共查询到20条相似文献，搜索用时 78 毫秒
1.
为了寻找筛选猪瘟抗体阴性猪更简便的方法,本试验应用猪瘟中和试验(SN)、阻断ELISA和正向间接血凝(IHA)三种方法分别对170份未经猪瘟活疫苗免疫的仔猪血清进行猪瘟抗体检测。结果显示,SN、ELISA和IHA检测出的猪瘟阴性血清分别为144份、150份和131份,表明三种检测方法有较好的符合率,其中SN与ELISA的检测符合率为90.59%,SN与IHA的检测符合率为82.94%,而ELISA法与另两种方法比较阴性符合率最高。本结果表明,ELISA法具有较高的敏感性,适合应用于猪瘟活疫苗安全检验抗体阴性猪的筛选。 相似文献
2.
为建立检测牛传染性鼻气管炎病毒(IBRV)血清抗体的阻断ELISA方法,本研究以经蔗糖密度梯度离心法纯化的IBRV作为免疫原制备1株单克隆抗体(MAb),命名为cp-1-1。经间接ELISA、IFA和western blot鉴定,该MAb与IBRV呈阳性反应,与牛病毒性腹泻病毒(BVDV)及牛副流感病毒3型(BPIV3)呈阴性反应,具有较强的特异性。质谱分析结果显示MAb cp-1-1识别的表位位于IBRV VP8蛋白。以纯化的IBRV作为包被抗原、MAb cp-1-1作为检测抗体,建立检测IBRV血清抗体的阻断ELISA方法。该检测方法的抗原包被量为0.89μg/孔,样品稀释度为12,检测抗体MAb量为1.3μg/孔,二抗稀释度为15000。利用50份IBRV抗体呈弱阳性的牛血清(中和抗体效价为14~116)作为标准参考血清,确定该检测方法的阻断率Cut Off值为52.06%,即阻断率高于52.06%时判为阳性,低于52.06%时判为阴性。阻断ELISA方法特异性试验显示仅IBRV阳性血清检测为阳性,而BVDV、BPIV3、牛腺病毒3型(BADV-3)和O型口蹄疫病毒(O-FMDV)阳性牛血清均检测为阴性,表明该方法具有较强的特异性;该方法可检测的最低中和抗体效价为14,与病毒中和试验的敏感性一致,表明该方法具有较高的敏感性;重复性试验显示该方法批内、批间变异系数均小于10%,显示较好的重复性。对130份现地牛血清检测结果显示,该方法与病毒中和试验的符合率为98.46%。用该方法对某牛场接种IBRV灭活疫苗的牛血清进行检测,抗体阳性率为99.51%(205/206)。另外,采用该方法对我国8个省(市、自治区)的801份牛血清进行检测,IBRV的抗体阳性率为41.6%(333/801)。本研究建立的阻断ELISA方法可以用于IBRV疫苗免疫监测和血清流行病学调查,为我国IBR的防控提供技术支持。 相似文献
3.
竞争ELISA检测猪呼吸与繁殖综合征病毒抗体研究 总被引：5，自引：0，他引：5
应用抗PRRSN蛋的单克隆抗体建立了竞争ELISA检测技术,与美国IDEXX公司的间接ELISA检测试剂盒对比检测了30份SPF猪血清,29头SPF猪人工接种呼吸与繁殖综合征病毒后于5d、12d、29d采集的猪血清共87份,临床血清95份,以及其他相关病毒的阳性血清。实验证明,竞争ELISA的检测阴阳性限值为30%,检测特异性为100%,在人工攻PRRSV病毒第五天的猪血清中,可检测到抗PRRSVN蛋白抗体,12dpi,阳性检出率为93%,29dpi,阳性检出率为100%,与IDEXXPRRSVELISA试剂盒进行比对,结果表明,二种检测方法检测美洲株PRRSV抗体的符合率为90%以上,但检测欧洲株抗体符合率较低。建立的竞争ELISA检测技术对于PRRSV美洲株抗体的早期诊断有非常重要的意义。 相似文献
4.
5.
