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《中国动物传染病学报》2019,(1)
为研究抗球虫新药沙咪珠利与鸡血浆中蛋白结合情况,采用平衡透析法模拟沙咪珠利与鸡血浆蛋白结合过程,高效液相色谱法测定透析内液及外液中药物浓度,计算沙咪珠利血浆蛋白结合率。结果表明,鸡血浆中其他成分对测定无干扰,各待测物在选择浓度范围内线性关系均良好,透析外液线性范围为0.1～10μg/mL,透析内液的线性范围为0.5～100μg/mL,提取回收率在94.35%～111.8%之间,日内、日间变异均小于10%,精密度及稳定性结果均符合方法学要求。在设定的3个药物浓度下,沙咪珠利在鸡血浆中的蛋白结合率为92.17%～97.72%,属高血浆蛋白结合率药物,且具有一定的浓度依赖性。 相似文献
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《中国动物保健》2017,(5)
健康杜长大猪16头(体重为18±1kg)随机分为A、B两组,以20mg/kg剂量单次灌服两种氟苯尼考制剂(20%氟苯尼考粉和2%氟苯尼考预混剂),并于给药后不同时间点从前腔静脉采血,采用已建立的高效液相色谱法(HPLC)进行血药浓度测定,利用Win Nonlin软件的非房室模型拟合其血药浓度-时间数据。方法学研究表明本实验建立的HPLC方法专属性良好,在目标峰附近无杂质干扰,线性良好,线性范围为0.03~10μg/mL,相关系数为0.9994。20%氟苯尼考粉主要药动学参数为:Tmax=1.13±0.64h,Cmax=9.61±2.65μg/mL,T1/2=3.84±1.56h,MRT=4.65±0.70h,AUC=52.56±16.99 h*μg/mL;2%氟苯尼考预混剂主要药动学参数为:Tmax=1.31±0.37h,Cmax=8.30±2.17μg/mL,T1/2=3.39±0.35h,MRT=4.93±0.74h,AUC=49.84±17.54 h*μg/mL。比较药动学结果表明20%氟苯尼考粉相对于2%氟苯尼考预混剂的相对生物利用度为105.45%。经T检验和方差分析,二者主要药动学参数无显著差异,在猪体内可视为有相似的药动学过程,但前者在饮水灌服给药时能在更短的时间吸收分布、达到血药峰浓度,且血药峰浓度略高于2%氟苯尼考预混剂,故20%氟苯尼考粉起效更快、效果稍好于2%氟苯尼考预混剂。 相似文献
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为了探索新疆南疆地区表皮葡萄球菌对氟苯尼考注射液的敏感性，试验采用微量肉汤稀释法测定了氟苯尼考注射液对表皮葡萄球菌ATCC 35984和临床分离株的最低抑菌浓度(MIC)、最低杀菌浓度(MBC),对表皮葡萄球菌生长、生物被膜形成的抑制作用及表面疏水性的影响，测定了氟苯尼考注射液的体外抑菌效果及其对表皮葡萄球菌细胞膜完整性的影响。结果表明：氟苯尼考注射液对表皮葡萄球菌ATCC 35984和临床分离株的MIC值分别为4,2μg/mL,MBC值分别为16,8μg/mL。与未加氟苯尼考注射液的菌液相比，0.5μg/mL氟苯尼考注射液可显著抑制表皮葡萄球菌生长，0.25μg/mL氟苯尼考可显著抑制其生物被膜的形成(P<0.05),浓度>2μg/mL时抑制效果极显著(P<0.01),呈浓度依赖性；氟苯尼考注射液可降低表皮葡萄球菌细胞表面疏水性，呈浓度依赖性。当氟苯尼考注射液浓度为1/2 MIC时，可在体外显著抑制表皮葡萄球菌ATCC 35984生长(P<0.05);当浓度高于1 MIC时，可极显著抑制表皮葡萄球菌ATCC 35984和临床分离株生长(P<0.01)。当... 相似文献
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用平衡透析法测定克蚕菌与家蚕血浆蛋白结合率 ,克蚕菌浓度用标准曲线法求出。克蚕菌血浆浓度在5 74～ 188.6 7μg/mL时血浆蛋白结合率为 5 3 4 0 %～ 2 9.2 8%。结合参数的最大结合力 ( β)为 5 88× 10 -6mol/g;解离常数 (Kdp)为 1 0 8× 10 -4mol/L ;结合常数 (K)为 9 82× 10 3 L/mol;结合部位 (N)为 0 9132。克蚕菌与家蚕血浆蛋白具有单一类型的结合部位 ,药物血浆浓度升高结合率下降。 相似文献
