共查询到20条相似文献，搜索用时 63 毫秒
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[目的] 建立微波水解—氨基酸自动分析仪快速测定饲料中色氨酸含量的方法。[方法] 以奶牛全混合日粮饲料（TMR）为研究对象,通过单因素试验和正交试验筛选微波碱水解最佳条件,联合氨基酸自动分析仪LCAK06/Na（4.6 mm×150 mm）色谱柱分离及快速测定饲料中色氨酸含量。[结果] 水解温度是影响饲料色氨酸含量的最主要因素。微波碱水解测定饲料中色氨酸含量的最佳条件是：水解温度170 ℃、水解时间30 min、碱浓度5 mol/L,通过氨基酸自动分析仪测定TMR饲料色氨酸含量为0.14%。色氨酸在0.025~0.600 μmol/mL峰面积与浓度线性关系良好,相关系数（r）为0.999 7。定量限（LOQ）为0.05%,色氨酸回收率93%~104%,精密度RSD为3.58%。[结论] 该方法高效、准确,检测结果与国家标准《饲料中色氨酸的测定》（GB/T 15400—2018）中的常规碱水解法对比无显著差异,对于饲料中色氨酸含量测定具有参考价值。 相似文献
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砷和镉广泛存在于自然界中,为有毒有害元素,动物摄入过量砷和镉时对神经系统、造血系统、骨骼造成损害,而饲料是动物摄入砷和镉的主要来源.所以,对饲料中砷和镉含量制定限量标准已成为饲料卫生必须检测的项目之一.饲料中砷含量测定的现行国家标准是GB/T13079-2006[1].饲料中镉含量测定的现行国家标准是GB/T13082-1991[2]. 相似文献
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《上海畜牧兽医通讯》2017,(3)
正测定饲料中钙含量的国家标准方法是高锰酸钾法《GB/T 6436-2002饲料中钙的测定》,该方法适用于饲料原料和饲料产品中钙含量的测定,其测定原理是将饲料中有机物破坏,钙变成溶于水的Ca~(2+)离子,用草酸铵定量沉淀,用高锰酸钾标准溶液间接滴定钙含量。 相似文献
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《饲料工业》2001,(3)
针对饲料行业，国家质量技术监督局和饲料行业行政主管部门发布的行业标准共 207项，其中国家标准 70项，行业标准 137项，国家标准中有 9项标准是强制性标准，有 47项是检测方法标准，卫生指标检验方法只有 16项。目前这些标准已远远不能满足飞速发展的饲料工业的需要。最近国家又制定、修订了一批新标准，现已发布，它们是： —— GB/T17811— 1999动物蛋白质饲料消化率的测定胃蛋白酶法 —— GB/T17812— 1999饲料中维生素 E的测定高效液相色谱法 —— GB/T17813— 1999复合预混料中烟酸、叶酸的测定高效液相色谱法 —— GB/T178… 相似文献
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无 《畜牧兽医科学(电子版)》2019,(6):58-58
2019年3月22日,中国饲料工业协会与全国饲料工业标准化技术委员会在武汉市举办2019年饲料工业标准宣贯培训班。王黎文秘书长重点对现行《饲料卫生标准》和《饲料标签》两项标准进行了解读,四川省饲料工作总站李云站长、华中农业大学齐德生教授,浙江省兽药饲料监察所朱聪英研究员等9位标准起草专家,分别就《饲料中铅的测定原子吸收光谱法》(GB/T13080-2018)、《饲料中亚硝酸盐的测定比色法》(GB/T13085-2018)和《饲料中黄曲霉毒素B1的测定高效液相色谱法》(GB/T36858-2018)等13项近两年新发布的与《饲料卫生标准》配套的检测方法标准,以及饲料中药物、违禁物质的检测方法标准进行了解读。 相似文献
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中华人民共和国国家标准《饲料检测结果判定的允许误差》(GB/T 1 882 3- 2 0 0 2 )由中华人民共和国国家质量监督检验检疫总局于 2 0 0 2年 9月 6日发布 ,2 0 0 3年 3月 1日实施。为了准确理解和实施该标准 ,现将有关问题阐述如下。在本文以下论述中 ,将《饲料检测结果判定的允许误差》国家标准简称为《标准》。1 《标准》制定与实施的重要意义近些年来 ,我国各级饲料质量监督检验机构经常性地抽样检测饲料产品质量 ,实行奖优惩劣 ,为保证我国饲料产品质量的不断提高起到了积极的促进作用。我国的饲料质量标准和世界各国的饲料质量标准一… 相似文献
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钙和磷是畜禽饲料中的两项重要指标,畜禽对钙和磷的摄入量不足或过量都将严重影响畜禽健康,因此饲料必须经常检测。我们知道,在国家标准方法中,测定饲料中的钙和总磷在采用干法分解样品时,只是盐酸的浓度不同,其余试剂操作过程完全相同。在国标GB/T6436-2002中测钙要求采用1:3盐酸溶液,而在国标GB/T6437-2002中测磷要求采用1:1盐酸溶液,这样测定饲料中钙和总磷 相似文献
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肉牛养殖场饲料中的大肠菌群及沙门氏菌的检测与分析 总被引：1，自引：0，他引：1
[目的]为了了解新疆伊犁地区和阿克苏地区肉牛养殖场的饲料受致病菌污染程度,对所采集的18份饲料样品中的大肠菌群及沙门氏菌进行检测。[方法]沙门氏菌的检测按照中华人民共和国国家标准GB/T13091-2002(饲料中沙门氏菌的检测方法)中所规定的方法进行,大肠菌群的检测按照中华人民共和国国家标准GB/T18869-2002(饲料中大肠菌群的测定)中所规定的方法进行。[结果]麦秆饲料中大肠菌群最高,达到11000个/100g;经加工的饲料(颗粒料、棉籽粕、菜籽粕)中大肠菌群含量都较低,数量低于40个/100g;在所有饲料样品中均未检出沙门氏菌。[结论]所检测的18份肉牛饲料样品均不同程度受到大肠菌群的污染。 相似文献
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本研究选用常见的16种饲料样品作为实验样本,采用国家标准方法《GB/T 6438-2007/ISO 5984:2002饲料中粗灰分的测定》,通过比较试验,分别采用快慢两种降温方法对样品进行灰分测定。结果表明,两种降温方法对灰分测定结果无明显影响,检测结果无明显差异,因此,实验室可以采用快速降温法对饲料进行灰分测定。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献