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本研究应用放射免疫分析法,测定了关中驴产后期(n=18)和发情期(n=6)血浆17β-雌二醇(E_2)、孕酮(P)和辜酮(T)的浓度。测定结果表明,产后第一次发情前血浆E_2浓度较低(12.22±1.92pg/ml),第一次发情期逐渐上升,排卵前第一天和第二天达到较高水平(19.48±2.16pg/ml和20.41±3.27pg/ml,±SE,P<0.05),排卵后降至基础浓度。产后第一次发情前血浆P浓度较低(2.74±0.51ng/ml),排卵后逐渐上升,第四天和第五天显著和极显著高于排卵前P水平,血浆值为6.19±0.66ng/ml和8.09±1.09ng/ml(P<0.05和P<0.01)。产后第一次发情前血浆T浓度较高,发情期下降,排卵后逐渐上升,第五天和第六天显著和极显著高于发情期T水平,分别为313.11±37.57pg/ml和356.73±46.37pg/ml(P<0.05和P<0.01)。产后第一次排卵前P/E_2和P/T比值均较低,排卵后上升,第五天分别达到861和25.83。产后第一次发情前T/E_2比值较高,发情期下降,排卵后上升,第五天达到33.34。排卵前后间隔6小时采样的分析结果表明,排卵前第30～24小时,E_2浓度急剧上升达到峰值。排卵后P浓度于第78～96小时达到较高水平。血浆E_2与P、E_2与T均呈中等负相关(r=-0.42和r=-0.41)。P与T为极显著正相关(r=0.92)。周期性发情期血浆E_2、P、T浓度的分泌范型和产后第一次发情期基本相似。 相似文献
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[目的]对广西水牛发情周期唾液和血液中雌激素(E2)和孕酮(P4)的浓度变化规律及相关性进行了分析,为进一步探讨水牛发情规律来指导生产提供依据。[方法]采用酶联免疫分析方法(ELISA)测定发情母水牛血液和唾液中E2(雌二醇)和P4(孕酮)的浓度变化,并对血液和唾液的激素变化规律进行了相关性分析。[结果]广西水牛血液和唾液中的E2和P4呈波动性变化,结果表明,发情前期,唾液中P4浓度一直维持在6.57.1ng/mL,第13天达到11.09ng/mL以上,随后快速下降。唾液中E2浓度在发情前期第35天出现一个峰值178.528pg/mL,在1417d唾液中E2浓度显著升高,出现第二个峰值达到120.57pg/mL。唾液E2和P4浓度的变化趋势和血清E2和P4浓度的变化趋势基本一致,相关性较好。母水牛唾液和血清样品的P4浓度相关性显著(R=0.4080;P0.05),母水牛唾液和血清标本的E2浓度相关性显著(R=0.5113;P0.05)母水牛唾液E2和P4浓度相关性非常显著(R=0.7754;P0.01)。[结论]唾液和血液中的这两种激素水平起伏变化基本趋于一致,其相关性显著,可以作为监测水牛发情及预测排卵的可靠指标之一。 相似文献
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研究利用3种不同的方法超数排卵处理沼泽型水牛,比较研究不同方法处理时水牛血清雌二醇(E2)、孕酮(P4)浓度变化规律。结果表明:进口FolltropinR○-V、国产FSH和PMSG超数排卵处理沼泽型水牛,血清E2浓度峰值分别出现在氯前列烯醇(PGc)处理后的48 h([142.45±94.66)pg/mL]、72 h([87.78±29.62)pg/mL]、48 h([126.38±92.33)pg/mL];血液中P4浓度最低值分别出现在PGc处理后的48 h([0.76±0.21)pg/mL]、24 h([1.18±0.12)pg/mL]和144 h([0.82±0.06)pg/mL]。 相似文献
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《养殖与饲料．饲料世界》2020,(9)