本研究建立的检测狂犬病抗体的夹心阻断ELISA,直接利用未经提纯的病毒悬液代替提纯的抗原,并仅用一种酶标抗体,测定了9种动物的血清.本方法敏感度的95%可信限为0.0013～0.0071IU/mL,比小鼠中和试验和微量免疫酶试验均敏感.按阻断50%判定血清ELISA的阴阳性,9种动物的1134份血清中有91份阳性,其中发病点的牛、猪、犬、家鼠血清的阳性率均在10%以上.根据对一定量的抗原阻断率相同时,抗体浓度一致的原理,测定了7种动物的185份血清效价,结果狂犬病抗体浓度在0.01IU/mL以上的为63份.利用夹心阻断ELISA和小鼠中和试验测定了7种动物的23份血清,阳性份数分别为12和11.两种方法均证明家鼠血清中有狂犬病抗体.本研究表明,测定狂犬病抗体的夹心阻断ELISA,简便、敏感、快速,可用于流行病学调查. 相似文献
6.
7.
本研究旨在建立一种非洲猪瘟病毒(ASFV)p72蛋白阻断ELISA抗体检测方法。以纯化的重组p72蛋白作为包被抗原,HRP标记的6E5抗体作为阻断抗体,通过对反应条件进行优化,建立了基于ASFV p72蛋白的阻断ELISA方法。用建立的阻断ELISA方法检测119份临床阴性血清,计算血清阻断率确定临界值,确定了该方法的判定标准：当阻断率PI≥50%时判定为ASFV抗体阳性;PI≤40%时判定为ASFV抗体阴性;当阻断率介于两者之间时,判定为可疑。该方法与猪伪狂犬病病毒、猪繁殖与呼吸综合征病毒、猪瘟病毒、猪圆环病毒2型、A型塞内卡病毒、猪口蹄疫病毒、猪大肠杆菌、猪多杀性巴氏杆菌、副猪格拉瑟菌和猪胸膜肺炎放线杆菌的抗体阳性血清无交叉反应,批间、批内试验的变异系数均小于15%。用该方法与商品化ASFV抗体检测试剂盒同时检测447份猪临床血清样品,两种方法的相对敏感性和相对特异性分别为95.3%和94.5%,符合率为94.9%。本研究建立的阻断ELISA方法具有较高的敏感性和良好的特异性,可用于ASFV感染诊断和流行病学调查。 相似文献
8.
分别采用商品化的酶联免疫吸附试验(ELISA)抗体检测试剂盒和血凝抑制(HI)试验方法对153份血清样本进行禽流感病毒(AIV)的H5抗体检测,比较间接ELISA和HI两种方法检测结果的相关性。研究结果表明,间接ELISA和HI两种方法呈较好的相关性,二者间的相关系数为0.765。间接ELISA和HI两种方法检测AIV抗体的阳性符合率为95.8%,阴性符合率为87.2%,总体符合率为96.7%。间接ELISA方法是一种敏感、特异和快速的血清学诊断方法,可以用于临床样本的大规模检测。 相似文献
9.
通过正向间接血凝试验和酶联免疫吸附试验（阻断ELISA）对3个规模化猪场提供的350份血清样品进行猪瘟免疫效果平行对比检测，结果：A猪场120份，正向间接血凝试验检测免疫合格为95份，免疫合格率为79.2%；阻断ELISA检测免疫合格为92份，免疫合格率76.7%。B猪场120份，正向间接血凝试验检测免疫合格为108份，免疫合格率为90.0%；阻断ELISA检测免疫合格为104份，免疫合格率为86.7%。C猪场110份，正向间接血凝试验检测免疫合格为58份，免疫合格率为52.7%；阻断ELISA检测免疫合格为55份，免疫合格率为50.0%。 相似文献
10.
检测猪乙型脑炎病毒抗体间接ELISA方法的建立与应用 总被引：3，自引：2，他引：1
以乙型脑炎病毒(JEV)弱毒疫苗株作为诊断抗原,对ELISA反应条件进行优化,初步建立了检测猪乙型脑炎病毒血清抗体的间接ELISA方法。应用该法检测从江苏、安徽、山东、浙江4省部分地区收集到的1089份血样,对华东地区猪乙型脑炎血清流行病学进行初步调查。结果JEV抗体阳性率为68.2%,其中,种猪JEV抗体阳性率为82.1%,商品猪JEV抗体阳性率为62.6%。从中随机抽取135份血样与国产商品化试剂盒进行比较,间接ELISA方法的特异性和敏感性分别为92%和96.4%,2种方法的符合率为95.6%;与NS1蛋白包被建立的ELISA比较,两者的符合率为97.7%;同时,本法的阳性检出率显著高于临床普遍使用的乳胶凝集试验结果。由此表明,本试验建立的间接ELISA方法具有较高的敏感性和特异性,适于大规模猪乙型脑炎血清流行病学调查。 相似文献
11.
12.
魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
13.
14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
15.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
16.
17.
18.
Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
19.
20.
以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献