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为了研究氟苯尼考与乳酸诺氟沙星在治疗鸡大肠杆菌病时的相互作用,本试验采用肉汤稀释棋盘法,用氟苯尼考与乳酸诺氟沙星对鸡致病性大肠杆菌进行了体外联合药敏试验。氟苯尼考与乳酸诺氟沙星单独用药时的最小抑菌浓度(MIC)分别为16μg/mL、0.8μg/mL,联合用药的MIC分别为16μg/mL、3.2μg/mL,经计算FIC指数为5。结果表明,氟苯尼考与乳酸诺氟沙星联合用药对鸡大肠杆菌呈现拮抗作用。 相似文献
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高效液相色谱法测定猪血浆中氟苯尼考的含量 总被引：2，自引：0，他引：2
建立了猪血浆中氟苯尼考含量测定的高效液相色谱法(HPLC)。采用C18色谱柱(4.6mm×250mm,5μm),以乙腈-水(24:76)为流动相,流速1.0mL/min,检测波长223nm,进样量20μL,柱温为25℃。氟苯尼考浓度在0.1～16μg/mL时,标准曲线线性关系良好(r0.9998),无杂质干扰,回收率高于94%,批内变异系数和批间变异系数均低于5%。本方法操作简便,分析快速,结果准确,适用于猪血浆中氟苯尼考含量的测定及相关药代动力学研究。 相似文献
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为研究黄芪乙醇粗提物对猫肾(Crandell-Rees Feline Kidney, CRFK)细胞的毒性,利用50%(体积分数)乙醇,超声(50℃,280 W)提取黄芪粉末,制得黄芪乙醇粗提物(简称“粗提物AME”);在初步分析粗提物AME的3类组成物质含量基础上,测定在不同质量浓度粗提物AME作用下的CRFK细胞活力、中性红吞噬能力及裂解液总蛋白质量浓度,并分析了粗提物AME对过氧化氢处理(氧化诱导)的细胞活力及总氧自由基水平的影响。研究结果显示：粗提物AME中多糖、总多酚和总蛋白质量分数分别为(53.07±1.73)%、(13.80±0.78)%和(0.13±0.01)%;在作用96 h内,高质量浓度粗提物AME(2 000μg/mL)使CRFK细胞活力降低,低质量浓度粗提物AME(50～1 000μg/mL)使CRFK细胞活力增强;粗提物AME添加质量浓度≥500μg/mL时,CRFK细胞中性红吞噬率降低,CRFK细胞裂解液总蛋白质量浓度也显著降低;50～2 000μg/mL的粗提物AME对由过氧化氢诱导的CRFK细胞活力下降具有显著抑制作用,且对胞内总氧自由基具有清除作用。 相似文献
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为研究磺胺嘧啶钠与兔血浆中血浆蛋白结合情况,采用平衡透析法和超滤法模拟磺胺嘧啶钠与血浆蛋白结合过程,高效液相色谱法测定透析内液及外液中药物浓度,计算磺胺嘧啶钠血浆蛋白结合率。结果表明:血浆中其他成分对测定无干扰,各待测物在选择浓度范围内线性关系均良好,精密度及稳定性结果均符合方法学要求。透析外液线性范围为0.5~20μg/m L,透析内液的线性范围为1~100μg/m L,提取回收率在78.67%~98.36%之间,日内、日间变异均小于10%,磺胺嘧啶钠的体外血浆蛋白结合率平均为32.47%,体内血浆蛋自结合率平均为36.43%。本试验建立的方法简单,灵敏、专属性强,磺胺嘧啶钠在兔血浆中蛋白结合率较低,且具有浓度依赖性。 相似文献
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本试验采集43头苏淮猪的血液样本,探究利用高效液相色谱法(HPLC)测定血浆和全血五羟色胺(5-HT)的色谱条件,测定了血浆、全血和血小板中5-HT的水平,并对它们进行了相关性分析。结果:猪血浆中5-HT浓度为(45.35±2.23)μg/mL,血小板中5-HT含量为(131.31±7.86)pg/10^9,全血中5-HT浓度为(296.87±12.81)μg/mL;相关性分析显示,血小板5-HT浓度与全血5-HT浓度存在极显著正相关性(r=0.849,P<0.01),血小板5-HT浓度与血小板数量存在极显著负相关性(r=-0.549,P<0.01),但血浆5-HT浓度与全血5-HT浓度、血小板5-HT浓度无显著相关性(P>0.05)。综上,高效液相色谱法可用于测定猪血浆、全血、血小板中5-HT的水平,血小板5-HT含量与全血5-HT浓度和血小板数量之间均存在极显著相关性。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献