念,不仅可以提高检测结果的准确性和可靠性,而且还可以分析不确定度各分量对测量结果的相对贡献,找出影响检测质量的主要因素,从而有利于实验室质量控制和检测质量的提高。 相似文献
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《黑龙江畜牧兽医》2016,(13)
为了分析不同样本对应用ELISA法检测禽白血病阳性检出率的影响,试验将20只10日龄SPF鸡随机均分为试验组和对照组,试验组接种禽白血病病毒,对照组接种生理盐水,7 d后采集各组SPF鸡的肝脏组织、血清、泄殖腔拭子和骨髓进行ELISA检测,统计禽白血病的阳性检出率。结果表明:试验组SPF鸡的肝脏组织、血清和骨髓的禽白血病检测结果相对一致,阳性检出率为100%;而泄殖腔拭子的阳性检出率为70%,相对其他样本结果偏低。说明以泄殖腔拭子为样本时,其ELISA检测结果可能具有假阴性现象,综合考虑,以血清为样本进行禽白血病ELISA检测的阳性检出率更为可靠。 相似文献
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《中国家禽》2016,(4)
本研究对SPF鸡检查法在检测弱毒疫苗中低剂量禽白血病病毒(ALV)污染时的核酸斑点杂交法和ELISA进行了比较。分别以10 TCID_(50)/羽份和5 TCID50/羽份ALV-A人为污染一批商品化新城疫(ND)活疫苗,将污染ND疫苗各接种10只SPF鸡,以ELISA定期检测ALV抗体并利用PCR结合核酸斑点杂交法检测血液中ALV核酸。结果显示,在免疫污染疫苗后连续3周内ALV抗体全部为阴性,而核酸斑点杂交法检测表明自免疫后两周开始就出现ALV阳性。结果提示在利用经典的SPF鸡检查法检测弱毒疫苗中ALV污染时,结合对病原核酸的检测有助于节省检测时间和降低漏检率。 相似文献
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ELISA法检测种蛋卵黄中禽网状内皮组织增生症病毒抗体的研究 总被引：1，自引：1，他引：0
为研究种蛋卵黄中禽网状内皮组织增生症病毒(REV)抗体的检测方法,运用IDEXX公司的REV抗体ELISA检测试剂盒,比较了不同提取方法、不同稀释倍数、不同洗液对卵黄中REV抗体检测结果的影响。结果显示:当用直接提取的卵黄液做300倍稀释检测,用含0.05%吐温-20的蒸馏水洗涤时,卵黄抗体的S/P比值与相应鸡群的血清抗体的S/P比值吻合度最高。应用本方法分别对50枚SPF种蛋和50枚普通鸡蛋进行检测,SPF种蛋均为REV抗体阴性;普通鸡蛋有4枚为REV抗体阳性,阳性率为8%。实验表明,种蛋卵黄ELISA抗体检测法可以代替传统血清抗体检测,为SPF鸡群的REV感染监测奠定了基础。 相似文献
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采用28日龄SPF鸡,对禽脑脊髓炎油乳剂灭活疫苗及弱毒活疫苗进行了免疫效果评估。抗体检测结果表明,疫苗免疫后21日,油乳剂灭活疫苗免疫组抗体水平及抗体转阳率优于弱毒活疫苗免疫组。攻毒保护试验结果表明,禽脑脊髓炎油乳剂灭活苗及弱毒活疫苗均具有良好的免疫保护性,对禽脑脊髓炎病毒强毒VR株的保护率分别为100%、90%。综上所述,灭活疫苗和活疫苗均可有效预防禽脑脊髓炎。 相似文献
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以鸽抗鸡劳氏肉瘤病毒(RSV)血清IgG作为包被抗体及酶标抗体的双抗体夹心法Dot-ELISA,检测禽白血病病毒抗原,其特异性及敏感性与澳大利亚ELISA(用兔抗AMV-P_(27)血清IgG作为包被抗体及酶标抗体)相同。可用以检测卵清中禽白血病病毒抗原及用鸡胚或鸡胚细胞生产的各种疫苗中污染的禽白血病病毒抗原。 相似文献
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为研究以卵黄抗体替代血清抗体判定SPF鸡群J亚群禽白血病病毒(ALV-J)感染状态的可行性,人工接种ALV-J的SPF鸡23周龄时,分别从25只抗体阳性鸡及22只抗体阴性鸡采集血清和卵黄,比较不同稀释度的卵黄与血清中ALV-J抗体的阴阳性吻合率及ELISA检测S/P值相关系数。结果表明,相对于血清抗体,将卵黄1∶400稀释,假阳性和假阴性最少,确定1∶400为卵黄最适稀释度。对40只攻毒SPF鸡和36只同批次单独饲养的空白SPF鸡在25~34周龄,每隔3周采集一次血清,每周采集一次种蛋,共采集304份血清样品和760份卵黄样品。血清按1∶500稀释,卵黄按1∶400稀释,所有血清和卵黄抗体用美国IDEXX公司ALV-J抗体ELISA检测试剂盒检测,同一只鸡同一时段采集的血清和卵黄样品严格在同一次ELISA中检测。结果显示,在25~34周龄,卵黄抗体检测判定结果与血清整体吻合率为82.5%~95%。上述结果表明用卵黄抗体替代血清样品检测来监控SPF鸡群对ALV-J的感染状态是可行的,疫苗生产企业可通过抽检SPF鸡场提供SPF种蛋的卵黄抗体水平来判断SPF鸡场的洁净度。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献