本试验从15只处于发情前期母犬中随机选取6只母犬,检测其促黄体生成素(LH)、促卵泡素(FSH)和孕酮(P4)3种激素,实现对母犬发情期连续的监测过程。采用静脉采血,抗凝低温离心后分别测定促黄体生成素(LH)、促卵泡素(FSH)、孕酮(P4)的含量,促黄体生成素采用人促黄体生成激素放射免疫分析药盒的方法测定,促卵泡素采用人促滤泡激素放射免疫分析药盒的方法测定,孕酮采用孕酮放射免疫分析药盒的方法测定。测定结果显示:母犬发情期的促黄体生成素的变化范围为5.16～6.82 ng/mL(除峰值外),其峰值平均值是116.71ng/mL;母犬发情期的促卵泡素的变化范围为3.18～4.78 ng/mL(除峰值外),其峰值平均值是43.56 ng/mL;母犬发情期的孕酮的变化范围为0.199 1～8.506 3 ng/mL,其峰值平均值是8.506 3 ng/mL。母犬在发情时,可出现LH释放波,并在LH开始释放后24 h内或开始发情的1～2 d内排卵。 相似文献
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利用放射免疫方法测量雌性黑熊尿液中雌二醇含量,探索不同时期尿液中雌二醇含量变化规律.结果表明雌性黑熊尿液中雌二醇含量在发情间期平均为(0.014±0.010)ng/mgCr,发情前期逐渐升高平均为(0.026±0.023)ng/mgCr,在发情期达到峰值平均为(0.053±0.037)ng/mgCr,其中最大值可达0.393 ng/mgCr,在发情后期逐渐下降平均为(0.012±0.012)ng/mgCr.且发情期雌二醇含量与发情后期、发情间期差异极显著(P＜0.01),与发情前期差异显著(P≤0.05),发情前期与发情后期差异显著(P≤0.05),其余不显著(P＞0.05). 相似文献
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牦牛发情集中在7月中旬至9月下旬。发情周期平均20.95±5.13d。发情持续时间在24h以内的占69%超过1d的占31%最长可达96h。发情率为66.7%(n=225),其中空怀牛和带犊牛分别为92.1%和50%。未带犊牛发情期17-β雌二醇平均为231±100pg/mL,黄体期孕酮平均为0.43±0.07ng/mL。每日挤乳1次的带犊牛,孕酮及17-β二醇分别为0.26±0.08ng/mL和61±26.0pg/mL。 相似文献
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繁殖期雌性大熊猫尿中激素变化的研究 总被引：2，自引：0，他引：2
用ELISA法对39只次发情雌性大熊猫尿中雌酮(E1G)和19只次产前大熊猫、6只次假孕大熊猫尿中孕酮(P4)的含量进行测定、统计分析,结果表明:(1)雌性成体大熊猫,发情期多在2—5月份,尿中E1G/Cr由基础值<12ng/mg开始上升,达峰值需8—15d,平均12d。E1G/Cr峰值>60ng/mg,峰值下降12h内进行首次本交、人工授精或两者交替配种,受孕率达100%;峰值下降12—24h内进行首次本交、人工授精或两者交替配种,受孕率达83 3%;峰值下降24h后进行首次配种,受孕率为0。E1G/Cr峰值<60ng/mg的发情雌性大熊猫,无论在峰前、峰值下降时或峰后进行首次配种,受孕率都为0。(2)大熊猫产仔季节一般多在7—9月份,妊娠期多为108—184d,平均150d。有妊娠行为表现时P4/Cr由基础值<12ng/mg开始上升,达峰值时平均需30d,P4/Cr峰值>60ng/mg。大熊猫产前9—18d出现P4/Cr峰值,然后急剧下降,达产仔平均需14d。(3)假孕大熊猫由配种到假孕结束所需时间与真孕无显著性差异。在整个假孕期间,P4/Cr波动性大,峰值不太明显,假孕行为表现持续时间平均为41d。 相似文献
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应用放射免疫分析法测定了6只西农莎能奶山羊发情周期中的乳汁孕酮水平。发情期(0～1天,发情=0天)的孕酮水平最低(0.83±0.28ng/ml),发情第5天后不断上升,分别于第11、15、17天达峰值(23.4、34.2、15.9、32.2、12.6和20.5ng/ml),此后又逐渐下降,发情前1天降至4ng/ml。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献